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Q05116 (MP2K1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual specificity mitogen-activated protein kinase kinase 1

Short name=MAP kinase kinase 1
Short name=MAPKK 1
EC=2.7.12.2
Alternative name(s):
ERK activator kinase 1
MAPK/ERK kinase 1
Short name=MEK1
Gene names
Name:map2k1
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates the MAPK/ERK cascade, ultimately leading to phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body By similarity. Cytoplasm By similarity. Nucleus By similarity.

Tissue specificity

Expressed in the central nervous system, kidney, liver, intestine and the hematopoietic system.

Post-translational modification

Activated by phosphorylation on Ser/Thr catalyzed by MAP kinase kinase kinases (RAF or MOS).

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 395394Dual specificity mitogen-activated protein kinase kinase 1
PRO_0000086371

Regions

Domain68 – 363296Protein kinase
Nucleotide binding74 – 829ATP By similarity
Compositional bias262 – 30948Pro-rich

Sites

Active site1901Proton acceptor By similarity
Binding site971ATP By similarity

Amino acid modifications

Modified residue2181Phosphoserine; by RAF By similarity
Modified residue2221Phosphoserine; by RAF By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05116 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: F09607CCD1C206BE

FASTA39543,742
        10         20         30         40         50         60 
MPKKKPTPIQ LNPNPEGTAV NGTPTAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV 

        70         80         90        100        110        120 
GELKDDDFEK VSELGAGNGG VVFKVSHKPT SLIMARKLIH LEIKPAIRNQ IIRELQVLHE 

       130        140        150        160        170        180 
CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGK IPEKILGKVS IAVIKGLTYL 

       190        200        210        220        230        240 
REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY 

       250        260        270        280        290        300 
SVQSDIWSMG LSLVEMAIGR YPIPPPDAKE LELIFGCSVE RDPASSELAP RPRPPGRPIS 

       310        320        330        340        350        360 
SYGPDSRPPM AIFELLDYIV NEPPPKLPSG VFGAEFQDFV NKCLVKNPAE RADLKQLMVH 

       370        380        390 
SFIKQSELEE VDFAGWLCST MGLKQPSTPT HAAGV 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates."
Kosako H., Nishida E., Gotoh Y.
EMBO J. 12:787-794(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13700 mRNA. Translation: BAA02860.1.
BC043913 mRNA. Translation: AAH43913.1.
PIRS36186.
RefSeqNP_001080299.1. NM_001086830.1.
UniGeneXl.5877.

3D structure databases

ProteinModelPortalQ05116.
SMRQ05116. Positions 62-383.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid98233. 5 interactions.
MINTMINT-121021.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID379991.
KEGGxla:379991.

Organism-specific databases

CTD5604.
XenbaseXB-GENE-865216. map2k1.

Phylogenomic databases

HOVERGENHBG108518.
KOK04368.

Enzyme and pathway databases

BRENDA2.7.12.2. 6726.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PMAP-CutDBQ05116.

Entry information

Entry nameMP2K1_XENLA
AccessionPrimary (citable) accession number: Q05116
Secondary accession number(s): Q5D0B8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families