Q05159 (EGR2B_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Early growth response protein 2b
Short name=EGR-2b

Alternative name(s):
Protein krx-20
Zinc finger protein Krox-20

Gene names

Name:	egr2b
Synonyms:	egr2, krx-20, krx20

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sequence-specific DNA-binding transcription factor. Binds to two specific DNA sites located in the promoter region of HOXA4.
Subcellular location	Nucleus.
Developmental stage	First detected during late gastrulation, found in the prospective neuroepithelium domain around 100% epiboly and begins to constrict along the neuroaxis by the end of gastrulation - around 10 hours post-fertilization (hpf). By 12 hpf expression is restricted in two regions of the neuroepithelium of the prospective hindbrain; rhombomeres 3 and 5. Around 14 hpf neural crest migration begins from the dorsal surface of R5 moving caudally into R6 and then ventrally towards the pharyngeal arches. Crest migration is not apparent at or after 16 hpf and no migration is seen from R3. Expression is down-regulated first in R3 around 26 hpf and later in R5 around 30 hpf.
Sequence similarities	Belongs to the EGR C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	myelination of posterior lateral line nerve axons Inferred from mutant phenotype. Source: ZFIN regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW rhombomere boundary formation Inferred from mutant phenotype. Source: ZFIN transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Early growth response protein 2b		PRO_0000047124
Regions
	Zinc finger	299 – 323	25	C2H2-type 1
	Zinc finger	329 – 351	23	C2H2-type 2
	Zinc finger	357 – 379	23	C2H2-type 3

Sequences

	Sequence		Length	Mass (Da)
	Q05159 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 8DC42A9A0A400291	FASTA	412	45,153
	10 20 30 40 50 60 MTAKTLEKAP VSLGGFVHPL ADSIYSVDEL GTTLPASVTI YNDLGGHYEQ INAGDGLING 70 80 90 100 110 120 DMSTEKRALD LAYSSTFAQP AGPRNQTFTY MGKFSIDSQY PGNWNPEGVI NIVSAGILGM 130 140 150 160 170 180 TQPSSASSSP ASSVSPSHFS STLSCTMAQN QADMEHIYSP PPPYSGCGEV YQDPSAFLST 190 200 210 220 230 240 STCPISYPPP SYSSPKPNAD SGLFPIIPDY AGFFQPPCQR DMQSMPDRKP FSCPLDSFKL 250 260 270 280 290 300 PPPLTPLNTI RNFTLGGPGP DGPRLPTAYT PQNLPLRPIL RPRKYPNRPS KTPVHERPYP 310 320 330 340 350 360 CPAEGCDRRF SRSDELTRHI RIHTGHKPFQ CRICMRNFSR SDHLTTHIRT HTGEKPFACD 370 380 390 400 410 FCGRKFARSD ERKRHTKIHL RQKERKSSSS STGVSSSERG VATSICSSSS NQ

References

[1]	"Cloning of the zebrafish krox-20 gene (krx-20) and its expression during hindbrain development." Oxtoby E., Jowett T. Nucleic Acids Res. 21:1087-1095(1993) [PubMed: 8464695] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X70322 mRNA. Translation: CAA49790.1.
IPI	IPI00484958.
PIR	S30299.
UniGene	Dr.75080.
3D structure databases
ProteinModelPortal	Q05159.
SMR	Q05159. Positions 296-382.
ModBase	Search...
Protein-protein interaction databases
STRING	Q05159.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
ZFIN	ZDB-GENE-980526-283. egr2b.
Phylogenomic databases
eggNOG	fiNOG04973.
HOGENOM	HBG717852.
HOVERGEN	HBG003909.
InParanoid	Q05159.
OrthoDB	EOG41NTMJ.
Gene expression databases
ArrayExpress	Q05159.
Bgee	Q05159.
Family and domain databases
InterPro	IPR021849. DUF3446. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.30.160.60. Znf_C2H2/integrase_DNA-bd. 3 hits.
Pfam	PF11928. DUF3446. 1 hit. PF00096. zf-C2H2. 3 hits. [Graphical view]
SMART	SM00355. ZnF_C2H2. 3 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 3 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EGR2B_DANRE

Accession

Primary (citable) accession number: Q05159

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	October 19, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program