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Q05205

- PPB_LYSEN

UniProt

Q05205 - PPB_LYSEN

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Protein
Alkaline phosphatase
Gene
phoA
Organism
Lysobacter enzymogenes
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase (EC:3.1.3.1)
Short name:
APASE
Gene namesi
Name:phoA
OrganismiLysobacter enzymogenes
Taxonomic identifieri69 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeLysobacter

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed prediction
Add
BLAST
Propeptidei30 – 148119
PRO_0000023994Add
BLAST
Chaini149 – 429281Alkaline phosphatase
PRO_0000023995Add
BLAST
Propeptidei430 – 539110 Reviewed prediction
PRO_0000023996Add
BLAST

Post-translational modificationi

The C-terminal Pro-sequence may be required for translocation of the phosphatase across the outer membrane.

Structurei

3D structure databases

ProteinModelPortaliQ05205.

Family & Domainsi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05205-1 [UniParc]FASTAAdd to Basket

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MNLSPSRTPI CAALAAALLG AAALAPAHAA QRILQLSEDT THSKPVSAAS    50
ALRGTPLAKA GAADRVCEAG AKWLRVGFKQ LKLAGYDSLV LTSSGGDKLV 100
FEGQHWNQRS FTTRPLRGEC VDIQPYFSQP DSAFQLDRYD YSTVALDKAT 150
VVVAGAGDIC DTSGNACQGT SDLIVSINPT AVFTAGDNAY NSGTLSEYNS 200
RYAPTWGRFK ALTSPSPGNH DYSTTGAKGY FDYFNGSGNQ TGPAGDRSKG 250
YYSWDVGDWH FVSLNTMSGG TVAQAQIDWL KADLAANTKP CTAAYFHHPL 300
LSRGSYSGYS QVKPFWDALY AAKADLVLVG HDHNYQRYGK MNPDKAAASD 350
GIRQVLVGTG GRAFYGISGS HALLEASNDS TFGVLKLTLS ATGYTGDFVP 400
RAGSSYTDHF TGTCNKGSGN PPTQTLTLNS VRDVTVKSGG SRDNGATLYA 450
DGSDGGQVLR GLMAWNVSSA AGKTLTGAQV KLQVSDRSTG TYDLYRAGAA 500
WTEANASYSG VSLGSKIGSV VPSATGAQSI ALNAAGFSW 539
Length:539
Mass (Da):56,490
Last modified:November 1, 1995 - v1
Checksum:iC51DC38429936C3E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56656 Genomic DNA. Translation: CAA39978.1.
PIRiA42467.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56656 Genomic DNA. Translation: CAA39978.1 .
PIRi A42467.

3D structure databases

ProteinModelPortali Q05205.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence and characterization of the gene for secreted alkaline phosphatase from Lysobacter enzymogenes."
    Au S., Roy K.L., von Tigerstrom R.G.
    J. Bacteriol. 173:4551-4557(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 149-179; 267-277 AND 341-361.
    Strain: ATCC 29487 / DSM 2043 / LMG 8762 / UASM 495 / VKM B-2235.

Entry informationi

Entry nameiPPB_LYSEN
AccessioniPrimary (citable) accession number: Q05205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

External Data

Dasty 3