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Protein

Cytokinesis protein 2

Gene

HOF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Throughout most of the cell cycle it forms a double ring that coincides with the septins. After the onset of mitosis, forms a ring-like structure which colocalizes with the medial actin ring. Mediates cytoskeletal rearrangements required for cytokinesis. In conjunction with the medial actin ring exhibits contraction-like action.2 Publications

GO - Molecular functioni

  • cytoskeletal protein binding Source: SGD

GO - Biological processi

  • barrier septum assembly Source: SGD
  • mitotic cytokinesis Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of formin-nucleated actin cable assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-32737-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytokinesis protein 2
Alternative name(s):
Homolog of CDC15 protein 1
Gene namesi
Name:HOF1
Synonyms:CYK2
Ordered Locus Names:YMR032W
ORF Names:YM9973.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR032W.
SGDiS000004635. HOF1.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud neck contractile ring Source: SGD
  • cellular bud neck septin ring Source: SGD
  • HICS complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000797501 – 669Cytokinesis protein 2Add BLAST669

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei337PhosphoserineCombined sources1
Modified residuei366PhosphoserineCombined sources1
Modified residuei421PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05080.
PRIDEiQ05080.

PTM databases

iPTMnetiQ05080.

Interactioni

Subunit structurei

Interacts with INN1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APP1P539332EBI-5412,EBI-28798
BNR1P404505EBI-5412,EBI-3711
INN1P539015EBI-5412,EBI-28955
LAS17Q124463EBI-5412,EBI-10022
SAP1P399554EBI-5412,EBI-16463
STP22P256042EBI-5412,EBI-411625
UBP5P399443EBI-5412,EBI-19847
UBP7P404532EBI-5412,EBI-19857
VRP1P373704EBI-5412,EBI-20502
YBP2P531692EBI-5412,EBI-23796

GO - Molecular functioni

  • cytoskeletal protein binding Source: SGD

Protein-protein interaction databases

BioGridi35204. 424 interactors.
DIPiDIP-2729N.
IntActiQ05080. 55 interactors.
MINTiMINT-470371.

Structurei

Secondary structure

1669
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 8Combined sources4
Helixi15 – 29Combined sources15
Helixi31 – 65Combined sources35
Helixi70 – 99Combined sources30
Helixi102 – 156Combined sources55
Helixi158 – 160Combined sources3
Helixi164 – 253Combined sources90
Helixi258 – 269Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WPEX-ray2.70A2-300[»]
ProteinModelPortaliQ05080.
SMRiQ05080.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 261F-BARPROSITE-ProRule annotationAdd BLAST261
Domaini599 – 667SH3PROSITE-ProRule annotationAdd BLAST69

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili134 – 200Sequence analysisAdd BLAST67

Sequence similaritiesi

Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

HOGENOMiHOG000112128.
InParanoidiQ05080.
OMAiFAKSWNS.
OrthoDBiEOG092C3EOZ.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00611. FCH. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05080-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYSYEACFW DPNDNGVNIL LGHISQGIRS CDSMILFFKQ RSELEKDYAR
60 70 80 90 100
RLGAITGKLD KDIGTNMDYG KLNETFNVVL SVEKARAQSH SKQSEILFRQ
110 120 130 140 150
IYTDTKAFAA NLQARYTTLS GKIERLRMDK FNKKKGCEVL QKKLQDAQIR
160 170 180 190 200
FRDLQLNENN MIGAKRVEHN KRELLKWESN SQEYKVQLDV LKQEYKASQK
210 220 230 240 250
FWIHEWAQLS CELQEMENAR ISFLQSKLQQ FATSSMETYI LEQTKMDMLT
260 270 280 290 300
NHLNSFTAAD EISTFSKENG TGRLKHKTSK GDMNSSANWA QMSSISTTSK
310 320 330 340 350
KTESYMDNIR KLSSQLKETE NKRKLASIDK YEKPLPSPEV TMATQFRNST
360 370 380 390 400
PVIRNETKVV ANPTLSLRSS PVQLQSNVDD SVLRQKPDKP RPIVGEEQLK
410 420 430 440 450
PDEDSKNPDE KGLMVHKRNQ SLSSPSESSS SNPTDFSHIK KRQSMESMTT
460 470 480 490 500
SVSSMANSID DSQRFAKSWN SSNRKRKSMS HLQVPSSASS RSDDGGRTPN
510 520 530 540 550
SAHNLNEDDY NTRRDTSTST ILFKPPVAVR GTSRGHTHRQ SMIMQDSSNP
560 570 580 590 600
IEDALYEMER IQSSSKPGTK TGNIMDERGV VRDRGITVTL PIVTSEGFPV
610 620 630 640 650
IEYAKAMYPL IGNEAPGLAN FHKGDYLLIT EIVNKDWYKG EVYDNDRIDR
660
NHRIGLIPYN FIQLLHQGL
Length:669
Mass (Da):76,207
Last modified:November 1, 1997 - v1
Checksum:iAE4A76C2F4043021
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49213 Genomic DNA. Translation: CAA89147.1.
BK006946 Genomic DNA. Translation: DAA09930.1.
PIRiS53948.
RefSeqiNP_013746.1. NM_001182529.1.

Genome annotation databases

EnsemblFungiiYMR032W; YMR032W; YMR032W.
GeneIDi855048.
KEGGisce:YMR032W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49213 Genomic DNA. Translation: CAA89147.1.
BK006946 Genomic DNA. Translation: DAA09930.1.
PIRiS53948.
RefSeqiNP_013746.1. NM_001182529.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4WPEX-ray2.70A2-300[»]
ProteinModelPortaliQ05080.
SMRiQ05080.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35204. 424 interactors.
DIPiDIP-2729N.
IntActiQ05080. 55 interactors.
MINTiMINT-470371.

PTM databases

iPTMnetiQ05080.

Proteomic databases

MaxQBiQ05080.
PRIDEiQ05080.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR032W; YMR032W; YMR032W.
GeneIDi855048.
KEGGisce:YMR032W.

Organism-specific databases

EuPathDBiFungiDB:YMR032W.
SGDiS000004635. HOF1.

Phylogenomic databases

HOGENOMiHOG000112128.
InParanoidiQ05080.
OMAiFAKSWNS.
OrthoDBiEOG092C3EOZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-32737-MONOMER.

Miscellaneous databases

PROiQ05080.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00611. FCH. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYK2_YEAST
AccessioniPrimary (citable) accession number: Q05080
Secondary accession number(s): D6VZK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.