ID   PPB_LYSEN               Reviewed;         539 AA.
AC   Q05205;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   16-JUN-2009, entry version 42.
DE   RecName: Full=Alkaline phosphatase;
DE            Short=APASE;
DE            EC=3.1.3.1;
DE   Flags: Precursor;
GN   Name=phoA;
OS   Lysobacter enzymogenes.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Lysobacter.
OX   NCBI_TaxID=69;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 149-179;
RP   267-277 AND 341-361.
RC   STRAIN=ATCC 29487 / DSM 2043 / LMG 8762 / UASM 495 / VKM B-2235;
RX   MEDLINE=91310559; PubMed=1856159;
RA   Au S., Roy K.L., von Tigerstrom R.G.;
RT   "Nucleotide sequence and characterization of the gene for secreted
RT   alkaline phosphatase from Lysobacter enzymogenes.";
RL   J. Bacteriol. 173:4551-4557(1991).
CC   -!- CATALYTIC ACTIVITY: A phosphate monoester + H(2)O = an alcohol +
CC       phosphate.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: The C-terminal Pro-sequence may be required for translocation
CC       of the phosphatase across the outer membrane.
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DR   EMBL; X56656; CAA39978.1; -; Genomic_DNA.
DR   PIR; A42467; A42467.
DR   BRENDA; 3.1.3.1; 19052.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004035; F:alkaline phosphatase activity; IEA:EC.
DR   InterPro; IPR004843; M-pesterase.
DR   Pfam; PF00149; Metallophos; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Secreted; Signal.
FT   SIGNAL        1     29       Potential.
FT   PROPEP       30    148
FT                                /FTId=PRO_0000023994.
FT   CHAIN       149    429       Alkaline phosphatase.
FT                                /FTId=PRO_0000023995.
FT   PROPEP      430    539       Potential.
FT                                /FTId=PRO_0000023996.
SQ   SEQUENCE   539 AA;  56490 MW;  C51DC38429936C3E CRC64;
     MNLSPSRTPI CAALAAALLG AAALAPAHAA QRILQLSEDT THSKPVSAAS ALRGTPLAKA
     GAADRVCEAG AKWLRVGFKQ LKLAGYDSLV LTSSGGDKLV FEGQHWNQRS FTTRPLRGEC
     VDIQPYFSQP DSAFQLDRYD YSTVALDKAT VVVAGAGDIC DTSGNACQGT SDLIVSINPT
     AVFTAGDNAY NSGTLSEYNS RYAPTWGRFK ALTSPSPGNH DYSTTGAKGY FDYFNGSGNQ
     TGPAGDRSKG YYSWDVGDWH FVSLNTMSGG TVAQAQIDWL KADLAANTKP CTAAYFHHPL
     LSRGSYSGYS QVKPFWDALY AAKADLVLVG HDHNYQRYGK MNPDKAAASD GIRQVLVGTG
     GRAFYGISGS HALLEASNDS TFGVLKLTLS ATGYTGDFVP RAGSSYTDHF TGTCNKGSGN
     PPTQTLTLNS VRDVTVKSGG SRDNGATLYA DGSDGGQVLR GLMAWNVSSA AGKTLTGAQV
     KLQVSDRSTG TYDLYRAGAA WTEANASYSG VSLGSKIGSV VPSATGAQSI ALNAAGFSW
//