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Q05201

- EYA_DROME

UniProt

Q05201 - EYA_DROME

Protein

Developmental protein eyes absent

Gene

eya

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Thought to play a role in transcription regulation during organogenesis through its intrinsic protein phosphatase activity. The phosphatase activity was shown in vitro. Appears to function together with So and Dac in eye development. Required for the survival of eye progenitor cells at a critical stage in morphogenesis.

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

    Cofactori

    Binds 1 Mg2+ ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei499 – 4991NucleophileBy similarity
    Metal bindingi499 – 4991MagnesiumBy similarity
    Active sitei501 – 5011Proton donorBy similarity
    Metal bindingi501 – 5011MagnesiumBy similarity
    Metal bindingi730 – 7301MagnesiumBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: FlyBase
    3. protein tyrosine phosphatase activity Source: FlyBase
    4. protein tyrosine phosphatase activity, metal-dependent Source: FlyBase
    5. transcription factor binding Source: FlyBase

    GO - Biological processi

    1. axon guidance Source: FlyBase
    2. Bolwig's organ morphogenesis Source: FlyBase
    3. compound eye development Source: FlyBase
    4. compound eye photoreceptor development Source: FlyBase
    5. eye-antennal disc morphogenesis Source: UniProtKB
    6. gonad development Source: FlyBase
    7. innate immune response Source: FlyBase
    8. larval somatic muscle development Source: FlyBase
    9. male gonad development Source: FlyBase
    10. mesoderm development Source: FlyBase
    11. negative regulation of cell fate specification Source: FlyBase
    12. optic lobe placode formation Source: FlyBase
    13. ovarian follicle cell development Source: FlyBase
    14. peptidyl-tyrosine dephosphorylation Source: GOC
    15. photoreceptor cell axon guidance Source: FlyBase
    16. pole cell migration Source: FlyBase
    17. positive regulation of gene expression Source: FlyBase
    18. positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
    19. protein dephosphorylation Source: FlyBase
    20. response to light stimulus Source: FlyBase
    21. salivary gland morphogenesis Source: FlyBase
    22. spermatogenesis Source: FlyBase
    23. transcription, DNA-templated Source: FlyBase
    24. ventral cord development Source: FlyBase

    Keywords - Molecular functioni

    Activator, Developmental protein, Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Developmental protein eyes absent (EC:3.1.3.48)
    Alternative name(s):
    Protein Clift
    Gene namesi
    Name:eya
    Synonyms:cli
    ORF Names:CG9554
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0000320. eya.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: FlyBase
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi499 – 4991D → N: Highly reduced ectopic eye induction and diminishes degree of ommatidial restoration in eyeless phenotype rescue assay. 2 Publications
    Mutagenesisi676 – 6761S → A: Highly reduced ectopic eye induction and diminishes degree of ommatidial restoration in eyeless phenotype rescue assay. 1 Publication
    Mutagenesisi705 – 7051K → Q: Highly reduced ectopic eye induction and diminishes degree of ommatidial restoration in eyeless phenotype rescue assay. 1 Publication
    Mutagenesisi730 – 7301D → N: Highly reduced ectopic eye induction. 1 Publication
    Mutagenesisi734 – 7341E → Q: Highly reduced ectopic eye induction. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 766766Developmental protein eyes absentPRO_0000218655Add
    BLAST

    Proteomic databases

    PRIDEiQ05201.

    Expressioni

    Gene expression databases

    BgeeiQ05201.

    Interactioni

    Subunit structurei

    Interacts with Dac and So.2 Publications

    Protein-protein interaction databases

    BioGridi60071. 18 interactions.
    DIPiDIP-19253N.
    IntActiQ05201. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05201.
    SMRiQ05201. Positions 493-766.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi46 – 5712Poly-GlnAdd
    BLAST
    Compositional biasi60 – 689Poly-Gln
    Compositional biasi92 – 10817Poly-GlyAdd
    BLAST
    Compositional biasi253 – 2608Poly-Ala
    Compositional biasi268 – 2714Poly-Tyr
    Compositional biasi305 – 3117Poly-Ala
    Compositional biasi428 – 4347Poly-Ala

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG297494.
    GeneTreeiENSGT00390000008860.
    InParanoidiQ05201.
    KOiK15616.
    OMAiYNSMQQY.
    OrthoDBiEOG7DNNTZ.
    PhylomeDBiQ05201.

    Family and domain databases

    InterProiIPR006545. EYA_dom.
    IPR028472. EYA_fam.
    [Graphical view]
    PANTHERiPTHR10190. PTHR10190. 1 hit.
    TIGRFAMsiTIGR01658. EYA-cons_domain. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q05201-1) [UniParc]FASTAAdd to Basket

    Also known as: I, A

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVTLMPYNYA APRCGLIDKM IEPKVKRPKT DHTDTHERNR LCNLSQQQQQ    50
    QQPQQQQTHQ QQQQQQQQSH QQSHSSTVLA SNGPSSAGAG MGVGVGGGGG 100
    SGGGVGGGVG QCSPLGLPPQ SQPLQPTIGS LASLSGHYSN GNANPNVNSS 150
    SCSLATASSF AQSAGSSFST YQQAGGTSGG VSGEDGVVGG ATVMSHWTHD 200
    GTGSSAAVKS ESRSPGQVHA SLDNGSVAGS NLYGCSSASN PLDGGAVAVN 250
    SSAVAAAAAA VYDGKHDYYY YNSMQQYTPP PFYSGYGTPY AAATAARQAK 300
    MEPGAAAAAA AYLTPSYAAS GNNNSQLYSS PYAGYNNFGQ QDYGGYYNEQ 350
    YGNYYSPANY SPYAVSSPSS SASHGHGFHV AASSNLSESP TDTHSTTPVH 400
    QTTHSPHSPL PISPSTGSGI GPLGNVSAAA AAAALNSSGG SSVGTAGSGG 450
    VATSKTTPTG KTGRARGRRH QQPSPTRSTA SDTGNSEAVK PPERVFVWDL 500
    DETLIIFHTL LSGSYANRYT KDHSSLMTIA FRMEEMVFNM ADTHFFFNEI 550
    EECDQVHIDD VSSDDNGQDL SAYNFATDGF HTNTPPGAPP NLCLPTGVRG 600
    GVDWMRKLAF RYRKIKDIYN SYRGNVGTLL GPGKREAWLQ IRSEIEVATD 650
    NWATLALKCL SMISQRENCV NVLVTSTQLA PALAKVLLFG LGGIFNIENI 700
    YSAHKIGHET CYERIVTRFG RKSTYVVIGD GNEEETAAKA MNFPFWRISA 750
    HSDIRALYTA LDMGFL 766
    Length:766
    Mass (Da):80,657
    Last modified:June 1, 1994 - v1
    Checksum:i3581C26AB1811E74
    GO
    Isoform 2 (identifier: Q05201-2) [UniParc]FASTAAdd to Basket

    Also known as: II, B

    The sequence of this isoform differs from the canonical sequence as follows:
         1-23: MVTLMPYNYAAPRCGLIDKMIEP → MLYNVPCYQNFSTLDYY

    Show »
    Length:760
    Mass (Da):80,164
    Checksum:i7BCC45472692B844
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2323MVTLM…KMIEP → MLYNVPCYQNFSTLDYY in isoform 2. 2 PublicationsVSP_001500Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08501 mRNA. Translation: AAA28723.1.
    L08502 mRNA. Translation: AAA28310.1.
    AE014134 Genomic DNA. Translation: AAF52400.1.
    AE014134 Genomic DNA. Translation: AAN10587.1.
    AY047539 mRNA. Translation: AAK77271.1.
    PIRiA45174.
    B45174.
    RefSeqiNP_523492.1. NM_078768.3. [Q05201-2]
    NP_723188.1. NM_164693.2. [Q05201-1]
    UniGeneiDm.5293.

    Genome annotation databases

    EnsemblMetazoaiFBtr0079335; FBpp0078964; FBgn0000320. [Q05201-1]
    GeneIDi33916.
    KEGGidme:Dmel_CG9554.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08501 mRNA. Translation: AAA28723.1 .
    L08502 mRNA. Translation: AAA28310.1 .
    AE014134 Genomic DNA. Translation: AAF52400.1 .
    AE014134 Genomic DNA. Translation: AAN10587.1 .
    AY047539 mRNA. Translation: AAK77271.1 .
    PIRi A45174.
    B45174.
    RefSeqi NP_523492.1. NM_078768.3. [Q05201-2 ]
    NP_723188.1. NM_164693.2. [Q05201-1 ]
    UniGenei Dm.5293.

    3D structure databases

    ProteinModelPortali Q05201.
    SMRi Q05201. Positions 493-766.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 60071. 18 interactions.
    DIPi DIP-19253N.
    IntActi Q05201. 2 interactions.

    Proteomic databases

    PRIDEi Q05201.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0079335 ; FBpp0078964 ; FBgn0000320 . [Q05201-1 ]
    GeneIDi 33916.
    KEGGi dme:Dmel_CG9554.

    Organism-specific databases

    CTDi 33916.
    FlyBasei FBgn0000320. eya.

    Phylogenomic databases

    eggNOGi NOG297494.
    GeneTreei ENSGT00390000008860.
    InParanoidi Q05201.
    KOi K15616.
    OMAi YNSMQQY.
    OrthoDBi EOG7DNNTZ.
    PhylomeDBi Q05201.

    Miscellaneous databases

    ChiTaRSi eya. drosophila.
    GenomeRNAii 33916.
    NextBioi 785901.

    Gene expression databases

    Bgeei Q05201.

    Family and domain databases

    InterProi IPR006545. EYA_dom.
    IPR028472. EYA_fam.
    [Graphical view ]
    PANTHERi PTHR10190. PTHR10190. 1 hit.
    TIGRFAMsi TIGR01658. EYA-cons_domain. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The eyes absent gene: genetic control of cell survival and differentiation in the developing Drosophila eye."
      Bonini N.M., Leiserson W.M., Benzer S.
      Cell 72:379-395(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Tissue: Head.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: Berkeley.
      Tissue: Head.
    5. "The eye-specification proteins So and Eya form a complex and regulate multiple steps in Drosophila eye development."
      Pignoni F., Hu B., Zavitz K.H., Xiao J., Garrity P.A., Zipursky S.L.
      Cell 91:881-891(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SO.
    6. "Dachshund and eyes absent proteins form a complex and function synergistically to induce ectopic eye development in Drosophila."
      Chen R., Amoui M., Zhang Z., Mardon G.
      Cell 91:893-903(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DAC.
    7. Cited for: CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-499.
    8. "The transcription factor Eyes absent is a protein tyrosine phosphatase."
      Tootle T.L., Silver S.J., Davies E.L., Newman V., Latek R.R., Mills I.A., Selengut J.D., Parlikar B.E., Rebay I.
      Nature 426:299-302(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 493-766, MUTAGENESIS OF ASP-499; SER-676; LYS-705; ASP-730 AND GLU-734.

    Entry informationi

    Entry nameiEYA_DROME
    AccessioniPrimary (citable) accession number: Q05201
    Secondary accession number(s): Q961V4, Q9VMC1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3