Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q05199 (NRG1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pro-neuregulin-1, membrane-bound isoform

Short name=Pro-NRG1

Cleaved into the following chain:

  1. Neuregulin-1
    Alternative name(s):
    Acetylcholine receptor-inducing activity
    Short name=ARIA
Gene names
Name:NRG1
Synonyms:ARIA
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Direct ligand for the ERBB tyrosine kinase receptors. The multiple isoforms perform diverse functions: cysteine-rich domain containing isoforms (isoform 2-isoform4) probably regulate the expression of nicotinic acetylcholine receptors at developing interneuronal synapses. Isoform Ig-NRGis required for the initial induction and/or maintenance of the mature levels of acetylcholine receptors at neuromuscular synapses.

Subcellular location

Pro-neuregulin-1, membrane-bound isoform: Cell membrane; Single-pass type I membrane protein By similarity. Note: Does not seem to be active By similarity.

Neuregulin-1: Secreted By similarity.

Developmental stage

Isoform 2-isoform4 are detected at embryonic day 4 (ED4) in both visceral and somatic motor neurons of spinal cord and is highest at ED6. Isoform 1 is not expressed until ED 6 in spinal cord. At ED 11 both isoforms display comparable levels.

Domain

The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization By similarity.

ERBB receptor binding is elicited entirely by the EGF-like domain.

Post-translational modification

Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.

Extensive glycosylation precedes the proteolytic cleavage By similarity.

Sequence similarities

Belongs to the neuregulin family.

Contains 1 EGF-like domain.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q05199-1)

Also known as: ARIA; Ig-NRG;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Contains an Ig-like domain.
Isoform 2 (identifier: Q05199-2)

Also known as: CRD-NRG-BETA1A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-127: MWATSEGPLQ...ASVIITDTNA → MSEVGTETFP...TNLQTAPKLS
Note: The EGF-like domain is replaced by a cysteine-rich domain (CRD).
Isoform 3 (identifier: Q05199-3)

Also known as: CRD-NRG-BETA2A;

The sequence of this isoform differs from the canonical sequence as follows:
     1-127: MWATSEGPLQ...ASVIITDTNA → MSEVGTETFP...TNLQTAPKLS
     191-198: Missing.
Note: The EGF-like domain is replaced by a cysteine-rich domain (CRD).
Isoform 4 (identifier: Q05199-4)

Also known as: CRD-NRG-BETA2B;

The sequence of this isoform differs from the canonical sequence as follows:
     1-127: MWATSEGPLQ...ASVIITDTNA → MSEVGTETFP...TNLQTAPKLS
     191-198: Missing.
     388-405: VSAMTTPARMSPVDFHTP → HTPPTSLLLAGKVSLRVS
     406-602: Missing.
Note: The EGF-like domain is replaced by a cysteine-rich domain (CRD).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 602602Pro-neuregulin-1, membrane-bound isoform
PRO_0000019468
Chain1 – 205205Neuregulin-1
PRO_0000019469

Regions

Topological domain1 – 206206Extracellular Potential
Transmembrane207 – 22923Helical; Note=Internal signal sequence; Potential
Topological domain230 – 602373Cytoplasmic Potential
Domain29 – 12395Ig-like C2-type
Domain137 – 18145EGF-like
Compositional bias125 – 13612Ser/Thr-rich

Amino acid modifications

Glycosylation211N-linked (GlcNAc...) Potential
Glycosylation1131N-linked (GlcNAc...) Potential
Glycosylation1261N-linked (GlcNAc...) Potential
Disulfide bond49 ↔ 105 By similarity
Disulfide bond141 ↔ 155 By similarity
Disulfide bond149 ↔ 169 By similarity
Disulfide bond171 ↔ 180 By similarity

Natural variations

Alternative sequence1 – 127127MWATS…TDTNA → MSEVGTETFPSPSAQLSPDA SLGGLPAEENMPGPHREDSR VPGVAGLASTCCVCLEAERL KGCLNSEKICIAPILACLLS LCLCIAGLKWVFVDKIFEYD SPTHLDPGRIGQDPRSTVDP TALSAWVPSEVYASPFPIPS LESKAEVTVQTDSSLVPSRP FLQPSLYNRILDVGLWSSAT PSLSPSSLEPTTASQAQATE TNLQTAPKLS in isoform 2, isoform 3 and isoform 4.
VSP_003445
Alternative sequence191 – 1988Missing in isoform 3 and isoform 4.
VSP_003446
Alternative sequence388 – 40518VSAMT…DFHTP → HTPPTSLLLAGKVSLRVS in isoform 4.
VSP_003447
Alternative sequence406 – 602197Missing in isoform 4.
VSP_003448

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (ARIA) (Ig-NRG) [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4183C0E56CE5D346

FASTA60267,454
        10         20         30         40         50         60 
MWATSEGPLQ YSLAPTQTDV NSSYNTVPPK LKEMKNQEVA VGQKLVLRCE TTSEYPALRF 

        70         80         90        100        110        120 
KWLKNGKEIT KKNRPENVKI PKKQKKYSEL HIYRATLADA GEYACRVSSK LGNDSTKASV 

       130        140        150        160        170        180 
IITDTNATST STTGTSHLTK CDIKQKAFCV NGGECYMVKD LPNPPRYLCR CPNEFTGDRC 

       190        200        210        220        230        240 
QNYVMASFYK HLGIEFMEAE ELYQKRVLTI TGICIALLVV GIMCVVAYCK TKKQRKKLHD 

       250        260        270        280        290        300 
RLRQSLRSER NNVMNMANGP HHPNPPPDNV QLVNQYVSKN IISSERVVER ETETSFSTSH 

       310        320        330        340        350        360 
YTSTTHHSMT VTQTPSHSWS NGHTESILSE SHSVLVSSSV ENSRHTSPTG PRGRLNGIGG 

       370        380        390        400        410        420 
PREGNSFLRH ARETPDSYRD SPHSERYVSA MTTPARMSPV DFHTPTSPKS PPSEMSPPVS 

       430        440        450        460        470        480 
SLTISIPSVA VSPFMDEERP LLLVTPPRLR EKYDNHLQQF NSFHNNPTHE SNSLPPSPLR 

       490        500        510        520        530        540 
IVEDEEYETT QEYEPAQEPP KKLTNSRRVK RTKPNGHISS RVEVDSDTSS QSTSSESETE 

       550        560        570        580        590        600 
DERIGEDTPF LSIQNPMATS LEPAAAYRLA ENRTNPANRF STPEELQARL SSVIANQDPI 


AV 

« Hide

Isoform 2 (CRD-NRG-BETA1A) [UniParc].

Checksum: B2634881D40B3398
Show »

FASTA68575,415
Isoform 3 (CRD-NRG-BETA2A) [UniParc].

Checksum: D6C0F3C45B64E0EC
Show »

FASTA67774,457
Isoform 4 (CRD-NRG-BETA2B) [UniParc].

Checksum: 9B95A898A4669A93
Show »

FASTA48052,440

References

[1]"ARIA, a protein that stimulates acetylcholine receptor synthesis, is a member of the neu ligand family."
Falls D.L., Rosen K.M., Corfas G., Lane W.S., Fischbach G.D.
Cell 72:801-815(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
Strain: White leghorn.
Tissue: Brain.
[2]"A cysteine-rich isoform of neuregulin controls the level of expression of neuronal nicotinic receptor channels during synaptogenesis."
Yang X., Kuo Y., Devay P., Yu C., Role L.
Neuron 20:255-270(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4).
Tissue: Brain and Spinal cord.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L11264 mRNA. Translation: AAA49037.1.
AF045654 mRNA. Translation: AAC05670.1.
AF045655 mRNA. Translation: AAC05671.1.
AF045656 mRNA. Translation: AAC05672.1.
PIRA45769.
RefSeqNP_989448.1. NM_204117.1. [Q05199-2]
UniGeneGga.135.

3D structure databases

ProteinModelPortalQ05199.
SMRQ05199. Positions 136-203.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ05199.
PRIDEQ05199.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSGALT00000037781; ENSGALP00000036986; ENSGALG00000015422. [Q05199-1]
GeneID373906.
KEGGgga:373906.

Organism-specific databases

CTD3084.

Phylogenomic databases

eggNOGNOG47801.
GeneTreeENSGT00710000106660.
HOGENOMHOG000273863.
HOVERGENHBG006531.
KOK05455.
PhylomeDBQ05199.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003598. Ig_sub2.
IPR018250. Neuregulin.
IPR002154. Neuregulin_1_C.
[Graphical view]
PfamPF07679. I-set. 1 hit.
PF02158. Neuregulin. 1 hit.
[Graphical view]
PRINTSPR01089. NEUREGULIN.
SMARTSM00181. EGF. 1 hit.
SM00408. IGc2. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 1 hit.
PS50026. EGF_3. 1 hit.
PS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20813438.
PROQ05199.

Entry information

Entry nameNRG1_CHICK
AccessionPrimary (citable) accession number: Q05199
Secondary accession number(s): O73750, O73751, O73752
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families