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Protein

Clathrin coat assembly protein AP180

Gene

Snap91

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.

GO - Molecular functioni

GO - Biological processi

  • axonogenesis Source: BHF-UCL
  • chemical synaptic transmission Source: InterPro
  • clathrin coat assembly Source: InterPro
  • establishment or maintenance of cell polarity Source: BHF-UCL
  • protein transport Source: UniProtKB-KW
  • regulation of endocytosis Source: BHF-UCL
  • regulation of protein transport Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin coat assembly protein AP180
Alternative name(s):
91 kDa synaptosomal-associated protein
Clathrin coat-associated protein AP180
Gene namesi
Name:Snap91
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69276. Snap91.

Subcellular locationi

GO - Cellular componenti

  • clathrin-coated pit Source: UniProtKB-SubCell
  • clathrin-coated vesicle Source: InterPro
  • plasma membrane Source: BHF-UCL
  • postsynaptic density Source: BHF-UCL
  • postsynaptic membrane Source: BHF-UCL
  • presynaptic membrane Source: BHF-UCL
  • synaptic vesicle Source: BHF-UCL
  • terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 915915Clathrin coat assembly protein AP180PRO_0000193866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei296 – 2961PhosphoserineCombined sources
Modified residuei300 – 3001PhosphoserineCombined sources
Modified residuei306 – 3061Phosphoserine1 Publication
Glycosylationi310 – 3101O-linked (GlcNAc)1 Publication
Modified residuei313 – 3131PhosphoserineCombined sources1 Publication
Modified residuei317 – 3171PhosphothreonineBy similarity
Modified residuei594 – 5941PhosphoserineCombined sources
Modified residuei600 – 6001Phosphoserine1 Publication
Modified residuei640 – 6401Phosphoserine1 Publication
Modified residuei646 – 6461Phosphoserine1 Publication
Modified residuei775 – 7751PhosphoserineCombined sources
Isoform 2 (identifier: Q05140-2)
Modified residuei600 – 6001PhosphoserineCombined sources
Modified residuei627 – 6271PhosphoserineCombined sources

Post-translational modificationi

Thr-310 can be modified by the addition of N-acetylglucosamine which can be further phosphorylated. The form with phosphorylated O-linked N-acetylglucosamine is predominant in brain synaptosomes. There is no evidence for direct Thr-310 phosphorylation (PubMed:21500857).1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ05140.
PRIDEiQ05140.

PTM databases

iPTMnetiQ05140.
PhosphoSiteiQ05140.

Interactioni

Subunit structurei

Binds AP2A2. Interacts with AP2B1; clathrin competes with SNAP91.5 Publications

Protein-protein interaction databases

BioGridi249290. 7 interactions.
IntActiQ05140. 4 interactions.
MINTiMINT-101133.
STRINGi10116.ENSRNOP00000030821.

Structurei

3D structure databases

DisProtiDP00225.
ProteinModelPortaliQ05140.
SMRiQ05140. Positions 19-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 145132ENTHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi410 – 4134Poly-Thr
Compositional biasi535 – 5395Poly-Ala
Compositional biasi547 – 5504Poly-Ala
Compositional biasi678 – 6836Poly-Ser
Compositional biasi723 – 7297Poly-Ser

Domaini

Possesses a three domain structure: the N-terminal 300 residues harbor a clathrin binding site, an acidic middle domain 450 residues, interrupted by an Ala-rich segment, and the C-terminal domain (166 residues).

Sequence similaritiesi

Belongs to the PICALM/SNAP91 family.Curated
Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0251. Eukaryota.
ENOG410XQ90. LUCA.
HOGENOMiHOG000015763.
HOVERGENiHBG049391.
InParanoidiQ05140.
KOiK20043.
PhylomeDBiQ05140.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR030428. AP180_vert.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PANTHERiPTHR22951:SF4. PTHR22951:SF4. 2 hits.
PfamiPF07651. ANTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q05140-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGQTLTDRI AAAQYSVTGS AVARAVCKAT THEVMGPKKK HLDYLIQATN
60 70 80 90 100
ETNVNIPQMA DTLFERATNS SWVVVFKALV TTHHLMVHGN ERFIQYLASR
110 120 130 140 150
NTLFNLSNFL DKSGSHGYDM STFIRRYSRY LNEKAFSYRQ MAFDFARVKK
160 170 180 190 200
GADGVMRTMV PEKLLKSMPI LQGQIDALLE FDVHPNELTN GVINAAFMLL
210 220 230 240 250
FKDLIKLFAC YNDGVINLLE KFFEMKKGQC KDALEIYKRF LTRMTRVSEF
260 270 280 290 300
LKVADEVGID KGDIPDLTQA PSSLMETLEQ HLNTLEGKKP GNNEGSGAPS
310 320 330 340 350
PLSKSSPATT VTSPNSTPAK TIDTSPPVDI FATASAAAPV SSAKPSSDLL
360 370 380 390 400
DLQPDFSGAR AGAAAPVPPP TGGATAWGDL LGEDSLAALS SVPSEAPISD
410 420 430 440 450
PFAPEPSPPT TTTEPASASA SATTAVTAAT TEVDLFGDAF AASPGEAPAA
460 470 480 490 500
SEGATAPATP APVAAALDAC SGNDPFAPSE GSAEAAPELD LFAMKPPETS
510 520 530 540 550
APVVTPTAST APPVPATAPS PAPTAVAATA ATTTAAAAAT TTATTSAAAA
560 570 580 590 600
TTAAAPPALD IFGDLFDSAP EVAAASKPDV APSIDLFGTD AFSSPPRGAS
610 620 630 640 650
PVPESSLTAD LLSGSGFHCA EDDRHVPLFF TAVDAFAAPS PASTASPAKA
660 670 680 690 700
ESSGVIDLFG DAFGSSASET QPAPQAVSSS SASADLLAGF GGSFMAPSTT
710 720 730 740 750
PVTPAQNNLL QPNFEAAFGT TPSTSSSSSF DPSGDLLMPT MAPSGQPAPV
760 770 780 790 800
SMVPPSPAMS ASKGLGSDLD SSLASLVGNL GISGTTSKKG DLQWNAGEKK
810 820 830 840 850
LTGGANWQPK VTPATWSAGV PPQGTVPPTS SVPPGAGAPS VGQPGAGYGM
860 870 880 890 900
PPAGTGMTMM PQQPVMFAQP MMRPPFGAAA VPGTQLSPSP TPATQSPKKP
910
PAKDPLADLN IKDFL
Length:915
Mass (Da):93,519
Last modified:November 1, 1996 - v1
Checksum:i32EC1B38EC5DF8C0
GO
Isoform 2 (identifier: Q05140-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     614-632: Missing.

Show »
Length:896
Mass (Da):91,431
Checksum:i41417E79D1044984
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei614 – 63219Missing in isoform 2. 1 PublicationVSP_000173Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68877 mRNA. Translation: CAA48748.1.
X68878 mRNA. Translation: CAA48749.1.
PIRiS36326.
S36327.
RefSeqiNP_113916.1. NM_031728.1. [Q05140-1]
UniGeneiRn.11022.
Rn.164718.

Genome annotation databases

GeneIDi65178.
KEGGirno:65178.
UCSCiRGD:69276. rat. [Q05140-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68877 mRNA. Translation: CAA48748.1.
X68878 mRNA. Translation: CAA48749.1.
PIRiS36326.
S36327.
RefSeqiNP_113916.1. NM_031728.1. [Q05140-1]
UniGeneiRn.11022.
Rn.164718.

3D structure databases

DisProtiDP00225.
ProteinModelPortaliQ05140.
SMRiQ05140. Positions 19-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249290. 7 interactions.
IntActiQ05140. 4 interactions.
MINTiMINT-101133.
STRINGi10116.ENSRNOP00000030821.

PTM databases

iPTMnetiQ05140.
PhosphoSiteiQ05140.

Proteomic databases

PaxDbiQ05140.
PRIDEiQ05140.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi65178.
KEGGirno:65178.
UCSCiRGD:69276. rat. [Q05140-1]

Organism-specific databases

CTDi9892.
RGDi69276. Snap91.

Phylogenomic databases

eggNOGiKOG0251. Eukaryota.
ENOG410XQ90. LUCA.
HOGENOMiHOG000015763.
HOVERGENiHBG049391.
InParanoidiQ05140.
KOiK20043.
PhylomeDBiQ05140.

Miscellaneous databases

PROiQ05140.

Family and domain databases

Gene3Di1.20.58.150. 1 hit.
1.25.40.90. 1 hit.
InterProiIPR011417. ANTH_dom.
IPR030428. AP180_vert.
IPR014712. Clathrin_AP_2.
IPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PANTHERiPTHR22951:SF4. PTHR22951:SF4. 2 hits.
PfamiPF07651. ANTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAP180_RAT
AccessioniPrimary (citable) accession number: Q05140
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.