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Reviewed, UniProtKB/Swiss-Prot Q05156 (GUN1_STRRE)

Last modified February 9, 2010. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cellulase 1
    EC=3.2.1.4
Alternative name(s):
    Endoglucanase
    Endo-1,4-beta-glucanase
    Avicelase
Gene names
Name: cel1
OrganismStreptomyces reticuli
Taxonomic identifier1926 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length746 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

This endoglucanase acts only on crystalline cellulose.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Note: Exists in both mycelium-associated and extracellular forms.

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Contains 1 CBM-cenC (cenC-type cellulose-binding) domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functioncellulase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Ref.1
Chain30 – 746717Cellulase 1
PRO_0000007958

Regions

Domain45 – 170126CBM-cenC
Region171 – 21141Linker
Region212 – 746535Catalytic

Sites

Active site6691 By similarity
Active site7171 By similarity
Active site7261 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05156-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: FDF1ADB4714A35FD

FASTA74680,000
        10         20         30         40         50         60 
MKRRTTAVLT LTALLGTALT ALPVQQAGAE EVEQVRNGTF DTTTDPWWTS NVTAGLSDGR 

        70         80         90        100        110        120 
LCADVPGGTT NRWDSAIGQN DITLVKGETY RFSFHASGIP EGHVVRAVVG LAVSPYDTWQ 

       130        140        150        160        170        180 
EASPVLTEAD GSYSYTFTAP VDTTQGQVAF QVGGSTDAWR FCVDDVSLLG GVPPEVYEPD 

       190        200        210        220        230        240 
TGPRVRVNQV AYLPAGPKNA TLVTDATARL PWQLRNAQGT TVARGLTVPR GVDASSGQNV 

       250        260        270        280        290        300 
HSIDFGSYRG RGTGYTLVAD GETSHPFDID AAAYRPLRLD SVKYYYTQRS GIAIRDDLRP 

       310        320        330        340        350        360 
GYGRAAGHLN VAPNQGDANV PCQPGVCDYT LDVTGGWYDA GDHGKYVVNG GIATWELLST 

       370        380        390        400        410        420 
YERSLTARTG HPAALGDGTL ALPESGNKVP DVLDEARWEL EFLLKMQVPA GQPLAGMAHH 

       430        440        450        460        470        480 
KLHDEQWTGL PLLPDQDPQK RELHPPTTAA TLNLAATAAQ AARLYRPFDK AFAARALTAA 

       490        500        510        520        530        540 
RTAWQAALAH PDLLADPNDG TGGGAYNDDD VTDEFYWAAA ELYLTTGERQ FADHVLDSPV 

       550        560        570        580        590        600 
HTADIFGPTG FDWGHTAAAG RLDLALVPSR LPGRDQVRRS VIKAADTYLA TLTAHPYGMP 

       610        620        630        640        650        660 
YAPAGNRYDW GSSHQVLNNG VVLASAYDLT GAAKYRDGAL QGMDYVLGRN ALNMSYVTGY 

       670        680        690        700        710        720 
GEVSSHNQHS RWYAHQLDPT LPNPPSGTLA GGPNSSIQDP YAQSKLTGCV GQFCYIDDIQ 

       730        740 
SWSTNETAIN WNAALARMAS FAADQG 

« Hide

References

[1]"The gene encoding the cellulase (Avicelase) Cel1 from Streptomyces reticuli and analysis of protein domains."
Schlochtermeier A., Walter S., Schroeder J., Moorman M., Schrempf H.
Mol. Microbiol. 6:3611-3621(1992) [PubMed: 1282194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-41 AND 379-391.
Strain: Tu45.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X65616 Genomic DNA. Translation: CAA46570.1.
PIRS27985.

3D structure databases

SMRQ05156. Positions 39-170, 183-745.
ModBaseSearch...

Protein family/group databases

CAZyCBM4. Carbohydrate-Binding Module Family 4.
GH9. Glycoside Hydrolase Family 9.

Enzyme and pathway databases

BRENDA3.2.1.4. 257915.

Family and domain databases

InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR003305. CenC_carb_bd.
IPR008979. Galactose-bd-like.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
Gene3DG3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHERPTHR22298:SF3. Glyco_hydro_9. 1 hit.
PfamPF02018. CBM_4_9. 1 hit.
PF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
PROSITEPS00592. GLYCOSYL_HYDROL_F9_1. False negative.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN1_STRRE
AccessionPrimary (citable) accession number: Q05156
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 9, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents