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Q05201

- EYA_DROME

UniProt

Q05201 - EYA_DROME

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Protein

Developmental protein eyes absent

Gene

eya

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Thought to play a role in transcription regulation during organogenesis through its intrinsic protein phosphatase activity. The phosphatase activity was shown in vitro. Appears to function together with So and Dac in eye development. Required for the survival of eye progenitor cells at a critical stage in morphogenesis.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 Publication

Cofactori

Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei499 – 4991NucleophileBy similarity
Metal bindingi499 – 4991MagnesiumBy similarity
Active sitei501 – 5011Proton donorBy similarity
Metal bindingi501 – 5011MagnesiumBy similarity
Metal bindingi730 – 7301MagnesiumBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: FlyBase
  3. protein tyrosine phosphatase activity Source: FlyBase
  4. protein tyrosine phosphatase activity, metal-dependent Source: FlyBase
  5. transcription factor binding Source: FlyBase

GO - Biological processi

  1. axon guidance Source: FlyBase
  2. Bolwig's organ morphogenesis Source: FlyBase
  3. compound eye development Source: FlyBase
  4. compound eye photoreceptor development Source: FlyBase
  5. eye-antennal disc morphogenesis Source: UniProtKB
  6. gonad development Source: FlyBase
  7. innate immune response Source: FlyBase
  8. larval somatic muscle development Source: FlyBase
  9. male gonad development Source: FlyBase
  10. mesoderm development Source: FlyBase
  11. negative regulation of cell fate specification Source: FlyBase
  12. optic lobe placode formation Source: FlyBase
  13. ovarian follicle cell development Source: FlyBase
  14. peptidyl-tyrosine dephosphorylation Source: GOC
  15. photoreceptor cell axon guidance Source: FlyBase
  16. pole cell migration Source: FlyBase
  17. positive regulation of gene expression Source: FlyBase
  18. positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  19. protein dephosphorylation Source: FlyBase
  20. response to light stimulus Source: FlyBase
  21. salivary gland morphogenesis Source: FlyBase
  22. spermatogenesis Source: FlyBase
  23. transcription, DNA-templated Source: FlyBase
  24. ventral cord development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Hydrolase, Protein phosphatase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Developmental protein eyes absent (EC:3.1.3.48)
Alternative name(s):
Protein Clift
Gene namesi
Name:eya
Synonyms:cli
ORF Names:CG9554
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000320. eya.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: FlyBase
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi499 – 4991D → N: Highly reduced ectopic eye induction and diminishes degree of ommatidial restoration in eyeless phenotype rescue assay. 2 Publications
Mutagenesisi676 – 6761S → A: Highly reduced ectopic eye induction and diminishes degree of ommatidial restoration in eyeless phenotype rescue assay. 1 Publication
Mutagenesisi705 – 7051K → Q: Highly reduced ectopic eye induction and diminishes degree of ommatidial restoration in eyeless phenotype rescue assay. 1 Publication
Mutagenesisi730 – 7301D → N: Highly reduced ectopic eye induction. 1 Publication
Mutagenesisi734 – 7341E → Q: Highly reduced ectopic eye induction. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 766766Developmental protein eyes absentPRO_0000218655Add
BLAST

Proteomic databases

PRIDEiQ05201.

Expressioni

Gene expression databases

BgeeiQ05201.

Interactioni

Subunit structurei

Interacts with Dac and So.2 Publications

Protein-protein interaction databases

BioGridi60071. 18 interactions.
DIPiDIP-19253N.
IntActiQ05201. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ05201.
SMRiQ05201. Positions 493-766.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi46 – 5712Poly-GlnAdd
BLAST
Compositional biasi60 – 689Poly-Gln
Compositional biasi92 – 10817Poly-GlyAdd
BLAST
Compositional biasi253 – 2608Poly-Ala
Compositional biasi268 – 2714Poly-Tyr
Compositional biasi305 – 3117Poly-Ala
Compositional biasi428 – 4347Poly-Ala

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG297494.
GeneTreeiENSGT00390000008860.
InParanoidiQ05201.
KOiK15616.
OMAiYNSMQQY.
OrthoDBiEOG7DNNTZ.
PhylomeDBiQ05201.

Family and domain databases

InterProiIPR006545. EYA_dom.
IPR028472. EYA_fam.
[Graphical view]
PANTHERiPTHR10190. PTHR10190. 1 hit.
TIGRFAMsiTIGR01658. EYA-cons_domain. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q05201-1) [UniParc]FASTAAdd to Basket

Also known as: I, A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVTLMPYNYA APRCGLIDKM IEPKVKRPKT DHTDTHERNR LCNLSQQQQQ
60 70 80 90 100
QQPQQQQTHQ QQQQQQQQSH QQSHSSTVLA SNGPSSAGAG MGVGVGGGGG
110 120 130 140 150
SGGGVGGGVG QCSPLGLPPQ SQPLQPTIGS LASLSGHYSN GNANPNVNSS
160 170 180 190 200
SCSLATASSF AQSAGSSFST YQQAGGTSGG VSGEDGVVGG ATVMSHWTHD
210 220 230 240 250
GTGSSAAVKS ESRSPGQVHA SLDNGSVAGS NLYGCSSASN PLDGGAVAVN
260 270 280 290 300
SSAVAAAAAA VYDGKHDYYY YNSMQQYTPP PFYSGYGTPY AAATAARQAK
310 320 330 340 350
MEPGAAAAAA AYLTPSYAAS GNNNSQLYSS PYAGYNNFGQ QDYGGYYNEQ
360 370 380 390 400
YGNYYSPANY SPYAVSSPSS SASHGHGFHV AASSNLSESP TDTHSTTPVH
410 420 430 440 450
QTTHSPHSPL PISPSTGSGI GPLGNVSAAA AAAALNSSGG SSVGTAGSGG
460 470 480 490 500
VATSKTTPTG KTGRARGRRH QQPSPTRSTA SDTGNSEAVK PPERVFVWDL
510 520 530 540 550
DETLIIFHTL LSGSYANRYT KDHSSLMTIA FRMEEMVFNM ADTHFFFNEI
560 570 580 590 600
EECDQVHIDD VSSDDNGQDL SAYNFATDGF HTNTPPGAPP NLCLPTGVRG
610 620 630 640 650
GVDWMRKLAF RYRKIKDIYN SYRGNVGTLL GPGKREAWLQ IRSEIEVATD
660 670 680 690 700
NWATLALKCL SMISQRENCV NVLVTSTQLA PALAKVLLFG LGGIFNIENI
710 720 730 740 750
YSAHKIGHET CYERIVTRFG RKSTYVVIGD GNEEETAAKA MNFPFWRISA
760
HSDIRALYTA LDMGFL
Length:766
Mass (Da):80,657
Last modified:June 1, 1994 - v1
Checksum:i3581C26AB1811E74
GO
Isoform 2 (identifier: Q05201-2) [UniParc]FASTAAdd to Basket

Also known as: II, B

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: MVTLMPYNYAAPRCGLIDKMIEP → MLYNVPCYQNFSTLDYY

Show »
Length:760
Mass (Da):80,164
Checksum:i7BCC45472692B844
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2323MVTLM…KMIEP → MLYNVPCYQNFSTLDYY in isoform 2. 2 PublicationsVSP_001500Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L08501 mRNA. Translation: AAA28723.1.
L08502 mRNA. Translation: AAA28310.1.
AE014134 Genomic DNA. Translation: AAF52400.1.
AE014134 Genomic DNA. Translation: AAN10587.1.
AY047539 mRNA. Translation: AAK77271.1.
PIRiA45174.
B45174.
RefSeqiNP_523492.1. NM_078768.4. [Q05201-2]
NP_723188.1. NM_164693.2. [Q05201-1]
UniGeneiDm.5293.

Genome annotation databases

EnsemblMetazoaiFBtr0079335; FBpp0078964; FBgn0000320. [Q05201-1]
GeneIDi33916.
KEGGidme:Dmel_CG9554.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L08501 mRNA. Translation: AAA28723.1 .
L08502 mRNA. Translation: AAA28310.1 .
AE014134 Genomic DNA. Translation: AAF52400.1 .
AE014134 Genomic DNA. Translation: AAN10587.1 .
AY047539 mRNA. Translation: AAK77271.1 .
PIRi A45174.
B45174.
RefSeqi NP_523492.1. NM_078768.4. [Q05201-2 ]
NP_723188.1. NM_164693.2. [Q05201-1 ]
UniGenei Dm.5293.

3D structure databases

ProteinModelPortali Q05201.
SMRi Q05201. Positions 493-766.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 60071. 18 interactions.
DIPi DIP-19253N.
IntActi Q05201. 2 interactions.

Proteomic databases

PRIDEi Q05201.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0079335 ; FBpp0078964 ; FBgn0000320 . [Q05201-1 ]
GeneIDi 33916.
KEGGi dme:Dmel_CG9554.

Organism-specific databases

CTDi 33916.
FlyBasei FBgn0000320. eya.

Phylogenomic databases

eggNOGi NOG297494.
GeneTreei ENSGT00390000008860.
InParanoidi Q05201.
KOi K15616.
OMAi YNSMQQY.
OrthoDBi EOG7DNNTZ.
PhylomeDBi Q05201.

Miscellaneous databases

ChiTaRSi eya. drosophila.
GenomeRNAii 33916.
NextBioi 785901.

Gene expression databases

Bgeei Q05201.

Family and domain databases

InterProi IPR006545. EYA_dom.
IPR028472. EYA_fam.
[Graphical view ]
PANTHERi PTHR10190. PTHR10190. 1 hit.
TIGRFAMsi TIGR01658. EYA-cons_domain. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The eyes absent gene: genetic control of cell survival and differentiation in the developing Drosophila eye."
    Bonini N.M., Leiserson W.M., Benzer S.
    Cell 72:379-395(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Berkeley.
    Tissue: Head.
  5. "The eye-specification proteins So and Eya form a complex and regulate multiple steps in Drosophila eye development."
    Pignoni F., Hu B., Zavitz K.H., Xiao J., Garrity P.A., Zipursky S.L.
    Cell 91:881-891(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SO.
  6. "Dachshund and eyes absent proteins form a complex and function synergistically to induce ectopic eye development in Drosophila."
    Chen R., Amoui M., Zhang Z., Mardon G.
    Cell 91:893-903(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DAC.
  7. Cited for: CATALYTIC ACTIVITY, MUTAGENESIS OF ASP-499.
  8. "The transcription factor Eyes absent is a protein tyrosine phosphatase."
    Tootle T.L., Silver S.J., Davies E.L., Newman V., Latek R.R., Mills I.A., Selengut J.D., Parlikar B.E., Rebay I.
    Nature 426:299-302(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 493-766, MUTAGENESIS OF ASP-499; SER-676; LYS-705; ASP-730 AND GLU-734.

Entry informationi

Entry nameiEYA_DROME
AccessioniPrimary (citable) accession number: Q05201
Secondary accession number(s): Q961V4, Q9VMC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3