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Protein

Unconventional myosin-Ib

Gene

Myo1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Motor protein that may participate in process critical to neuronal development and function such as cell migration, neurite outgrowth and vesicular transport.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1158ATPSequence analysis

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • actin-dependent ATPase activity Source: RGD
  • ATP binding Source: UniProtKB
  • calmodulin binding Source: UniProtKB
  • microfilament motor activity Source: UniProtKB
  • motor activity Source: UniProtKB
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: UniProtKB
  • phosphatidylinositol-4,5-bisphosphate binding Source: UniProtKB

GO - Biological processi

  • actin filament-based movement Source: UniProtKB
  • actin filament bundle assembly Source: UniProtKB
  • nervous system development Source: RGD
  • post-Golgi vesicle-mediated transport Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-Ib
Alternative name(s):
Myosin I alpha
Short name:
MMI-alpha
Short name:
MMIa
Myosin heavy chain myr 1
Gene namesi
Name:Myo1b
Synonyms:Myo1a, Myr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi70994. Myo1b.

Subcellular locationi

GO - Cellular componenti

  • actin filament Source: Ensembl
  • brush border Source: RGD
  • cell periphery Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytoskeleton Source: RGD
  • early endosome Source: Ensembl
  • endosome membrane Source: Ensembl
  • extracellular exosome Source: Ensembl
  • filopodium Source: UniProtKB
  • myosin complex Source: UniProtKB-KW
  • perinuclear region of cytoplasm Source: Ensembl
  • plasma membrane Source: UniProtKB
  • trans-Golgi network membrane Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11361136Unconventional myosin-IbPRO_0000123444Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei60 – 601PhosphoserineBy similarity
Cross-linki287 – 287Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki287 – 287Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ05096.
PeptideAtlasiQ05096.
PRIDEiQ05096.

PTM databases

iPTMnetiQ05096.
PhosphoSiteiQ05096.

Expressioni

Gene expression databases

ExpressionAtlasiQ05096. baseline and differential.
GenevisibleiQ05096. RN.

Interactioni

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • calmodulin binding Source: UniProtKB

Protein-protein interaction databases

MINTiMINT-4563663.
STRINGi10116.ENSRNOP00000061523.

Structurei

Secondary structure

1
1136
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni10 – 134Combined sources
Helixi20 – 223Combined sources
Helixi28 – 4013Combined sources
Beta strandi45 – 484Combined sources
Beta strandi51 – 555Combined sources
Helixi66 – 727Combined sources
Turni77 – 793Combined sources
Beta strandi80 – 823Combined sources
Helixi84 – 9815Combined sources
Beta strandi102 – 1098Combined sources
Helixi112 – 12817Combined sources
Helixi133 – 14412Combined sources
Helixi146 – 1538Combined sources
Beta strandi166 – 1749Combined sources
Beta strandi180 – 1889Combined sources
Helixi193 – 1964Combined sources
Helixi206 – 2149Combined sources
Helixi217 – 2226Combined sources
Helixi229 – 2313Combined sources
Helixi247 – 26014Combined sources
Helixi265 – 28117Combined sources
Beta strandi299 – 3013Combined sources
Helixi304 – 31310Combined sources
Helixi317 – 3259Combined sources
Beta strandi326 – 3283Combined sources
Beta strandi331 – 3344Combined sources
Beta strandi337 – 3393Combined sources
Helixi342 – 37029Combined sources
Beta strandi381 – 3877Combined sources
Beta strandi395 – 3973Combined sources
Helixi399 – 42931Combined sources
Helixi442 – 4509Combined sources
Turni452 – 4543Combined sources
Helixi456 – 46510Combined sources
Beta strandi466 – 4683Combined sources
Helixi472 – 48211Combined sources
Turni492 – 4943Combined sources
Beta strandi507 – 5148Combined sources
Beta strandi517 – 5226Combined sources
Helixi526 – 5305Combined sources
Helixi536 – 5438Combined sources
Helixi548 – 5536Combined sources
Helixi555 – 5573Combined sources
Helixi570 – 58617Combined sources
Beta strandi588 – 5969Combined sources
Helixi609 – 61911Combined sources
Helixi621 – 6299Combined sources
Beta strandi633 – 6375Combined sources
Helixi638 – 6458Combined sources
Turni650 – 6556Combined sources
Helixi660 – 67011Combined sources
Beta strandi677 – 6804Combined sources
Beta strandi682 – 6887Combined sources
Helixi690 – 72839Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4L79X-ray2.30A1-728[»]
ProteinModelPortaliQ05096.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 701687Myosin motorAdd
BLAST
Domaini704 – 72926IQ 1PROSITE-ProRule annotationAdd
BLAST
Domaini730 – 75021IQ 2PROSITE-ProRule annotationAdd
BLAST
Domaini750 – 77829IQ 3PROSITE-ProRule annotationAdd
BLAST
Domaini780 – 80728IQ 4PROSITE-ProRule annotationAdd
BLAST
Domaini808 – 83730IQ 5PROSITE-ProRule annotationAdd
BLAST
Domaini837 – 86630IQ 6PROSITE-ProRule annotationAdd
BLAST
Domaini952 – 1136185TH1PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni592 – 5998Actin-bindingSequence analysis

Sequence similaritiesi

Contains 6 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated
Contains 1 TH1 (class I myosin tail homology) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0164. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129697.
HOGENOMiHOG000260264.
HOVERGENiHBG062373.
InParanoidiQ05096.
KOiK10356.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00612. IQ. 2 hits.
PF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 6 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS50096. IQ. 5 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: Q05096-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKKEVKSSL LDNMIGVGDT VLLEPLNEET FIDNLKKRFD HNEIYTYIGS
60 70 80 90 100
VVISVNPYRS LPIYSPEKVE DYRNRNFYEL SPHIFALSDE AYRSLRDQDK
110 120 130 140 150
DQCILITGES GAGKTEASKL VMSYVAAVCG KGAEVNQVKE QLLQSTPVLE
160 170 180 190 200
AFGNAKTVRN DNSSRFGKYM DIEFDFKGDP LGGVISNYLL EKSRVVKQPR
210 220 230 240 250
GERNFHVFYQ LLSGASEELL HKLKLERDFS RYNYLSLDSA KVNGVDDAAN
260 270 280 290 300
FRTVRNAMQI VGFSDPEAES VLEVVAAVLK LGNIEFKPES RMNGLDESKI
310 320 330 340 350
KDKNELKEIC ELTSIDQVVL ERAFSFRTVE AKQEKVSTTL NVAQAYYARD
360 370 380 390 400
ALAKNLYSRL FSWLVNRINE SIKAQTKVRK KVMGVLDIYG FEIFEDNSFE
410 420 430 440 450
QFIINYCNEK LQQIFIELTL KEEQEEYIRE DIEWTHIDYF NNAIICDLIE
460 470 480 490 500
NNTNGILAML DEECLRPGTV TDETFLEKLN QVCATHQHFE SRMSKCSRFL
510 520 530 540 550
NDTTLPHSCF RIQHYAGKVL YQVEGFVDKN NDLLYRDLSQ AMWKAGHALI
560 570 580 590 600
KSLFPEGNPA KVNLKRPPTA GSQFKASVAT LMKNLQTKNP NYIRCIKPND
610 620 630 640 650
KKAAHIFSES LVCHQIRYLG LLENVRVRRA GYAFRQAYEP CLERYKMLCK
660 670 680 690 700
QTWPHWKGPA RSGVEVLFNE LEIPVEEYSF GRSKIFIRNP RTLFQLEDLR
710 720 730 740 750
KQRLEDLATL IQKIYRGWKC RTHFLLMKRS QVVIAAWYRR YAQQKRYQQI
760 770 780 790 800
KSSALVIQSY IRGWKARKIL RELKHQKRCK EAATTIAAYW HGTQARKERR
810 820 830 840 850
RLKDEARNKH AIAVIWAFWL GSKARRELKR LKEEARRKHA VAVIWAYWLG
860 870 880 890 900
LKVRREYRKF FRANAGKKIY EFTLQRIVQK YLLEMKNKMP SLSPIDKNWP
910 920 930 940 950
SRPYLFLDST HKELKRIFHL WRCKKYRDQF TDQQKLIYEE KLEASELFKD
960 970 980 990 1000
KKALYPSSVG QPFQGAYLEI NKNPKYKKLK DAIEEKIIIA EVVNKINRAN
1010 1020 1030 1040 1050
GKSTSRIFLL TNNNLLLADQ KSGQIKSEVP LVDVTKVSMS SQNDGFFAVH
1060 1070 1080 1090 1100
LKEGSEAASK GDFLFSSDHL IEMATKLYRT TLSQTKQKLN IEISDEFLVQ
1110 1120 1130
FRQDKVCVKF IQGNQKNGSV PTCKRKNNRL LEVAVP
Length:1,136
Mass (Da):131,918
Last modified:November 1, 1996 - v1
Checksum:i69D6C2A84E9070E2
GO
Isoform B (identifier: Q05096-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     794-823: Missing.

Show »
Length:1,106
Mass (Da):128,301
Checksum:i16243ECD22FD4B67
GO
Isoform C (identifier: Q05096-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     794-852: Missing.

Show »
Length:1,077
Mass (Da):124,770
Checksum:i63D984F5A7B9443F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei794 – 85259Missing in isoform C. 1 PublicationVSP_003349Add
BLAST
Alternative sequencei794 – 82330Missing in isoform B. 1 PublicationVSP_003348Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68199 mRNA. Translation: CAA48287.1.
PIRiA45439.
C45439.
RefSeqiNP_446438.1. NM_053986.1. [Q05096-1]
UniGeneiRn.36339.

Genome annotation databases

EnsembliENSRNOT00000068433; ENSRNOP00000061523; ENSRNOG00000048152. [Q05096-1]
GeneIDi117057.
KEGGirno:117057.
UCSCiRGD:70994. rat. [Q05096-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68199 mRNA. Translation: CAA48287.1.
PIRiA45439.
C45439.
RefSeqiNP_446438.1. NM_053986.1. [Q05096-1]
UniGeneiRn.36339.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4L79X-ray2.30A1-728[»]
ProteinModelPortaliQ05096.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4563663.
STRINGi10116.ENSRNOP00000061523.

PTM databases

iPTMnetiQ05096.
PhosphoSiteiQ05096.

Proteomic databases

PaxDbiQ05096.
PeptideAtlasiQ05096.
PRIDEiQ05096.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000068433; ENSRNOP00000061523; ENSRNOG00000048152. [Q05096-1]
GeneIDi117057.
KEGGirno:117057.
UCSCiRGD:70994. rat. [Q05096-1]

Organism-specific databases

CTDi4430.
RGDi70994. Myo1b.

Phylogenomic databases

eggNOGiKOG0164. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00840000129697.
HOGENOMiHOG000260264.
HOVERGENiHBG062373.
InParanoidiQ05096.
KOiK10356.

Miscellaneous databases

PROiQ05096.

Gene expression databases

ExpressionAtlasiQ05096. baseline and differential.
GenevisibleiQ05096. RN.

Family and domain databases

InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR001609. Myosin_head_motor_dom.
IPR010926. Myosin_TH1.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00612. IQ. 2 hits.
PF00063. Myosin_head. 1 hit.
PF06017. Myosin_TH1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 6 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS50096. IQ. 5 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS51757. TH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification, characterization and cloning of myr 1, a mammalian myosin-I."
    Ruppert C., Kroschewski R., Baehler M.
    J. Cell Biol. 120:1393-1403(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C).
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiMYO1B_RAT
AccessioniPrimary (citable) accession number: Q05096
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-1 (MYH1).Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.