Reviewed, UniProtKB/Swiss-Prot Q05187 (TGMH_TACTR)

Names and origin

Protein names	Recommended name:     Hemocyte protein-glutamine gamma-glutamyltransferase     EC=2.3.2.13 Alternative name(s):     Hemocyte transglutaminase       Short name=TGase
Organism	Tachypleus tridentatus (Japanese horseshoe crab)
Taxonomic identifier	6853 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Chelicerata › Merostomata › Xiphosura › Limulidae › Tachypleus

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Catalytic activity	Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Membrane; Peripheral membrane protein.
Tissue specificity	Mainly expressed in hemocytes, hepatopancreas, and gastric tissues. On the other hand nothing was detected in the heart, intestine and muscle.
Sequence similarities	Belongs to the transglutaminase superfamily. Transglutaminase family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	Calcium Metal-binding
Molecular function	Acyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	peptide cross-linking Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein-glutamine gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	Hemocyte protein-glutamine gamma-glutamyltransferase		PRO_0000213717
Sites
	Active site	343	1	By similarity
	Active site	402	1	By similarity
	Active site	425	1	By similarity
	Metal binding	465	1	Calcium By similarity
	Metal binding	467	1	Calcium By similarity
	Metal binding	522	1	Calcium By similarity
	Metal binding	527	1	Calcium By similarity
Natural variations
	Natural variant	452	1	G → R
	Natural variant	477	1	S → C
	Natural variant	486	1	I → S

Sequences

	Sequence		Length	Mass (Da)
	Q05187-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D4F1D392F1DA5811	FASTA	764	86,996
	10 20 30 40 50 60 MYGFGRGNMF RNRSTRYRRR PRYRAENYHS YMLDLLENMN EEFGRNWWGT PESHQPDSGP 70 80 90 100 110 120 SSLQVESVEL YTRDNAREHN TFMYDLVDGT KPVLILRRGQ PFSIAIRFKR NYNPQQDRLK 130 140 150 160 170 180 LEIGFGQQPL ITKGTLIMLP VSGSDTFTKD KTQWDVRLRQ HDGAVITLEI QIPAAVAVGV 190 200 210 220 230 240 WKMKIVSQLT SEEQPNVSAV THECKNKTYI LFNPWCKQDS VYMEDEQWRK EYVLSDVGKI 250 260 270 280 290 300 FTGSFKQPVG RRWIFGQFTD SVLPACMLIL ERSGLDYTAR SNPIKVVRAI SAMVNNIDDE 310 320 330 340 350 360 GVLEGRWQEP YDDGVAPWMW TGSSAILEKY LKTRGVPVKY GQCWVFAGVA NTVSRALGIP 370 380 390 400 410 420 SRTVTNYDSA HDTDDTLTID KWFDKNGDKI EDATSDSIWN FHVWNDCWMA RPDLPTGYGG 430 440 450 460 470 480 WQAYDSTPQE TSEGVYQTGP ASVLAVQRGE IGYMFDSPFV FSEVNADVVH WQEDDSSETG 490 500 510 520 530 540 YKKLKIDSYR VGRLLLTKKI GVDDDFGDAD AEDITDQYKN KEGTDEERMS VLNAARSSGF 550 560 570 580 590 600 NYAFNLPSPE KEDVYFNLLD IEKIKIGQPF HVTVNIENQS SETRRVSAVL SASSIYYTGI 610 620 630 640 650 660 TGRKIKRENG NFSLQPHQKE VLSIEVTPDE YLEKLVDYAM IKLYAIATVK ETQQTWSEED 670 680 690 700 710 720 DFMVEKPNLE LEIRGNLQVG TAFVLAISLT NPLKRVLDNC FFTIEAPGVT GAFRVTNRDI 730 740 750 760 QPEEVAVHTV RLIPQKPGPR KIVATFSSRQ LIQVVGSKQV EVLD

References

[1]

"Limulus hemocyte transglutaminase. cDNA cloning, amino acid sequence, and tissue localization." Tokunaga F., Muta T., Iwanaga S., Ichinose A., Davie E.W., Kuma K., Miyata T. J. Biol. Chem. 268:262-268(1993) [PubMed: 8093243] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Hemocyte.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D12593 mRNA. Translation: BAA02134.1.
PIR	A45321.
3D structure databases
HSSP	HSSP built from PDB template 1EVU based on UniProtKB P00488.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.2.13. 18641.
Family and domain databases
InterPro	IPR008957. Fibronectin_typ-III-like_fold. IPR013783. Ig-like_fold. IPR002931. Transglutaminase-like. IPR008958. Transglutaminase_C. IPR013808. Transglutaminase_CS. IPR001102. Transglutaminase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00927. Transglut_C. 2 hits. PF01841. Transglut_core. 1 hit. PF00868. Transglut_N. 1 hit. [Graphical view]
SMART	SM00460. TGc. 1 hit. [Graphical view]
PROSITE	PS00547. TRANSGLUTAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TGMH_TACTR

Accession

Primary (citable) accession number: Q05187

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)