Reviewed, UniProtKB/Swiss-Prot Q05205 (PPB_LYSEN)

Names and origin

Protein names

Recommended name:
    Alkaline phosphatase
      Short name=APASE
    EC=3.1.3.1

Gene names

Name:

phoA

Organism

Lysobacter enzymogenes

Taxonomic identifier

69 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Lysobacter

Protein attributes

Sequence length	539 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subcellular location	Secreted.
Post-translational modification	The C-terminal Pro-sequence may be required for translocation of the phosphatase across the outer membrane.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alkaline phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Propeptide	30 – 148	119			PRO_0000023994
	Chain	149 – 429	281	Alkaline phosphatase		PRO_0000023995
	Propeptide	430 – 539	110	Potential		PRO_0000023996

Sequences

	Sequence		Length	Mass (Da)
	Q05205-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C51DC38429936C3E	FASTA	539	56,490
	10 20 30 40 50 60 MNLSPSRTPI CAALAAALLG AAALAPAHAA QRILQLSEDT THSKPVSAAS ALRGTPLAKA 70 80 90 100 110 120 GAADRVCEAG AKWLRVGFKQ LKLAGYDSLV LTSSGGDKLV FEGQHWNQRS FTTRPLRGEC 130 140 150 160 170 180 VDIQPYFSQP DSAFQLDRYD YSTVALDKAT VVVAGAGDIC DTSGNACQGT SDLIVSINPT 190 200 210 220 230 240 AVFTAGDNAY NSGTLSEYNS RYAPTWGRFK ALTSPSPGNH DYSTTGAKGY FDYFNGSGNQ 250 260 270 280 290 300 TGPAGDRSKG YYSWDVGDWH FVSLNTMSGG TVAQAQIDWL KADLAANTKP CTAAYFHHPL 310 320 330 340 350 360 LSRGSYSGYS QVKPFWDALY AAKADLVLVG HDHNYQRYGK MNPDKAAASD GIRQVLVGTG 370 380 390 400 410 420 GRAFYGISGS HALLEASNDS TFGVLKLTLS ATGYTGDFVP RAGSSYTDHF TGTCNKGSGN 430 440 450 460 470 480 PPTQTLTLNS VRDVTVKSGG SRDNGATLYA DGSDGGQVLR GLMAWNVSSA AGKTLTGAQV 490 500 510 520 530 KLQVSDRSTG TYDLYRAGAA WTEANASYSG VSLGSKIGSV VPSATGAQSI ALNAAGFSW

References

[1]

"Nucleotide sequence and characterization of the gene for secreted alkaline phosphatase from Lysobacter enzymogenes." Au S., Roy K.L., von Tigerstrom R.G. J. Bacteriol. 173:4551-4557(1991) [PubMed: 1856159] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 149-179; 267-277 AND 341-361. Strain: ATCC 29487 / DSM 2043 / LMG 8762 / UASM 495 / VKM B-2235.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56656 Genomic DNA. Translation: CAA39978.1.
PIR	A42467.
3D structure databases
SMR	Q05205. Positions 170-421.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.1. 19052.
Family and domain databases
InterPro	IPR004843. M-pesterase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPB_LYSEN

Accession

Primary (citable) accession number: Q05205

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 19, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)