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Q05127

- VP35_EBOZM

UniProt

Q05127 - VP35_EBOZM

Protein

Polymerase cofactor VP35

Gene

VP35

Organism
Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Acsts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1, producing a strong inhibition of the phosphorylation and activation of IRF3. Also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR.6 Publications

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. evasion or tolerance by virus of host immune response Source: CACAO
    2. negative regulation of gene expression Source: CACAO
    3. negative regulation of gene silencing by miRNA Source: CACAO
    4. suppression by virus of host antigen processing and presentation of peptide antigen via MHC class II Source: CACAO
    5. suppression by virus of host cytokine production Source: CACAO
    6. suppression by virus of host IKBKE activity Source: UniProtKB-KW
    7. suppression by virus of host IRF3 activity by inhibition of IRF3 phosphorylation Source: CACAO
    8. suppression by virus of host IRF7 activity by positive regulation of IRF7 sumoylation Source: CACAO
    9. suppression by virus of host TBK1 activity Source: UniProtKB-KW
    10. suppression by virus of host toll-like receptor signaling pathway Source: UniProtKB-KW
    11. suppression by virus of host type I interferon production Source: CACAO
    12. suppression of host defenses Source: CACAO
    13. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host IKBKE by virus, Inhibition of host innate immune response by virus, Inhibition of host IRF7 by virus, Inhibition of host RLR pathway by virus, Inhibition of host TBK1 by virus, Inhibition of host TLR pathway by virus, Interferon antiviral system evasion, Transcription, Viral immunoevasion, Viral RNA replication

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polymerase cofactor VP35
    Gene namesi
    Name:VP35
    OrganismiZaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)
    Taxonomic identifieri128952 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesFiloviridaeEbolavirus
    Virus hostiEpomops franqueti (Franquet's epauleted fruit bat) [TaxID: 77231]
    Homo sapiens (Human) [TaxID: 9606]
    Myonycteris torquata (Little collared fruit bat) [TaxID: 77243]
    ProteomesiUP000007209: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. ribonucleoprotein complex Source: CACAO
    3. viral nucleocapsid Source: CACAO

    Keywords - Cellular componenti

    Host cytoplasm, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi90 – 934LASL → AASA: Complete loss of homotrimerization; when associated with A-107.
    Mutagenesisi107 – 1071L → A: Complete loss of homotrimerization; when associated with 90-AASA-93. 1 Publication
    Mutagenesisi305 – 3051R → A: No effect on IRF3 promoter inhibition. 1 Publication
    Mutagenesisi309 – 3091K → A: Partial loss of IRF3 promoter inhibition. Complete loss of dsRNA-binding. 2 Publications
    Mutagenesisi312 – 3121R → A: Complete loss of IRF3 promoter inhibition. Complete loss of dsRNA-binding. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 340340Polymerase cofactor VP35PRO_0000222161Add
    BLAST

    Post-translational modificationi

    Phosphorylated by host IKBKE.1 Publication

    Interactioni

    Subunit structurei

    Homooligomer. Homomultimerizes via the coiled coil domain. Found in a trimeric complex in which VP35 bridges L and the nucleoprotein By similarity. May interact with VP40. Interacts with host IKBKE and TBK1; the interactions lead to inhibition of cellular antiviral response by blocking necessary interactions of IKBKE and TBK1 with their substrate IRF3.By similarity3 Publications

    Protein-protein interaction databases

    DIPiDIP-48771N.
    IntActiQ05127. 54 interactions.

    Structurei

    Secondary structure

    1
    340
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi221 – 2299
    Beta strandi232 – 2365
    Helixi238 – 25215
    Helixi256 – 26914
    Helixi273 – 28311
    Helixi285 – 2873
    Beta strandi294 – 2996
    Helixi300 – 3023
    Helixi305 – 3106
    Helixi320 – 3223
    Beta strandi324 – 3307
    Beta strandi331 – 3333
    Beta strandi335 – 3395

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3FKEX-ray1.40A/B215-340[»]
    3L25X-ray2.00A/B/D/E215-340[»]
    3L26X-ray2.40A/B215-340[»]
    3L27X-ray1.95A/B/C/D215-340[»]
    3L28X-ray2.40A/B/C/D/E/F215-338[»]
    3L29X-ray1.70A/B215-340[»]
    4IBBX-ray1.75A/B215-340[»]
    4IBCX-ray1.74A/B215-340[»]
    4IBDX-ray1.84A/B215-340[»]
    4IBEX-ray1.95A/B215-340[»]
    4IBFX-ray2.29A/B215-340[»]
    4IBGX-ray1.41A/B215-340[»]
    4IBIX-ray1.47A/B215-340[»]
    4IBJX-ray1.54A/B215-340[»]
    4IBKX-ray1.85A/B215-340[»]
    4IJEX-ray1.90A/B/C/D218-340[»]
    4IJFX-ray2.51A218-340[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ05127.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili96 – 11621Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR002953. Filo_VP35.
    [Graphical view]
    PfamiPF02097. Filo_VP35. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018326. VP35_FiloV. 1 hit.
    PRINTSiPR01240. FILOVP35.

    Sequencei

    Sequence statusi: Complete.

    Q05127-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTRTKGRGH TAATTQNDRM PGPELSGWIS EQLMTGRIPV SDIFCDIENN    50
    PGLCYASQMQ QTKPNPKTRN SQTQTDPICN HSFEEVVQTL ASLATVVQQQ 100
    TIASESLEQR ITSLENGLKP VYDMAKTISS LNRVCAEMVA KYDLLVMTTG 150
    RATATAAATE AYWAEHGQPP PGPSLYEESA IRGKIESRDE TVPQSVREAF 200
    NNLNSTTSLT EENFGKPDIS AKDLRNIMYD HLPGFGTAFH QLVQVICKLG 250
    KDSNSLDIIH AEFQASLAEG DSPQCALIQI TKRVPIFQDA APPVIHIRSR 300
    GDIPRACQKS LRPVPPSPKI DRGWVCVFQL QDGKTLGLKI 340
    Length:340
    Mass (Da):37,362
    Last modified:February 1, 1994 - v1
    Checksum:iB171C7FDBB5B1FBD
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti12 – 121A → V in strain: Isolate mouse-adapted.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61274 Genomic RNA. Translation: CAA43578.1.
    L11365 Genomic RNA. Translation: AAB81002.1.
    AF086833 Genomic RNA. Translation: AAD14582.1.
    AF272001 Genomic RNA. Translation: AAG40165.1.
    AY142960 Genomic RNA. Translation: AAN37505.1.
    AF499101 Genomic RNA. Translation: AAM76032.1.
    RefSeqiNP_066244.1. NC_002549.1.

    Genome annotation databases

    GeneIDi911827.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61274 Genomic RNA. Translation: CAA43578.1 .
    L11365 Genomic RNA. Translation: AAB81002.1 .
    AF086833 Genomic RNA. Translation: AAD14582.1 .
    AF272001 Genomic RNA. Translation: AAG40165.1 .
    AY142960 Genomic RNA. Translation: AAN37505.1 .
    AF499101 Genomic RNA. Translation: AAM76032.1 .
    RefSeqi NP_066244.1. NC_002549.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3FKE X-ray 1.40 A/B 215-340 [» ]
    3L25 X-ray 2.00 A/B/D/E 215-340 [» ]
    3L26 X-ray 2.40 A/B 215-340 [» ]
    3L27 X-ray 1.95 A/B/C/D 215-340 [» ]
    3L28 X-ray 2.40 A/B/C/D/E/F 215-338 [» ]
    3L29 X-ray 1.70 A/B 215-340 [» ]
    4IBB X-ray 1.75 A/B 215-340 [» ]
    4IBC X-ray 1.74 A/B 215-340 [» ]
    4IBD X-ray 1.84 A/B 215-340 [» ]
    4IBE X-ray 1.95 A/B 215-340 [» ]
    4IBF X-ray 2.29 A/B 215-340 [» ]
    4IBG X-ray 1.41 A/B 215-340 [» ]
    4IBI X-ray 1.47 A/B 215-340 [» ]
    4IBJ X-ray 1.54 A/B 215-340 [» ]
    4IBK X-ray 1.85 A/B 215-340 [» ]
    4IJE X-ray 1.90 A/B/C/D 218-340 [» ]
    4IJF X-ray 2.51 A 218-340 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48771N.
    IntActi Q05127. 54 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 911827.

    Miscellaneous databases

    EvolutionaryTracei Q05127.

    Family and domain databases

    InterProi IPR002953. Filo_VP35.
    [Graphical view ]
    Pfami PF02097. Filo_VP35. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018326. VP35_FiloV. 1 hit.
    PRINTSi PR01240. FILOVP35.
    ProtoNeti Search...

    Publicationsi

    1. "The VP35 and VP40 proteins of filoviruses. Homology between Marburg and Ebola viruses."
      Bukreyev A.A., Volchkov V.E., Blinov V.M., Netesov S.V.
      FEBS Lett. 322:41-46(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus."
      Sanchez A., Kiley M.P., Holloway B.P., Auperin D.D.
      Virus Res. 29:215-240(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Characterization of the L gene and 5' trailer region of Ebola virus."
      Volchkov V.E., Volchkova V.A., Chepurnov A.A., Blinov V.M., Netesov S.V., Feldmann H.
      J. Gen. Virol. 80:355-362(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    4. "Molecular characterization of guinea pig-adapted variants of Ebola virus."
      Volchkov V.E., Chepurnov A.A., Volchkova V.A., Ternovoj V.A., Klenk H.D.
      Virology 277:147-155(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate guinea pig-adapted.
    5. Wilson J.A., Kondig J.P., Kuehne A.I., Hart M.K.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate mouse-adapted.
    6. "Comparison of the transcription and replication strategies of marburg virus and Ebola virus by using artificial replication systems."
      Muhlberger E., Weik M., Volchkov V.E., Klenk H.D., Becker S.
      J. Virol. 73:2333-2342(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN VIRAL REPLICATION.
    7. Cited for: FUNCTION.
    8. "The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein."
      Huang Y., Xu L., Sun Y., Nabel G.J.
      Mol. Cell 10:307-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THE NUCLEOPROTEIN.
    9. "The Ebola virus VP35 protein inhibits activation of interferon regulatory factor 3."
      Basler C.F., Mikulasova A., Martinez-Sobrido L., Paragas J., Muhlberger E., Bray M., Klenk H.D., Palese P., Garcia-Sastre A.
      J. Virol. 77:7945-7956(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "A C-terminal basic amino acid motif of Zaire ebolavirus VP35 is essential for type I interferon antagonism and displays high identity with the RNA-binding domain of another interferon antagonist, the NS1 protein of influenza A virus."
      Hartman A.L., Towner J.S., Nichol S.T.
      Virology 328:177-184(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ARG-305; LYS-309 AND ARG-312.
    11. "Homo-oligomerization facilitates the interferon-antagonist activity of the ebolavirus VP35 protein."
      Reid S.P., Cardenas W.B., Basler C.F.
      Virology 341:179-189(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: HOMOTRIMERIZATION, MUTAGENESIS OF 90-LEU--LEU-93 AND LEU-107.
    12. "Ebola virus VP35 Protein binds double-Stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signaling."
      Cardenas W.B., Loo Y.M., Gale M. Jr., Hartman A.L., Kimberlin C.R., Martinez-Sobrido L., Saphire E.O., Basler C.F.
      J. Virol. 80:5168-5178(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF RNA-BINDING ACTIVITY, MUTAGENESIS OF LYS-309 AND ARG-312.
    13. "Ebola virus VP35-VP40 interaction is sufficient for packaging 3E-5E minigenome RNA into virus-like particles."
      Johnson R.F., McCarthy S.E., Godlewski P.J., Harty R.N.
      J. Virol. 80:5135-5144(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH VP40.
    14. "VP35 knockdown inhibits Ebola virus amplification and protects against lethal infection in mice."
      Enterlein S., Warfield K.L., Swenson D.L., Stein D.A., Smith J.L., Gamble C.S., Kroeker A.D., Iversen P.L., Bavari S., Muhlberger E.
      Antimicrob. Agents Chemother. 50:984-993(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. "The VP35 protein of Ebola virus inhibits the antiviral effect mediated by double-stranded RNA-dependent protein kinase PKR."
      Feng Z., Cerveny M., Yan Z., He B.
      J. Virol. 81:182-192(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    16. "Ebola virus protein VP35 impairs the function of interferon regulatory factor-activating kinases IKKepsilon and TBK-1."
      Prins K.C., Cardenas W.B., Basler C.F.
      J. Virol. 83:3069-3077(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HOST IKBKE AND TBK1, PHOSPHORYLATION BY HOST IKBKE.

    Entry informationi

    Entry nameiVP35_EBOZM
    AccessioniPrimary (citable) accession number: Q05127
    Secondary accession number(s): Q77LU7, Q8JS63
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3