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Q05166 (NUP59_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoporin ASM4
Alternative name(s):
Nuclear pore protein NUP59
Gene names
Name:ASM4
Synonyms:NUP59
Ordered Locus Names:YDL088C
ORF Names:D2420
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length528 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). May have a mitosis control function By similarity. Ref.6 Ref.9

Subunit structure

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. ASM4 may form a subcomplex with NUP53, NDC1, and NUP170. Ref.7

Subcellular location

Nucleusnuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note: Symmetric distribution.

Domain

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side.

The RRM Nup35-type domain might be involved in the control of mitosis.

Post-translational modification

Phosphorylated by CDC28. Ref.13

Miscellaneous

Present with 2740 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 RRM Nup35-type domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

NDC1P325003EBI-3035,EBI-11950

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 528528Nucleoporin ASM4
PRO_0000204878

Regions

Repeat2 – 32FG 1
Repeat61 – 622FG 2
Repeat195 – 1962FG 3
Domain265 – 394130RRM Nup35-type
Repeat274 – 2752FG 4
Repeat291 – 2922FG 5
Repeat523 – 5242FG 6
Coiled coil490 – 51021 Potential
Compositional bias19 – 4931Gln-rich
Compositional bias28 – 4619Poly-Gln
Compositional bias63 – 178116Asn-rich
Compositional bias84 – 874Poly-Asn

Amino acid modifications

Modified residue4581Phosphoserine Ref.15
Modified residue4641Phosphoserine Ref.15

Experimental info

Sequence conflict1141F → L in CAA54130. Ref.1
Sequence conflict1711N → S in AAT92991. Ref.5
Sequence conflict446 – 52883PAGHA…GWNDL → LPVMLVIQQIFQVQ in CAA54130. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q05166 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 84FE1F07FC1B0173

FASTA52858,793
        10         20         30         40         50         60 
MFGIRSGNNN GGFTNLTSQA PQTTQMFQSQ SQLQPQPQPQ PQQQQQHLQF NGSSDASSLR 

        70         80         90        100        110        120 
FGNSLSNTVN ANNYSSNIGN NSINNNNIKN GTNNISQHGQ GNNPSWVNNP KKRFTPHTVI 

       130        140        150        160        170        180 
RRKTTKQNSS SDINQNDDSS SMNATMRNFS KQNQDSKHNE RNKSAANNDI NSLLSNFNDI 

       190        200        210        220        230        240 
PPSVTLQDWQ REDEFGSIPS LTTQFVTDKY TAKKTNRSAY DSKNTPNVFD KDSYVRIANI 

       250        260        270        280        290        300 
EQNHLDNNYN TAETNNKVHE TSSKSSSLSA IIVFGYPESI SNELIEHFSH FGHIMEDFQV 

       310        320        330        340        350        360 
LRLGRGINPN TFRIFHNHDT GCDENDSTVN KSITLKGRNN ESNNKKYPIF TGESWVKLTY 

       370        380        390        400        410        420 
NSPSSALRAL QENGTIFRGS LIGCIPYSKN AVEQLAGCKI DNVDDIGEFN VSMYQNSSTS 

       430        440        450        460        470        480 
STSNTPSPPN VIITDGTLLR EDDNTPAGHA GNPTNISSPI VANSPNKRLD VIDGKLPFMQ 

       490        500        510        520 
NAGPNSNIPN LLRNLESKMR QQEAKYRNNE PAGFTHKLSN WLFGWNDL 

« Hide

References

« Hide 'large scale' references
[1]"Suppressors of thermosensitive mutations in the DNA polymerase delta gene of Saccharomyces cerevisiae."
Giot L., Simon M., Dubois C., Faye G.
Mol. Gen. Genet. 246:212-222(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of a 16,691 bp segment of Saccharomyces cerevisiae chromosome IV identifies the DUN1, PMT1, PMT5, SRP14 and DPR1 genes, and five new open reading frames."
Boskovic J., Soler-Mira A., Garcia-Cantalejo J.M., Ballesta J.P.G., Jimenez A., Remacha M.A.
Yeast 12:1377-1384(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p."
Marelli M., Aitchison J.D., Wozniak R.W.
J. Cell Biol. 143:1813-1830(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCOMPLEX WITH NUP170 AND NUP53, INTERACTION WITH KARYOPHERIN PSE1.
[7]"The yeast nuclear pore complex: composition, architecture, and transport mechanism."
Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T.
J. Cell Biol. 148:635-651(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Disorder in the nuclear pore complex: the FG repeat regions of nucleoporins are natively unfolded."
Denning D.P., Patel S.S., Uversky V., Fink A.L., Rexach M.
Proc. Natl. Acad. Sci. U.S.A. 100:2450-2455(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, FG REPEAT STRUCTURE.
[10]"A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae."
Uetz P., Giot L., Cagney G., Mansfield T.A., Judson R.S., Knight J.R., Lockshon D., Narayan V., Srinivasan M., Pochart P., Qureshi-Emili A., Li Y., Godwin B., Conover D., Kalbfleisch T., Vijayadamodar G., Yang M., Johnston M., Fields S., Rothberg J.M.
Nature 403:623-627(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION.
[11]"A comprehensive two-hybrid analysis to explore the yeast protein interactome."
Ito T., Chiba T., Ozawa R., Yoshida M., Hattori M., Sakaki Y.
Proc. Natl. Acad. Sci. U.S.A. 98:4569-4574(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION.
[12]"Peering through the pore: nuclear pore complex structure, assembly, and function."
Suntharalingam M., Wente S.R.
Dev. Cell 4:775-789(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[13]"Targets of the cyclin-dependent kinase Cdk1."
Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O.
Nature 425:859-864(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION BY CDC28.
[14]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-458 AND SER-464, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76709 Genomic DNA. Translation: CAA54130.1.
X95644 Genomic DNA. Translation: CAA64923.1.
Z74136 Genomic DNA. Translation: CAA98654.1.
AY692972 Genomic DNA. Translation: AAT92991.1.
BK006938 Genomic DNA. Translation: DAA11770.1.
PIRS67624.
RefSeqNP_010195.1. NM_001180147.1.

3D structure databases

ProteinModelPortalQ05166.
SMRQ05166. Positions 270-395.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31972. 79 interactions.
DIPDIP-1466N.
IntActQ05166. 13 interactions.
MINTMINT-389482.

Protein family/group databases

TCDB1.I.1.1.1. the nuclear pore complex (npc) family.

Proteomic databases

MaxQBQ05166.
PaxDbQ05166.
PeptideAtlasQ05166.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL088C; YDL088C; YDL088C.
GeneID851470.
KEGGsce:YDL088C.

Organism-specific databases

CYGDYDL088c.
SGDS000002246. ASM4.

Phylogenomic databases

eggNOGNOG43865.
GeneTreeENSGT00530000067349.
HOGENOMHOG000113890.
OMATRNENTI.
OrthoDBEOG7S4XG6.

Enzyme and pathway databases

BioCycYEAST:G3O-29496-MONOMER.

Gene expression databases

GenevestigatorQ05166.

Family and domain databases

InterProIPR007846. RRM_NUP35_dom.
[Graphical view]
PfamPF05172. Nup35_RRM. 1 hit.
[Graphical view]
PROSITEPS51472. RRM_NUP35. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968762.

Entry information

Entry nameNUP59_YEAST
AccessionPrimary (citable) accession number: Q05166
Secondary accession number(s): D6VRR0, E9P903, Q12456
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families