Q05117 (PPA5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tartrate-resistant acid phosphatase type 5
Short name=TR-AP
EC=3.1.3.2

Alternative name(s):
Tartrate-resistant acid ATPase
Short name=TrATPase
Type 5 acid phosphatase

Gene names

Name:	Acp5
Synonyms:	T5ap, Trap

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play a role in the process of bone resorption. The osteoclastic trap acts on nucleotide tri- and diphosphates with higher affinity, compared with other substrates.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 2 iron ions per subunit.
Subunit structure	Exists either as monomer or, after proteolytic processing, as a dimer of two chains linked by disulfide bond(s).
Subcellular location	Lysosome.
Tissue specificity	Characteristic constituent of osteoclasts.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Ligand	Iron Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bone morphogenesis Inferred from mutant phenotype PubMed 8898228. Source: MGI bone resorption Inferred from mutant phenotype PubMed 8898228. Source: MGI defense response to Gram-positive bacterium Inferred from mutant phenotype PubMed 11168643. Source: MGI negative regulation of inflammatory response Inferred from mutant phenotype PubMed 11168643. Source: MGI negative regulation of interleukin-1 beta production Inferred from mutant phenotype PubMed 11168643. Source: MGI negative regulation of interleukin-12 production Inferred from mutant phenotype PubMed 11168643. Source: MGI negative regulation of nitric oxide biosynthetic process Inferred from mutant phenotype PubMed 11168643. Source: MGI negative regulation of superoxide anion generation Inferred from mutant phenotype PubMed 11168643. Source: MGI negative regulation of tumor necrosis factor production Inferred from mutant phenotype PubMed 11168643. Source: MGI response to cytokine stimulus Inferred from direct assay PubMed 8200916. Source: MGI response to lipopolysaccharide Inferred from mutant phenotype PubMed 11168643. Source: MGI
Cellular_component	lysosome Inferred from direct assay PubMed 11168643. Source: MGI
Molecular_function	acid phosphatase activity Inferred from direct assay PubMed 11168643PubMed 9308894. Source: MGI ferric iron binding Inferred from sequence or structural similarity. Source: UniProtKB ferrous iron binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 327	305	Tartrate-resistant acid phosphatase type 5		PRO_0000023982
Sites
	Metal binding	35	1	Iron 1 By similarity
	Metal binding	73	1	Iron 1 By similarity
	Metal binding	73	1	Iron 2 By similarity
	Metal binding	76	1	Iron 1 By similarity
	Metal binding	112	1	Iron 2 By similarity
	Metal binding	207	1	Iron 2 By similarity
	Metal binding	242	1	Iron 2 By similarity
	Metal binding	244	1	Iron 1 By similarity
Amino acid modifications
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	149	1	N-linked (GlcNAc...) Potential
	Disulfide bond	163 ↔ 221		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05117 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 6D3975C4EF714C56	FASTA	327	36,807
	10 20 30 40 50 60 MDSWVVLLGL QIIWLPLLTH GTAPTPTLRF VAVGDWGGVP NAPFHTAREM ANAKEIARTV 70 80 90 100 110 120 QTMGADFIMS LGDNFYFTGV HDASDKRFQE TFEDVFSDRA LRNIPWYVLA GNHDHLGNVS 130 140 150 160 170 180 AQIAYSKISK RWNFPSPYYR LRFKIPRTNI TVAIFMLDTV MLCGNSDDFA SQQPKMPRDL 190 200 210 220 230 240 GVARTQLSWL KKQLAAAKED YVLVAGHYPI WSIAEHGPTR CLVKNLRPLL ATYGVTAYLC 250 260 270 280 290 300 GHDHNLQYLQ DENGVGYVLS GAGNFMDPSV RHQRKVPNGY LRFHYGSEDS LGGFTHVEIS 310 320 PKEMTIIYVE ASGKSLFKTS LPRRPRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the genes encoding mouse and human type-5 acid phosphatase." Cassady A.I., King A.G., Cross N.C.P., Hume D.A. Gene 130:201-207(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[3]	"Cloning and characterization of the 5'-flanking region of the mouse tartrate-resistant acid phosphatase gene." Reddy S.V., Scarcez T., Windle J.J., Leach R.J., Hundley J.E., Chirgwin J.M., Chou J.Y., Roodman G.D. J. Bone Miner. Res. 8:1263-1270(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48. Tissue: Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M99054 Genomic DNA. Translation: AAA37245.1. BC012911 mRNA. Translation: AAH12911.1. BC019160 mRNA. Translation: AAH19160.1. BC029644 mRNA. Translation: AAH29644.1. M85212 Genomic DNA. Translation: AAA40479.1.
IPI	IPI00137491.
PIR	JN0787.
RefSeq	NP_001095874.1. NM_001102404.1. NP_001095875.1. NM_001102405.1. NP_031414.1. NM_007388.3.
UniGene	Mm.46354.
3D structure databases
ProteinModelPortal	Q05117.
SMR	Q05117. Positions 27-325.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000065425.
PTM databases
PhosphoSite	Q05117.
Proteomic databases
PaxDb	Q05117.
PRIDE	Q05117.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000069330; ENSMUSP00000065425; ENSMUSG00000001348. ENSMUST00000115315; ENSMUSP00000110970; ENSMUSG00000001348. ENSMUST00000165735; ENSMUSP00000127128; ENSMUSG00000001348.
GeneID	11433.
KEGG	mmu:11433.
UCSC	uc009onz.1. mouse.
Organism-specific databases
CTD	54.
MGI	MGI:87883. Acp5.
Phylogenomic databases
eggNOG	COG1409.
GeneTree	ENSGT00390000016735.
HOGENOM	HOG000007592.
HOVERGEN	HBG000433.
InParanoid	Q05117.
KO	K14379.
OMA	DVNDKRF.
OrthoDB	EOG4Z36FB.
Gene expression databases
ArrayExpress	Q05117.
Bgee	Q05117.
CleanEx	MM_ACP5.
Genevestigator	Q05117.
GermOnline	ENSMUSG00000001348. Mus musculus.
Family and domain databases
InterPro	IPR024927. Acid_Pase_5. IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF000898. Acid_Ptase_5. 1 hit.
ProtoNet	Search...
Other
NextBio	278720.
SOURCE	Search...

Entry information

Entry name

PPA5_MOUSE

Accession

Primary (citable) accession number: Q05117

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program