Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q05166

- NUP59_YEAST

UniProt

Q05166 - NUP59_YEAST

Protein

Nucleoporin ASM4

Gene

ASM4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). May have a mitosis control function By similarity.By similarity

    GO - Molecular functioni

    1. nucleocytoplasmic transporter activity Source: SGD
    2. phospholipid binding Source: SGD
    3. protein binding Source: IntAct
    4. single-stranded DNA binding Source: SGD

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. mitotic spindle assembly checkpoint Source: SGD
    3. mRNA export from nucleus in response to heat stress Source: SGD
    4. nuclear pore organization Source: SGD
    5. protein transport Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, mRNA transport, Protein transport, Translocation, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29496-MONOMER.

    Protein family/group databases

    TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoporin ASM4
    Alternative name(s):
    Nuclear pore protein NUP59
    Gene namesi
    Name:ASM4
    Synonyms:NUP59
    Ordered Locus Names:YDL088C
    ORF Names:D2420
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL088c.
    SGDiS000002246. ASM4.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear membrane Source: UniProtKB-SubCell
    2. nuclear pore Source: SGD
    3. nuclear pore central transport channel Source: SGD

    Keywords - Cellular componenti

    Membrane, Nuclear pore complex, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 528528Nucleoporin ASM4PRO_0000204878Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei458 – 4581Phosphoserine2 Publications
    Modified residuei464 – 4641Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated by CDC28.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ05166.
    PaxDbiQ05166.
    PeptideAtlasiQ05166.

    Expressioni

    Gene expression databases

    GenevestigatoriQ05166.

    Interactioni

    Subunit structurei

    The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. ASM4 may form a subcomplex with NUP53, NDC1, and NUP170.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    NDC1P325003EBI-3035,EBI-11950

    Protein-protein interaction databases

    BioGridi31972. 79 interactions.
    DIPiDIP-1466N.
    IntActiQ05166. 13 interactions.
    MINTiMINT-389482.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05166.
    SMRiQ05166. Positions 270-395.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati2 – 32FG 11 Publication
    Repeati61 – 622FG 21 Publication
    Repeati195 – 1962FG 31 Publication
    Domaini265 – 394130RRM Nup35-typePROSITE-ProRule annotationAdd
    BLAST
    Repeati274 – 2752FG 41 Publication
    Repeati291 – 2922FG 51 Publication
    Repeati523 – 5242FG 61 Publication

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili490 – 51021Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi19 – 4931Gln-richAdd
    BLAST
    Compositional biasi28 – 4619Poly-GlnAdd
    BLAST
    Compositional biasi63 – 178116Asn-richAdd
    BLAST
    Compositional biasi84 – 874Poly-Asn

    Domaini

    Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side.
    The RRM Nup35-type domain might be involved in the control of mitosis.

    Sequence similaritiesi

    Contains 1 RRM Nup35-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiNOG43865.
    GeneTreeiENSGT00530000067349.
    HOGENOMiHOG000113890.
    OMAiTRNENTI.
    OrthoDBiEOG7S4XG6.

    Family and domain databases

    InterProiIPR007846. RRM_NUP35_dom.
    [Graphical view]
    PfamiPF05172. Nup35_RRM. 1 hit.
    [Graphical view]
    PROSITEiPS51472. RRM_NUP35. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q05166-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFGIRSGNNN GGFTNLTSQA PQTTQMFQSQ SQLQPQPQPQ PQQQQQHLQF    50
    NGSSDASSLR FGNSLSNTVN ANNYSSNIGN NSINNNNIKN GTNNISQHGQ 100
    GNNPSWVNNP KKRFTPHTVI RRKTTKQNSS SDINQNDDSS SMNATMRNFS 150
    KQNQDSKHNE RNKSAANNDI NSLLSNFNDI PPSVTLQDWQ REDEFGSIPS 200
    LTTQFVTDKY TAKKTNRSAY DSKNTPNVFD KDSYVRIANI EQNHLDNNYN 250
    TAETNNKVHE TSSKSSSLSA IIVFGYPESI SNELIEHFSH FGHIMEDFQV 300
    LRLGRGINPN TFRIFHNHDT GCDENDSTVN KSITLKGRNN ESNNKKYPIF 350
    TGESWVKLTY NSPSSALRAL QENGTIFRGS LIGCIPYSKN AVEQLAGCKI 400
    DNVDDIGEFN VSMYQNSSTS STSNTPSPPN VIITDGTLLR EDDNTPAGHA 450
    GNPTNISSPI VANSPNKRLD VIDGKLPFMQ NAGPNSNIPN LLRNLESKMR 500
    QQEAKYRNNE PAGFTHKLSN WLFGWNDL 528
    Length:528
    Mass (Da):58,793
    Last modified:November 1, 1997 - v1
    Checksum:i84FE1F07FC1B0173
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti114 – 1141F → L in CAA54130. (PubMed:7862092)Curated
    Sequence conflicti171 – 1711N → S in AAT92991. (PubMed:17322287)Curated
    Sequence conflicti446 – 52883PAGHA…GWNDL → LPVMLVIQQIFQVQ in CAA54130. (PubMed:7862092)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76709 Genomic DNA. Translation: CAA54130.1.
    X95644 Genomic DNA. Translation: CAA64923.1.
    Z74136 Genomic DNA. Translation: CAA98654.1.
    AY692972 Genomic DNA. Translation: AAT92991.1.
    BK006938 Genomic DNA. Translation: DAA11770.1.
    PIRiS67624.
    RefSeqiNP_010195.1. NM_001180147.1.

    Genome annotation databases

    EnsemblFungiiYDL088C; YDL088C; YDL088C.
    GeneIDi851470.
    KEGGisce:YDL088C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76709 Genomic DNA. Translation: CAA54130.1 .
    X95644 Genomic DNA. Translation: CAA64923.1 .
    Z74136 Genomic DNA. Translation: CAA98654.1 .
    AY692972 Genomic DNA. Translation: AAT92991.1 .
    BK006938 Genomic DNA. Translation: DAA11770.1 .
    PIRi S67624.
    RefSeqi NP_010195.1. NM_001180147.1.

    3D structure databases

    ProteinModelPortali Q05166.
    SMRi Q05166. Positions 270-395.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31972. 79 interactions.
    DIPi DIP-1466N.
    IntActi Q05166. 13 interactions.
    MINTi MINT-389482.

    Protein family/group databases

    TCDBi 1.I.1.1.1. the nuclear pore complex (npc) family.

    Proteomic databases

    MaxQBi Q05166.
    PaxDbi Q05166.
    PeptideAtlasi Q05166.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL088C ; YDL088C ; YDL088C .
    GeneIDi 851470.
    KEGGi sce:YDL088C.

    Organism-specific databases

    CYGDi YDL088c.
    SGDi S000002246. ASM4.

    Phylogenomic databases

    eggNOGi NOG43865.
    GeneTreei ENSGT00530000067349.
    HOGENOMi HOG000113890.
    OMAi TRNENTI.
    OrthoDBi EOG7S4XG6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29496-MONOMER.

    Miscellaneous databases

    NextBioi 968762.

    Gene expression databases

    Genevestigatori Q05166.

    Family and domain databases

    InterProi IPR007846. RRM_NUP35_dom.
    [Graphical view ]
    Pfami PF05172. Nup35_RRM. 1 hit.
    [Graphical view ]
    PROSITEi PS51472. RRM_NUP35. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Suppressors of thermosensitive mutations in the DNA polymerase delta gene of Saccharomyces cerevisiae."
      Giot L., Simon M., Dubois C., Faye G.
      Mol. Gen. Genet. 246:212-222(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames."
      Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A.
      Yeast 12:1377-1384(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p."
      Marelli M., Aitchison J.D., Wozniak R.W.
      J. Cell Biol. 143:1813-1830(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCOMPLEX WITH NUP170 AND NUP53, INTERACTION WITH KARYOPHERIN PSE1.
    7. "The yeast nuclear pore complex: composition, architecture, and transport mechanism."
      Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
      J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded."
      Denning D.P., Patel S.S., Uversky V., Fink A.L., Rexach M.
      Proc. Natl. Acad. Sci. U.S.A. 100:2450-2455(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, FG REPEAT STRUCTURE.
    10. Cited for: INTERACTION.
    11. "A comprehensive two-hybrid analysis to explore the yeast protein interactome."
      Ito T., Chiba T., Ozawa R., Yoshida M., Hattori M., Sakaki Y.
      Proc. Natl. Acad. Sci. U.S.A. 98:4569-4574(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION.
    12. "Peering through the pore: nuclear pore complex structure, assembly, and function."
      Suntharalingam M., Wente S.R.
      Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    13. Cited for: PHOSPHORYLATION BY CDC28.
    14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458 AND SER-464, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNUP59_YEAST
    AccessioniPrimary (citable) accession number: Q05166
    Secondary accession number(s): D6VRR0, E9P903, Q12456
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2740 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3