ID   EGR4_HUMAN              Reviewed;         589 AA.
AC   Q05215; B2RAE3; G3V1T5; Q2Z1P5;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2013, sequence version 3.
DT   24-JAN-2024, entry version 190.
DE   RecName: Full=Early growth response protein 4;
DE            Short=EGR-4;
DE   AltName: Full=AT133;
GN   Name=EGR4;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8504297; DOI=10.1093/hmg/2.4.367;
RA   Holst C., Skerka C., Lichter P., Bialonski A., Zipfel P.F.;
RT   "Genomic organization, chromosomal localization and promoter function of
RT   the human zinc-finger gene pAT133.";
RL   Hum. Mol. Genet. 2:367-372(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA   Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA   Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA   Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA   Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA   Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA   Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA   Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA   Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA   Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA   Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA   Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA   Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA   Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA   Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA   Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT   4.";
RL   Nature 434:724-731(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 49-589.
RX   PubMed=1658795; DOI=10.1073/pnas.88.22.10079;
RA   Mueller H.-J., Skerka C., Bialonski A., Zipfel P.F.;
RT   "Clone pAT133 identifies a gene that encodes another human member of a
RT   class of growth factor-induced genes with almost identical zinc-finger
RT   domains.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:10079-10083(1991).
CC   -!- FUNCTION: Transcriptional regulator. Recognizes and binds to the DNA
CC       sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target
CC       genes whose products are required for mitogenesis and differentiation
CC       (By similarity). {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q05215; Q9NYB9-2: ABI2; NbExp=3; IntAct=EBI-19949420, EBI-11096309;
CC       Q05215; O95817: BAG3; NbExp=3; IntAct=EBI-19949420, EBI-747185;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- INDUCTION: By PHA/PMA or by serum.
CC   -!- SIMILARITY: Belongs to the EGR C2H2-type zinc-finger protein family.
CC       {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-104 is the initiator.
CC       Some orthologous sequences cannot be extended. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAX88903.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAG36840.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=CAA42698.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=CAA49214.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X69438; CAA49214.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AK314154; BAG36840.1; ALT_INIT; mRNA.
DR   EMBL; AC010913; AAX88903.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CH471053; EAW99734.1; -; Genomic_DNA.
DR   EMBL; X60104; CAA42698.1; ALT_FRAME; mRNA.
DR   PIR; A41537; A41537.
DR   RefSeq; NP_001956.3; NM_001965.3.
DR   AlphaFoldDB; Q05215; -.
DR   SMR; Q05215; -.
DR   BioGRID; 108281; 4.
DR   IntAct; Q05215; 2.
DR   STRING; 9606.ENSP00000445626; -.
DR   iPTMnet; Q05215; -.
DR   PhosphoSitePlus; Q05215; -.
DR   BioMuta; EGR4; -.
DR   DMDM; 485956294; -.
DR   MassIVE; Q05215; -.
DR   PaxDb; 9606-ENSP00000445626; -.
DR   PeptideAtlas; Q05215; -.
DR   Antibodypedia; 16536; 140 antibodies from 27 providers.
DR   DNASU; 1961; -.
DR   Ensembl; ENST00000545030.1; ENSP00000445626.1; ENSG00000135625.8.
DR   GeneID; 1961; -.
DR   KEGG; hsa:1961; -.
DR   UCSC; uc010yrj.3; human.
DR   AGR; HGNC:3241; -.
DR   CTD; 1961; -.
DR   DisGeNET; 1961; -.
DR   GeneCards; EGR4; -.
DR   HGNC; HGNC:3241; EGR4.
DR   HPA; ENSG00000135625; Tissue enriched (brain).
DR   MIM; 128992; gene.
DR   neXtProt; NX_Q05215; -.
DR   OpenTargets; ENSG00000135625; -.
DR   PharmGKB; PA27676; -.
DR   VEuPathDB; HostDB:ENSG00000135625; -.
DR   eggNOG; KOG1721; Eukaryota.
DR   GeneTree; ENSGT00940000162199; -.
DR   InParanoid; Q05215; -.
DR   OMA; LYKWGGC; -.
DR   OrthoDB; 2912670at2759; -.
DR   PhylomeDB; Q05215; -.
DR   TreeFam; TF318980; -.
DR   PathwayCommons; Q05215; -.
DR   Reactome; R-HSA-9031628; NGF-stimulated transcription.
DR   SignaLink; Q05215; -.
DR   BioGRID-ORCS; 1961; 22 hits in 1161 CRISPR screens.
DR   GeneWiki; EGR4; -.
DR   GenomeRNAi; 1961; -.
DR   Pharos; Q05215; Tbio.
DR   PRO; PR:Q05215; -.
DR   Proteomes; UP000005640; Chromosome 2.
DR   RNAct; Q05215; Protein.
DR   Bgee; ENSG00000135625; Expressed in type B pancreatic cell and 89 other cell types or tissues.
DR   ExpressionAtlas; Q05215; baseline and differential.
DR   GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR   GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; NAS:ProtInc.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; TAS:ProtInc.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 3.30.160.60; Classic Zinc Finger; 3.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   PANTHER; PTHR23235:SF58; EARLY GROWTH RESPONSE PROTEIN 4; 1.
DR   PANTHER; PTHR23235; KRUEPPEL-LIKE TRANSCRIPTION FACTOR; 1.
DR   Pfam; PF00096; zf-C2H2; 3.
DR   SMART; SM00355; ZnF_C2H2; 3.
DR   SUPFAM; SSF57667; beta-beta-alpha zinc fingers; 2.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
DR   Genevisible; Q05215; HS.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..589
FT                   /note="Early growth response protein 4"
FT                   /id="PRO_0000047128"
FT   ZN_FING         483..507
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         513..535
FT                   /note="C2H2-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         541..563
FT                   /note="C2H2-type 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          29..101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          383..411
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          436..466
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        75..90
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        441..456
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        211
FT                   /note="I -> V (in Ref. 5; CAA42698 and 1; CAA49214)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        291
FT                   /note="A -> R (in Ref. 5; CAA42698 and 1; CAA49214)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        446
FT                   /note="V -> M (in Ref. 2; BAG36840)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        530
FT                   /note="T -> S (in Ref. 1; CAA49214)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   589 AA;  61623 MW;  EFB5A743541A07D7 CRC64;
     MAVARGVGSP EPAPPQLYKW GGCGLGEPGS ALERRGAAAR GRCGRARAPR LPDSFPRGEC
     PKPGARAPRS VRCGEPLPPA SPPPARPQAQ RARPRAPHSR RRAMLHLSEF SEPDALLVKS
     TEGCCAEPSA ELPRLPARDA PAATGYPGAG DFLSWALNSC GASGDLADSC FLEGPAPTPP
     PGLSYSGSFF IQAVPEHPHD PEALFNLMSG ILGLAPFPGP EAAASRSPLD APFPAGSDAL
     LPGPPDLYSP DLGAAPFPEA FWEASPCAGA PSQCLYEPQL SPPDVKPGLR APPASPALDA
     VSAFKGPYAP WELLSVGAPG NCGSQGDYQA APEARFPVIG TKIEDLLSIS CPAELPAVPA
     NRLYPSGAYD AFPLAPGDLG EGAEGLPGLL TPPSGEGGSS GDGGEFLAST QPQLSPLGLR
     SAAAADFPKP LVADIPGSSG VAAPPVPPPP PTPFPQAKAR RKGRRGGKCS TRCFCPRPHA
     KAFACPVESC VRSFARSDEL NRHLRIHTGH KPFQCRICLR NFSRSDHLTT HVRTHTGEKP
     FACDVCGRRF ARSDEKKRHS KVHLKQKARA EERLKGLGFY SLGLSFASL
//