Q05110 (VA5_VESVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Venom allergen 5 Alternative name(s): Allergen Ves v V Antigen 5 Short name=Ag5 Cysteine-rich venom protein Short name=CRVP Allergen=Ves v 5
Organism	Vespula vulgaris (Yellow jacket) (Wasp)
Taxonomic identifier	7454 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Vespula

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the CRISP family. Contains 1 SCP domain.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Chain

24 – 227

204

Venom allergen 5

PRO_0000006297

Regions

Domain

68 – 212

145

SCP

Amino acid modifications

Disulfide bond

27 ↔ 40

Ref.3

Disulfide bond

31 ↔ 124

Ref.3

Disulfide bond

49 ↔ 117

Ref.3

Disulfide bond

193 ↔ 210

Ref.3

Experimental info

Sequence conflict

109

1

V → I in CAB42887. Ref.2

Sequence conflict

118

1

Q → E in CAB42887. Ref.2

Sequence conflict

173

1

D → N in CAB42887. Ref.2

Sequence conflict

219

1

M → K in CAB42887. Ref.2

Secondary structure

1

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227

Helix Strand Turn

Details...

Helix

26 – 28

3

Helix

38 – 41

4

Beta strand

53 – 58

6

Helix

61 – 79

19

Beta strand

94 – 96

3

Helix

104 – 114

11

Beta strand

128 – 130

3

Beta strand

133 – 144

12

Helix

149 – 157

9

Helix

158 – 162

5

Helix

169 – 171

3

Helix

174 – 183

10

Beta strand

190 – 200

11

Beta strand

203 – 214

12

Sequences

	Sequence		Length	Mass (Da)
	Q05110 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 99E9813740A66F55	FASTA	227	25,798
	10 20 30 40 50 60 MEISGLVYLI IIVTIIDLPY GKANNYCKIK CLKGGVHTAC KYGSLKPNCG NKVVVSYGLT 70 80 90 100 110 120 KQEKQDILKE HNDFRQKIAR GLETRGNPGP QPPAKNMKNL VWNDELAYVA QVWANQCQYG 130 140 150 160 170 180 HDTCRDVAKY QVGQNVALTG STAAKYDDPV KLVKMWEDEV KDYNPKKKFS GNDFLKTGHY 190 200 210 220 TQMVWANTKE VGCGSIKYIQ EKWHKHYLVC NYGPSGNFMN EELYQTK

References

[1]	"Sequence analysis and antigenic cross-reactivity of a venom allergen, antigen 5, from hornets, wasps, and yellow jackets." Lu G., Villalba M., Coscia M.R., Hoffman D.R., King T.P. J. Immunol. 150:2823-2830(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Venom and Venom gland.
[2]	"Molecular cloning of a genomic sequence from the venom allergen antigen 5 from Vespula vulgaris." Suck R., Hagen S., Fiebig H. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-227. Tissue: Venom gland.
[3]	"Major venom allergen of yellow jackets, Ves v 5: structural characterization of a pathogenesis-related protein superfamily." Henriksen A., King T.P., Mirza O., Monsalve R.I., Meno K., Ipsen H., Larsen J.N., Gajhede M., Spangfort M.D. Proteins 45:438-448(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 24-227, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M98858 mRNA. Translation: AAA30333.1.
AJ238849 Genomic DNA. Translation: CAB42887.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QNX	X-ray	1.90	A	24-227	[»]

ProteinModelPortal

Q05110.

SMR

Q05110. Positions 24-227.

ModBase

Search...

Protein family/group databases

Allergome

3523. Ves v 5.0101.
670. Ves v 5.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.40.33.10. 1 hit.

InterPro

IPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR002413. V5_allergen.
[Graphical view]

PANTHER

PTHR10334. PTHR10334. 1 hit.

Pfam

PF00188. CAP. 1 hit.
[Graphical view]

PRINTS

PR00838. V5ALLERGEN.
PR00837. V5TPXLIKE.

SMART

SM00198. SCP. 1 hit.
[Graphical view]

SUPFAM

SSF55797. SCP-like_extracellular. 1 hit.

PROSITE

PS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q05110.

Entry information

Entry name

VA5_VESVU

Accession

Primary (citable) accession number: Q05110
Secondary accession number(s): Q9UB91

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)