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Q05123

- ARP9_YEAST

UniProt

Q05123 - ARP9_YEAST

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Protein

Actin-like protein ARP9

Gene

ARP9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in transcriptional regulation. Heterodimer of ARP9 and ARP7 functions with HMG box proteins to facilitate proper chromatin architecture. Heterodimer formation is necessary for assembly into RSC complex. Part of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.8 Publications

GO - Molecular functioni

  1. structural molecule activity Source: UniProtKB

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. ATP-dependent chromatin remodeling Source: UniProtKB
  3. chromatin organization Source: UniProtKB
  4. nucleosome disassembly Source: SGD
  5. nucleosome mobilization Source: SGD
  6. nucleosome positioning Source: SGD
  7. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  8. regulation of transcription, DNA-templated Source: UniProtKB
  9. transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32738-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-like protein ARP9
Alternative name(s):
Chromatin structure-remodeling complex protein ARP9
SWI/SNF complex component ARP9
Gene namesi
Name:ARP9
Synonyms:SWP59
Ordered Locus Names:YMR033W
ORF Names:YM9973.07
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR033w.
SGDiS000004636. ARP9.

Subcellular locationi

Nucleus 1 Publication
Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.

GO - Cellular componenti

  1. nucleus Source: SGD
  2. RSC complex Source: UniProtKB
  3. SWI/SNF complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi337 – 3371G → V: Impaired heterodimerization with ARP7; when associated with L-338. 1 Publication
Mutagenesisi338 – 3381G → L: Impaired heterodimerization with ARP7; when associated with V-337. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 467467Actin-like protein ARP9PRO_0000089129Add
BLAST

Proteomic databases

MaxQBiQ05123.
PaxDbiQ05123.
PeptideAtlasiQ05123.

Expressioni

Gene expression databases

GenevestigatoriQ05123.

Interactioni

Subunit structurei

Forms a heterodimer with ARP7. Interacts with LDB7 and NPL6. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARP7Q124065EBI-2972,EBI-2962

Protein-protein interaction databases

BioGridi35205. 109 interactions.
DIPiDIP-6709N.
IntActiQ05123. 60 interactions.
MINTiMINT-669840.
STRINGi4932.YMR033W.

Structurei

Secondary structure

1
467
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 83Combined sources
Beta strandi10 – 145Combined sources
Beta strandi16 – 249Combined sources
Beta strandi27 – 293Combined sources
Beta strandi35 – 395Combined sources
Beta strandi41 – 455Combined sources
Beta strandi51 – 566Combined sources
Helixi58 – 603Combined sources
Beta strandi62 – 654Combined sources
Beta strandi72 – 743Combined sources
Helixi76 – 9823Combined sources
Turni101 – 1066Combined sources
Beta strandi111 – 1155Combined sources
Helixi121 – 13313Combined sources
Beta strandi139 – 1446Combined sources
Helixi145 – 1528Combined sources
Beta strandi157 – 1648Combined sources
Beta strandi169 – 1757Combined sources
Turni181 – 1833Combined sources
Beta strandi185 – 1884Combined sources
Helixi191 – 20111Combined sources
Helixi207 – 2148Combined sources
Helixi224 – 2285Combined sources
Beta strandi229 – 2324Combined sources
Beta strandi251 – 2544Combined sources
Helixi276 – 2783Combined sources
Beta strandi282 – 2854Combined sources
Beta strandi291 – 2944Combined sources
Helixi296 – 2994Combined sources
Helixi303 – 31715Combined sources
Helixi323 – 3297Combined sources
Beta strandi333 – 3375Combined sources
Helixi338 – 3414Combined sources
Helixi345 – 35713Combined sources
Helixi363 – 37210Combined sources
Helixi379 – 3868Combined sources
Beta strandi401 – 4033Combined sources
Helixi417 – 4226Combined sources
Turni425 – 4273Combined sources
Helixi428 – 44013Combined sources
Beta strandi448 – 4503Combined sources
Helixi451 – 4577Combined sources
Helixi458 – 4647Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WEEX-ray3.10A1-467[»]
4I6MX-ray2.80B1-467[»]
ProteinModelPortaliQ05123.
SMRiQ05123. Positions 6-467.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiCOG5277.
HOGENOMiHOG000034076.
InParanoidiQ05123.
KOiK11768.
OMAiDHEMATE.
OrthoDBiEOG7JHMF8.

Family and domain databases

InterProiIPR004000. Actin-related.
IPR027665. Arp9_fungi.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF182. PTHR11937:SF182. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05123-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPFRQDSIL IIYPRSQTTL VQFGLNEETF TVPELEIPTQ IYRTTRQDGS
60 70 80 90 100
YTYHSTNKDN KAELIKPIQN GEIIDISAFT QFLRLIFVSI LSDRANKNQD
110 120 130 140 150
AFEAELSNIP LLLITHHSWS QSDLEIITQY VFESLEINNL IQLPASLAAT
160 170 180 190 200
YSMISLQNCC IIDVGTHHTD IIPIVDYAQL DHLVSSIPMG GQSINDSLKK
210 220 230 240 250
LLPQWDDDQI ESLKKSPIFE VLSDDAKKLS SFDFGNENED EDEGTLNVAE
260 270 280 290 300
IITSGRDTRE VLEERERGQK VKNVKNSDLE FNTFWDEKGN EIKVGKQRFQ
310 320 330 340 350
GCNNLIKNIS NRVGLTLDNI DDINKAKAVW ENIIIVGGTT SISGFKEALL
360 370 380 390 400
GQLLKDHLII EPEEEKSKRE EEAKSVLPAA TKKKSKFMTN STAFVPTIEY
410 420 430 440 450
VQCPTVIKLA KYPDYFPEWK KSGYSEIIFL GAQIVSKQIF THPKDTFYIT
460
REKYNMKGPA ALWDVQF
Length:467
Mass (Da):53,074
Last modified:November 1, 1997 - v1
Checksum:iEE1AB154A906420E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49213 Genomic DNA. Translation: CAA89149.1.
BK006946 Genomic DNA. Translation: DAA09932.1.
PIRiS53950.
RefSeqiNP_013747.1. NM_001182530.1.

Genome annotation databases

EnsemblFungiiYMR033W; YMR033W; YMR033W.
GeneIDi855049.
KEGGisce:YMR033W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49213 Genomic DNA. Translation: CAA89149.1 .
BK006946 Genomic DNA. Translation: DAA09932.1 .
PIRi S53950.
RefSeqi NP_013747.1. NM_001182530.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3WEE X-ray 3.10 A 1-467 [» ]
4I6M X-ray 2.80 B 1-467 [» ]
ProteinModelPortali Q05123.
SMRi Q05123. Positions 6-467.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35205. 109 interactions.
DIPi DIP-6709N.
IntActi Q05123. 60 interactions.
MINTi MINT-669840.
STRINGi 4932.YMR033W.

Proteomic databases

MaxQBi Q05123.
PaxDbi Q05123.
PeptideAtlasi Q05123.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR033W ; YMR033W ; YMR033W .
GeneIDi 855049.
KEGGi sce:YMR033W.

Organism-specific databases

CYGDi YMR033w.
SGDi S000004636. ARP9.

Phylogenomic databases

eggNOGi COG5277.
HOGENOMi HOG000034076.
InParanoidi Q05123.
KOi K11768.
OMAi DHEMATE.
OrthoDBi EOG7JHMF8.

Enzyme and pathway databases

BioCyci YEAST:G3O-32738-MONOMER.

Miscellaneous databases

NextBioi 978283.

Gene expression databases

Genevestigatori Q05123.

Family and domain databases

InterProi IPR004000. Actin-related.
IPR027665. Arp9_fungi.
[Graphical view ]
PANTHERi PTHR11937. PTHR11937. 1 hit.
PTHR11937:SF182. PTHR11937:SF182. 1 hit.
Pfami PF00022. Actin. 1 hit.
[Graphical view ]
SMARTi SM00268. ACTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF."
    Cairns B.R., Erdjument-Bromage H., Tempst P., Winston F., Kornberg R.D.
    Mol. Cell 2:639-651(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-37 AND 459-467, FUNCTION, IDENTIFICATION IN THE RSC AND SWI/SNF COMPLEXES.
  4. Cited for: FUNCTION OF THE RSC COMPLEX, COMPOSITION OF THE RSC COMPLEX.
  5. "Who's who among the Saccharomyces cerevisiae actin-related proteins? A classification and nomenclature proposal for a large family."
    Poch O., Winsor B.
    Yeast 13:1053-1058(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  6. "Histone octamer transfer by a chromatin-remodeling complex."
    Lorch Y., Zhang M., Kornberg R.D.
    Cell 96:389-392(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  7. "Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC."
    Moreira J.M.A., Holmberg S.
    EMBO J. 18:2836-2844(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  8. "Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains."
    Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F.
    Mol. Cell 4:715-723(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF THE RSC COMPLEX.
  9. "Chromatin remodeling by RSC involves ATP-dependent DNA translocation."
    Saha A., Wittmeyer J., Cairns B.R.
    Genes Dev. 16:2120-2134(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  10. "Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway."
    Chai B., Hsu J.-M., Du J., Laurent B.C.
    Genetics 161:575-584(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  11. "The nuclear actin-related proteins Arp7 and Arp9: a dimeric module that cooperates with architectural proteins for chromatin remodeling."
    Szerlong H., Saha A., Cairns B.R.
    EMBO J. 22:3175-3187(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, HETERODIMERIC COMPLEX FORMATION WITH ARP7 WITHIN THE RSC COMPLEX, MUTAGENESIS OF GLY-337 AND GLY-338.
  12. "The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation."
    Hsu J.-M., Huang J., Meluh P.B., Laurent B.C.
    Mol. Cell. Biol. 23:3202-3215(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles."
    Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.
    Genetics 172:795-809(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH LDB7 AND NPL6.

Entry informationi

Entry nameiARP9_YEAST
AccessioniPrimary (citable) accession number: Q05123
Secondary accession number(s): D6VZK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1790 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3