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Q05164

- HPF1_YEAST

UniProt

Q05164 - HPF1_YEAST

Protein

Haze protective factor 1

Gene

HPF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (12 Dec 2006)
      Previous versions | rss
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    Functioni

    Involved in cell wall organization and biosynthesis.3 Publications

    GO - Molecular functioni

    1. glucosidase activity Source: SGD

    GO - Biological processi

    1. fungal-type cell wall organization Source: SGD
    2. metabolic process Source: GOC

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33543-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Haze protective factor 1
    Gene namesi
    Name:HPF1
    Ordered Locus Names:YOL155C
    ORF Names:AOF1001
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL155c.
    SGDiS000005515. HPF1.

    Subcellular locationi

    Secretedcell wall. Membrane; Lipid-anchorGPI-anchor
    Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP), enriched at bud sites and their neighborhood.

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: SGD
    3. fungal-type cell wall Source: SGD

    Keywords - Cellular componenti

    Cell wall, Membrane, Secreted

    Pathology & Biotechi

    Biotechnological usei

    Probable mannoprotein called haze protective factor from wine that is able to prevent visible wine protein haze formation. This mannoprotein showed haze-protective activity against wine proteins and BSA when either was heated in white wine.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 946923Haze protective factor 1PRO_0000268177Add
    BLAST
    Propeptidei947 – 96721Removed in mature formSequence AnalysisPRO_0000268178Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi28 – 281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi493 – 4931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi601 – 6011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi638 – 6381N-linked (GlcNAc...)Sequence Analysis
    Lipidationi946 – 9461GPI-anchor amidated alanineSequence Analysis

    Post-translational modificationi

    The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PeptideAtlasiQ05164.

    Expressioni

    Gene expression databases

    GenevestigatoriQ05164.

    Interactioni

    Protein-protein interaction databases

    BioGridi34263. 27 interactions.
    MINTiMINT-4806805.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05164.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati93 – 105131-11 PublicationAdd
    BLAST
    Repeati106 – 118131-21 PublicationAdd
    BLAST
    Repeati119 – 131131-31 PublicationAdd
    BLAST
    Repeati132 – 144131-41 PublicationAdd
    BLAST
    Repeati153 – 165131-51 PublicationAdd
    BLAST
    Repeati166 – 178131-61 PublicationAdd
    BLAST
    Repeati179 – 191131-71 PublicationAdd
    BLAST
    Repeati192 – 204131-81 PublicationAdd
    BLAST
    Repeati205 – 217131-91 PublicationAdd
    BLAST
    Repeati218 – 230131-10; approximate1 PublicationAdd
    BLAST
    Repeati234 – 247141-11; approximate1 PublicationAdd
    BLAST
    Repeati248 – 259121-12; approximate1 PublicationAdd
    BLAST
    Repeati266 – 278131-131 PublicationAdd
    BLAST
    Repeati745 – 780362-11 PublicationAdd
    BLAST
    Repeati781 – 815352-21 PublicationAdd
    BLAST
    Repeati816 – 854392-31 PublicationAdd
    BLAST
    Repeati855 – 893392-41 PublicationAdd
    BLAST
    Repeati894 – 90292-5; truncated1 Publication

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni93 – 27818613 X approximate repeats, Ser-richAdd
    BLAST
    Regioni745 – 9021584.5 X approximate tandem repeats, Thr-richAdd
    BLAST

    Domaini

    The number of the intragenic tandem repeats varies between different S.cerevisiae strains.

    Sequence similaritiesi

    Belongs to the SRP1/TIP1 family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    GeneTreeiENSGT00730000112313.
    OMAiWSIPTCV.
    OrthoDBiEOG7VQJPN.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05164-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFNRFNKLQA ALALVLYSQS ALGQYYTNSS SIASNSSTAV SSTSSGSVSI    50
    SSSIELTSST SDVSSSLTEL TSSSTEVSSS IAPSTSSSEV SSSITSSGSS 100
    VSGSSSITSS GSSVSSSSSA TESGSSASGS SSATESGSSV SGSSTSITSG 150
    SSSATESGSS VSGSTSATES GSSASGSSSA TESGSSASGS SSATESGSSV 200
    SGSSSATESG SSVSGSSSAT ESGSASSVPS SSGSVTESGS SSSASESSIT 250
    QSGTASGSSA SSTSGSVTQS GSSVSGSSAS SAPGISSSIP QSTSSASTAS 300
    GSITSGTLSS ITSSASSATA TASNSLSSSD GTIYLPSTTI SGDITLTGSV 350
    IATEAVEVAA GGKLTLLDGD KYVFSADFII HGGVFVEKSK PTYPGTEFDI 400
    SGENFDVSGT FNAEEPAASS ASAYSFTPGS FDNSGDISLS LSESTKGEVT 450
    FSPYSNSGAF SFSNAILNGG SVSGLQRRAE SGSVNNGEIN LENGSTYVVV 500
    EPVSGSGTIN IISGNLYLHY PDTFTGQTVV FKGEGVLAVD PTETNTTPIP 550
    VVGYTGENQI AITADVTALS YDSATGVLTA TQGNSQFSFS IGTGFSSSGF 600
    NVSEGTFAGA YAYYLNYGGV VASSATPSST STTSGATNST SGSTSFGASV 650
    TGSTASTSFG ASVTGSTAST LISGSPSVYT TTLTYATTTS TVVVSCSETT 700
    DSNGNVYTIT TTVPCSSTTA TITSCDETGC HVTTSTGTVA TETVSSKSYT 750
    TVTVTHCDNN GCNTKTVTSE CPEETSATTT SPKSYTTVTV THCDDNGCNT 800
    KTVTSEAPEA TTTTVSPKTY TTATVTQCDD NGCSTKTVTS EAPKETSETS 850
    ETSAAPKTYT TATVTQCDDN GCNVKIITSQ IPEATSTVTA TSASPKSYTT 900
    VTSEGSKATS LTTAISKASS AISTYSKSAA PIKTSTGIIV QSEGIAAGLN 950
    ANTLNALVGI FVLAFFN 967
    Length:967
    Mass (Da):94,706
    Last modified:December 12, 2006 - v2
    Checksum:i7BFC01EA243A561E
    GO

    Sequence cautioni

    The sequence CAA61860.1 differs from that shown. Reason: Erroneous sequence assembling in the Ser-rich region leading to a longer sequence.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89715 Genomic DNA. Translation: CAA61860.1. Sequence problems.
    Z74897 Genomic DNA. Translation: CAA99177.1.
    BK006948 Genomic DNA. Translation: DAA10632.1.
    PIRiS66852.
    RefSeqiNP_014487.1. NM_001183408.1.

    Genome annotation databases

    EnsemblFungiiYOL155C; YOL155C; YOL155C.
    GeneIDi854010.
    KEGGisce:YOL155C.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Of froth and haze - Issue 78 of January 2007

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X89715 Genomic DNA. Translation: CAA61860.1 . Sequence problems.
    Z74897 Genomic DNA. Translation: CAA99177.1 .
    BK006948 Genomic DNA. Translation: DAA10632.1 .
    PIRi S66852.
    RefSeqi NP_014487.1. NM_001183408.1.

    3D structure databases

    ProteinModelPortali Q05164.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34263. 27 interactions.
    MINTi MINT-4806805.

    Proteomic databases

    PeptideAtlasi Q05164.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL155C ; YOL155C ; YOL155C .
    GeneIDi 854010.
    KEGGi sce:YOL155C.

    Organism-specific databases

    CYGDi YOL155c.
    SGDi S000005515. HPF1.

    Phylogenomic databases

    GeneTreei ENSGT00730000112313.
    OMAi WSIPTCV.
    OrthoDBi EOG7VQJPN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33543-MONOMER.

    Miscellaneous databases

    NextBioi 975524.

    Gene expression databases

    Genevestigatori Q05164.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the DNA sequence of a 15,500 bp fragment near the left telomere of chromosome XV from Saccharomyces cerevisiae reveals a putative sugar transporter, a carboxypeptidase homologue and two new open reading frames."
      Gamo F.-J., Lafuente M.J., Casamayor A., Arino J., Aldea M., Casas C., Herrero E., Gancedo C.
      Yeast 12:709-714(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Reducing haziness in white wine by overexpression of Saccharomyces cerevisiae genes YOL155c and YDR055w."
      Brown S.L., Stockdale V.J., Pettolino F., Pocock K.F., de Barros Lopes M., Williams P.J., Bacic A., Fincher G.B., Hoej P.B., Waters E.J.
      Appl. Microbiol. Biotechnol. 73:1363-1376(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 479-504, FUNCTION, BIOTECHNOLOGY.
    5. "Saccharomyces cerevisiae mannoproteins that protect wine from protein haze: evaluation of extraction methods and immunolocalization."
      Dupin I.V.S., Stockdale V.J., Williams P.J., Jones G.P., Markides A.J., Waters E.J.
      J. Agric. Food Chem. 48:1086-1095(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, BIOTECHNOLOGY.
    6. "Cooperative functions of the mannoprotein-encoding genes in the biogenesis and maintenance of the cell wall in Saccharomyces cerevisiae."
      Horie T., Isono K.
      Yeast 18:1493-1503(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    7. "Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae."
      Terashima H., Fukuchi S., Nakai K., Arisawa M., Hamada K., Yabuki N., Kitada K.
      Curr. Genet. 40:311-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "Intragenic tandem repeats generate functional variability."
      Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
      Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REPEATS.

    Entry informationi

    Entry nameiHPF1_YEAST
    AccessioniPrimary (citable) accession number: Q05164
    Secondary accession number(s): D6W1R6, Q08294
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: December 12, 2006
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3