ID   MDHP_MESCR              Reviewed;         441 AA.
AC   Q05145;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   16-JUN-2009, entry version 72.
DE   RecName: Full=Malate dehydrogenase [NADP], chloroplastic;
DE            EC=1.1.1.82;
DE   AltName: Full=NADP-MDH;
DE   Flags: Precursor;
GN   Name=MDH1;
OS   Mesembryanthemum crystallinum (Common ice plant).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   Caryophyllales; Aizoaceae; Mesembryanthemum.
OX   NCBI_TaxID=3544;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf, and Root;
RA   Cushman J.C.;
RT   "Molecular cloning and expression of chloroplast NADP-malate
RT   dehydrogenase during Crassulacean acid metabolism induction by salt
RT   stress.";
RL   Photosyn. Res. 35:15-27(1993).
CC   -!- FUNCTION: The chloroplastic, NADP-dependent form is essential for
CC       the photosynthesis C4 cycle, which allows plants to circumvent the
CC       problem of photorespiration. In C4 plants, NADP-MDH activity acts
CC       to convert oxaloacetate to malate in chloroplasts of mesophyll
CC       cells for transport to the bundle sheath cells.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NADP(+) = oxaloacetate + NADPH.
CC   -!- ENZYME REGULATION: Chloroplast NADP-MDH is activated upon
CC       illumination. In order to be enzymatically active, disulfides
CC       bridges on the protein must be reduced by thioredoxin which
CC       receives electrons from ferredoxin and the electron transport
CC       system of photosynthesis.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- INDUCTION: Expression decreases transiently in the leaves after
CC       salt stress and then increases to levels greater than two-fold
CC       higher than in unstressed plants.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 2 family.
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DR   EMBL; X63727; CAA45270.1; -; mRNA.
DR   PIR; S33066; S33066.
DR   HSSP; P46489; 1CIV.
DR   SMR; Q05145; 72-438.
DR   BRENDA; 1.1.1.82; 2210.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0046554; F:malate dehydrogenase (NADP+) activity; IEA:EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001236; Lactate/malate_DH.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR011273; Malate_DH_NADP-dep_pln.
DR   InterPro; IPR010945; Malate_DH_SF1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR23382; MDH_SF1; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   ProDom; PD003052; Mal_dehydrog; 1.
DR   TIGRFAMs; TIGR01757; Malate-DH_plant; 1.
DR   TIGRFAMs; TIGR01759; MalateDH-SF1; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Disulfide bond; NADP; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT       1     51       Chloroplast (Potential).
FT   CHAIN        52    441       Malate dehydrogenase [NADP],
FT                                chloroplastic.
FT                                /FTId=PRO_0000018645.
FT   NP_BIND     104    110       NADP (By similarity).
FT   NP_BIND     222    224       NADP (By similarity).
FT   ACT_SITE    280    280       Proton acceptor (By similarity).
FT   BINDING     185    185       Substrate (By similarity).
FT   BINDING     191    191       Substrate (By similarity).
FT   BINDING     198    198       NADP (By similarity).
FT   BINDING     205    205       NAD (By similarity).
FT   BINDING     224    224       Substrate (By similarity).
FT   BINDING     255    255       Substrate (By similarity).
FT   SITE         75     75       Activation of NADP-MDH (By similarity).
FT   SITE         80     80       Activation of NADP-MDH (By similarity).
FT   DISULFID     75     80       In oxidized inactive NAD-MDH (By
FT                                similarity).
FT   DISULFID    416    428       In oxidized inactive NAD-MDH (By
FT                                similarity).
SQ   SEQUENCE   441 AA;  48004 MW;  77AFB169F1488FA7 CRC64;
     MAVAELSPSY KTQLKTCQQL SSSLSTRLSD HRKFSLRLLP RPVSVRGGIR CSVAPNQVQA
     PVAVPAEGQT GKPECYGIFC LTYDLKAEEE TKTWKKMITI AVSGAAGMIS NHLLFKLASG
     EVFGPDQPIA LKLLGSERSF NALEGVAMEL EDSLYPLLRA VSIGIDPYDI FQDAEWALLI
     GAKPRGPGME RADLLDINGQ IFAEQGKALN AVASRNVKVI VVGNPCNTNA LICLKNAPNI
     PAKNFHGLTR LDENRAKCQL ALKAGVFYDK VSNMTIWGNH STTQVPDFLN AKIDGLPVKT
     VIKDHKWLEE EFTVMIQKRG GALIQKWGRS SAASTAVSIA DAIKSLVTPT PEGDWFSSAV
     YTNGNPYGIA EDLVFSMPCR SKGDGDYELV KDVVFDDYLR QRIKKSEEEL LAEKRCTAHL
     TGEGVAVCDL PAGDTMLPGE M
//