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Q05123 (ARP9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Actin-like protein ARP9
Alternative name(s):
Chromatin structure-remodeling complex protein ARP9
SWI/SNF complex component ARP9
Gene names
Name:ARP9
Synonyms:SWP59
Ordered Locus Names:YMR033W
ORF Names:YM9973.07
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length467 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in transcriptional regulation. Heterodimer of ARP9 and ARP7 functions with HMG box proteins to facilitate proper chromatin architecture. Heterodimer formation is necessary for assembly into RSC complex. Part of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. Ref.3 Ref.4 Ref.6 Ref.7 Ref.9 Ref.10 Ref.11 Ref.12

Subunit structure

Forms a heterodimer with ARP7. Interacts with LDB7 and NPL6. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29. Ref.3 Ref.12 Ref.14

Subcellular location

Nucleus. Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1. Ref.12

Miscellaneous

Present with 1790 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the actin family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ARP7Q124065EBI-2972,EBI-2962

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 467467Actin-like protein ARP9
PRO_0000089129

Experimental info

Mutagenesis3371G → V: Impaired heterodimerization with ARP7; when associated with L-338. Ref.11
Mutagenesis3381G → L: Impaired heterodimerization with ARP7; when associated with V-337. Ref.11

Secondary structure

.......................................................................... 467
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q05123 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: EE1AB154A906420E

FASTA46753,074
        10         20         30         40         50         60 
MAPFRQDSIL IIYPRSQTTL VQFGLNEETF TVPELEIPTQ IYRTTRQDGS YTYHSTNKDN 

        70         80         90        100        110        120 
KAELIKPIQN GEIIDISAFT QFLRLIFVSI LSDRANKNQD AFEAELSNIP LLLITHHSWS 

       130        140        150        160        170        180 
QSDLEIITQY VFESLEINNL IQLPASLAAT YSMISLQNCC IIDVGTHHTD IIPIVDYAQL 

       190        200        210        220        230        240 
DHLVSSIPMG GQSINDSLKK LLPQWDDDQI ESLKKSPIFE VLSDDAKKLS SFDFGNENED 

       250        260        270        280        290        300 
EDEGTLNVAE IITSGRDTRE VLEERERGQK VKNVKNSDLE FNTFWDEKGN EIKVGKQRFQ 

       310        320        330        340        350        360 
GCNNLIKNIS NRVGLTLDNI DDINKAKAVW ENIIIVGGTT SISGFKEALL GQLLKDHLII 

       370        380        390        400        410        420 
EPEEEKSKRE EEAKSVLPAA TKKKSKFMTN STAFVPTIEY VQCPTVIKLA KYPDYFPEWK 

       430        440        450        460 
KSGYSEIIFL GAQIVSKQIF THPKDTFYIT REKYNMKGPA ALWDVQF 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF."
Cairns B.R., Erdjument-Bromage H., Tempst P., Winston F., Kornberg R.D.
Mol. Cell 2:639-651(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 16-37 AND 459-467, FUNCTION, IDENTIFICATION IN THE RSC AND SWI/SNF COMPLEXES.
[4]"RSC, an essential, abundant chromatin-remodeling complex."
Cairns B.R., Lorch Y., Li Y., Zhang M., Lacomis L., Erdjument-Bromage H., Tempst P., Du J., Laurent B.C., Kornberg R.D.
Cell 87:1249-1260(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RSC COMPLEX, COMPOSITION OF THE RSC COMPLEX.
[5]"Who's who among the Saccharomyces cerevisiae actin-related proteins? A classification and nomenclature proposal for a large family."
Poch O., Winsor B.
Yeast 13:1053-1058(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE NAME.
[6]"Histone octamer transfer by a chromatin-remodeling complex."
Lorch Y., Zhang M., Kornberg R.D.
Cell 96:389-392(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RSC COMPLEX.
[7]"Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC."
Moreira J.M.A., Holmberg S.
EMBO J. 18:2836-2844(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RSC COMPLEX.
[8]"Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains."
Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F.
Mol. Cell 4:715-723(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: COMPOSITION OF THE RSC COMPLEX.
[9]"Chromatin remodeling by RSC involves ATP-dependent DNA translocation."
Saha A., Wittmeyer J., Cairns B.R.
Genes Dev. 16:2120-2134(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RSC COMPLEX.
[10]"Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway."
Chai B., Hsu J.-M., Du J., Laurent B.C.
Genetics 161:575-584(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RSC COMPLEX.
[11]"The nuclear actin-related proteins Arp7 and Arp9: a dimeric module that cooperates with architectural proteins for chromatin remodeling."
Szerlong H., Saha A., Cairns B.R.
EMBO J. 22:3175-3187(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, HETERODIMERIC COMPLEX FORMATION WITH ARP7 WITHIN THE RSC COMPLEX, MUTAGENESIS OF GLY-337 AND GLY-338.
[12]"The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation."
Hsu J.-M., Huang J., Meluh P.B., Laurent B.C.
Mol. Cell. Biol. 23:3202-3215(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
[13]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[14]"The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles."
Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.
Genetics 172:795-809(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH LDB7 AND NPL6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49213 Genomic DNA. Translation: CAA89149.1.
BK006946 Genomic DNA. Translation: DAA09932.1.
PIRS53950.
RefSeqNP_013747.1. NM_001182530.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3WEEX-ray3.10A1-467[»]
4I6MX-ray2.80B1-467[»]
ProteinModelPortalQ05123.
SMRQ05123. Positions 3-467.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35205. 108 interactions.
DIPDIP-6709N.
IntActQ05123. 60 interactions.
MINTMINT-669840.
STRING4932.YMR033W.

Proteomic databases

PaxDbQ05123.
PeptideAtlasQ05123.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR033W; YMR033W; YMR033W.
GeneID855049.
KEGGsce:YMR033W.

Organism-specific databases

CYGDYMR033w.
SGDS000004636. ARP9.

Phylogenomic databases

eggNOGCOG5277.
HOGENOMHOG000034076.
KOK11768.
OMAYFPEWKK.
OrthoDBEOG7JHMF8.

Enzyme and pathway databases

BioCycYEAST:G3O-32738-MONOMER.

Gene expression databases

GenevestigatorQ05123.

Family and domain databases

InterProIPR004000. Actin-related.
[Graphical view]
PANTHERPTHR11937. PTHR11937. 1 hit.
PfamPF00022. Actin. 1 hit.
[Graphical view]
SMARTSM00268. ACTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978283.

Entry information

Entry nameARP9_YEAST
AccessionPrimary (citable) accession number: Q05123
Secondary accession number(s): D6VZK8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references