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Q05145

- MDHP_MESCR

UniProt

Q05145 - MDHP_MESCR

Protein

Malate dehydrogenase [NADP], chloroplastic

Gene

MDH1

Organism
Mesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    The chloroplastic, NADP-dependent form is essential for the photosynthesis C4 cycle, which allows plants to circumvent the problem of photorespiration. In C4 plants, NADP-MDH activity acts to convert oxaloacetate to malate in chloroplasts of mesophyll cells for transport to the bundle sheath cells.

    Catalytic activityi

    (S)-malate + NADP+ = oxaloacetate + NADPH.

    Enzyme regulationi

    Chloroplast NADP-MDH is activated upon illumination. In order to be enzymatically active, disulfide bridges on the protein must be reduced by thioredoxin which receives electrons from ferredoxin and the electron transport system of photosynthesis.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei75 – 751Activation of NADP-MDHBy similarity
    Sitei80 – 801Activation of NADP-MDHBy similarity
    Binding sitei185 – 1851SubstratePROSITE-ProRule annotation
    Binding sitei191 – 1911SubstratePROSITE-ProRule annotation
    Binding sitei198 – 1981NADPBy similarity
    Binding sitei205 – 2051NADBy similarity
    Binding sitei224 – 2241SubstratePROSITE-ProRule annotation
    Binding sitei255 – 2551SubstratePROSITE-ProRule annotation
    Active sitei280 – 2801Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi104 – 1107NADPBy similarity
    Nucleotide bindingi222 – 2243NADPBy similarity

    GO - Molecular functioni

    1. malate dehydrogenase (NADP+) activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase [NADP], chloroplastic (EC:1.1.1.82)
    Alternative name(s):
    NADP-MDH
    Gene namesi
    Name:MDH1
    OrganismiMesembryanthemum crystallinum (Common ice plant) (Cryophytum crystallinum)
    Taxonomic identifieri3544 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAizoaceaeMesembryanthemum

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5151ChloroplastSequence AnalysisAdd
    BLAST
    Chaini52 – 441390Malate dehydrogenase [NADP], chloroplasticPRO_0000018645Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi75 ↔ 80In oxidized inactive NAD-MDHBy similarity
    Disulfide bondi416 ↔ 428In oxidized inactive NAD-MDHBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiQ05145.

    Expressioni

    Inductioni

    Expression decreases transiently in the leaves after salt stress and then increases to levels greater than two-fold higher than in unstressed plants.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ05145.
    SMRiQ05145. Positions 72-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011273. Malate_DH_NADP-dep_pln.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01757. Malate-DH_plant. 1 hit.
    TIGR01759. MalateDH-SF1. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05145-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVAELSPSY KTQLKTCQQL SSSLSTRLSD HRKFSLRLLP RPVSVRGGIR    50
    CSVAPNQVQA PVAVPAEGQT GKPECYGIFC LTYDLKAEEE TKTWKKMITI 100
    AVSGAAGMIS NHLLFKLASG EVFGPDQPIA LKLLGSERSF NALEGVAMEL 150
    EDSLYPLLRA VSIGIDPYDI FQDAEWALLI GAKPRGPGME RADLLDINGQ 200
    IFAEQGKALN AVASRNVKVI VVGNPCNTNA LICLKNAPNI PAKNFHGLTR 250
    LDENRAKCQL ALKAGVFYDK VSNMTIWGNH STTQVPDFLN AKIDGLPVKT 300
    VIKDHKWLEE EFTVMIQKRG GALIQKWGRS SAASTAVSIA DAIKSLVTPT 350
    PEGDWFSSAV YTNGNPYGIA EDLVFSMPCR SKGDGDYELV KDVVFDDYLR 400
    QRIKKSEEEL LAEKRCTAHL TGEGVAVCDL PAGDTMLPGE M 441
    Length:441
    Mass (Da):48,004
    Last modified:February 1, 1994 - v1
    Checksum:i77AFB169F1488FA7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63727 mRNA. Translation: CAA45270.1.
    PIRiS33066.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X63727 mRNA. Translation: CAA45270.1 .
    PIRi S33066.

    3D structure databases

    ProteinModelPortali Q05145.
    SMRi Q05145. Positions 72-441.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q05145.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011273. Malate_DH_NADP-dep_pln.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01757. Malate-DH_plant. 1 hit.
    TIGR01759. MalateDH-SF1. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of chloroplast NADP-malate dehydrogenase during Crassulacean acid metabolism induction by salt stress."
      Cushman J.C.
      Photosyn. Res. 35:15-27(1993)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Leaf and Root.

    Entry informationi

    Entry nameiMDHP_MESCR
    AccessioniPrimary (citable) accession number: Q05145
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3