Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q05186

- RCN1_MOUSE

UniProt

Q05186 - RCN1_MOUSE

Protein

Reticulocalbin-1

Gene

Rcn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi86 – 97121Add
    BLAST
    Calcium bindingi122 – 133122; possibly ancestralAdd
    BLAST
    Calcium bindingi173 – 184123Add
    BLAST
    Calcium bindingi210 – 221124Add
    BLAST
    Calcium bindingi251 – 262125Add
    BLAST
    Calcium bindingi287 – 298126; possibly ancestralAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro

    GO - Biological processi

    1. camera-type eye development Source: MGI
    2. in utero embryonic development Source: MGI

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Reticulocalbin-1
    Gene namesi
    Name:Rcn1
    Synonyms:Rca1, Rcn
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:104559. Rcn1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 325302Reticulocalbin-1PRO_0000004146Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi47 – 471N-linked (GlcNAc...); partial

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ05186.
    PaxDbiQ05186.
    PRIDEiQ05186.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00137831.

    PTM databases

    PhosphoSiteiQ05186.

    Expressioni

    Gene expression databases

    BgeeiQ05186.
    CleanExiMM_RCN1.
    GenevestigatoriQ05186.

    Interactioni

    Protein-protein interaction databases

    BioGridi202839. 2 interactions.
    IntActiQ05186. 1 interaction.
    MINTiMINT-4132049.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05186.
    SMRiQ05186. Positions 12-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini73 – 10836EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini109 – 14436EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini160 – 19536EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini197 – 23236EF-hand 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini238 – 27336EF-hand 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini274 – 30936EF-hand 6PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi322 – 3254Prevents secretion from ER

    Sequence similaritiesi

    Belongs to the CREC family.Curated
    Contains 6 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG271367.
    GeneTreeiENSGT00550000074546.
    HOGENOMiHOG000230934.
    HOVERGENiHBG002834.
    InParanoidiQ05186.
    OMAiNPEEFQD.
    OrthoDBiEOG73Z2TD.
    PhylomeDBiQ05186.
    TreeFamiTF314849.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR027241. Rcn1.
    [Graphical view]
    PANTHERiPTHR10827:SF17. PTHR10827:SF17. 1 hit.
    PfamiPF13202. EF-hand_5. 2 hits.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 3 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 3 hits.
    PS50222. EF_HAND_2. 6 hits.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05186-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARGGRLGLA LGLLLALVLA LRAKPTVRKE RVVRPDSELG ERPPEDNQSF    50
    QYDHEAFLGK EDSKTFDQLS PDESKERLGK IVDRIDSDGD GLVTTEELKL 100
    WIKRVQKRYI YDNVAKVWKD YDRDKDEKIS WEEYKQATYG YYLGNPAEFH 150
    DSSDHHTFKK MLPRDERRFK ASDLDGDLTA TREEFTAFLH PEEFEHMKEI 200
    VVLETLEDID KNGDGFVDQD EYIADMFSHE DNGPEPDWVL SEREQFNDFR 250
    DLNKDGKLDK DEIRHWILPQ DYDHAQAEAR HLVYESDKNK DEMLTKEEIL 300
    DNWNMFVGSQ ATNYGEDLTK NHDEL 325
    Length:325
    Mass (Da):38,113
    Last modified:June 1, 1994 - v1
    Checksum:i0470B10B5A8BC76D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241K → G AA sequence (PubMed:7523108)Curated
    Sequence conflicti34 – 341R → I AA sequence (PubMed:7523108)Curated
    Sequence conflicti37 – 393SEL → DEE AA sequence (PubMed:7523108)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13003 mRNA. Translation: BAA02366.1.
    D43956 Genomic DNA. Translation: BAA07896.1.
    BC049108 mRNA. Translation: AAH49108.1.
    AK017494 mRNA. Translation: BAB30773.1.
    AK133971 mRNA. Translation: BAE21962.1.
    AK159973 mRNA. Translation: BAE35525.1.
    CCDSiCCDS16498.1.
    PIRiA45337.
    RefSeqiNP_033063.1. NM_009037.2.
    UniGeneiMm.4876.

    Genome annotation databases

    EnsembliENSMUST00000006128; ENSMUSP00000006128; ENSMUSG00000005973.
    GeneIDi19672.
    KEGGimmu:19672.
    UCSCiuc008lks.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13003 mRNA. Translation: BAA02366.1 .
    D43956 Genomic DNA. Translation: BAA07896.1 .
    BC049108 mRNA. Translation: AAH49108.1 .
    AK017494 mRNA. Translation: BAB30773.1 .
    AK133971 mRNA. Translation: BAE21962.1 .
    AK159973 mRNA. Translation: BAE35525.1 .
    CCDSi CCDS16498.1.
    PIRi A45337.
    RefSeqi NP_033063.1. NM_009037.2.
    UniGenei Mm.4876.

    3D structure databases

    ProteinModelPortali Q05186.
    SMRi Q05186. Positions 12-303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202839. 2 interactions.
    IntActi Q05186. 1 interaction.
    MINTi MINT-4132049.

    PTM databases

    PhosphoSitei Q05186.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00137831.

    Proteomic databases

    MaxQBi Q05186.
    PaxDbi Q05186.
    PRIDEi Q05186.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000006128 ; ENSMUSP00000006128 ; ENSMUSG00000005973 .
    GeneIDi 19672.
    KEGGi mmu:19672.
    UCSCi uc008lks.1. mouse.

    Organism-specific databases

    CTDi 5954.
    MGIi MGI:104559. Rcn1.

    Phylogenomic databases

    eggNOGi NOG271367.
    GeneTreei ENSGT00550000074546.
    HOGENOMi HOG000230934.
    HOVERGENi HBG002834.
    InParanoidi Q05186.
    OMAi NPEEFQD.
    OrthoDBi EOG73Z2TD.
    PhylomeDBi Q05186.
    TreeFami TF314849.

    Miscellaneous databases

    ChiTaRSi RCN1. mouse.
    NextBioi 296990.
    PROi Q05186.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q05186.
    CleanExi MM_RCN1.
    Genevestigatori Q05186.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR027241. Rcn1.
    [Graphical view ]
    PANTHERi PTHR10827:SF17. PTHR10827:SF17. 1 hit.
    Pfami PF13202. EF-hand_5. 2 hits.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 3 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 3 hits.
    PS50222. EF_HAND_2. 6 hits.
    PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Reticulocalbin, a novel endoplasmic reticulum resident Ca(2+)-binding protein with multiple EF-hand motifs and a carboxyl-terminal HDEL sequence."
      Ozawa M., Muramatsu T.
      J. Biol. Chem. 268:699-705(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure of the gene encoding mouse reticulocalbin, a novel endoplasmic reticulum-resident Ca(2+)-binding protein with multiple EF-hand motifs."
      Ozawa M.
      J. Biochem. 118:154-160(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    5. "Separation and sequencing of familiar and novel murine proteins using preparative two-dimensional gel electrophoresis."
      Merrick B.A., Patterson R.M., Wichter L.L., He C., Selkirk J.K.
      Electrophoresis 15:735-745(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-39.
      Tissue: Fibroblast.

    Entry informationi

    Entry nameiRCN1_MOUSE
    AccessioniPrimary (citable) accession number: Q05186
    Secondary accession number(s): Q3TVU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3