Q05156 (GUN1_STRRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cellulase 1
EC=3.2.1.4

Alternative name(s):
Avicelase
Endo-1,4-beta-glucanase
Endoglucanase

Gene names

Name:

cel1

Organism

Streptomyces reticuli

Taxonomic identifier

1926 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	746 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This endoglucanase acts only on crystalline cellulose.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Note: Exists in both mycelium-associated and extracellular forms.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family. Contains 1 CBM-cenC (cenC-type cellulose-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Ref.1
	Chain	30 – 746	717	Cellulase 1		PRO_0000007958
Regions
	Domain	45 – 170	126	CBM-cenC
	Region	171 – 211	41	Linker
	Region	212 – 746	535	Catalytic
Sites
	Active site	669	1	By similarity
	Active site	717	1	By similarity
	Active site	726	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05156 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: FDF1ADB4714A35FD	FASTA	746	80,000
	10 20 30 40 50 60 MKRRTTAVLT LTALLGTALT ALPVQQAGAE EVEQVRNGTF DTTTDPWWTS NVTAGLSDGR 70 80 90 100 110 120 LCADVPGGTT NRWDSAIGQN DITLVKGETY RFSFHASGIP EGHVVRAVVG LAVSPYDTWQ 130 140 150 160 170 180 EASPVLTEAD GSYSYTFTAP VDTTQGQVAF QVGGSTDAWR FCVDDVSLLG GVPPEVYEPD 190 200 210 220 230 240 TGPRVRVNQV AYLPAGPKNA TLVTDATARL PWQLRNAQGT TVARGLTVPR GVDASSGQNV 250 260 270 280 290 300 HSIDFGSYRG RGTGYTLVAD GETSHPFDID AAAYRPLRLD SVKYYYTQRS GIAIRDDLRP 310 320 330 340 350 360 GYGRAAGHLN VAPNQGDANV PCQPGVCDYT LDVTGGWYDA GDHGKYVVNG GIATWELLST 370 380 390 400 410 420 YERSLTARTG HPAALGDGTL ALPESGNKVP DVLDEARWEL EFLLKMQVPA GQPLAGMAHH 430 440 450 460 470 480 KLHDEQWTGL PLLPDQDPQK RELHPPTTAA TLNLAATAAQ AARLYRPFDK AFAARALTAA 490 500 510 520 530 540 RTAWQAALAH PDLLADPNDG TGGGAYNDDD VTDEFYWAAA ELYLTTGERQ FADHVLDSPV 550 560 570 580 590 600 HTADIFGPTG FDWGHTAAAG RLDLALVPSR LPGRDQVRRS VIKAADTYLA TLTAHPYGMP 610 620 630 640 650 660 YAPAGNRYDW GSSHQVLNNG VVLASAYDLT GAAKYRDGAL QGMDYVLGRN ALNMSYVTGY 670 680 690 700 710 720 GEVSSHNQHS RWYAHQLDPT LPNPPSGTLA GGPNSSIQDP YAQSKLTGCV GQFCYIDDIQ 730 740 SWSTNETAIN WNAALARMAS FAADQG

References

[1]

"The gene encoding the cellulase (Avicelase) Cel1 from Streptomyces reticuli and analysis of protein domains." Schlochtermeier A., Walter S., Schroeder J., Moorman M., Schrempf H. Mol. Microbiol. 6:3611-3621(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-41 AND 379-391. Strain: Tu45.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X65616 Genomic DNA. Translation: CAA46570.1.
PIR	S27985.
3D structure databases
ProteinModelPortal	Q05156.
ModBase	Search...
Protein family/group databases
CAZy	CBM4. Carbohydrate-Binding Module Family 4. GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.50.10.10. 1 hit. 2.60.120.260. 1 hit. 2.60.40.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR003305. CenC_carb-bd. IPR008979. Galactose-bd-like. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. IPR004197. Glyco_hydro_9_Ig-like. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Pfam	PF02018. CBM_4_9. 1 hit. PF02927. CelD_N. 1 hit. PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF48208. Glyco_trans_6hp. 1 hit. SSF81296. Ig_E-set. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN1_STRRE

Accession

Primary (citable) accession number: Q05156

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 29, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program