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Q05187 (TGMH_TACTR) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hemocyte protein-glutamine gamma-glutamyltransferase

EC=2.3.2.13
Alternative name(s):
Hemocyte transglutaminase
Short name=TGase
OrganismTachypleus tridentatus (Japanese horseshoe crab)
Taxonomic identifier6853 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataMerostomataXiphosuraLimulidaeTachypleus

Protein attributes

Sequence length764 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.

Catalytic activity

Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subcellular location

Membrane; Peripheral membrane protein.

Tissue specificity

Mainly expressed in hemocytes, hepatopancreas, and gastric tissues. On the other hand nothing was detected in the heart, intestine and muscle.

Sequence similarities

Belongs to the transglutaminase superfamily. Transglutaminase family.

Ontologies

Keywords
   Cellular componentMembrane
   LigandCalcium
Metal-binding
   Molecular functionAcyltransferase
Transferase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processpeptide cross-linking

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmembrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein-glutamine gamma-glutamyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 764764Hemocyte protein-glutamine gamma-glutamyltransferase
PRO_0000213717

Sites

Active site3431 By similarity
Active site4021 By similarity
Active site4251 By similarity
Metal binding4651Calcium By similarity
Metal binding4671Calcium By similarity
Metal binding5221Calcium By similarity
Metal binding5271Calcium By similarity

Natural variations

Natural variant4521G → R.
Natural variant4771S → C.
Natural variant4861I → S.

Sequences

Sequence LengthMass (Da)Tools
Q05187 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: D4F1D392F1DA5811

FASTA76486,996
        10         20         30         40         50         60 
MYGFGRGNMF RNRSTRYRRR PRYRAENYHS YMLDLLENMN EEFGRNWWGT PESHQPDSGP 

        70         80         90        100        110        120 
SSLQVESVEL YTRDNAREHN TFMYDLVDGT KPVLILRRGQ PFSIAIRFKR NYNPQQDRLK 

       130        140        150        160        170        180 
LEIGFGQQPL ITKGTLIMLP VSGSDTFTKD KTQWDVRLRQ HDGAVITLEI QIPAAVAVGV 

       190        200        210        220        230        240 
WKMKIVSQLT SEEQPNVSAV THECKNKTYI LFNPWCKQDS VYMEDEQWRK EYVLSDVGKI 

       250        260        270        280        290        300 
FTGSFKQPVG RRWIFGQFTD SVLPACMLIL ERSGLDYTAR SNPIKVVRAI SAMVNNIDDE 

       310        320        330        340        350        360 
GVLEGRWQEP YDDGVAPWMW TGSSAILEKY LKTRGVPVKY GQCWVFAGVA NTVSRALGIP 

       370        380        390        400        410        420 
SRTVTNYDSA HDTDDTLTID KWFDKNGDKI EDATSDSIWN FHVWNDCWMA RPDLPTGYGG 

       430        440        450        460        470        480 
WQAYDSTPQE TSEGVYQTGP ASVLAVQRGE IGYMFDSPFV FSEVNADVVH WQEDDSSETG 

       490        500        510        520        530        540 
YKKLKIDSYR VGRLLLTKKI GVDDDFGDAD AEDITDQYKN KEGTDEERMS VLNAARSSGF 

       550        560        570        580        590        600 
NYAFNLPSPE KEDVYFNLLD IEKIKIGQPF HVTVNIENQS SETRRVSAVL SASSIYYTGI 

       610        620        630        640        650        660 
TGRKIKRENG NFSLQPHQKE VLSIEVTPDE YLEKLVDYAM IKLYAIATVK ETQQTWSEED 

       670        680        690        700        710        720 
DFMVEKPNLE LEIRGNLQVG TAFVLAISLT NPLKRVLDNC FFTIEAPGVT GAFRVTNRDI 

       730        740        750        760 
QPEEVAVHTV RLIPQKPGPR KIVATFSSRQ LIQVVGSKQV EVLD 

« Hide

References

[1]"Limulus hemocyte transglutaminase. cDNA cloning, amino acid sequence, and tissue localization."
Tokunaga F., Muta T., Iwanaga S., Ichinose A., Davie E.W., Kuma K., Miyata T.
J. Biol. Chem. 268:262-268(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Hemocyte.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D12593 mRNA. Translation: BAA02134.1.
PIRA45321.

3D structure databases

ProteinModelPortalQ05187.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.10. 3 hits.
3.90.260.10. 1 hit.
InterProIPR023608. Gln_gamma-glutamylTfrase_euk.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002931. Transglutaminase-like.
IPR008958. Transglutaminase_C.
IPR013808. Transglutaminase_CS.
IPR001102. Transglutaminase_N.
[Graphical view]
PANTHERPTHR11590. PTHR11590. 1 hit.
PfamPF00927. Transglut_C. 2 hits.
PF01841. Transglut_core. 1 hit.
PF00868. Transglut_N. 1 hit.
[Graphical view]
PIRSFPIRSF000459. TGM_EBP42. 1 hit.
SMARTSM00460. TGc. 1 hit.
[Graphical view]
SUPFAMSSF49309. SSF49309. 2 hits.
SSF81296. SSF81296. 1 hit.
PROSITEPS00547. TRANSGLUTAMINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTGMH_TACTR
AccessionPrimary (citable) accession number: Q05187
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 16, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families