Reviewed, UniProtKB/Swiss-Prot Q05183 (PHT3_PSEPU)

Names and origin

Protein names

Recommended name:
    Phthalate 4,5-dioxygenase oxygenase subunit
    EC=1.14.12.7

Gene names

Name:

pht3

Encoded on

Plasmid PHT

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Phthalate + NADH + O(2) = cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,2-dicarboxylate + NAD(+).
Cofactor	Binds 1 2Fe-2S cluster Probable. Binds 1 iron ion Probable.
Pathway	Xenobiotic degradation; phtalic acid degradation; 3,4-dihydroxybenzoate from phtalic acid: step 1/3.
Subunit structure	This dioxygenase system consists of two proteins: phthalate oxygenase and phthalate oxygenase reductase.
Induction	Induced by phthalate and repressed by glucose.
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family. Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Dioxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW phthalate 4,5-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	Phthalate 4,5-dioxygenase oxygenase subunit		PRO_0000085056
Regions
	Domain	27 – 134	108	Rieske
Sites
	Metal binding	70	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	72	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	89	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	92	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	181	1	Iron By similarity
	Metal binding	186	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05183-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 57D14F0D70E8D0DA	FASTA	439	49,298
	10 20 30 40 50 60 MLTPEENLLL CRVEGDAPMG QMMRRHWTPV CLLEEVSEPD GTPVRARLFG EDLVVFRDTD 70 80 90 100 110 120 GRVGVMDEYC PHRRVSLIYG RNENSGLRCL YHGWKMDVDG NVVEMVSEPA ASNMCQKVKH 130 140 150 160 170 180 TAYKTREWGG FVWAYMGPQD AIPEFVPPAW APHEHVRVSI AKAIIPCNWA QILEGAIDSA 190 200 210 220 230 240 HSSSLHSSDF VPARVGGAEA TSKNWLRPST DKAPRMQVER TSYGFRYAAL RRPIQNAATS 250 260 270 280 290 300 EYVRSTVFVA PATALIPPNN LYNVANINVP IDDTHTAFYF MAWGNPDNTP ETETWRKFLG 310 320 330 340 350 360 QQVGIDLDDS YRPLRNDGNR FFQDREAMKN GNFTGIKGFP NQDIAMWVTM GPIADRSDER 370 380 390 400 410 420 LGASDLAVVE FRRVMLDALA AFQAGESAIG TGEKAIPSRI CSFQAIVSKD IDWRDYQARY 430 VWALDDANIV AEPDYEVHT

References

[1]	"Genes in PHT plasmid encoding the initial degradation pathway of phthalate in Pseudomonas putida." Nomura Y., Nakagawa M., Ogawa N., Harashima S., Oshima Y. J. Ferment. Bioeng. 74:333-344(1992) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NMH102-2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13229 Genomic DNA. Translation: BAA02511.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR005806. Rieske_reg. IPR015881. Ring-hydroxy_dOase_2Fe2S_BS. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
Pfam	PF00355. Rieske. 1 hit. [Graphical view]
PROSITE	PS51296. RIESKE. 1 hit. PS00570. RING_HYDROXYL_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHT3_PSEPU

Accession

Primary (citable) accession number: Q05183

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)