Reviewed, UniProtKB/Swiss-Prot Q05182 (PHT2_PSEPU)

Names and origin

Protein names

Recommended name:
    Phthalate 4,5-dioxygenase oxygenase reductase subunit
    EC=1.14.12.7

Gene names

Name:

pht2

Encoded on

Plasmid PHT

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Phthalate + NADH + O2 = cis-4,5-dihydroxycyclohexa-16,2-diene-1,2-dicarboxylate + NAD+.
Cofactor	FMN By similarity.
Pathway	Xenobiotic degradation; phthalate degradation; 3,4-dihydroxybenzoate from phthalate: step 1/3.
Subunit structure	This dioxygenase system consists of two proteins: phthalate oxygenase and phthalate oxygenase reductase.
Induction	Induced by phthalate and repressed by glucose.
Sequence similarities	Belongs to the PDR/vanB family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism Electron transport Transport
Ligand	2Fe-2S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Monooxygenase Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW phthalate 4,5-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Phthalate 4,5-dioxygenase oxygenase reductase subunit		PRO_0000189400
Regions
	Domain	9 – 111	103	FAD-binding FR-type
	Domain	241 – 324	84	2Fe-2S ferredoxin-type
	Nucleotide binding	115 – 229	115	NAD By similarity
Sites
	Metal binding	275	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	280	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	283	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	311	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05182-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2D211236644E1956	FASTA	324	36,000
	10 20 30 40 50 60 MSSSEQLDDG FTGLKVIAKT EIAQGIFRFE LAHPQGMLLP AFTAGAHLRV RVPNGSIRNY 70 80 90 100 110 120 SLSNDPQERE RYVIAVKRDA NGRGGSVSMA DDIEAGDLLP VATPQNEFEL IENARQFIFV 130 140 150 160 170 180 AGGIGITPIL SMMRHLKAST DLPFKLYYCT RNPELTAFRD ELLGAEFANT VVIHHDFGNR 190 200 210 220 230 240 ADAYDFWPVF DKPSSGTHVY CCGPRPLMDS VLDMTGHWPP GSIHFESFGV DQSRFAENRP 250 260 270 280 290 300 FSVTLGRSGI DLEIPVDRSI LEVLRDNGIR APSSCESGTC GSCRTRLIEG DVEHRDMVLR 310 320 EDEQHDQIMI CVSRARNDVL VLDL

References

[1]	"Genes in PHT plasmid encoding the initial degradation pathway of phthalate in Pseudomonas putida." Nomura Y., Nakagawa M., Ogawa N., Harashima S., Oshima Y. J. Ferment. Bioeng. 74:333-344(1992) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NMH102-2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13229 Genomic DNA. Translation: BAA02510.1.
3D structure databases
SMR	Q05182. Positions 8-324.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.12.7. 403.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR012675. b-grasp_ferredoxin-like. IPR017927. Fd_Rdtase_FAD-bd. IPR001041. Ferredoxin. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD_bd. IPR000951. Ph_dOase_redase_FPNCR. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00111. Fer2. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00409. PHDIOXRDTASE.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHT2_PSEPU

Accession

Primary (citable) accession number: Q05182

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)