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Protein

Fructose-1,6-bisphosphatase

Gene

FBP1

Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Subject to complex allosteric regulation. The enzyme can assume an active R-state, or an inactive T-state. Intermediate conformations may exist. AMP acts as allosteric inhibitor. AMP binding affects the turnover of bound substrate and not the affinity for substrate (By similarity).By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi78 – 781Magnesium 1By similarity
Metal bindingi107 – 1071Magnesium 1By similarity
Metal bindingi107 – 1071Magnesium 2By similarity
Metal bindingi127 – 1271Magnesium 2By similarity
Metal bindingi127 – 1271Magnesium 3By similarity
Metal bindingi129 – 1291Magnesium 2; via carbonyl oxygenBy similarity
Metal bindingi130 – 1301Magnesium 3By similarity
Binding sitei230 – 2301SubstrateBy similarity
Binding sitei264 – 2641SubstrateBy similarity
Binding sitei284 – 2841SubstrateBy similarity
Binding sitei294 – 2941SubstrateBy similarity
Metal bindingi300 – 3001Magnesium 3By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 415AMPBy similarity
Nucleotide bindingi121 – 1222AMPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-1,6-bisphosphatase (EC:3.1.3.11)
Short name:
FBPase
Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolase
Gene namesi
Name:FBP1
Ordered Locus Names:KLLA0E01210g
OrganismiKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica)
Taxonomic identifieri284590 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces
Proteomesi
  • UP000000598 Componenti: Chromosome E

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCurated
Chaini2 – 355354Fructose-1,6-bisphosphatasePRO_0000200509Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi284590.XP_454003.1.

Structurei

3D structure databases

ProteinModelPortaliQ05079.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni130 – 1334Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the FBPase class 1 family.Curated

Phylogenomic databases

eggNOGiKOG1458. Eukaryota.
COG0158. LUCA.
HOGENOMiHOG000191265.
InParanoidiQ05079.
KOiK03841.
OMAiGTIFAIH.
OrthoDBiEOG092C350K.

Family and domain databases

CDDicd00354. FBPase. 1 hit.
HAMAPiMF_01855. FBPase_class1. 1 hit.
InterProiIPR000146. FBPase_class-1.
IPR033391. FBPase_N.
IPR028343. FBPtase.
IPR020548. Fructose_bisphosphatase_AS.
[Graphical view]
PANTHERiPTHR11556. PTHR11556. 1 hit.
PfamiPF00316. FBPase. 1 hit.
[Graphical view]
PIRSFiPIRSF500210. FBPtase. 1 hit.
PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTSiPR00115. F16BPHPHTASE.
PROSITEiPS00124. FBPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05079-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGIKHRRDS AESINTDIIT LSRFILDQQH LSAKNATGEF SMLLNSLQFA
60 70 80 90 100
FKFISQTIRR AELVNLIGLA GASNSTGDQQ KKLDVLGDEI FINAMKASGN
110 120 130 140 150
VKVLVSEEQE DLIVFRNSPG KYAVCCDPID GSSNLDAGVS VGTIVSLFKI
160 170 180 190 200
HENQNGNSGE EDSEGTINDV ARCGREMVAA CYTMYGASTH LVLTTGAGVN
210 220 230 240 250
GFTLDNNLGE FILTYPELRL PEQKSIYSIN EGNTCYWEPT IADFIAKLKE
260 270 280 290 300
NSEENNGKPY SARYIGSMVA DVHRTLLYGG LFSYPGDKKN PNGKLRLLYE
310 320 330 340 350
AFPMAFLVEQ AGGKAVNDRG ERILDLVPQH IHDKSSIWLG SSGDVDKYLK

HIGKL
Length:355
Mass (Da):38,899
Last modified:January 23, 2007 - v2
Checksum:i3E24B69D32980AF6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70181 Genomic DNA. Translation: CAA49728.1.
CR382125 Genomic DNA. Translation: CAG99090.1.
PIRiS29397.
RefSeqiXP_454003.1. XM_454003.1.

Genome annotation databases

EnsemblFungiiCAG99090; CAG99090; KLLA0_E01211g.
GeneIDi2894286.
KEGGikla:KLLA0E01211g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70181 Genomic DNA. Translation: CAA49728.1.
CR382125 Genomic DNA. Translation: CAG99090.1.
PIRiS29397.
RefSeqiXP_454003.1. XM_454003.1.

3D structure databases

ProteinModelPortaliQ05079.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi284590.XP_454003.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG99090; CAG99090; KLLA0_E01211g.
GeneIDi2894286.
KEGGikla:KLLA0E01211g.

Phylogenomic databases

eggNOGiKOG1458. Eukaryota.
COG0158. LUCA.
HOGENOMiHOG000191265.
InParanoidiQ05079.
KOiK03841.
OMAiGTIFAIH.
OrthoDBiEOG092C350K.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

CDDicd00354. FBPase. 1 hit.
HAMAPiMF_01855. FBPase_class1. 1 hit.
InterProiIPR000146. FBPase_class-1.
IPR033391. FBPase_N.
IPR028343. FBPtase.
IPR020548. Fructose_bisphosphatase_AS.
[Graphical view]
PANTHERiPTHR11556. PTHR11556. 1 hit.
PfamiPF00316. FBPase. 1 hit.
[Graphical view]
PIRSFiPIRSF500210. FBPtase. 1 hit.
PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTSiPR00115. F16BPHPHTASE.
PROSITEiPS00124. FBPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiF16P_KLULA
AccessioniPrimary (citable) accession number: Q05079
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.