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Q05164 (HPF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Haze protective factor 1
Gene names
Name:HPF1
Ordered Locus Names:YOL155C
ORF Names:AOF1001
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length967 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in cell wall organization and biosynthesis. Ref.4 Ref.5 Ref.6

Subcellular location

Secretedcell wall. Membrane; Lipid-anchorGPI-anchor. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP), enriched at bud sites and their neighborhood. Ref.5 Ref.6 Ref.7

Domain

The number of the intragenic tandem repeats varies between different S.cerevisiae strains.

Post-translational modification

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Biotechnological use

Probable mannoprotein called haze protective factor from wine that is able to prevent visible wine protein haze formation. This mannoprotein showed haze-protective activity against wine proteins and BSA when either was heated in white wine. Ref.4 Ref.5

Sequence similarities

Belongs to the SRP1/TIP1 family.

Sequence caution

The sequence CAA61860.1 differs from that shown. Reason: Erroneous sequence assembling in the Ser-rich region leading to a longer sequence.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 946923Haze protective factor 1
PRO_0000268177
Propeptide947 – 96721Removed in mature form Potential
PRO_0000268178

Regions

Repeat93 – 105131-1
Repeat106 – 118131-2
Repeat119 – 131131-3
Repeat132 – 144131-4
Repeat153 – 165131-5
Repeat166 – 178131-6
Repeat179 – 191131-7
Repeat192 – 204131-8
Repeat205 – 217131-9
Repeat218 – 230131-10; approximate
Repeat234 – 247141-11; approximate
Repeat248 – 259121-12; approximate
Repeat266 – 278131-13
Repeat745 – 780362-1
Repeat781 – 815352-2
Repeat816 – 854392-3
Repeat855 – 893392-4
Repeat894 – 90292-5; truncated
Region93 – 27818613 X approximate repeats, Ser-rich
Region745 – 9021584.5 X approximate tandem repeats, Thr-rich

Amino acid modifications

Lipidation9461GPI-anchor amidated alanine Potential
Glycosylation281N-linked (GlcNAc...) Potential
Glycosylation351N-linked (GlcNAc...) Potential
Glycosylation4931N-linked (GlcNAc...) Potential
Glycosylation6011N-linked (GlcNAc...) Potential
Glycosylation6381N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q05164 [UniParc].

Last modified December 12, 2006. Version 2.
Checksum: 7BFC01EA243A561E

FASTA96794,706
        10         20         30         40         50         60 
MFNRFNKLQA ALALVLYSQS ALGQYYTNSS SIASNSSTAV SSTSSGSVSI SSSIELTSST 

        70         80         90        100        110        120 
SDVSSSLTEL TSSSTEVSSS IAPSTSSSEV SSSITSSGSS VSGSSSITSS GSSVSSSSSA 

       130        140        150        160        170        180 
TESGSSASGS SSATESGSSV SGSSTSITSG SSSATESGSS VSGSTSATES GSSASGSSSA 

       190        200        210        220        230        240 
TESGSSASGS SSATESGSSV SGSSSATESG SSVSGSSSAT ESGSASSVPS SSGSVTESGS 

       250        260        270        280        290        300 
SSSASESSIT QSGTASGSSA SSTSGSVTQS GSSVSGSSAS SAPGISSSIP QSTSSASTAS 

       310        320        330        340        350        360 
GSITSGTLSS ITSSASSATA TASNSLSSSD GTIYLPSTTI SGDITLTGSV IATEAVEVAA 

       370        380        390        400        410        420 
GGKLTLLDGD KYVFSADFII HGGVFVEKSK PTYPGTEFDI SGENFDVSGT FNAEEPAASS 

       430        440        450        460        470        480 
ASAYSFTPGS FDNSGDISLS LSESTKGEVT FSPYSNSGAF SFSNAILNGG SVSGLQRRAE 

       490        500        510        520        530        540 
SGSVNNGEIN LENGSTYVVV EPVSGSGTIN IISGNLYLHY PDTFTGQTVV FKGEGVLAVD 

       550        560        570        580        590        600 
PTETNTTPIP VVGYTGENQI AITADVTALS YDSATGVLTA TQGNSQFSFS IGTGFSSSGF 

       610        620        630        640        650        660 
NVSEGTFAGA YAYYLNYGGV VASSATPSST STTSGATNST SGSTSFGASV TGSTASTSFG 

       670        680        690        700        710        720 
ASVTGSTAST LISGSPSVYT TTLTYATTTS TVVVSCSETT DSNGNVYTIT TTVPCSSTTA 

       730        740        750        760        770        780 
TITSCDETGC HVTTSTGTVA TETVSSKSYT TVTVTHCDNN GCNTKTVTSE CPEETSATTT 

       790        800        810        820        830        840 
SPKSYTTVTV THCDDNGCNT KTVTSEAPEA TTTTVSPKTY TTATVTQCDD NGCSTKTVTS 

       850        860        870        880        890        900 
EAPKETSETS ETSAAPKTYT TATVTQCDDN GCNVKIITSQ IPEATSTVTA TSASPKSYTT 

       910        920        930        940        950        960 
VTSEGSKATS LTTAISKASS AISTYSKSAA PIKTSTGIIV QSEGIAAGLN ANTLNALVGI 


FVLAFFN 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the DNA sequence of a 15,500 bp fragment near the left telomere of chromosome XV from Saccharomyces cerevisiae reveals a putative sugar transporter, a carboxypeptidase homologue and two new open reading frames."
Gamo F.-J., Lafuente M.J., Casamayor A., Arino J., Aldea M., Casas C., Herrero E., Gancedo C.
Yeast 12:709-714(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Reducing haziness in white wine by overexpression of Saccharomyces cerevisiae genes YOL155c and YDR055w."
Brown S.L., Stockdale V.J., Pettolino F., Pocock K.F., de Barros Lopes M., Williams P.J., Bacic A., Fincher G.B., Hoej P.B., Waters E.J.
Appl. Microbiol. Biotechnol. 73:1363-1376(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 479-504, FUNCTION, BIOTECHNOLOGY.
[5]"Saccharomyces cerevisiae mannoproteins that protect wine from protein haze: evaluation of extraction methods and immunolocalization."
Dupin I.V.S., Stockdale V.J., Williams P.J., Jones G.P., Markides A.J., Waters E.J.
J. Agric. Food Chem. 48:1086-1095(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOTECHNOLOGY.
[6]"Cooperative functions of the mannoprotein-encoding genes in the biogenesis and maintenance of the cell wall in Saccharomyces cerevisiae."
Horie T., Isono K.
Yeast 18:1493-1503(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]"Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae."
Terashima H., Fukuchi S., Nakai K., Arisawa M., Hamada K., Yabuki N., Kitada K.
Curr. Genet. 40:311-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"Intragenic tandem repeats generate functional variability."
Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REPEATS.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Of froth and haze - Issue 78 of January 2007

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89715 Genomic DNA. Translation: CAA61860.1. Sequence problems.
Z74897 Genomic DNA. Translation: CAA99177.1.
BK006948 Genomic DNA. Translation: DAA10632.1.
PIRS66852.
RefSeqNP_014487.1. NM_001183408.1.

3D structure databases

ProteinModelPortalQ05164.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34263. 27 interactions.
MINTMINT-4806805.

Proteomic databases

PeptideAtlasQ05164.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL155C; YOL155C; YOL155C.
GeneID854010.
KEGGsce:YOL155C.

Organism-specific databases

CYGDYOL155c.
SGDS000005515. HPF1.

Phylogenomic databases

GeneTreeENSGT00730000112313.
OMASTAETFY.
OrthoDBEOG7VQJPN.

Enzyme and pathway databases

BioCycYEAST:G3O-33543-MONOMER.

Gene expression databases

GenevestigatorQ05164.

Family and domain databases

ProtoNetSearch...

Other

NextBio975524.

Entry information

Entry nameHPF1_YEAST
AccessionPrimary (citable) accession number: Q05164
Secondary accession number(s): D6W1R6, Q08294
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: April 16, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries