Dual specificity mitogen-activated protein kinase kinase 1 - Xenopus laevis (African clawed frog)

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Q05116 (MP2K1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Dual specificity mitogen-activated protein kinase kinase 1
Short name=MAP kinase kinase 1
Short name=MAPKK 1
EC=2.7.12.2

Alternative name(s):
ERK activator kinase 1
MAPK/ERK kinase 1
Short name=MEK1

Gene names

Name:

map2k1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates the MAPK/ERK cascade, ultimately leading to phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton › spindle pole body By similarity. Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Expressed in the central nervous system, kidney, liver, intestine and the hematopoietic system.
Post-translational modification	Activated by phosphorylation on Ser/Thr catalyzed by MAP kinase kinase kinases (RAF or MOS).
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell spindle pole body Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 395

394

Dual specificity mitogen-activated protein kinase kinase 1

PRO_0000086371

Regions

Domain

68 – 363

296

Protein kinase

Nucleotide binding

74 – 82

ATP By similarity

Compositional bias

262 – 309

Pro-rich

Sites

Active site

190

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

218

Phosphoserine; by RAF By similarity

Modified residue

222

Phosphoserine; by RAF By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q05116 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: F09607CCD1C206BE
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FASTA

395

43,742

        10         20         30         40         50         60 
MPKKKPTPIQ LNPNPEGTAV NGTPTAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV 

        70         80         90        100        110        120 
GELKDDDFEK VSELGAGNGG VVFKVSHKPT SLIMARKLIH LEIKPAIRNQ IIRELQVLHE 

       130        140        150        160        170        180 
CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGK IPEKILGKVS IAVIKGLTYL 

       190        200        210        220        230        240 
REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY 

       250        260        270        280        290        300 
SVQSDIWSMG LSLVEMAIGR YPIPPPDAKE LELIFGCSVE RDPASSELAP RPRPPGRPIS 

       310        320        330        340        350        360 
SYGPDSRPPM AIFELLDYIV NEPPPKLPSG VFGAEFQDFV NKCLVKNPAE RADLKQLMVH 

       370        380        390 
SFIKQSELEE VDFAGWLCST MGLKQPSTPT HAAGV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates." Kosako H., Nishida E., Gotoh Y. EMBO J. 12:787-794(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13700 mRNA. Translation: BAA02860.1. BC043913 mRNA. Translation: AAH43913.1.
PIR	S36186.
RefSeq	NP_001080299.1. NM_001086830.1.
UniGene	Xl.5877.
3D structure databases
ProteinModelPortal	Q05116.
SMR	Q05116. Positions 62-383.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-121021.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	379991.
KEGG	xla:379991.
Organism-specific databases
CTD	5604.
Xenbase	XB-GENE-865216. map2k1.
Phylogenomic databases
HOVERGEN	HBG108518.
KO	K04368.
Enzyme and pathway databases
BRENDA	2.7.12.2. 6726.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
PMAP-CutDB	Q05116.

Entry information

Entry name

MP2K1_XENLA

Accession

Primary (citable) accession number: Q05116
Secondary accession number(s): Q5D0B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents