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Q05080

- CYK2_YEAST

UniProt

Q05080 - CYK2_YEAST

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Protein

Cytokinesis protein 2

Gene

HOF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Throughout most of the cell cycle it forms a double ring that coincides with the septins. After the onset of mitosis, forms a ring-like structure which colocalizes with the medial actin ring. Mediates cytoskeletal rearrangements required for cytokinesis. In conjunction with the medial actin ring exhibits contraction-like action.2 Publications

GO - Molecular functioni

  1. cytoskeletal protein binding Source: SGD

GO - Biological processi

  1. barrier septum assembly Source: SGD
  2. mitotic cytokinesis Source: SGD
  3. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32737-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytokinesis protein 2
Alternative name(s):
Homolog of CDC15 protein 1
Gene namesi
Name:HOF1
Synonyms:CYK2
Ordered Locus Names:YMR032W
ORF Names:YM9973.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR032w.
SGDiS000004635. HOF1.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck contractile ring Source: SGD
  2. cellular bud neck septin ring Source: SGD
  3. HICS complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 669669Cytokinesis protein 2PRO_0000079750Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei337 – 3371Phosphoserine1 Publication
Modified residuei366 – 3661Phosphoserine1 Publication
Modified residuei421 – 4211Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05080.
PaxDbiQ05080.
PeptideAtlasiQ05080.

Expressioni

Gene expression databases

GenevestigatoriQ05080.

Interactioni

Subunit structurei

Interacts with INN1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APP1P539332EBI-5412,EBI-28798
BNR1P404505EBI-5412,EBI-3711
INN1P539015EBI-5412,EBI-28955
LAS17Q124463EBI-5412,EBI-10022
SAP1P399554EBI-5412,EBI-16463
STP22P256042EBI-5412,EBI-411625
UBP5P399443EBI-5412,EBI-19847
UBP7P404532EBI-5412,EBI-19857
VRP1P373704EBI-5412,EBI-20502
YBP2P531692EBI-5412,EBI-23796

Protein-protein interaction databases

BioGridi35204. 411 interactions.
DIPiDIP-2729N.
IntActiQ05080. 55 interactions.
MINTiMINT-470371.
STRINGi4932.YMR032W.

Structurei

3D structure databases

ProteinModelPortaliQ05080.
SMRiQ05080. Positions 602-642.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 6969FCHPROSITE-ProRule annotationAdd
BLAST
Domaini599 – 66769SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili134 – 20067Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 FCH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG303196.
HOGENOMiHOG000112128.
InParanoidiQ05080.
OMAiFAKSWNS.
OrthoDBiEOG7F7WKS.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00611. FCH. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05080-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSYSYEACFW DPNDNGVNIL LGHISQGIRS CDSMILFFKQ RSELEKDYAR
60 70 80 90 100
RLGAITGKLD KDIGTNMDYG KLNETFNVVL SVEKARAQSH SKQSEILFRQ
110 120 130 140 150
IYTDTKAFAA NLQARYTTLS GKIERLRMDK FNKKKGCEVL QKKLQDAQIR
160 170 180 190 200
FRDLQLNENN MIGAKRVEHN KRELLKWESN SQEYKVQLDV LKQEYKASQK
210 220 230 240 250
FWIHEWAQLS CELQEMENAR ISFLQSKLQQ FATSSMETYI LEQTKMDMLT
260 270 280 290 300
NHLNSFTAAD EISTFSKENG TGRLKHKTSK GDMNSSANWA QMSSISTTSK
310 320 330 340 350
KTESYMDNIR KLSSQLKETE NKRKLASIDK YEKPLPSPEV TMATQFRNST
360 370 380 390 400
PVIRNETKVV ANPTLSLRSS PVQLQSNVDD SVLRQKPDKP RPIVGEEQLK
410 420 430 440 450
PDEDSKNPDE KGLMVHKRNQ SLSSPSESSS SNPTDFSHIK KRQSMESMTT
460 470 480 490 500
SVSSMANSID DSQRFAKSWN SSNRKRKSMS HLQVPSSASS RSDDGGRTPN
510 520 530 540 550
SAHNLNEDDY NTRRDTSTST ILFKPPVAVR GTSRGHTHRQ SMIMQDSSNP
560 570 580 590 600
IEDALYEMER IQSSSKPGTK TGNIMDERGV VRDRGITVTL PIVTSEGFPV
610 620 630 640 650
IEYAKAMYPL IGNEAPGLAN FHKGDYLLIT EIVNKDWYKG EVYDNDRIDR
660
NHRIGLIPYN FIQLLHQGL
Length:669
Mass (Da):76,207
Last modified:November 1, 1997 - v1
Checksum:iAE4A76C2F4043021
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49213 Genomic DNA. Translation: CAA89147.1.
BK006946 Genomic DNA. Translation: DAA09930.1.
PIRiS53948.
RefSeqiNP_013746.1. NM_001182529.1.

Genome annotation databases

EnsemblFungiiYMR032W; YMR032W; YMR032W.
GeneIDi855048.
KEGGisce:YMR032W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49213 Genomic DNA. Translation: CAA89147.1 .
BK006946 Genomic DNA. Translation: DAA09930.1 .
PIRi S53948.
RefSeqi NP_013746.1. NM_001182529.1.

3D structure databases

ProteinModelPortali Q05080.
SMRi Q05080. Positions 602-642.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35204. 411 interactions.
DIPi DIP-2729N.
IntActi Q05080. 55 interactions.
MINTi MINT-470371.
STRINGi 4932.YMR032W.

Proteomic databases

MaxQBi Q05080.
PaxDbi Q05080.
PeptideAtlasi Q05080.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR032W ; YMR032W ; YMR032W .
GeneIDi 855048.
KEGGi sce:YMR032W.

Organism-specific databases

CYGDi YMR032w.
SGDi S000004635. HOF1.

Phylogenomic databases

eggNOGi NOG303196.
HOGENOMi HOG000112128.
InParanoidi Q05080.
OMAi FAKSWNS.
OrthoDBi EOG7F7WKS.

Enzyme and pathway databases

BioCyci YEAST:G3O-32737-MONOMER.

Miscellaneous databases

NextBioi 978280.

Gene expression databases

Genevestigatori Q05080.

Family and domain databases

InterProi IPR001060. FCH_dom.
IPR001452. SH3_domain.
[Graphical view ]
Pfami PF00611. FCH. 1 hit.
[Graphical view ]
SMARTi SM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Dual function of Cyk2, a cdc15/PSTPIP family protein, in regulating actomyosin ring dynamics and septin distribution."
    Lippincott J., Li R.
    J. Cell Biol. 143:1947-1960(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast."
    Sanchez-Diaz A., Marchesi V., Murray S., Jones R., Pereira G., Edmondson R., Allen T., Labib K.
    Nat. Cell Biol. 10:395-406(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
  7. "Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae."
    Nishihama R., Schreiter J.H., Onishi M., Vallen E.A., Hanna J., Moravcevic K., Lippincott M.F., Han H., Lemmon M.A., Pringle J.R., Bi E.
    J. Cell Biol. 185:995-1012(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337; SER-366 AND SER-421, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCYK2_YEAST
AccessioniPrimary (citable) accession number: Q05080
Secondary accession number(s): D6VZK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3