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Q05080

- CYK2_YEAST

UniProt

Q05080 - CYK2_YEAST

Protein

Cytokinesis protein 2

Gene

HOF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Throughout most of the cell cycle it forms a double ring that coincides with the septins. After the onset of mitosis, forms a ring-like structure which colocalizes with the medial actin ring. Mediates cytoskeletal rearrangements required for cytokinesis. In conjunction with the medial actin ring exhibits contraction-like action.2 Publications

    GO - Molecular functioni

    1. cytoskeletal protein binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. barrier septum assembly Source: SGD
    2. mitotic cytokinesis Source: SGD
    3. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32737-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytokinesis protein 2
    Alternative name(s):
    Homolog of CDC15 protein 1
    Gene namesi
    Name:HOF1
    Synonyms:CYK2
    Ordered Locus Names:YMR032W
    ORF Names:YM9973.05
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR032w.
    SGDiS000004635. HOF1.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck contractile ring Source: SGD
    2. cellular bud neck septin ring Source: SGD
    3. HICS complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 669669Cytokinesis protein 2PRO_0000079750Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei337 – 3371Phosphoserine1 Publication
    Modified residuei366 – 3661Phosphoserine1 Publication
    Modified residuei421 – 4211Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ05080.
    PaxDbiQ05080.
    PeptideAtlasiQ05080.

    Expressioni

    Gene expression databases

    GenevestigatoriQ05080.

    Interactioni

    Subunit structurei

    Interacts with INN1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APP1P539332EBI-5412,EBI-28798
    BNR1P404505EBI-5412,EBI-3711
    INN1P539015EBI-5412,EBI-28955
    LAS17Q124463EBI-5412,EBI-10022
    SAP1P399554EBI-5412,EBI-16463
    STP22P256042EBI-5412,EBI-411625
    UBP5P399443EBI-5412,EBI-19847
    UBP7P404532EBI-5412,EBI-19857
    VRP1P373704EBI-5412,EBI-20502
    YBP2P531692EBI-5412,EBI-23796

    Protein-protein interaction databases

    BioGridi35204. 410 interactions.
    DIPiDIP-2729N.
    IntActiQ05080. 55 interactions.
    MINTiMINT-470371.
    STRINGi4932.YMR032W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05080.
    SMRiQ05080. Positions 602-642.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 6969FCHPROSITE-ProRule annotationAdd
    BLAST
    Domaini599 – 66769SH3PROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili134 – 20067Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FCH domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    eggNOGiNOG303196.
    HOGENOMiHOG000112128.
    OMAiFAKSWNS.
    OrthoDBiEOG7F7WKS.

    Family and domain databases

    InterProiIPR001060. FCH_dom.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00611. FCH. 1 hit.
    [Graphical view]
    SMARTiSM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q05080-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYSYEACFW DPNDNGVNIL LGHISQGIRS CDSMILFFKQ RSELEKDYAR    50
    RLGAITGKLD KDIGTNMDYG KLNETFNVVL SVEKARAQSH SKQSEILFRQ 100
    IYTDTKAFAA NLQARYTTLS GKIERLRMDK FNKKKGCEVL QKKLQDAQIR 150
    FRDLQLNENN MIGAKRVEHN KRELLKWESN SQEYKVQLDV LKQEYKASQK 200
    FWIHEWAQLS CELQEMENAR ISFLQSKLQQ FATSSMETYI LEQTKMDMLT 250
    NHLNSFTAAD EISTFSKENG TGRLKHKTSK GDMNSSANWA QMSSISTTSK 300
    KTESYMDNIR KLSSQLKETE NKRKLASIDK YEKPLPSPEV TMATQFRNST 350
    PVIRNETKVV ANPTLSLRSS PVQLQSNVDD SVLRQKPDKP RPIVGEEQLK 400
    PDEDSKNPDE KGLMVHKRNQ SLSSPSESSS SNPTDFSHIK KRQSMESMTT 450
    SVSSMANSID DSQRFAKSWN SSNRKRKSMS HLQVPSSASS RSDDGGRTPN 500
    SAHNLNEDDY NTRRDTSTST ILFKPPVAVR GTSRGHTHRQ SMIMQDSSNP 550
    IEDALYEMER IQSSSKPGTK TGNIMDERGV VRDRGITVTL PIVTSEGFPV 600
    IEYAKAMYPL IGNEAPGLAN FHKGDYLLIT EIVNKDWYKG EVYDNDRIDR 650
    NHRIGLIPYN FIQLLHQGL 669
    Length:669
    Mass (Da):76,207
    Last modified:November 1, 1997 - v1
    Checksum:iAE4A76C2F4043021
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49213 Genomic DNA. Translation: CAA89147.1.
    BK006946 Genomic DNA. Translation: DAA09930.1.
    PIRiS53948.
    RefSeqiNP_013746.1. NM_001182529.1.

    Genome annotation databases

    EnsemblFungiiYMR032W; YMR032W; YMR032W.
    GeneIDi855048.
    KEGGisce:YMR032W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49213 Genomic DNA. Translation: CAA89147.1 .
    BK006946 Genomic DNA. Translation: DAA09930.1 .
    PIRi S53948.
    RefSeqi NP_013746.1. NM_001182529.1.

    3D structure databases

    ProteinModelPortali Q05080.
    SMRi Q05080. Positions 602-642.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35204. 410 interactions.
    DIPi DIP-2729N.
    IntActi Q05080. 55 interactions.
    MINTi MINT-470371.
    STRINGi 4932.YMR032W.

    Proteomic databases

    MaxQBi Q05080.
    PaxDbi Q05080.
    PeptideAtlasi Q05080.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR032W ; YMR032W ; YMR032W .
    GeneIDi 855048.
    KEGGi sce:YMR032W.

    Organism-specific databases

    CYGDi YMR032w.
    SGDi S000004635. HOF1.

    Phylogenomic databases

    eggNOGi NOG303196.
    HOGENOMi HOG000112128.
    OMAi FAKSWNS.
    OrthoDBi EOG7F7WKS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32737-MONOMER.

    Miscellaneous databases

    NextBioi 978280.

    Gene expression databases

    Genevestigatori Q05080.

    Family and domain databases

    InterProi IPR001060. FCH_dom.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00611. FCH. 1 hit.
    [Graphical view ]
    SMARTi SM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Dual function of Cyk2, a cdc15/PSTPIP family protein, in regulating actomyosin ring dynamics and septin distribution."
      Lippincott J., Li R.
      J. Cell Biol. 143:1947-1960(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast."
      Sanchez-Diaz A., Marchesi V., Murray S., Jones R., Pereira G., Edmondson R., Allen T., Labib K.
      Nat. Cell Biol. 10:395-406(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
    7. "Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae."
      Nishihama R., Schreiter J.H., Onishi M., Vallen E.A., Hanna J., Moravcevic K., Lippincott M.F., Han H., Lemmon M.A., Pringle J.R., Bi E.
      J. Cell Biol. 185:995-1012(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-337; SER-366 AND SER-421, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCYK2_YEAST
    AccessioniPrimary (citable) accession number: Q05080
    Secondary accession number(s): D6VZK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 195 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3