Q05097 (PA1L_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PA-I galactophilic lectin
Short name=PA-IL

Alternative name(s):
Galactose-binding lectin

Gene names

Name:	lecA
Synonyms:	pa1L
Ordered Locus Names:	PA2570

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity.
Subcellular location	Cytoplasm. Periplasm. Cell surface. Note: Low exposure on the cell surface.

Ontologies

Keywords
Cellular component	Cytoplasm Periplasm
Ligand	Lectin
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1

Chain

2 – 122

121

PA-I galactophilic lectin

PRO_0000058158

Secondary structure

1

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122

Helix Strand Turn

Details...

Beta strand

3 – 8

6

Beta strand

15 – 21

7

Beta strand

27 – 41

15

Beta strand

65 – 70

6

Beta strand

81 – 85

5

Beta strand

93 – 99

7

Helix

106 – 108

3

Beta strand

110 – 119

10

Sequences

	Sequence		Length	Mass (Da)
	Q05097 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 1DC739390388A451	FASTA	122	12,893
	10 20 30 40 50 60 MAWKGEVLAN NEAGQVTSII YNPGDVITIV AAGWASYGPT QKWGPQGDRE HPDQGLICHD 70 80 90 100 110 120 AFCGALVMKI GNSGTIPVNT GLFRWVAPNN VQGAITLIYN DVPGTYGNNS GSFSVNIGKD QS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of the amino acid sequence of the Pseudomonas aeruginosa galactophilic PA-I lectin." Avichezer D., Katcoff D.J., Garber N.C., Gilboa-Garber N. J. Biol. Chem. 267:23023-23027(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-31. Strain: ATCC 33347.
[2]	"Pseudomonas aeruginosa PA-I lectin gene molecular analysis and expression in Escherichia coli." Avichezer D., Gilboa-Garber N., Garber N.C., Katcoff D.J. Biochim. Biophys. Acta 1218:11-20(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"The Pseudomonas aeruginosa lectins PA-1L and PA-2L are controlled by quorum sensing and by RpoS." Winzer K., Falconer C., Diggle S.P., Garber N.C., Camara M., Williams P. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X65933 Genomic DNA. Translation: CAA46728.1.
AF229814 Genomic DNA. Translation: AAF70459.1.
AE004091 Genomic DNA. Translation: AAG05958.1.

PIR

S45300.

RefSeq

NP_251260.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1L7L	X-ray	1.50	A	2-121	[»]
1OKO	X-ray	1.60	A/B/C/D	2-122	[»]
1UOJ	X-ray	2.40	A/B/C/D	2-122	[»]
2VXJ	X-ray	1.90	A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X	2-122	[»]
2WYF	X-ray	2.40	A/B/C/D/E/F/G/H	2-122	[»]
3ZYB	X-ray	2.29	A/B/C/D/E/F/G/H	1-122	[»]
3ZYF	X-ray	1.94	A/B/C/D	1-122	[»]
3ZYH	X-ray	1.50	A/B	1-122	[»]
4A6S	X-ray	2.15	A/B/C/D	2-122	[»]
4AL9	X-ray	1.75	A/B/C/D/E/F/G/H	2-122	[»]

ProteinModelPortal

Q05097.

SMR

Q05097. Positions 2-122.

ModBase

Search...

Protein-protein interaction databases

STRING

208964.PA2570.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

882335.

KEGG

pae:PA2570.

Organism-specific databases

PseudoCAP

PA2570.

Phylogenomic databases

eggNOG

NOG123349.

HOGENOM

HOG000247286.

OMA

IVAKGWI.

ProtClustDB

CLSK546878.

Family and domain databases

Gene3D

2.60.120.430. 1 hit.

InterPro

IPR008979. Galactose-bd-like.
IPR012905. PA-IL.
[Graphical view]

Pfam

PF07828. PA-IL. 1 hit.
[Graphical view]

PIRSF

PIRSF020485. PA-IL. 1 hit.

SUPFAM

SSF49785. Gal_bind_like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q05097.

Entry information

Entry name

PA1L_PSEAE

Accession

Primary (citable) accession number: Q05097

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program