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Q05164

- HPF1_YEAST

UniProt

Q05164 - HPF1_YEAST

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Protein
Haze protective factor 1
Gene
HPF1, YOL155C, AOF1001
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in cell wall organization and biosynthesis.3 Publications

GO - Molecular functioni

  1. glucosidase activity Source: SGD

GO - Biological processi

  1. fungal-type cell wall organization Source: SGD
  2. metabolic process Source: GOC
Complete GO annotation...

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-33543-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Haze protective factor 1
Gene namesi
Name:HPF1
Ordered Locus Names:YOL155C
ORF Names:AOF1001
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL155c.
SGDiS000005515. HPF1.

Subcellular locationi

Secretedcell wall. Membrane; Lipid-anchorGPI-anchor
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP), enriched at bud sites and their neighborhood.3 Publications

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular region Source: SGD
  3. fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

Pathology & Biotechi

Biotechnological usei

Probable mannoprotein called haze protective factor from wine that is able to prevent visible wine protein haze formation. This mannoprotein showed haze-protective activity against wine proteins and BSA when either was heated in white wine.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed prediction
Add
BLAST
Chaini24 – 946923Haze protective factor 1
PRO_0000268177Add
BLAST
Propeptidei947 – 96721Removed in mature form Reviewed prediction
PRO_0000268178Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi28 – 281N-linked (GlcNAc...) Reviewed prediction
Glycosylationi35 – 351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi493 – 4931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi601 – 6011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi638 – 6381N-linked (GlcNAc...) Reviewed prediction
Lipidationi946 – 9461GPI-anchor amidated alanine Reviewed prediction

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PeptideAtlasiQ05164.

Expressioni

Gene expression databases

GenevestigatoriQ05164.

Interactioni

Protein-protein interaction databases

BioGridi34263. 27 interactions.
MINTiMINT-4806805.

Structurei

3D structure databases

ProteinModelPortaliQ05164.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati93 – 105131-1
Add
BLAST
Repeati106 – 118131-2
Add
BLAST
Repeati119 – 131131-3
Add
BLAST
Repeati132 – 144131-4
Add
BLAST
Repeati153 – 165131-5
Add
BLAST
Repeati166 – 178131-6
Add
BLAST
Repeati179 – 191131-7
Add
BLAST
Repeati192 – 204131-8
Add
BLAST
Repeati205 – 217131-9
Add
BLAST
Repeati218 – 230131-10; approximate
Add
BLAST
Repeati234 – 247141-11; approximate
Add
BLAST
Repeati248 – 259121-12; approximate
Add
BLAST
Repeati266 – 278131-13
Add
BLAST
Repeati745 – 780362-1
Add
BLAST
Repeati781 – 815352-2
Add
BLAST
Repeati816 – 854392-3
Add
BLAST
Repeati855 – 893392-4
Add
BLAST
Repeati894 – 90292-5; truncated

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 27818613 X approximate repeats, Ser-rich
Add
BLAST
Regioni745 – 9021584.5 X approximate tandem repeats, Thr-rich
Add
BLAST

Domaini

The number of the intragenic tandem repeats varies between different S.cerevisiae strains.

Sequence similaritiesi

Belongs to the SRP1/TIP1 family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00730000112313.
OMAiWSIPTCV.
OrthoDBiEOG7VQJPN.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05164-1 [UniParc]FASTAAdd to Basket

« Hide

MFNRFNKLQA ALALVLYSQS ALGQYYTNSS SIASNSSTAV SSTSSGSVSI    50
SSSIELTSST SDVSSSLTEL TSSSTEVSSS IAPSTSSSEV SSSITSSGSS 100
VSGSSSITSS GSSVSSSSSA TESGSSASGS SSATESGSSV SGSSTSITSG 150
SSSATESGSS VSGSTSATES GSSASGSSSA TESGSSASGS SSATESGSSV 200
SGSSSATESG SSVSGSSSAT ESGSASSVPS SSGSVTESGS SSSASESSIT 250
QSGTASGSSA SSTSGSVTQS GSSVSGSSAS SAPGISSSIP QSTSSASTAS 300
GSITSGTLSS ITSSASSATA TASNSLSSSD GTIYLPSTTI SGDITLTGSV 350
IATEAVEVAA GGKLTLLDGD KYVFSADFII HGGVFVEKSK PTYPGTEFDI 400
SGENFDVSGT FNAEEPAASS ASAYSFTPGS FDNSGDISLS LSESTKGEVT 450
FSPYSNSGAF SFSNAILNGG SVSGLQRRAE SGSVNNGEIN LENGSTYVVV 500
EPVSGSGTIN IISGNLYLHY PDTFTGQTVV FKGEGVLAVD PTETNTTPIP 550
VVGYTGENQI AITADVTALS YDSATGVLTA TQGNSQFSFS IGTGFSSSGF 600
NVSEGTFAGA YAYYLNYGGV VASSATPSST STTSGATNST SGSTSFGASV 650
TGSTASTSFG ASVTGSTAST LISGSPSVYT TTLTYATTTS TVVVSCSETT 700
DSNGNVYTIT TTVPCSSTTA TITSCDETGC HVTTSTGTVA TETVSSKSYT 750
TVTVTHCDNN GCNTKTVTSE CPEETSATTT SPKSYTTVTV THCDDNGCNT 800
KTVTSEAPEA TTTTVSPKTY TTATVTQCDD NGCSTKTVTS EAPKETSETS 850
ETSAAPKTYT TATVTQCDDN GCNVKIITSQ IPEATSTVTA TSASPKSYTT 900
VTSEGSKATS LTTAISKASS AISTYSKSAA PIKTSTGIIV QSEGIAAGLN 950
ANTLNALVGI FVLAFFN 967
Length:967
Mass (Da):94,706
Last modified:December 12, 2006 - v2
Checksum:i7BFC01EA243A561E
GO

Sequence cautioni

The sequence CAA61860.1 differs from that shown. Reason: Erroneous sequence assembling in the Ser-rich region leading to a longer sequence.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89715 Genomic DNA. Translation: CAA61860.1. Sequence problems.
Z74897 Genomic DNA. Translation: CAA99177.1.
BK006948 Genomic DNA. Translation: DAA10632.1.
PIRiS66852.
RefSeqiNP_014487.1. NM_001183408.1.

Genome annotation databases

EnsemblFungiiYOL155C; YOL155C; YOL155C.
GeneIDi854010.
KEGGisce:YOL155C.

Cross-referencesi

Web resourcesi

Protein Spotlight

Of froth and haze - Issue 78 of January 2007

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X89715 Genomic DNA. Translation: CAA61860.1 . Sequence problems.
Z74897 Genomic DNA. Translation: CAA99177.1 .
BK006948 Genomic DNA. Translation: DAA10632.1 .
PIRi S66852.
RefSeqi NP_014487.1. NM_001183408.1.

3D structure databases

ProteinModelPortali Q05164.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34263. 27 interactions.
MINTi MINT-4806805.

Proteomic databases

PeptideAtlasi Q05164.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL155C ; YOL155C ; YOL155C .
GeneIDi 854010.
KEGGi sce:YOL155C.

Organism-specific databases

CYGDi YOL155c.
SGDi S000005515. HPF1.

Phylogenomic databases

GeneTreei ENSGT00730000112313.
OMAi WSIPTCV.
OrthoDBi EOG7VQJPN.

Enzyme and pathway databases

BioCyci YEAST:G3O-33543-MONOMER.

Miscellaneous databases

NextBioi 975524.

Gene expression databases

Genevestigatori Q05164.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the DNA sequence of a 15,500 bp fragment near the left telomere of chromosome XV from Saccharomyces cerevisiae reveals a putative sugar transporter, a carboxypeptidase homologue and two new open reading frames."
    Gamo F.-J., Lafuente M.J., Casamayor A., Arino J., Aldea M., Casas C., Herrero E., Gancedo C.
    Yeast 12:709-714(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Reducing haziness in white wine by overexpression of Saccharomyces cerevisiae genes YOL155c and YDR055w."
    Brown S.L., Stockdale V.J., Pettolino F., Pocock K.F., de Barros Lopes M., Williams P.J., Bacic A., Fincher G.B., Hoej P.B., Waters E.J.
    Appl. Microbiol. Biotechnol. 73:1363-1376(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 479-504, FUNCTION, BIOTECHNOLOGY.
  5. "Saccharomyces cerevisiae mannoproteins that protect wine from protein haze: evaluation of extraction methods and immunolocalization."
    Dupin I.V.S., Stockdale V.J., Williams P.J., Jones G.P., Markides A.J., Waters E.J.
    J. Agric. Food Chem. 48:1086-1095(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOTECHNOLOGY.
  6. "Cooperative functions of the mannoprotein-encoding genes in the biogenesis and maintenance of the cell wall in Saccharomyces cerevisiae."
    Horie T., Isono K.
    Yeast 18:1493-1503(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. "Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae."
    Terashima H., Fukuchi S., Nakai K., Arisawa M., Hamada K., Yabuki N., Kitada K.
    Curr. Genet. 40:311-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  8. "Intragenic tandem repeats generate functional variability."
    Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
    Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPEATS.

Entry informationi

Entry nameiHPF1_YEAST
AccessioniPrimary (citable) accession number: Q05164
Secondary accession number(s): D6W1R6, Q08294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 12, 2006
Last modified: May 14, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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