Q05069 (FABI_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
Short name=ENR
EC=1.3.1.9

Alternative name(s):
NADH-dependent enoyl-ACP reductase

Gene names

Name:	fabI
Ordered Locus Names:	all4391

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism By similarity.
Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.
Sequence caution	The sequence AAD04184.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAB76090.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid elongation Inferred from sequence or structural similarity. Source: UniProtKB protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from sequence or structural similarity. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Enoyl-[acyl-carrier-protein] reductase [NADH] FabI		PRO_0000054893
Regions
	Nucleotide binding	19 – 20	2	NAD By similarity
	Nucleotide binding	67 – 68	2	NAD By similarity
	Nucleotide binding	194 – 198	5	NAD By similarity
Sites
	Active site	148	1	Proton acceptor By similarity
	Active site	158	1	Proton acceptor By similarity
	Binding site	13	1	NAD; via carbonyl oxygen By similarity
	Binding site	95	1	NAD; via carbonyl oxygen By similarity
	Binding site	98	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	165	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05069 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 45012CA8497E001B	FASTA	258	27,421
	10 20 30 40 50 60 MLNLTGKNAL VTGIANNRSI AWGIAQQLHA AGANLGITYL PDERGKFEKK VSELVEPLNP 70 80 90 100 110 120 SLFLPCNVQN DEQIQSTFDT IRDKWGRLDI LIHCLAFANR DDLTGDFSQT SRAGFATALD 130 140 150 160 170 180 ISTFSLVQLS GAAKPLMTEG GSIITLSYLG GVRAVPNYNV MGVAKAGLEA SVRYLASELG 190 200 210 220 230 240 SQNIRVNAIS AGPIRTLASS AVGGILDMIH HVEQVAPLRR TVTQLEVGNT AAFLASDLAS 250 GITGQVLYVD AGYEIMGM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection." Wei T.-F., Ramasubramanian T.S., Pu F., Golden J.W. J. Bacteriol. 175:4025-4035(1993) [PubMed: 8391534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10036 Genomic DNA. Translation: AAD04184.1. Different initiation. BA000019 Genomic DNA. Translation: BAB76090.1. Different initiation.
PIR	AG2354.
RefSeq	NP_488431.1. NC_003272.1.
3D structure databases
ProteinModelPortal	Q05069.
ModBase	Search...
Protein-protein interaction databases
STRING	Q05069.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1107992.
GenomeReviews	Gene locus all4391 in contig BA000019_GR.
KEGG	ana:all4391.
PATRIC	22779366. VBINosSp37423_5130.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0623.
HOGENOM	HBG750976.
OMA	MGVANNR.
PhylomeDB	Q05069.
ProtClustDB	PRK07370.
Enzyme and pathway databases
BioCyc	NSP103690:ALL4391-MONOMER.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00208.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PIRSF	PIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

FABI_NOSS1

Accession

Primary (citable) accession number: Q05069

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program