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Q05144

- RAC2_MOUSE

UniProt

Q05144 - RAC2_MOUSE

Protein

Ras-related C3 botulinum toxin substrate 2

Gene

Rac2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.

    Enzyme regulationi

    Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 178GTPBy similarity
    Nucleotide bindingi57 – 615GTPBy similarity
    Nucleotide bindingi115 – 1184GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: MGI
    2. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. actin cytoskeleton organization Source: MGI
    2. bone resorption Source: Ensembl
    3. cell projection assembly Source: MGI
    4. chemotaxis Source: MGI
    5. G-protein coupled receptor signaling pathway Source: MGI
    6. GTP catabolic process Source: GOC
    7. positive regulation of cell proliferation Source: MGI
    8. regulation of respiratory burst Source: Ensembl
    9. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_204733. G alpha (12/13) signalling events.
    REACT_210090. Rho GTPase cycle.
    REACT_220092. GPVI-mediated activation cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related C3 botulinum toxin substrate 2
    Alternative name(s):
    Protein EN-7
    p21-Rac2
    Gene namesi
    Name:Rac2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:97846. Rac2.

    Subcellular locationi

    Cytoplasm By similarity. Membrane By similarity; Lipid-anchor By similarity
    Note: Membrane-associated when activated.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. membrane Source: MGI
    3. nuclear envelope Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 189189Ras-related C3 botulinum toxin substrate 2PRO_0000042048Add
    BLAST
    Propeptidei190 – 1923Removed in mature formBy similarityPRO_0000042049

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei147 – 1471N6-acetyllysineBy similarity
    Modified residuei189 – 1891Cysteine methyl esterBy similarity
    Lipidationi189 – 1891S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiQ05144.
    PaxDbiQ05144.
    PRIDEiQ05144.

    PTM databases

    PhosphoSiteiQ05144.

    Expressioni

    Gene expression databases

    BgeeiQ05144.
    CleanExiMM_RAC2.
    GenevestigatoriQ05144.

    Interactioni

    Subunit structurei

    Interacts with DOCK2, which may activate it. Interacts with S100A8 and calprotectin (S100A8/9).By similarity

    Protein-protein interaction databases

    BioGridi202557. 8 interactions.
    IntActiQ05144. 2 interactions.
    MINTiMINT-202545.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05144.
    SMRiQ05144. Positions 1-181.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi32 – 409Effector regionSequence Analysis

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rho family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00640000091266.
    HOGENOMiHOG000233974.
    HOVERGENiHBG009351.
    InParanoidiQ05144.
    KOiK07860.
    OMAiREDRSYL.
    OrthoDBiEOG764747.
    PhylomeDBiQ05144.
    TreeFamiTF101109.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00174. RHO. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51420. RHO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05144-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDSKP    50
    VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASY ENVRAKWFPE 100
    VRHHCPSTPI ILVGTKLDLR DDKDTIEKLK EKKLAPITYP QGLALAKDID 150
    SVKYLECSAL TQRGLKTVFD EAIRAVLCPQ PTRQQKRPCS LL 192
    Length:192
    Mass (Da):21,441
    Last modified:February 1, 1994 - v1
    Checksum:i2A1F1266AB9D7705
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti60 – 601G → V in BAB25109. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53247 mRNA. Translation: CAA37337.1.
    AK007561 mRNA. Translation: BAB25109.1.
    AK144136 mRNA. Translation: BAE25721.1.
    AK171321 mRNA. Translation: BAE42390.1.
    BC005455 mRNA. Translation: AAH05455.1.
    CCDSiCCDS27619.1.
    PIRiA60194.
    RefSeqiNP_033034.1. NM_009008.3.
    UniGeneiMm.1972.

    Genome annotation databases

    EnsembliENSMUST00000043214; ENSMUSP00000036384; ENSMUSG00000033220.
    GeneIDi19354.
    KEGGimmu:19354.
    UCSCiuc007wpp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53247 mRNA. Translation: CAA37337.1 .
    AK007561 mRNA. Translation: BAB25109.1 .
    AK144136 mRNA. Translation: BAE25721.1 .
    AK171321 mRNA. Translation: BAE42390.1 .
    BC005455 mRNA. Translation: AAH05455.1 .
    CCDSi CCDS27619.1.
    PIRi A60194.
    RefSeqi NP_033034.1. NM_009008.3.
    UniGenei Mm.1972.

    3D structure databases

    ProteinModelPortali Q05144.
    SMRi Q05144. Positions 1-181.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202557. 8 interactions.
    IntActi Q05144. 2 interactions.
    MINTi MINT-202545.

    PTM databases

    PhosphoSitei Q05144.

    Proteomic databases

    MaxQBi Q05144.
    PaxDbi Q05144.
    PRIDEi Q05144.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043214 ; ENSMUSP00000036384 ; ENSMUSG00000033220 .
    GeneIDi 19354.
    KEGGi mmu:19354.
    UCSCi uc007wpp.1. mouse.

    Organism-specific databases

    CTDi 5880.
    MGIi MGI:97846. Rac2.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00640000091266.
    HOGENOMi HOG000233974.
    HOVERGENi HBG009351.
    InParanoidi Q05144.
    KOi K07860.
    OMAi REDRSYL.
    OrthoDBi EOG764747.
    PhylomeDBi Q05144.
    TreeFami TF101109.

    Enzyme and pathway databases

    Reactomei REACT_204733. G alpha (12/13) signalling events.
    REACT_210090. Rho GTPase cycle.
    REACT_220092. GPVI-mediated activation cascade.

    Miscellaneous databases

    ChiTaRSi RAC2. mouse.
    NextBioi 296391.
    PROi Q05144.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q05144.
    CleanExi MM_RAC2.
    Genevestigatori Q05144.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003578. Small_GTPase_Rho.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00174. RHO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51420. RHO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A member of the ras gene superfamily is expressed specifically in T, B and myeloid hemopoietic cells."
      Shirsat N.V., Pignolo R.J., Kreider B.L., Rovera G.
      Oncogene 5:769-772(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Pancreas.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiRAC2_MOUSE
    AccessioniPrimary (citable) accession number: Q05144
    Secondary accession number(s): Q3TBC4, Q9D8X9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3