Reviewed,
UniProtKB/Swiss-Prot Q05164 (HPF1_YEAST)
Last modified
November 24, 2009.
Version 52.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Haze protective factor 1 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 967 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in cell wall organization and biosynthesis. Ref.3 Ref.4 Ref.5 |
| Subcellular location | Secreted › cell wall. Membrane; Lipid-anchor › GPI-anchor. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP), enriched at bud sites and their neighborhood. Ref.4 Ref.5 Ref.6 |
| Domain | The number of the intragenic tandem repeats varies between different S.cerevisiae strains. |
| Post-translational modification | The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved of and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer. |
| Biotechnological use | Probable mannoprotein called haze protective factor from wine that is able to prevent visible wine protein haze formation. This mannoprotein showed haze-protective activity against wine proteins and BSA when either was heated in white wine. Ref.3 Ref.4 |
| Sequence similarities | Belongs to the SRP1/TIP1 family. |
| Sequence caution | The sequence CAA61860.1 differs from that shown. Reason: Miscellaneous discrepancy. Erroneous sequence assembling in the Ser-rich region leading to a longer sequence. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell wall biogenesis/degradation |
| Cellular component | Cell wall Membrane Secreted |
| Domain | Repeat Signal |
| PTM | GPI-anchor Glycoprotein Lipoprotein |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | cell wall organization Ref.5 Inferred from genetic interaction. Source: SGD |
| Cellular component | anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Ref.6Inferred from direct assay. Source: SGD fungal-type cell wall Ref.5Inferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 23 | 23 | Potential | ||||||
| Chain | 24 – 946 | 923 | Haze protective factor 1 | PRO_0000268177 | |||||
| Propeptide | 947 – 967 | 21 | Removed in mature form Potential | PRO_0000268178 | |||||
Regions | |||||||||
| Repeat | 93 – 105 | 13 | 1-1 | ||||||
| Repeat | 106 – 118 | 13 | 1-2 | ||||||
| Repeat | 119 – 131 | 13 | 1-3 | ||||||
| Repeat | 132 – 144 | 13 | 1-4 | ||||||
| Repeat | 153 – 165 | 13 | 1-5 | ||||||
| Repeat | 166 – 178 | 13 | 1-6 | ||||||
| Repeat | 179 – 191 | 13 | 1-7 | ||||||
| Repeat | 192 – 204 | 13 | 1-8 | ||||||
| Repeat | 205 – 217 | 13 | 1-9 | ||||||
| Repeat | 218 – 230 | 13 | 1-10; approximate | ||||||
| Repeat | 234 – 247 | 14 | 1-11; approximate | ||||||
| Repeat | 248 – 259 | 12 | 1-12; approximate | ||||||
| Repeat | 266 – 278 | 13 | 1-13 | ||||||
| Repeat | 745 – 780 | 36 | 2-1 | ||||||
| Repeat | 781 – 815 | 35 | 2-2 | ||||||
| Repeat | 816 – 854 | 39 | 2-3 | ||||||
| Repeat | 855 – 893 | 39 | 2-4 | ||||||
| Repeat | 894 – 902 | 9 | 2-5; truncated | ||||||
| Region | 93 – 278 | 186 | 13 X approximate repeats, Ser-rich | ||||||
| Region | 745 – 902 | 158 | 4.5 X approximate tandem repeats, Thr-rich | ||||||
Amino acid modifications | |||||||||
| Lipidation | 946 | 1 | GPI-anchor amidated alanine Potential | ||||||
| Glycosylation | 28 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 35 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 493 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 601 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 638 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Analysis of the DNA sequence of a 15,500 bp fragment near the left telomere of chromosome XV from Saccharomyces cerevisiae reveals a putative sugar transporter, a carboxypeptidase homologue and two new open reading frames." Gamo F.-J., Lafuente M.J., Casamayor A., Arino J., Aldea M., Casas C., Herrero E., Gancedo C. Yeast 12:709-714(1996) [PubMed: 8810044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K.Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [3] | "Reducing haziness in white wine by overexpression of Saccharomyces cerevisiae genes YOL155c and YDR055w." Brown S.L., Stockdale V.J., Pettolino F., Pocock K.F., de Barros Lopes M., Williams P.J., Bacic A., Fincher G.B., Hoej P.B., Waters E.J. Appl. Microbiol. Biotechnol. 73:1363-1376(2007) [PubMed: 17024473] [Abstract] Cited for: PROTEIN SEQUENCE OF 479-504, FUNCTION, BIOTECHNOLOGY. |
| [4] | "Saccharomyces cerevisiae mannoproteins that protect wine from protein haze: evaluation of extraction methods and immunolocalization." Dupin I.V.S., Stockdale V.J., Williams P.J., Jones G.P., Markides A.J., Waters E.J. J. Agric. Food Chem. 48:1086-1095(2000) [PubMed: 10775354] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOTECHNOLOGY. |
| [5] | "Cooperative functions of the mannoprotein-encoding genes in the biogenesis and maintenance of the cell wall in Saccharomyces cerevisiae." Horie T., Isono K. Yeast 18:1493-1503(2001) [PubMed: 11748726] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
| [6] | "Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae." Terashima H., Fukuchi S., Nakai K., Arisawa M., Hamada K., Yabuki N., Kitada K. Curr. Genet. 40:311-316(2002) [PubMed: 11935221] [Abstract] Cited for: SUBCELLULAR LOCATION. |
| [7] | "Intragenic tandem repeats generate functional variability." Verstrepen K.J., Jansen A., Lewitter F., Fink G.R. Nat. Genet. 37:986-990(2005) [PubMed: 16086015] [Abstract] Cited for: REPEATS. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X89715 Genomic DNA. Translation: CAA61860.1. Sequence problems. Z74897 Genomic DNA. Translation: CAA99177.1. | |
| PIR | S66852. |
| RefSeq | NP_014487.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q05164. |
Proteomic databases | |
| PeptideAtlas | Q05164. |
Genome annotation databases | |
| Ensembl | YOL155C; YOL155C; YOL155C; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 854010. |
| KEGG | sce:YOL155C. |
| NMPDR | fig|4932.3.peg.5573. |
Organism-specific databases | |
| CYGD | YOL155c. |
| SGD | S000005515. HPF1. |
Phylogenomic databases | |
| HOGENOM | Q05164. |
| OMA | STAETFY |
| OrthoDB | EOG91VMK2 |
Gene expression databases | |
| ArrayExpress | Q05164. |
| Genevestigator | Q05164. |
| GermOnline | YOL155C. Saccharomyces cerevisiae. |
Family and domain databases | |
| PROSITE | PS00724. SRP1_TIP1. False negative. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 975524. |
Entry information
| Entry name | HPF1_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q05164 Secondary accession number(s): Q08294 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| Protein Spotlight Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


