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Protein

Haze protective factor 1

Gene

HPF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cell wall organization and biosynthesis.3 Publications

GO - Molecular functioni

  • glucosidase activity Source: SGD

GO - Biological processi

  • fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-33543-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Haze protective factor 1
Gene namesi
Name:HPF1
Ordered Locus Names:YOL155C
ORF Names:AOF1001
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL155C.
SGDiS000005515. HPF1.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • extracellular region Source: SGD
  • fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

Pathology & Biotechi

Biotechnological usei

Probable mannoprotein called haze protective factor from wine that is able to prevent visible wine protein haze formation. This mannoprotein showed haze-protective activity against wine proteins and BSA when either was heated in white wine.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000026817724 – 946Haze protective factor 1Add BLAST923
PropeptideiPRO_0000268178947 – 967Removed in mature formSequence analysisAdd BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...)Sequence analysis1
Glycosylationi35N-linked (GlcNAc...)Sequence analysis1
Glycosylationi493N-linked (GlcNAc...)Sequence analysis1
Glycosylationi601N-linked (GlcNAc...)Sequence analysis1
Glycosylationi638N-linked (GlcNAc...)Sequence analysis1
Lipidationi946GPI-anchor amidated alanineSequence analysis1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ05164.
PRIDEiQ05164.

Interactioni

Protein-protein interaction databases

BioGridi34263. 26 interactors.
MINTiMINT-4806805.

Structurei

3D structure databases

ProteinModelPortaliQ05164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati93 – 1051-11 PublicationAdd BLAST13
Repeati106 – 1181-21 PublicationAdd BLAST13
Repeati119 – 1311-31 PublicationAdd BLAST13
Repeati132 – 1441-41 PublicationAdd BLAST13
Repeati153 – 1651-51 PublicationAdd BLAST13
Repeati166 – 1781-61 PublicationAdd BLAST13
Repeati179 – 1911-71 PublicationAdd BLAST13
Repeati192 – 2041-81 PublicationAdd BLAST13
Repeati205 – 2171-91 PublicationAdd BLAST13
Repeati218 – 2301-10; approximate1 PublicationAdd BLAST13
Repeati234 – 2471-11; approximate1 PublicationAdd BLAST14
Repeati248 – 2591-12; approximate1 PublicationAdd BLAST12
Repeati266 – 2781-131 PublicationAdd BLAST13
Repeati745 – 7802-11 PublicationAdd BLAST36
Repeati781 – 8152-21 PublicationAdd BLAST35
Repeati816 – 8542-31 PublicationAdd BLAST39
Repeati855 – 8932-41 PublicationAdd BLAST39
Repeati894 – 9022-5; truncated1 Publication9

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 27813 X approximate repeats, Ser-richAdd BLAST186
Regioni745 – 9024.5 X approximate tandem repeats, Thr-richAdd BLAST158

Domaini

The number of the intragenic tandem repeats varies between different S.cerevisiae strains.

Sequence similaritiesi

Belongs to the SRP1/TIP1 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00730000112313.
InParanoidiQ05164.
OMAiYRTSKET.
OrthoDBiEOG092C1MNS.

Family and domain databases

InterProiIPR021031. Hyphal-reg_cell_wall_N.
[Graphical view]
PfamiPF11765. Hyphal_reg_CWP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNRFNKLQA ALALVLYSQS ALGQYYTNSS SIASNSSTAV SSTSSGSVSI
60 70 80 90 100
SSSIELTSST SDVSSSLTEL TSSSTEVSSS IAPSTSSSEV SSSITSSGSS
110 120 130 140 150
VSGSSSITSS GSSVSSSSSA TESGSSASGS SSATESGSSV SGSSTSITSG
160 170 180 190 200
SSSATESGSS VSGSTSATES GSSASGSSSA TESGSSASGS SSATESGSSV
210 220 230 240 250
SGSSSATESG SSVSGSSSAT ESGSASSVPS SSGSVTESGS SSSASESSIT
260 270 280 290 300
QSGTASGSSA SSTSGSVTQS GSSVSGSSAS SAPGISSSIP QSTSSASTAS
310 320 330 340 350
GSITSGTLSS ITSSASSATA TASNSLSSSD GTIYLPSTTI SGDITLTGSV
360 370 380 390 400
IATEAVEVAA GGKLTLLDGD KYVFSADFII HGGVFVEKSK PTYPGTEFDI
410 420 430 440 450
SGENFDVSGT FNAEEPAASS ASAYSFTPGS FDNSGDISLS LSESTKGEVT
460 470 480 490 500
FSPYSNSGAF SFSNAILNGG SVSGLQRRAE SGSVNNGEIN LENGSTYVVV
510 520 530 540 550
EPVSGSGTIN IISGNLYLHY PDTFTGQTVV FKGEGVLAVD PTETNTTPIP
560 570 580 590 600
VVGYTGENQI AITADVTALS YDSATGVLTA TQGNSQFSFS IGTGFSSSGF
610 620 630 640 650
NVSEGTFAGA YAYYLNYGGV VASSATPSST STTSGATNST SGSTSFGASV
660 670 680 690 700
TGSTASTSFG ASVTGSTAST LISGSPSVYT TTLTYATTTS TVVVSCSETT
710 720 730 740 750
DSNGNVYTIT TTVPCSSTTA TITSCDETGC HVTTSTGTVA TETVSSKSYT
760 770 780 790 800
TVTVTHCDNN GCNTKTVTSE CPEETSATTT SPKSYTTVTV THCDDNGCNT
810 820 830 840 850
KTVTSEAPEA TTTTVSPKTY TTATVTQCDD NGCSTKTVTS EAPKETSETS
860 870 880 890 900
ETSAAPKTYT TATVTQCDDN GCNVKIITSQ IPEATSTVTA TSASPKSYTT
910 920 930 940 950
VTSEGSKATS LTTAISKASS AISTYSKSAA PIKTSTGIIV QSEGIAAGLN
960
ANTLNALVGI FVLAFFN
Length:967
Mass (Da):94,706
Last modified:December 12, 2006 - v2
Checksum:i7BFC01EA243A561E
GO

Sequence cautioni

The sequence CAA61860 differs from that shown. Erroneous sequence assembling in the Ser-rich region leading to a longer sequence.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89715 Genomic DNA. Translation: CAA61860.1. Sequence problems.
Z74897 Genomic DNA. Translation: CAA99177.1.
BK006948 Genomic DNA. Translation: DAA10632.1.
PIRiS66852.
RefSeqiNP_014487.1. NM_001183408.1.

Genome annotation databases

EnsemblFungiiYOL155C; YOL155C; YOL155C.
GeneIDi854010.
KEGGisce:YOL155C.

Cross-referencesi

Web resourcesi

Protein Spotlight

Of froth and haze - Issue 78 of January 2007

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89715 Genomic DNA. Translation: CAA61860.1. Sequence problems.
Z74897 Genomic DNA. Translation: CAA99177.1.
BK006948 Genomic DNA. Translation: DAA10632.1.
PIRiS66852.
RefSeqiNP_014487.1. NM_001183408.1.

3D structure databases

ProteinModelPortaliQ05164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34263. 26 interactors.
MINTiMINT-4806805.

Proteomic databases

MaxQBiQ05164.
PRIDEiQ05164.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL155C; YOL155C; YOL155C.
GeneIDi854010.
KEGGisce:YOL155C.

Organism-specific databases

EuPathDBiFungiDB:YOL155C.
SGDiS000005515. HPF1.

Phylogenomic databases

GeneTreeiENSGT00730000112313.
InParanoidiQ05164.
OMAiYRTSKET.
OrthoDBiEOG092C1MNS.

Enzyme and pathway databases

BioCyciYEAST:G3O-33543-MONOMER.

Miscellaneous databases

PROiQ05164.

Family and domain databases

InterProiIPR021031. Hyphal-reg_cell_wall_N.
[Graphical view]
PfamiPF11765. Hyphal_reg_CWP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHPF1_YEAST
AccessioniPrimary (citable) accession number: Q05164
Secondary accession number(s): D6W1R6, Q08294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: November 2, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.