Q05080 (CYK2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytokinesis protein 2

Alternative name(s):
Homolog of CDC15 protein 1

Gene names

Name:	HOF1
Synonyms:	CYK2
Ordered Locus Names:	YMR032W
ORF Names:	YM9973.05

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Throughout most of the cell cycle it forms a double ring that coincides with the septins. After the onset of mitosis, forms a ring-like structure which colocalizes with the medial actin ring. Mediates cytoskeletal rearrangements required for cytokinesis. In conjunction with the medial actin ring exhibits contraction-like action. Ref.6 Ref.7
Subunit structure	Interacts with INN1. Ref.6 Ref.7
Subcellular location	Cytoplasm › cytoskeleton. Bud neck Ref.6 Ref.7.
Miscellaneous	Present with 195 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 FCH domain. Contains 1 SH3 domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton
Domain	Coiled coil SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	barrier septum assembly involved in cell cycle cytokinesis Inferred from mutant phenotype PubMed 21498574. Source: SGD cytokinesis after mitosis Inferred from mutant phenotype PubMed 9774458Ref.3. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW response to DNA damage stimulus Inferred from mutant phenotype PubMed 12482937. Source: SGD
Cellular_component	HICS complex Inferred from physical interaction PubMed 22623719. Source: SGD cellular bud neck contractile ring Inferred from direct assay PubMed 15775961Ref.3. Source: SGD cellular bud neck septin ring Inferred from direct assay Ref.3. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BNR1	P40450	3	EBI-5412,EBI-3711
INN1	P53901	3	EBI-5412,EBI-28955

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 669	669	Cytokinesis protein 2		PRO_0000079750
Regions
	Domain	1 – 69	69	FCH
	Domain	599 – 667	69	SH3
	Coiled coil	134 – 200	67	Potential
Amino acid modifications
	Modified residue	370	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q05080 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: AE4A76C2F4043021	FASTA	669	76,207
	10 20 30 40 50 60 MSYSYEACFW DPNDNGVNIL LGHISQGIRS CDSMILFFKQ RSELEKDYAR RLGAITGKLD 70 80 90 100 110 120 KDIGTNMDYG KLNETFNVVL SVEKARAQSH SKQSEILFRQ IYTDTKAFAA NLQARYTTLS 130 140 150 160 170 180 GKIERLRMDK FNKKKGCEVL QKKLQDAQIR FRDLQLNENN MIGAKRVEHN KRELLKWESN 190 200 210 220 230 240 SQEYKVQLDV LKQEYKASQK FWIHEWAQLS CELQEMENAR ISFLQSKLQQ FATSSMETYI 250 260 270 280 290 300 LEQTKMDMLT NHLNSFTAAD EISTFSKENG TGRLKHKTSK GDMNSSANWA QMSSISTTSK 310 320 330 340 350 360 KTESYMDNIR KLSSQLKETE NKRKLASIDK YEKPLPSPEV TMATQFRNST PVIRNETKVV 370 380 390 400 410 420 ANPTLSLRSS PVQLQSNVDD SVLRQKPDKP RPIVGEEQLK PDEDSKNPDE KGLMVHKRNQ 430 440 450 460 470 480 SLSSPSESSS SNPTDFSHIK KRQSMESMTT SVSSMANSID DSQRFAKSWN SSNRKRKSMS 490 500 510 520 530 540 HLQVPSSASS RSDDGGRTPN SAHNLNEDDY NTRRDTSTST ILFKPPVAVR GTSRGHTHRQ 550 560 570 580 590 600 SMIMQDSSNP IEDALYEMER IQSSSKPGTK TGNIMDERGV VRDRGITVTL PIVTSEGFPV 610 620 630 640 650 660 IEYAKAMYPL IGNEAPGLAN FHKGDYLLIT EIVNKDWYKG EVYDNDRIDR NHRIGLIPYN FIQLLHQGL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Dual function of Cyk2, a cdc15/PSTPIP family protein, in regulating actomyosin ring dynamics and septin distribution." Lippincott J., Li R. J. Cell Biol. 143:1947-1960(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-370, MASS SPECTROMETRY.
[6]	"Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast." Sanchez-Diaz A., Marchesi V., Murray S., Jones R., Pereira G., Edmondson R., Allen T., Labib K. Nat. Cell Biol. 10:395-406(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
[7]	"Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae." Nishihama R., Schreiter J.H., Onishi M., Vallen E.A., Hanna J., Moravcevic K., Lippincott M.F., Han H., Lemmon M.A., Pringle J.R., Bi E. J. Cell Biol. 185:995-1012(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49213 Genomic DNA. Translation: CAA89147.1. BK006946 Genomic DNA. Translation: DAA09930.1.
PIR	S53948.
RefSeq	NP_013746.1. NM_001182529.1.
3D structure databases
ProteinModelPortal	Q05080.
SMR	Q05080. Positions 602-642.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2729N.
IntAct	Q05080. 19 interactions.
MINT	MINT-470371.
STRING	4932.YMR032W.
Proteomic databases
PaxDb	Q05080.
PeptideAtlas	Q05080.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR032W; YMR032W; YMR032W.
GeneID	855048.
KEGG	sce:YMR032W.
Organism-specific databases
CYGD	YMR032w.
SGD	S000004635. HOF1.
Phylogenomic databases
eggNOG	NOG303196.
HOGENOM	HOG000112128.
OMA	FAKSWNS.
OrthoDB	EOG4N07PQ.
Gene expression databases
Genevestigator	Q05080.
GermOnline	YMR032W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001060. FCH_dom. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00611. FCH. 1 hit. [Graphical view]
SMART	SM00055. FCH. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS50133. FCH. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978280.

Entry information

Entry name

CYK2_YEAST

Accession

Primary (citable) accession number: Q05080
Secondary accession number(s): D6VZK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program