Reviewed, UniProtKB/Swiss-Prot Q05086 (UBE3A_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin-protein ligase E3A
    EC=6.3.2.-
Alternative name(s):
    E6AP ubiquitin-protein ligase
    Oncogenic protein-associated protein E6-AP
    Human papillomavirus E6-associated protein
    Renal carcinoma antigen NY-REN-54

Gene names

Name:	UBE3A
Synonyms:	E6AP, EPVE6AP, HPVE6A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	875 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Interacts with the E6 protein of the cancer-associated human papillomavirus types 16 and 18. The E6/E6-AP complex binds to and targets the p53 tumor-suppressor protein for ubiquitin-mediated proteolysis. It is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It can target itself for ubiquitination in vitro and efficiently promotes its own degradation in vivo. It appears that only unmodified E6-AP molecules can bind efficiently to p53 in the presence of the HPV E6 oncoprotein.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Binds UBQLN1 and UBQLN2. Interacts with the 26S proteasome. Interacts with HCV core protein and targets it to degradation.
Subcellular location	NucleusProbable.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR.
Involvement in disease	Defects in UBE3A are a cause of Angelman syndrome (AS) [MIM:105830]; also known as 'happy puppet syndrome'. AS is characterized by features of severe motor and intellectual retardation, microcephaly, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, hyperactivity, hypopigmentation, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, and an unusual facies characterized by macrostomia, a large mandible and open-mouthed expression, a great propensity for protruding the tongue ('tongue thrusting'), and an occipital groove.
Miscellaneous	A cysteine residue is required for ubiquitin-thioester formation.
Sequence similarities	Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Ubl conjugation pathway
Cellular component	Nucleus Proteasome
Coding sequence diversity	Alternative splicing Polymorphism
Disease	Disease mutation
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	brain development Ref.2 Traceable author statement. Source: ProtInc interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW protein modification process Inferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Traceable author statement. Source: ProtInc
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-KW protein complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
E6	P06463	1	EBI-954357,EBI-1186926	From a different organism.
TP53	P04637	1	EBI-954357,EBI-366083

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform II (identifier: Q05086-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform I (identifier: Q05086-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Isoform III (identifier: Q05086-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MEKLHQCYWK → MATACKR

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 875

875

Ubiquitin-protein ligase E3A

PRO_0000194980

Regions

Domain

776 – 875

100

HECT

Region

401 – 418

18

E6-binding

Region

418 – 517

100

Interaction with HCV core protein

Compositional bias

394 – 399

6

Asp/Glu-rich (acidic)

Sites

Active site

843

1

Glycyl thioester intermediate

Amino acid modifications

Modified residue

218

1

Phosphoserine

Natural variations

Alternative sequence

1 – 23

23

Missing in isoform I.

VSP_006705

Alternative sequence

1 – 10

10

MEKLHQCYWK → MATACKR in isoform III.

VSP_006706

Natural variant

44

1

C → Y Probable polymorphism.

VAR_007852

Natural variant

62

1

R → H

VAR_008142

Natural variant

201

1

A → T

VAR_007853

Natural variant

290

1

V → G: dbSNP rs1059383.

VAR_047516

Natural variant

372

1

S → P

VAR_008143

Natural variant

826

1

I → II in AS.

VAR_008144

Experimental info

Sequence conflict

359

1

R → RNLVNEFNSR AA sequence Ref.5

Sequence conflict

423

1

P → L in AAA35542. Ref.5

Sequence conflict

647 – 649

3

TFR → LFV in AAA35542. Ref.5

Sequence conflict

669

1

E → V in AAA35542. Ref.5

Sequence conflict

686

1

D → N in AAA35542. Ref.5

Secondary structure

1

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875

Helix Strand Turn

Details...

Beta strand

525 – 527

3

Beta strand

529 – 531

3

Helix

532 – 545

14

Helix

548 – 552

5

Beta strand

557 – 559

3

Helix

569 – 582

14

Helix

585 – 587

3

Beta strand

589 – 592

4

Turn

594 – 596

3

Beta strand

599 – 601

3

Helix

609 – 624

16

Helix

635 – 641

7

Helix

648 – 654

7

Helix

656 – 667

12

Helix

672 – 675

4

Beta strand

679 – 684

6

Turn

687 – 689

3

Beta strand

692 – 697

6

Turn

698 – 702

5

Turn

707 – 709

3

Helix

710 – 722

13

Helix

727 – 741

15

Beta strand

742 – 744

3

Helix

753 – 760

8

Turn

768 – 770

3

Beta strand

775 – 779

5

Helix

785 – 795

11

Helix

799 – 809

11

Beta strand

810 – 815

6

Helix

820 – 823

4

Beta strand

826 – 833

8

Beta strand

839 – 841

3

Helix

842 – 844

3

Beta strand

846 – 851

6

Helix

855 – 868

14

Sequences

	Sequence		Length	Mass (Da)
	Isoform II [UniParc]. Last modified November 25, 2008. Version 4. Checksum: F80F0502B3B3838A	FASTA	875	100,688
	10 20 30 40 50 60 MEKLHQCYWK SGEPQSDDIE ASRMKRAAAK HLIERYYHQL TEGCGNEACT NEFCASCPTF 70 80 90 100 110 120 LRMDNNAAAI KALELYKINA KLCDPHPSKK GASSAYLENS KGAPNNSCSE IKMNKKGARI 130 140 150 160 170 180 DFKDVTYLTE EKVYEILELC REREDYSPLI RVIGRVFSSA EALVQSFRKV KQHTKEELKS 190 200 210 220 230 240 LQAKDEDKDE DEKEKAACSA AAMEEDSEAS SSRIGDSSQG DNNLQKLGPD DVSVDIDAIR 250 260 270 280 290 300 RVYTRLLSNE KIETAFLNAL VYLSPNVECD LTYHNVYSRD PNYLNLFIIV MENRNLHSPE 310 320 330 340 350 360 YLEMALPLFC KAMSKLPLAA QGKLIRLWSK YNADQIRRMM ETFQQLITYK VISNEFNSRN 370 380 390 400 410 420 LVNDDDAIVA ASKCLKMVYY ANVVGGEVDT NHNEEDDEEP IPESSELTLQ ELLGEERRNK 430 440 450 460 470 480 KGPRVDPLET ELGVKTLDCR KPLIPFEEFI NEPLNEVLEM DKDYTFFKVE TENKFSFMTC 490 500 510 520 530 540 PFILNAVTKN LGLYYDNRIR MYSERRITVL YSLVQGQQLN PYLRLKVRRD HIIDDALVRL 550 560 570 580 590 600 EMIAMENPAD LKKQLYVEFE GEQGVDEGGV SKEFFQLVVE EIFNPDIGMF TYDESTKLFW 610 620 630 640 650 660 FNPSSFETEG QFTLIGIVLG LAIYNNCILD VHFPMVVYRK LMGKKGTFRD LGDSHPVLYQ 670 680 690 700 710 720 SLKDLLEYEG NVEDDMMITF QISQTDLFGN PMMYDLKENG DKIPITNENR KEFVNLYSDY 730 740 750 760 770 780 ILNKSVEKQF KAFRRGFHMV TNESPLKYLF RPEEIELLIC GSRNLDFQAL EETTEYDGGY 790 800 810 820 830 840 TRDSVLIREF WEIVHSFTDE QKRLFLQFTT GTDRAPVGGL GKLKMIIAKN GPDTERLPTS 850 860 870 HTCFNVLLLP EYSSKEKLKE RLLKAITYAK GFGML
	Isoform I [UniParc]. Checksum: 3C061DA8D216055A		852	97,968
	10 20 30 40 50 60 MKRAAAKHLI ERYYHQLTEG CGNEACTNEF CASCPTFLRM DNNAAAIKAL ELYKINAKLC 70 80 90 100 110 120 DPHPSKKGAS SAYLENSKGA PNNSCSEIKM NKKGARIDFK DVTYLTEEKV YEILELCRER 130 140 150 160 170 180 EDYSPLIRVI GRVFSSAEAL VQSFRKVKQH TKEELKSLQA KDEDKDEDEK EKAACSAAAM 190 200 210 220 230 240 EEDSEASSSR IGDSSQGDNN LQKLGPDDVS VDIDAIRRVY TRLLSNEKIE TAFLNALVYL 250 260 270 280 290 300 SPNVECDLTY HNVYSRDPNY LNLFIIVMEN RNLHSPEYLE MALPLFCKAM SKLPLAAQGK 310 320 330 340 350 360 LIRLWSKYNA DQIRRMMETF QQLITYKVIS NEFNSRNLVN DDDAIVAASK CLKMVYYANV 370 380 390 400 410 420 VGGEVDTNHN EEDDEEPIPE SSELTLQELL GEERRNKKGP RVDPLETELG VKTLDCRKPL 430 440 450 460 470 480 IPFEEFINEP LNEVLEMDKD YTFFKVETEN KFSFMTCPFI LNAVTKNLGL YYDNRIRMYS 490 500 510 520 530 540 ERRITVLYSL VQGQQLNPYL RLKVRRDHII DDALVRLEMI AMENPADLKK QLYVEFEGEQ 550 560 570 580 590 600 GVDEGGVSKE FFQLVVEEIF NPDIGMFTYD ESTKLFWFNP SSFETEGQFT LIGIVLGLAI 610 620 630 640 650 660 YNNCILDVHF PMVVYRKLMG KKGTFRDLGD SHPVLYQSLK DLLEYEGNVE DDMMITFQIS 670 680 690 700 710 720 QTDLFGNPMM YDLKENGDKI PITNENRKEF VNLYSDYILN KSVEKQFKAF RRGFHMVTNE 730 740 750 760 770 780 SPLKYLFRPE EIELLICGSR NLDFQALEET TEYDGGYTRD SVLIREFWEI VHSFTDEQKR 790 800 810 820 830 840 LFLQFTTGTD RAPVGGLGKL KMIIAKNGPD TERLPTSHTC FNVLLLPEYS SKEKLKERLL 850 KAITYAKGFG ML
	Isoform III [UniParc]. Checksum: AB4C9B22356C9556		872	100,102
	10 20 30 40 50 60 MATACKRSGE PQSDDIEASR MKRAAAKHLI ERYYHQLTEG CGNEACTNEF CASCPTFLRM 70 80 90 100 110 120 DNNAAAIKAL ELYKINAKLC DPHPSKKGAS SAYLENSKGA PNNSCSEIKM NKKGARIDFK 130 140 150 160 170 180 DVTYLTEEKV YEILELCRER EDYSPLIRVI GRVFSSAEAL VQSFRKVKQH TKEELKSLQA 190 200 210 220 230 240 KDEDKDEDEK EKAACSAAAM EEDSEASSSR IGDSSQGDNN LQKLGPDDVS VDIDAIRRVY 250 260 270 280 290 300 TRLLSNEKIE TAFLNALVYL SPNVECDLTY HNVYSRDPNY LNLFIIVMEN RNLHSPEYLE 310 320 330 340 350 360 MALPLFCKAM SKLPLAAQGK LIRLWSKYNA DQIRRMMETF QQLITYKVIS NEFNSRNLVN 370 380 390 400 410 420 DDDAIVAASK CLKMVYYANV VGGEVDTNHN EEDDEEPIPE SSELTLQELL GEERRNKKGP 430 440 450 460 470 480 RVDPLETELG VKTLDCRKPL IPFEEFINEP LNEVLEMDKD YTFFKVETEN KFSFMTCPFI 490 500 510 520 530 540 LNAVTKNLGL YYDNRIRMYS ERRITVLYSL VQGQQLNPYL RLKVRRDHII DDALVRLEMI 550 560 570 580 590 600 AMENPADLKK QLYVEFEGEQ GVDEGGVSKE FFQLVVEEIF NPDIGMFTYD ESTKLFWFNP 610 620 630 640 650 660 SSFETEGQFT LIGIVLGLAI YNNCILDVHF PMVVYRKLMG KKGTFRDLGD SHPVLYQSLK 670 680 690 700 710 720 DLLEYEGNVE DDMMITFQIS QTDLFGNPMM YDLKENGDKI PITNENRKEF VNLYSDYILN 730 740 750 760 770 780 KSVEKQFKAF RRGFHMVTNE SPLKYLFRPE EIELLICGSR NLDFQALEET TEYDGGYTRD 790 800 810 820 830 840 SVLIREFWEI VHSFTDEQKR LFLQFTTGTD RAPVGGLGKL KMIIAKNGPD TERLPTSHTC 850 860 870 FNVLLLPEYS SKEKLKERLL KAITYAKGFG ML

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human E6-AP gene (UBE3A) encodes three potential protein isoforms generated by differential splicing." Yamamoto Y., Huibregtse J.M., Howley P.M. Genomics 41:263-266(1997) [PubMed: 9143503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS I; II AND III), VARIANT GLY-290. Tissue: Keratinocyte.
[2]	"UBE3A/E6-AP mutations cause Angelman syndrome." Kishino T., Lalande M., Wagstaff J. Nat. Genet. 15:70-73(1997) [PubMed: 8988171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM I). Tissue: Fetal brain.
[3]	"De novo truncating mutations in E6-AP ubiquitin-protein ligase gene (UBE3A) in Angelman syndrome." Matsuura T., Sutcliffe J.S., Fang P., Galjaard R.-J., Jiang Y.-H., Benton C.S., Rommens J.M., Beaudet A.L. Nat. Genet. 15:74-77(1997) [PubMed: 8988172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM I), VARIANTS TYR-44 AND THR-201.
[4]	"Analysis of the DNA sequence and duplication history of human chromosome 15." Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K., Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K., FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N., Abouelleil A. , Arachchi H.M., Baradarani L., Birditt B., Bloom S., Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K., DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J., Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E., Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B., Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R., O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B., Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S., Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C. Nature 440:671-675(2006) [PubMed: 16572171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Cloning and expression of the cDNA for E6-AP, a protein that mediates the interaction of the human papillomavirus E6 oncoprotein with p53." Huibregtse J.M., Scheffner M., Howley P.M.</