Q05186 (RCN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Reticulocalbin-1

Gene names

Name:	Rcn1
Synonyms:	Rca1, Rcn

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.
Subcellular location	Endoplasmic reticulum lumen.
Miscellaneous	This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
Sequence similarities	Belongs to the CREC family. Contains 6 EF-hand domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Calcium Metal-binding
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	camera-type eye development Inferred from mutant phenotype PubMed 19474196. Source: MGI in utero embryonic development Inferred from mutant phenotype PubMed 19474196. Source: MGI
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.5
	Chain	24 – 325	302	Reticulocalbin-1		PRO_0000004146
Regions
	Domain	73 – 108	36	EF-hand 1
	Domain	109 – 144	36	EF-hand 2
	Domain	160 – 195	36	EF-hand 3
	Domain	197 – 232	36	EF-hand 4
	Domain	238 – 273	36	EF-hand 5
	Domain	274 – 309	36	EF-hand 6
	Calcium binding	86 – 97	12	1
	Calcium binding	122 – 133	12	2; possibly ancestral
	Calcium binding	173 – 184	12	3
	Calcium binding	210 – 221	12	4
	Calcium binding	251 – 262	12	5
	Calcium binding	287 – 298	12	6; possibly ancestral
	Motif	322 – 325	4	Prevents secretion from ER
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...); partial
Experimental info
	Sequence conflict	24	1	K → G AA sequence Ref.5
	Sequence conflict	34	1	R → I AA sequence Ref.5
	Sequence conflict	37 – 39	3	SEL → DEE AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q05186 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 0470B10B5A8BC76D	FASTA	325	38,113
	10 20 30 40 50 60 MARGGRLGLA LGLLLALVLA LRAKPTVRKE RVVRPDSELG ERPPEDNQSF QYDHEAFLGK 70 80 90 100 110 120 EDSKTFDQLS PDESKERLGK IVDRIDSDGD GLVTTEELKL WIKRVQKRYI YDNVAKVWKD 130 140 150 160 170 180 YDRDKDEKIS WEEYKQATYG YYLGNPAEFH DSSDHHTFKK MLPRDERRFK ASDLDGDLTA 190 200 210 220 230 240 TREEFTAFLH PEEFEHMKEI VVLETLEDID KNGDGFVDQD EYIADMFSHE DNGPEPDWVL 250 260 270 280 290 300 SEREQFNDFR DLNKDGKLDK DEIRHWILPQ DYDHAQAEAR HLVYESDKNK DEMLTKEEIL 310 320 DNWNMFVGSQ ATNYGEDLTK NHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reticulocalbin, a novel endoplasmic reticulum resident Ca(2+)-binding protein with multiple EF-hand motifs and a carboxyl-terminal HDEL sequence." Ozawa M., Muramatsu T. J. Biol. Chem. 268:699-705(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure of the gene encoding mouse reticulocalbin, a novel endoplasmic reticulum-resident Ca(2+)-binding protein with multiple EF-hand motifs." Ozawa M. J. Biochem. 118:154-160(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[5]	"Separation and sequencing of familiar and novel murine proteins using preparative two-dimensional gel electrophoresis." Merrick B.A., Patterson R.M., Wichter L.L., He C., Selkirk J.K. Electrophoresis 15:735-745(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-39. Tissue: Fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13003 mRNA. Translation: BAA02366.1. D43956 Genomic DNA. Translation: BAA07896.1. BC049108 mRNA. Translation: AAH49108.1. AK017494 mRNA. Translation: BAB30773.1. AK133971 mRNA. Translation: BAE21962.1. AK159973 mRNA. Translation: BAE35525.1.
IPI	IPI00137831.
PIR	A45337.
RefSeq	NP_033063.1. NM_009037.2.
UniGene	Mm.4876.
3D structure databases
ProteinModelPortal	Q05186.
SMR	Q05186. Positions 74-304.
ModBase	Search...
PTM databases
PhosphoSite	Q05186.
2D gel databases
REPRODUCTION-2DPAGE	IPI00137831.
Proteomic databases
PaxDb	Q05186.
PRIDE	Q05186.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000006128; ENSMUSP00000006128; ENSMUSG00000005973.
GeneID	19672.
KEGG	mmu:19672.
Organism-specific databases
CTD	5954.
MGI	MGI:104559. Rcn1.
Phylogenomic databases
eggNOG	NOG271367.
GeneTree	ENSGT00550000074546.
HOGENOM	HOG000230934.
HOVERGEN	HBG002834.
InParanoid	Q05186.
OMA	YIADMFA.
OrthoDB	EOG42RD7R.
Gene expression databases
ArrayExpress	Q05186.
Bgee	Q05186.
CleanEx	MM_RCN1.
Genevestigator	Q05186.
GermOnline	ENSMUSG00000005973. Mus musculus.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13202. EF_hand_3. 2 hits. PF13499. EF_hand_5. 1 hit. [Graphical view]
SMART	SM00054. EFh. 3 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 3 hits. PS50222. EF_HAND_2. 6 hits. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RCN1. mouse.
NextBio	296990.
SOURCE	Search...

Entry information

Entry name

RCN1_MOUSE

Accession

Primary (citable) accession number: Q05186
Secondary accession number(s): Q3TVU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program