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Q05080 (CYK2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytokinesis protein 2
Alternative name(s):
Homolog of CDC15 protein 1
Gene names
Name:HOF1
Synonyms:CYK2
Ordered Locus Names:YMR032W
ORF Names:YM9973.05
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length669 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Throughout most of the cell cycle it forms a double ring that coincides with the septins. After the onset of mitosis, forms a ring-like structure which colocalizes with the medial actin ring. Mediates cytoskeletal rearrangements required for cytokinesis. In conjunction with the medial actin ring exhibits contraction-like action. Ref.6 Ref.7

Subunit structure

Interacts with INN1. Ref.6 Ref.7

Subcellular location

Cytoplasmcytoskeleton. Bud neck Ref.6 Ref.7.

Miscellaneous

Present with 195 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 FCH domain.

Contains 1 SH3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 669669Cytokinesis protein 2
PRO_0000079750

Regions

Domain1 – 6969FCH
Domain599 – 66769SH3
Coiled coil134 – 20067 Potential

Amino acid modifications

Modified residue3371Phosphoserine Ref.8
Modified residue3661Phosphoserine Ref.8
Modified residue4211Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
Q05080 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: AE4A76C2F4043021

FASTA66976,207
        10         20         30         40         50         60 
MSYSYEACFW DPNDNGVNIL LGHISQGIRS CDSMILFFKQ RSELEKDYAR RLGAITGKLD 

        70         80         90        100        110        120 
KDIGTNMDYG KLNETFNVVL SVEKARAQSH SKQSEILFRQ IYTDTKAFAA NLQARYTTLS 

       130        140        150        160        170        180 
GKIERLRMDK FNKKKGCEVL QKKLQDAQIR FRDLQLNENN MIGAKRVEHN KRELLKWESN 

       190        200        210        220        230        240 
SQEYKVQLDV LKQEYKASQK FWIHEWAQLS CELQEMENAR ISFLQSKLQQ FATSSMETYI 

       250        260        270        280        290        300 
LEQTKMDMLT NHLNSFTAAD EISTFSKENG TGRLKHKTSK GDMNSSANWA QMSSISTTSK 

       310        320        330        340        350        360 
KTESYMDNIR KLSSQLKETE NKRKLASIDK YEKPLPSPEV TMATQFRNST PVIRNETKVV 

       370        380        390        400        410        420 
ANPTLSLRSS PVQLQSNVDD SVLRQKPDKP RPIVGEEQLK PDEDSKNPDE KGLMVHKRNQ 

       430        440        450        460        470        480 
SLSSPSESSS SNPTDFSHIK KRQSMESMTT SVSSMANSID DSQRFAKSWN SSNRKRKSMS 

       490        500        510        520        530        540 
HLQVPSSASS RSDDGGRTPN SAHNLNEDDY NTRRDTSTST ILFKPPVAVR GTSRGHTHRQ 

       550        560        570        580        590        600 
SMIMQDSSNP IEDALYEMER IQSSSKPGTK TGNIMDERGV VRDRGITVTL PIVTSEGFPV 

       610        620        630        640        650        660 
IEYAKAMYPL IGNEAPGLAN FHKGDYLLIT EIVNKDWYKG EVYDNDRIDR NHRIGLIPYN 


FIQLLHQGL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Dual function of Cyk2, a cdc15/PSTPIP family protein, in regulating actomyosin ring dynamics and septin distribution."
Lippincott J., Li R.
J. Cell Biol. 143:1947-1960(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast."
Sanchez-Diaz A., Marchesi V., Murray S., Jones R., Pereira G., Edmondson R., Allen T., Labib K.
Nat. Cell Biol. 10:395-406(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
[7]"Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae."
Nishihama R., Schreiter J.H., Onishi M., Vallen E.A., Hanna J., Moravcevic K., Lippincott M.F., Han H., Lemmon M.A., Pringle J.R., Bi E.
J. Cell Biol. 185:995-1012(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337; SER-366 AND SER-421, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49213 Genomic DNA. Translation: CAA89147.1.
BK006946 Genomic DNA. Translation: DAA09930.1.
PIRS53948.
RefSeqNP_013746.1. NM_001182529.1.

3D structure databases

ProteinModelPortalQ05080.
SMRQ05080. Positions 602-642.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35204. 410 interactions.
DIPDIP-2729N.
IntActQ05080. 55 interactions.
MINTMINT-470371.
STRING4932.YMR032W.

Proteomic databases

PaxDbQ05080.
PeptideAtlasQ05080.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR032W; YMR032W; YMR032W.
GeneID855048.
KEGGsce:YMR032W.

Organism-specific databases

CYGDYMR032w.
SGDS000004635. HOF1.

Phylogenomic databases

eggNOGNOG303196.
HOGENOMHOG000112128.
OMAFAKSWNS.
OrthoDBEOG7F7WKS.

Enzyme and pathway databases

BioCycYEAST:G3O-32737-MONOMER.

Gene expression databases

GenevestigatorQ05080.

Family and domain databases

InterProIPR001060. FCH_dom.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00611. FCH. 1 hit.
[Graphical view]
SMARTSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978280.

Entry information

Entry nameCYK2_YEAST
AccessionPrimary (citable) accession number: Q05080
Secondary accession number(s): D6VZK6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families