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Protein

Ras-related C3 botulinum toxin substrate 2

Gene

Rac2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase.

Enzyme regulationi

Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi115 – 1184GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: MGI
  2. GTP binding Source: MGI

GO - Biological processi

  1. actin cytoskeleton organization Source: MGI
  2. actin filament organization Source: MGI
  3. bone resorption Source: Ensembl
  4. cell projection assembly Source: MGI
  5. chemotaxis Source: MGI
  6. G-protein coupled receptor signaling pathway Source: MGI
  7. GTP catabolic process Source: GOC
  8. lymphocyte aggregation Source: MGI
  9. positive regulation of cell proliferation Source: MGI
  10. positive regulation of lamellipodium assembly Source: UniProtKB
  11. positive regulation of neutrophil chemotaxis Source: UniProtKB
  12. regulation of cell-substrate adhesion Source: MGI
  13. regulation of neutrophil migration Source: MGI
  14. regulation of respiratory burst Source: MGI
  15. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_204733. G alpha (12/13) signalling events.
REACT_210090. Rho GTPase cycle.
REACT_220092. GPVI-mediated activation cascade.
REACT_246650. Sema4D induced cell migration and growth-cone collapse.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related C3 botulinum toxin substrate 2
Alternative name(s):
Protein EN-7
p21-Rac2
Gene namesi
Name:Rac2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:97846. Rac2.

Subcellular locationi

Cytoplasm By similarity. Membrane By similarity; Lipid-anchor By similarity
Note: Membrane-associated when activated.By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: MGI
  3. extracellular vesicular exosome Source: MGI
  4. focal adhesion Source: MGI
  5. lamellipodium Source: MGI
  6. membrane Source: MGI
  7. nuclear envelope Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 189189Ras-related C3 botulinum toxin substrate 2PRO_0000042048Add
BLAST
Propeptidei190 – 1923Removed in mature formBy similarityPRO_0000042049

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei147 – 1471N6-acetyllysineBy similarity
Modified residuei189 – 1891Cysteine methyl esterBy similarity
Lipidationi189 – 1891S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiQ05144.
PaxDbiQ05144.
PRIDEiQ05144.

PTM databases

PhosphoSiteiQ05144.

Expressioni

Gene expression databases

BgeeiQ05144.
CleanExiMM_RAC2.
GenevestigatoriQ05144.

Interactioni

Subunit structurei

Interacts with DOCK2, which may activate it. Interacts with S100A8 and calprotectin (S100A8/9).By similarity

Protein-protein interaction databases

BioGridi202557. 8 interactions.
IntActiQ05144. 2 interactions.
MINTiMINT-202545.

Structurei

3D structure databases

ProteinModelPortaliQ05144.
SMRiQ05144. Positions 1-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector regionSequence Analysis

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiQ05144.
KOiK07860.
OMAiPVRRYFC.
OrthoDBiEOG764747.
PhylomeDBiQ05144.
TreeFamiTF101109.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05144-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDSKP
60 70 80 90 100
VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASY ENVRAKWFPE
110 120 130 140 150
VRHHCPSTPI ILVGTKLDLR DDKDTIEKLK EKKLAPITYP QGLALAKDID
160 170 180 190
SVKYLECSAL TQRGLKTVFD EAIRAVLCPQ PTRQQKRPCS LL
Length:192
Mass (Da):21,441
Last modified:February 1, 1994 - v1
Checksum:i2A1F1266AB9D7705
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601G → V in BAB25109. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53247 mRNA. Translation: CAA37337.1.
AK007561 mRNA. Translation: BAB25109.1.
AK144136 mRNA. Translation: BAE25721.1.
AK171321 mRNA. Translation: BAE42390.1.
BC005455 mRNA. Translation: AAH05455.1.
CCDSiCCDS27619.1.
PIRiA60194.
RefSeqiNP_033034.1. NM_009008.3.
UniGeneiMm.1972.

Genome annotation databases

EnsembliENSMUST00000043214; ENSMUSP00000036384; ENSMUSG00000033220.
GeneIDi19354.
KEGGimmu:19354.
UCSCiuc007wpp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53247 mRNA. Translation: CAA37337.1.
AK007561 mRNA. Translation: BAB25109.1.
AK144136 mRNA. Translation: BAE25721.1.
AK171321 mRNA. Translation: BAE42390.1.
BC005455 mRNA. Translation: AAH05455.1.
CCDSiCCDS27619.1.
PIRiA60194.
RefSeqiNP_033034.1. NM_009008.3.
UniGeneiMm.1972.

3D structure databases

ProteinModelPortaliQ05144.
SMRiQ05144. Positions 1-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202557. 8 interactions.
IntActiQ05144. 2 interactions.
MINTiMINT-202545.

PTM databases

PhosphoSiteiQ05144.

Proteomic databases

MaxQBiQ05144.
PaxDbiQ05144.
PRIDEiQ05144.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043214; ENSMUSP00000036384; ENSMUSG00000033220.
GeneIDi19354.
KEGGimmu:19354.
UCSCiuc007wpp.1. mouse.

Organism-specific databases

CTDi5880.
MGIiMGI:97846. Rac2.

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiQ05144.
KOiK07860.
OMAiPVRRYFC.
OrthoDBiEOG764747.
PhylomeDBiQ05144.
TreeFamiTF101109.

Enzyme and pathway databases

ReactomeiREACT_204733. G alpha (12/13) signalling events.
REACT_210090. Rho GTPase cycle.
REACT_220092. GPVI-mediated activation cascade.
REACT_246650. Sema4D induced cell migration and growth-cone collapse.

Miscellaneous databases

ChiTaRSiRac2. mouse.
NextBioi296391.
PROiQ05144.
SOURCEiSearch...

Gene expression databases

BgeeiQ05144.
CleanExiMM_RAC2.
GenevestigatoriQ05144.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A member of the ras gene superfamily is expressed specifically in T, B and myeloid hemopoietic cells."
    Shirsat N.V., Pignolo R.J., Kreider B.L., Rovera G.
    Oncogene 5:769-772(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Pancreas.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.

Entry informationi

Entry nameiRAC2_MOUSE
AccessioniPrimary (citable) accession number: Q05144
Secondary accession number(s): Q3TBC4, Q9D8X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 4, 2015
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.