Reviewed, UniProtKB/Swiss-Prot Q05164 (HPF1_YEAST)

Names and origin

Protein names

Recommended name:
    Haze protective factor 1

Gene names

Name:	HPF1
Ordered Locus Names:	YOL155C
ORF Names:	AOF1001

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	967 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in cell wall organization and biosynthesis. Ref.3 Ref.4 Ref.5
Subcellular location	Secreted › cell wall. Membrane; Lipid-anchor › GPI-anchor. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP), enriched at bud sites and their neighborhood. Ref.4 Ref.5 Ref.6
Domain	The number of the intragenic tandem repeats varies between different S.cerevisiae strains.
Post-translational modification	The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved of and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
Biotechnological use	Probable mannoprotein called haze protective factor from wine that is able to prevent visible wine protein haze formation. This mannoprotein showed haze-protective activity against wine proteins and BSA when either was heated in white wine. Ref.3 Ref.4
Sequence similarities	Belongs to the SRP1/TIP1 family.
Sequence caution	The sequence CAA61860.1 differs from that shown. Reason: Miscellaneous discrepancy. Erroneous sequence assembling in the Ser-rich region leading to a longer sequence.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Membrane Secreted
Domain	Repeat Signal
PTM	GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall organization Ref.5 Inferred from genetic interaction. Source: SGD
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Ref.6 Inferred from direct assay. Source: SGD fungal-type cell wall Ref.5 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 946	923	Haze protective factor 1		PRO_0000268177
	Propeptide	947 – 967	21	Removed in mature form Potential		PRO_0000268178
Regions
	Repeat	93 – 105	13	1-1
	Repeat	106 – 118	13	1-2
	Repeat	119 – 131	13	1-3
	Repeat	132 – 144	13	1-4
	Repeat	153 – 165	13	1-5
	Repeat	166 – 178	13	1-6
	Repeat	179 – 191	13	1-7
	Repeat	192 – 204	13	1-8
	Repeat	205 – 217	13	1-9
	Repeat	218 – 230	13	1-10; approximate
	Repeat	234 – 247	14	1-11; approximate
	Repeat	248 – 259	12	1-12; approximate
	Repeat	266 – 278	13	1-13
	Repeat	745 – 780	36	2-1
	Repeat	781 – 815	35	2-2
	Repeat	816 – 854	39	2-3
	Repeat	855 – 893	39	2-4
	Repeat	894 – 902	9	2-5; truncated
	Region	93 – 278	186	13 X approximate repeats, Ser-rich
	Region	745 – 902	158	4.5 X approximate tandem repeats, Thr-rich
Amino acid modifications
	Lipidation	946	1	GPI-anchor amidated alanine Potential
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Glycosylation	35	1	N-linked (GlcNAc...) Potential
	Glycosylation	493	1	N-linked (GlcNAc...) Potential
	Glycosylation	601	1	N-linked (GlcNAc...) Potential
	Glycosylation	638	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q05164-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: 7BFC01EA243A561E	FASTA	967	94,706
	10 20 30 40 50 60 MFNRFNKLQA ALALVLYSQS ALGQYYTNSS SIASNSSTAV SSTSSGSVSI SSSIELTSST 70 80 90 100 110 120 SDVSSSLTEL TSSSTEVSSS IAPSTSSSEV SSSITSSGSS VSGSSSITSS GSSVSSSSSA 130 140 150 160 170 180 TESGSSASGS SSATESGSSV SGSSTSITSG SSSATESGSS VSGSTSATES GSSASGSSSA 190 200 210 220 230 240 TESGSSASGS SSATESGSSV SGSSSATESG SSVSGSSSAT ESGSASSVPS SSGSVTESGS 250 260 270 280 290 300 SSSASESSIT QSGTASGSSA SSTSGSVTQS GSSVSGSSAS SAPGISSSIP QSTSSASTAS 310 320 330 340 350 360 GSITSGTLSS ITSSASSATA TASNSLSSSD GTIYLPSTTI SGDITLTGSV IATEAVEVAA 370 380 390 400 410 420 GGKLTLLDGD KYVFSADFII HGGVFVEKSK PTYPGTEFDI SGENFDVSGT FNAEEPAASS 430 440 450 460 470 480 ASAYSFTPGS FDNSGDISLS LSESTKGEVT FSPYSNSGAF SFSNAILNGG SVSGLQRRAE 490 500 510 520 530 540 SGSVNNGEIN LENGSTYVVV EPVSGSGTIN IISGNLYLHY PDTFTGQTVV FKGEGVLAVD 550 560 570 580 590 600 PTETNTTPIP VVGYTGENQI AITADVTALS YDSATGVLTA TQGNSQFSFS IGTGFSSSGF 610 620 630 640 650 660 NVSEGTFAGA YAYYLNYGGV VASSATPSST STTSGATNST SGSTSFGASV TGSTASTSFG 670 680 690 700 710 720 ASVTGSTAST LISGSPSVYT TTLTYATTTS TVVVSCSETT DSNGNVYTIT TTVPCSSTTA 730 740 750 760 770 780 TITSCDETGC HVTTSTGTVA TETVSSKSYT TVTVTHCDNN GCNTKTVTSE CPEETSATTT 790 800 810 820 830 840 SPKSYTTVTV THCDDNGCNT KTVTSEAPEA TTTTVSPKTY TTATVTQCDD NGCSTKTVTS 850 860 870 880 890 900 EAPKETSETS ETSAAPKTYT TATVTQCDDN GCNVKIITSQ IPEATSTVTA TSASPKSYTT 910 920 930 940 950 960 VTSEGSKATS LTTAISKASS AISTYSKSAA PIKTSTGIIV QSEGIAAGLN ANTLNALVGI FVLAFFN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the DNA sequence of a 15,500 bp fragment near the left telomere of chromosome XV from Saccharomyces cerevisiae reveals a putative sugar transporter, a carboxypeptidase homologue and two new open reading frames." Gamo F.-J., Lafuente M.J., Casamayor A., Arino J., Aldea M., Casas C., Herrero E., Gancedo C. Yeast 12:709-714(1996) [PubMed: 8810044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Reducing haziness in white wine by overexpression of Saccharomyces cerevisiae genes YOL155c and YDR055w." Brown S.L., Stockdale V.J., Pettolino F., Pocock K.F., de Barros Lopes M., Williams P.J., Bacic A., Fincher G.B., Hoej P.B., Waters E.J. Appl. Microbiol. Biotechnol. 73:1363-1376(2007) [PubMed: 17024473] [Abstract] Cited for: PROTEIN SEQUENCE OF 479-504, FUNCTION, BIOTECHNOLOGY.
[4]	"Saccharomyces cerevisiae mannoproteins that protect wine from protein haze: evaluation of extraction methods and immunolocalization." Dupin I.V.S., Stockdale V.J., Williams P.J., Jones G.P., Markides A.J., Waters E.J. J. Agric. Food Chem. 48:1086-1095(2000) [PubMed: 10775354] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOTECHNOLOGY.
[5]	"Cooperative functions of the mannoprotein-encoding genes in the biogenesis and maintenance of the cell wall in Saccharomyces cerevisiae." Horie T., Isono K. Yeast 18:1493-1503(2001) [PubMed: 11748726] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae." Terashima H., Fukuchi S., Nakai K., Arisawa M., Hamada K., Yabuki N., Kitada K. Curr. Genet. 40:311-316(2002) [PubMed: 11935221] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Intragenic tandem repeats generate functional variability." Verstrepen K.J., Jansen A., Lewitter F., Fink G.R. Nat. Genet. 37:986-990(2005) [PubMed: 16086015] [Abstract] Cited for: REPEATS.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Of froth and haze - Issue 78 of January 2007

Cross-references

Sequence databases
EMBLGenBankDDBJ	X89715 Genomic DNA. Translation: CAA61860.1. Sequence problems. Z74897 Genomic DNA. Translation: CAA99177.1.
PIR	S66852.
RefSeq	NP_014487.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q05164.
Proteomic databases
PeptideAtlas	Q05164.
Genome annotation databases
Ensembl	YOL155C; YOL155C; YOL155C; Saccharomyces cerevisiae. [Genome view]
GeneID	854010.
KEGG	sce:YOL155C.
NMPDR	fig|4932.3.peg.5573.
Organism-specific databases
CYGD	YOL155c.
SGD	S000005515. HPF1.
Phylogenomic databases
HOGENOM	Q05164.
OMA	STAETFY
OrthoDB	EOG91VMK2
Gene expression databases
ArrayExpress	Q05164.
Genevestigator	Q05164.
GermOnline	YOL155C. Saccharomyces cerevisiae.
Family and domain databases
PROSITE	PS00724. SRP1_TIP1. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	975524.

Entry information

Entry name

HPF1_YEAST

Accession

Primary (citable) accession number: Q05164
Secondary accession number(s): Q08294

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	December 12, 2006
Last modified:	November 24, 2009
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)