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Q05117 (PPA5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tartrate-resistant acid phosphatase type 5

Short name=TR-AP
EC=3.1.3.2
Alternative name(s):
Tartrate-resistant acid ATPase
Short name=TrATPase
Type 5 acid phosphatase
Gene names
Name:Acp5
Synonyms:T5ap, Trap
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May play a role in the process of bone resorption. The osteoclastic trap acts on nucleotide tri- and diphosphates with higher affinity, compared with other substrates.

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 2 iron ions per subunit.

Subunit structure

Exists either as monomer or, after proteolytic processing, as a dimer of two chains linked by disulfide bond(s).

Subcellular location

Lysosome.

Tissue specificity

Characteristic constituent of osteoclasts.

Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   LigandIron
Metal-binding
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbone morphogenesis

Inferred from mutant phenotype PubMed 8898228. Source: MGI

bone resorption

Inferred from mutant phenotype PubMed 8898228. Source: MGI

defense response to Gram-positive bacterium

Inferred from mutant phenotype PubMed 11168643. Source: MGI

dephosphorylation

Inferred from direct assay PubMed 9308894. Source: MGI

negative regulation of inflammatory response

Inferred from mutant phenotype PubMed 11168643. Source: MGI

negative regulation of interleukin-1 beta production

Inferred from mutant phenotype PubMed 11168643. Source: MGI

negative regulation of interleukin-12 production

Inferred from mutant phenotype PubMed 11168643. Source: MGI

negative regulation of nitric oxide biosynthetic process

Inferred from mutant phenotype PubMed 11168643. Source: MGI

negative regulation of superoxide anion generation

Inferred from mutant phenotype PubMed 11168643. Source: MGI

negative regulation of tumor necrosis factor production

Inferred from mutant phenotype PubMed 11168643. Source: MGI

response to cytokine

Inferred from direct assay PubMed 8200916. Source: MGI

response to lipopolysaccharide

Inferred from mutant phenotype PubMed 11168643. Source: MGI

   Cellular_componentlysosome

Inferred from direct assay PubMed 11168643. Source: MGI

   Molecular_functionacid phosphatase activity

Inferred from direct assay PubMed 11168643PubMed 9308894. Source: MGI

ferric iron binding

Inferred from sequence or structural similarity. Source: UniProtKB

ferrous iron binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 By similarity
Chain23 – 327305Tartrate-resistant acid phosphatase type 5
PRO_0000023982

Sites

Metal binding351Iron 1 By similarity
Metal binding731Iron 1 By similarity
Metal binding731Iron 2 By similarity
Metal binding761Iron 1 By similarity
Metal binding1121Iron 2 By similarity
Metal binding2071Iron 2 By similarity
Metal binding2421Iron 2 By similarity
Metal binding2441Iron 1 By similarity

Amino acid modifications

Glycosylation1181N-linked (GlcNAc...) Potential
Glycosylation1491N-linked (GlcNAc...) Potential
Disulfide bond163 ↔ 221 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05117 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 6D3975C4EF714C56

FASTA32736,807
        10         20         30         40         50         60 
MDSWVVLLGL QIIWLPLLTH GTAPTPTLRF VAVGDWGGVP NAPFHTAREM ANAKEIARTV 

        70         80         90        100        110        120 
QTMGADFIMS LGDNFYFTGV HDASDKRFQE TFEDVFSDRA LRNIPWYVLA GNHDHLGNVS 

       130        140        150        160        170        180 
AQIAYSKISK RWNFPSPYYR LRFKIPRTNI TVAIFMLDTV MLCGNSDDFA SQQPKMPRDL 

       190        200        210        220        230        240 
GVARTQLSWL KKQLAAAKED YVLVAGHYPI WSIAEHGPTR CLVKNLRPLL ATYGVTAYLC 

       250        260        270        280        290        300 
GHDHNLQYLQ DENGVGYVLS GAGNFMDPSV RHQRKVPNGY LRFHYGSEDS LGGFTHVEIS 

       310        320 
PKEMTIIYVE ASGKSLFKTS LPRRPRP 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the genes encoding mouse and human type-5 acid phosphatase."
Cassady A.I., King A.G., Cross N.C.P., Hume D.A.
Gene 130:201-207(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary gland.
[3]"Cloning and characterization of the 5'-flanking region of the mouse tartrate-resistant acid phosphatase gene."
Reddy S.V., Scarcez T., Windle J.J., Leach R.J., Hundley J.E., Chirgwin J.M., Chou J.Y., Roodman G.D.
J. Bone Miner. Res. 8:1263-1270(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48.
Tissue: Spleen.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M99054 Genomic DNA. Translation: AAA37245.1.
BC012911 mRNA. Translation: AAH12911.1.
BC019160 mRNA. Translation: AAH19160.1.
BC029644 mRNA. Translation: AAH29644.1.
M85212 Genomic DNA. Translation: AAA40479.1.
CCDSCCDS22923.1.
PIRJN0787.
RefSeqNP_001095874.1. NM_001102404.1.
NP_001095875.1. NM_001102405.1.
NP_031414.1. NM_007388.3.
XP_006510007.1. XM_006509944.1.
XP_006510008.1. XM_006509945.1.
XP_006510009.1. XM_006509946.1.
UniGeneMm.46354.

3D structure databases

ProteinModelPortalQ05117.
SMRQ05117. Positions 27-325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ05117. 2 interactions.
MINTMINT-1851665.
STRING10090.ENSMUSP00000065425.

PTM databases

PhosphoSiteQ05117.

Proteomic databases

MaxQBQ05117.
PaxDbQ05117.
PRIDEQ05117.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000069330; ENSMUSP00000065425; ENSMUSG00000001348.
ENSMUST00000115315; ENSMUSP00000110970; ENSMUSG00000001348.
ENSMUST00000165735; ENSMUSP00000127128; ENSMUSG00000001348.
GeneID11433.
KEGGmmu:11433.
UCSCuc009onz.1. mouse.

Organism-specific databases

CTD54.
MGIMGI:87883. Acp5.

Phylogenomic databases

eggNOGCOG1409.
GeneTreeENSGT00390000016735.
HOGENOMHOG000007592.
HOVERGENHBG000433.
InParanoidQ05117.
KOK14379.
OMAFYFSGVQ.
OrthoDBEOG7WHH9R.
PhylomeDBQ05117.
TreeFamTF313175.

Gene expression databases

ArrayExpressQ05117.
BgeeQ05117.
CleanExMM_ACP5.
GenevestigatorQ05117.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR024927. Acid_Pase_5.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PIRSFPIRSF000898. Acid_Ptase_5. 1 hit.
SUPFAMSSF56300. SSF56300. 1 hit.
ProtoNetSearch...

Other

NextBio278720.
PROQ05117.
SOURCESearch...

Entry information

Entry namePPA5_MOUSE
AccessionPrimary (citable) accession number: Q05117
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot