Reviewed, UniProtKB/Swiss-Prot Q05116 (MP2K1_XENLA)

Names and origin

Protein names

Recommended name:
    Dual specificity mitogen-activated protein kinase kinase 1
      Short name=MAP kinase kinase 1
      Short name=MAPKK 1
    EC=2.7.12.2
Alternative name(s):
    ERK activator kinase 1
    MAPK/ERK kinase 1
      Short name=MEK1

Gene names

Name:

map2k1

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Expressed in the central nervous system, kidney, liver, intestine and the hematopoietic system.
Post-translational modification	Activated by phosphorylation on Ser/Thr catalyzed by MAP kinase kinase kinases (RAF or MOS).
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 395	394	Dual specificity mitogen-activated protein kinase kinase 1		PRO_0000086371
Regions
	Domain	68 – 363	296	Protein kinase
	Nucleotide binding	74 – 82	9	ATP By similarity
	Compositional bias	262 – 309	48	Pro-rich
Sites
	Active site	190	1	Proton acceptor By similarity
	Binding site	97	1	ATP By similarity
Amino acid modifications
	Modified residue	218	1	Phosphoserine; by RAF By similarity
	Modified residue	222	1	Phosphoserine; by RAF By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05116-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: F09607CCD1C206BE	FASTA	395	43,742
	10 20 30 40 50 60 MPKKKPTPIQ LNPNPEGTAV NGTPTAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV 70 80 90 100 110 120 GELKDDDFEK VSELGAGNGG VVFKVSHKPT SLIMARKLIH LEIKPAIRNQ IIRELQVLHE 130 140 150 160 170 180 CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGK IPEKILGKVS IAVIKGLTYL 190 200 210 220 230 240 REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY 250 260 270 280 290 300 SVQSDIWSMG LSLVEMAIGR YPIPPPDAKE LELIFGCSVE RDPASSELAP RPRPPGRPIS 310 320 330 340 350 360 SYGPDSRPPM AIFELLDYIV NEPPPKLPSG VFGAEFQDFV NKCLVKNPAE RADLKQLMVH 370 380 390 SFIKQSELEE VDFAGWLCST MGLKQPSTPT HAAGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates." Kosako H., Nishida E., Gotoh Y. EMBO J. 12:787-794(1993) [PubMed: 8440264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D13700 mRNA. Translation: BAA02860.1. BC043913 mRNA. Translation: AAH43913.1.
PIR	S36186.
RefSeq	NP_001080299.1.
UniGene	Xl.5877
3D structure databases
SMR	Q05116. Positions 62-383.
ModBase	Search...
Genome annotation databases
GeneID	379991.
KEGG	xla:379991.
Organism-specific databases
CTD	379991.
Xenbase	XB-FEAT-478821. map2k1.
Phylogenomic databases
HOVERGEN	Q05116.
Enzyme and pathway databases
BRENDA	2.7.12.2. 648.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
PMAP-CutDB	Q05116.

Entry information

Entry name

MP2K1_XENLA

Accession

Primary (citable) accession number: Q05116
Secondary accession number(s): Q5D0B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 23, 2007
Last modified:	September 1, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project