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Q05145 (MDHP_MESCR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase [NADP], chloroplastic

EC=1.1.1.82
Alternative name(s):
NADP-MDH
Gene names
Name:MDH1
OrganismMesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
Taxonomic identifier3544 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAizoaceaeMesembryanthemum

Protein attributes

Sequence length441 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

Catalytic activity

(S)-malate + NADP+ = oxaloacetate + NADPH.

Enzyme regulation

Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastidchloroplast.

Induction

Expression decreases transiently in the leaves after salt stress and then increases to levels greater than two-fold higher than in unstressed plants.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandNADP
   Molecular functionOxidoreductase
   PTMDisulfide bond
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate dehydrogenase (NADP+) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5151Chloroplast Potential
Chain52 – 441390Malate dehydrogenase [NADP], chloroplastic
PRO_0000018645

Regions

Nucleotide binding104 – 1107NADP By similarity
Nucleotide binding222 – 2243NADP By similarity

Sites

Active site2801Proton acceptor By similarity
Binding site1851Substrate By similarity
Binding site1911Substrate By similarity
Binding site1981NADP By similarity
Binding site2051NAD By similarity
Binding site2241Substrate By similarity
Binding site2551Substrate By similarity
Site751Activation of NADP-MDH By similarity
Site801Activation of NADP-MDH By similarity

Amino acid modifications

Disulfide bond75 ↔ 80In oxidized inactive NAD-MDH By similarity
Disulfide bond416 ↔ 428In oxidized inactive NAD-MDH By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05145 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 77AFB169F1488FA7

FASTA44148,004
        10         20         30         40         50         60 
MAVAELSPSY KTQLKTCQQL SSSLSTRLSD HRKFSLRLLP RPVSVRGGIR CSVAPNQVQA 

        70         80         90        100        110        120 
PVAVPAEGQT GKPECYGIFC LTYDLKAEEE TKTWKKMITI AVSGAAGMIS NHLLFKLASG 

       130        140        150        160        170        180 
EVFGPDQPIA LKLLGSERSF NALEGVAMEL EDSLYPLLRA VSIGIDPYDI FQDAEWALLI 

       190        200        210        220        230        240 
GAKPRGPGME RADLLDINGQ IFAEQGKALN AVASRNVKVI VVGNPCNTNA LICLKNAPNI 

       250        260        270        280        290        300 
PAKNFHGLTR LDENRAKCQL ALKAGVFYDK VSNMTIWGNH STTQVPDFLN AKIDGLPVKT 

       310        320        330        340        350        360 
VIKDHKWLEE EFTVMIQKRG GALIQKWGRS SAASTAVSIA DAIKSLVTPT PEGDWFSSAV 

       370        380        390        400        410        420 
YTNGNPYGIA EDLVFSMPCR SKGDGDYELV KDVVFDDYLR QRIKKSEEEL LAEKRCTAHL 

       430        440 
TGEGVAVCDL PAGDTMLPGE M 

« Hide

References

[1]"Molecular cloning and expression of chloroplast NADP-malate dehydrogenase during Crassulacean acid metabolism induction by salt stress."
Cushman J.C.
Photosyn. Res. 35:15-27(1993)
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leaf and Root.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X63727 mRNA. Translation: CAA45270.1.
PIRS33066.

3D structure databases

ProteinModelPortalQ05145.
SMRQ05145. Positions 72-441.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ05145.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDHP_MESCR
AccessionPrimary (citable) accession number: Q05145
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 19, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families