Q05215 (EGR4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Early growth response protein 4
Short name=EGR-4

Alternative name(s):
AT133

Gene names

Name:

EGR4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCGGGGGCG-3' (GSG). Activates the transcription of target genes whose products are required for mitogenesis and differentiation By similarity.
Subcellular location	Nucleus Probable.
Induction	By PHA/PMA or by serum.
Sequence similarities	Belongs to the EGR C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.
Caution	It is uncertain whether Met-1 or Met-104 is the initiator. Some orthologous sequences can not be extended.
Sequence caution	The sequence AAX88903.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG36840.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAA42698.1 differs from that shown. Reason: Frameshift at position 66. The sequence CAA49214.1 differs from that shown. Reason: Frameshift at position 66.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of cell proliferation Traceable author statement Ref.5. Source: ProtInc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Non-traceable author statement PubMed 1584812. Source: ProtInc zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Early growth response protein 4		PRO_0000047128
Regions
	Zinc finger	483 – 507	25	C2H2-type 1
	Zinc finger	513 – 535	23	C2H2-type 2
	Zinc finger	541 – 563	23	C2H2-type 3
	Compositional bias	175 – 455	281	Pro-rich
Experimental info
	Sequence conflict	211	1	I → V in CAA42698. Ref.5
	Sequence conflict	211	1	I → V in CAA49214. Ref.1
	Sequence conflict	291	1	A → R in CAA42698. Ref.5
	Sequence conflict	291	1	A → R in CAA49214. Ref.1
	Sequence conflict	446	1	V → M in BAG36840. Ref.2
	Sequence conflict	530	1	T → S in CAA49214. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q05215 [UniParc]. Last modified May 1, 2013. Version 3. Checksum: EFB5A743541A07D7	FASTA	589	61,623
	10 20 30 40 50 60 MAVARGVGSP EPAPPQLYKW GGCGLGEPGS ALERRGAAAR GRCGRARAPR LPDSFPRGEC 70 80 90 100 110 120 PKPGARAPRS VRCGEPLPPA SPPPARPQAQ RARPRAPHSR RRAMLHLSEF SEPDALLVKS 130 140 150 160 170 180 TEGCCAEPSA ELPRLPARDA PAATGYPGAG DFLSWALNSC GASGDLADSC FLEGPAPTPP 190 200 210 220 230 240 PGLSYSGSFF IQAVPEHPHD PEALFNLMSG ILGLAPFPGP EAAASRSPLD APFPAGSDAL 250 260 270 280 290 300 LPGPPDLYSP DLGAAPFPEA FWEASPCAGA PSQCLYEPQL SPPDVKPGLR APPASPALDA 310 320 330 340 350 360 VSAFKGPYAP WELLSVGAPG NCGSQGDYQA APEARFPVIG TKIEDLLSIS CPAELPAVPA 370 380 390 400 410 420 NRLYPSGAYD AFPLAPGDLG EGAEGLPGLL TPPSGEGGSS GDGGEFLAST QPQLSPLGLR 430 440 450 460 470 480 SAAAADFPKP LVADIPGSSG VAAPPVPPPP PTPFPQAKAR RKGRRGGKCS TRCFCPRPHA 490 500 510 520 530 540 KAFACPVESC VRSFARSDEL NRHLRIHTGH KPFQCRICLR NFSRSDHLTT HVRTHTGEKP 550 560 570 580 FACDVCGRRF ARSDEKKRHS KVHLKQKARA EERLKGLGFY SLGLSFASL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization, chromosomal localization and promoter function of the human zinc-finger gene pAT133." Holst C., Skerka C., Lichter P., Bialonski A., Zipfel P.F. Hum. Mol. Genet. 2:367-372(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Clone pAT133 identifies a gene that encodes another human member of a class of growth factor-induced genes with almost identical zinc-finger domains." Mueller H.-J., Skerka C., Bialonski A., Zipfel P.F. Proc. Natl. Acad. Sci. U.S.A. 88:10079-10083(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 49-589.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69438 Genomic DNA. Translation: CAA49214.1. Frameshift. AK314154 mRNA. Translation: BAG36840.1. Different initiation. AC010913 Genomic DNA. Translation: AAX88903.1. Different initiation. CH471053 Genomic DNA. Translation: EAW99734.1. X60104 mRNA. Translation: CAA42698.1. Frameshift.
IPI	IPI00289101.
PIR	A41537.
UniGene	Hs.3052.
3D structure databases
ProteinModelPortal	Q05215.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000419687.
Polymorphism databases
DMDM	125987824.
Proteomic databases
PaxDb	Q05215.
PRIDE	Q05215.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000436467; ENSP00000419687; ENSG00000135625.
UCSC	uc010yrj.2. human.
Organism-specific databases
GeneCards	GC02M073518.
HGNC	HGNC:3241. EGR4.
HPA	HPA028983.
MIM	128992. gene.
neXtProt	NX_Q05215.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000112347.
HOVERGEN	HBG005574.
InParanoid	Q05215.
OrthoDB	EOG49P9ZG.
PhylomeDB	Q05215.
Enzyme and pathway databases
Pathway_Interaction_DB	nfat_tfpathway. Calcineurin-regulated NFAT-dependent transcription in lymphocytes. cd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells.
Gene expression databases
ArrayExpress	Q05215.
Bgee	Q05215.
CleanEx	HS_EGR4.
Genevestigator	Q05215.
GermOnline	ENSG00000135625. Homo sapiens.
Family and domain databases
Gene3D	3.30.160.60. 3 hits.
InterPro	IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
SMART	SM00355. ZnF_C2H2. 3 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

EGR4_HUMAN

Accession

Primary (citable) accession number: Q05215
Secondary accession number(s): B2RAE3, G3V1T5, Q2Z1P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	May 1, 2013
Last modified:	May 1, 2013
This is version 114 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.