ID   TGMH_TACTR              Reviewed;         764 AA.
AC   Q05187;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 61.
DE   RecName: Full=Hemocyte protein-glutamine gamma-glutamyltransferase;
DE            EC=2.3.2.13;
DE   AltName: Full=Hemocyte transglutaminase;
DE            Short=TGase;
OS   Tachypleus tridentatus (Japanese horseshoe crab).
OC   Eukaryota; Metazoa; Arthropoda; Chelicerata; Merostomata; Xiphosura;
OC   Limulidae; Tachypleus.
OX   NCBI_TaxID=6853;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Hemocyte;
RX   MEDLINE=93107012; PubMed=8093243;
RA   Tokunaga F., Muta T., Iwanaga S., Ichinose A., Davie E.W., Kuma K.,
RA   Miyata T.;
RT   "Limulus hemocyte transglutaminase. cDNA cloning, amino acid sequence,
RT   and tissue localization.";
RL   J. Biol. Chem. 268:262-268(1993).
CC   -!- FUNCTION: Catalyzes the cross-linking of proteins and the
CC       conjugation of polyamines to proteins.
CC   -!- CATALYTIC ACTIVITY: Protein glutamine + alkylamine = protein N(5)-
CC       alkylglutamine + NH(3).
CC   -!- COFACTOR: Binds 1 calcium ion per subunit (By similarity).
CC   -!- SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
CC   -!- TISSUE SPECIFICITY: Mainly expressed in hemocytes, hepatopancreas,
CC       and gastric tissues. On the other hand nothing was detected in the
CC       heart, intestine and muscle.
CC   -!- SIMILARITY: Belongs to the transglutaminase superfamily.
CC       Transglutaminase family.
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DR   EMBL; D12593; BAA02134.1; -; mRNA.
DR   PIR; A45321; A45321.
DR   HSSP; P00488; 1EVU.
DR   BRENDA; 2.3.2.13; 18641.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008415; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0005509; F:calcium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003810; F:protein-glutamine gamma-glutamyltransferase...; IEA:EC.
DR   GO; GO:0018149; P:peptide cross-linking; IEA:InterPro.
DR   InterPro; IPR008957; Fibronectin_typ-III-like_fold.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR002931; Transglutaminase-like.
DR   InterPro; IPR008958; Transglutaminase_C.
DR   InterPro; IPR013808; Transglutaminase_CS.
DR   InterPro; IPR001102; Transglutaminase_N.
DR   Gene3D; G3DSA:2.60.40.30; FN_III-like; 1.
DR   Gene3D; G3DSA:2.60.40.10; Ig-like_fold; 1.
DR   Pfam; PF00927; Transglut_C; 2.
DR   Pfam; PF01841; Transglut_core; 1.
DR   Pfam; PF00868; Transglut_N; 1.
DR   SMART; SM00460; TGc; 1.
DR   PROSITE; PS00547; TRANSGLUTAMINASES; 1.
PE   1: Evidence at protein level;
KW   Acyltransferase; Calcium; Direct protein sequencing; Membrane;
KW   Metal-binding; Transferase.
FT   CHAIN         1    764       Hemocyte protein-glutamine gamma-
FT                                glutamyltransferase.
FT                                /FTId=PRO_0000213717.
FT   ACT_SITE    343    343       By similarity.
FT   ACT_SITE    402    402       By similarity.
FT   ACT_SITE    425    425       By similarity.
FT   METAL       465    465       Calcium (By similarity).
FT   METAL       467    467       Calcium (By similarity).
FT   METAL       522    522       Calcium (By similarity).
FT   METAL       527    527       Calcium (By similarity).
FT   VARIANT     452    452       G -> R.
FT   VARIANT     477    477       S -> C.
FT   VARIANT     486    486       I -> S.
SQ   SEQUENCE   764 AA;  86996 MW;  D4F1D392F1DA5811 CRC64;
     MYGFGRGNMF RNRSTRYRRR PRYRAENYHS YMLDLLENMN EEFGRNWWGT PESHQPDSGP
     SSLQVESVEL YTRDNAREHN TFMYDLVDGT KPVLILRRGQ PFSIAIRFKR NYNPQQDRLK
     LEIGFGQQPL ITKGTLIMLP VSGSDTFTKD KTQWDVRLRQ HDGAVITLEI QIPAAVAVGV
     WKMKIVSQLT SEEQPNVSAV THECKNKTYI LFNPWCKQDS VYMEDEQWRK EYVLSDVGKI
     FTGSFKQPVG RRWIFGQFTD SVLPACMLIL ERSGLDYTAR SNPIKVVRAI SAMVNNIDDE
     GVLEGRWQEP YDDGVAPWMW TGSSAILEKY LKTRGVPVKY GQCWVFAGVA NTVSRALGIP
     SRTVTNYDSA HDTDDTLTID KWFDKNGDKI EDATSDSIWN FHVWNDCWMA RPDLPTGYGG
     WQAYDSTPQE TSEGVYQTGP ASVLAVQRGE IGYMFDSPFV FSEVNADVVH WQEDDSSETG
     YKKLKIDSYR VGRLLLTKKI GVDDDFGDAD AEDITDQYKN KEGTDEERMS VLNAARSSGF
     NYAFNLPSPE KEDVYFNLLD IEKIKIGQPF HVTVNIENQS SETRRVSAVL SASSIYYTGI
     TGRKIKRENG NFSLQPHQKE VLSIEVTPDE YLEKLVDYAM IKLYAIATVK ETQQTWSEED
     DFMVEKPNLE LEIRGNLQVG TAFVLAISLT NPLKRVLDNC FFTIEAPGVT GAFRVTNRDI
     QPEEVAVHTV RLIPQKPGPR KIVATFSSRQ LIQVVGSKQV EVLD
//