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Protein

Dual specificity mitogen-activated protein kinase kinase 1

Gene

map2k1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates the MAPK/ERK cascade, ultimately leading to phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei97ATPPROSITE-ProRule annotation1
Active sitei190Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 82ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.12.2. 6725.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity mitogen-activated protein kinase kinase 1 (EC:2.7.12.2)
Short name:
MAP kinase kinase 1
Short name:
MAPKK 1
Alternative name(s):
ERK activator kinase 1
MAPK/ERK kinase 1
Short name:
MEK1
Gene namesi
Name:map2k1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-865216. map2k1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000863712 – 395Dual specificity mitogen-activated protein kinase kinase 1Add BLAST394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218Phosphoserine; by RAFBy similarity1
Modified residuei222Phosphoserine; by RAFBy similarity1

Post-translational modificationi

Activated by phosphorylation on Ser/Thr catalyzed by MAP kinase kinase kinases (RAF or MOS).

Keywords - PTMi

Phosphoprotein

Miscellaneous databases

PMAP-CutDBiQ05116.

Expressioni

Tissue specificityi

Expressed in the central nervous system, kidney, liver, intestine and the hematopoietic system.

Interactioni

Protein-protein interaction databases

BioGridi98233. 5 interactors.
ELMiQ05116.
MINTiMINT-121021.

Structurei

3D structure databases

ProteinModelPortaliQ05116.
SMRiQ05116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 363Protein kinasePROSITE-ProRule annotationAdd BLAST296

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi262 – 309Pro-richAdd BLAST48

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG108518.
KOiK04368.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05116-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKKKPTPIQ LNPNPEGTAV NGTPTAETNL EALQKKLEEL ELDEQQRKRL
60 70 80 90 100
EAFLTQKQKV GELKDDDFEK VSELGAGNGG VVFKVSHKPT SLIMARKLIH
110 120 130 140 150
LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS
160 170 180 190 200
LDQVLKKAGK IPEKILGKVS IAVIKGLTYL REKHKIMHRD VKPSNILVNS
210 220 230 240 250
RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG
260 270 280 290 300
LSLVEMAIGR YPIPPPDAKE LELIFGCSVE RDPASSELAP RPRPPGRPIS
310 320 330 340 350
SYGPDSRPPM AIFELLDYIV NEPPPKLPSG VFGAEFQDFV NKCLVKNPAE
360 370 380 390
RADLKQLMVH SFIKQSELEE VDFAGWLCST MGLKQPSTPT HAAGV
Length:395
Mass (Da):43,742
Last modified:January 23, 2007 - v2
Checksum:iF09607CCD1C206BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13700 mRNA. Translation: BAA02860.1.
BC043913 mRNA. Translation: AAH43913.1.
PIRiS36186.
RefSeqiNP_001080299.1. NM_001086830.1.
UniGeneiXl.5877.

Genome annotation databases

GeneIDi379991.
KEGGixla:379991.

Similar proteinsi

Entry informationi

Entry nameiMP2K1_XENLA
AccessioniPrimary (citable) accession number: Q05116
Secondary accession number(s): Q5D0B8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families