Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q05145

- MDHP_MESCR

UniProt

Q05145 - MDHP_MESCR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Malate dehydrogenase [NADP], chloroplastic

Gene

MDH1

Organism
Mesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

Catalytic activityi

(S)-malate + NADP+ = oxaloacetate + NADPH.

Enzyme regulationi

Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei75 – 751Activation of NADP-MDHBy similarity
Sitei80 – 801Activation of NADP-MDHBy similarity
Binding sitei185 – 1851SubstratePROSITE-ProRule annotation
Binding sitei191 – 1911SubstratePROSITE-ProRule annotation
Binding sitei198 – 1981NADPBy similarity
Binding sitei205 – 2051NADBy similarity
Binding sitei224 – 2241SubstratePROSITE-ProRule annotation
Binding sitei255 – 2551SubstratePROSITE-ProRule annotation
Active sitei280 – 2801Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi104 – 1107NADPBy similarity
Nucleotide bindingi222 – 2243NADPBy similarity

GO - Molecular functioni

  1. malate dehydrogenase (NADP+) activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase [NADP], chloroplastic (EC:1.1.1.82)
Alternative name(s):
NADP-MDH
Gene namesi
Name:MDH1
OrganismiMesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
Taxonomic identifieri3544 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAizoaceaeMesembryanthemum

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5151ChloroplastSequence AnalysisAdd
BLAST
Chaini52 – 441390Malate dehydrogenase [NADP], chloroplasticPRO_0000018645Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 80In oxidized inactive NAD-MDHBy similarity
Disulfide bondi416 ↔ 428In oxidized inactive NAD-MDHBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ05145.

Expressioni

Inductioni

Expression decreases transiently in the leaves after salt stress and then increases to levels greater than two-fold higher than in unstressed plants.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ05145.
SMRiQ05145. Positions 72-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05145-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVAELSPSY KTQLKTCQQL SSSLSTRLSD HRKFSLRLLP RPVSVRGGIR
60 70 80 90 100
CSVAPNQVQA PVAVPAEGQT GKPECYGIFC LTYDLKAEEE TKTWKKMITI
110 120 130 140 150
AVSGAAGMIS NHLLFKLASG EVFGPDQPIA LKLLGSERSF NALEGVAMEL
160 170 180 190 200
EDSLYPLLRA VSIGIDPYDI FQDAEWALLI GAKPRGPGME RADLLDINGQ
210 220 230 240 250
IFAEQGKALN AVASRNVKVI VVGNPCNTNA LICLKNAPNI PAKNFHGLTR
260 270 280 290 300
LDENRAKCQL ALKAGVFYDK VSNMTIWGNH STTQVPDFLN AKIDGLPVKT
310 320 330 340 350
VIKDHKWLEE EFTVMIQKRG GALIQKWGRS SAASTAVSIA DAIKSLVTPT
360 370 380 390 400
PEGDWFSSAV YTNGNPYGIA EDLVFSMPCR SKGDGDYELV KDVVFDDYLR
410 420 430 440
QRIKKSEEEL LAEKRCTAHL TGEGVAVCDL PAGDTMLPGE M
Length:441
Mass (Da):48,004
Last modified:February 1, 1994 - v1
Checksum:i77AFB169F1488FA7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63727 mRNA. Translation: CAA45270.1.
PIRiS33066.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X63727 mRNA. Translation: CAA45270.1 .
PIRi S33066.

3D structure databases

ProteinModelPortali Q05145.
SMRi Q05145. Positions 72-441.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q05145.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011273. Malate_DH_NADP-dep_pln.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01757. Malate-DH_plant. 1 hit.
TIGR01759. MalateDH-SF1. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of chloroplast NADP-malate dehydrogenase during Crassulacean acid metabolism induction by salt stress."
    Cushman J.C.
    Photosyn. Res. 35:15-27(1993)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf and Root.

Entry informationi

Entry nameiMDHP_MESCR
AccessioniPrimary (citable) accession number: Q05145
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3