Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13NAD; via carbonyl oxygenBy similarity1
Binding sitei95NAD; via carbonyl oxygenBy similarity1
Binding sitei98Substrate; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei148Proton acceptorBy similarity1
Active sitei158Proton acceptorBy similarity1
Binding sitei165NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 20NADBy similarity2
Nucleotide bindingi67 – 68NADBy similarity2
Nucleotide bindingi194 – 198NADBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:all4391
OrganismiNostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000002483 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000548931 – 258Enoyl-[acyl-carrier-protein] reductase [NADH] FabIAdd BLAST258

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi103690.all4391.

Structurei

3D structure databases

ProteinModelPortaliQ05069.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CSJ. Bacteria.
COG0623. LUCA.
KOiK00208.
OMAiDILIHCL.
OrthoDBiPOG091H06HU.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Q05069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNLTGKNAL VTGIANNRSI AWGIAQQLHA AGANLGITYL PDERGKFEKK
60 70 80 90 100
VSELVEPLNP SLFLPCNVQN DEQIQSTFDT IRDKWGRLDI LIHCLAFANR
110 120 130 140 150
DDLTGDFSQT SRAGFATALD ISTFSLVQLS GAAKPLMTEG GSIITLSYLG
160 170 180 190 200
GVRAVPNYNV MGVAKAGLEA SVRYLASELG SQNIRVNAIS AGPIRTLASS
210 220 230 240 250
AVGGILDMIH HVEQVAPLRR TVTQLEVGNT AAFLASDLAS GITGQVLYVD

AGYEIMGM
Length:258
Mass (Da):27,421
Last modified:May 30, 2000 - v2
Checksum:i45012CA8497E001B
GO

Sequence cautioni

The sequence AAD04184 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAB76090 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10036 Genomic DNA. Translation: AAD04184.1. Different initiation.
BA000019 Genomic DNA. Translation: BAB76090.1. Different initiation.
PIRiAG2354.
RefSeqiWP_041456288.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB76090; BAB76090; BAB76090.
KEGGiana:all4391.
PATRICi22779366. VBINosSp37423_5130.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10036 Genomic DNA. Translation: AAD04184.1. Different initiation.
BA000019 Genomic DNA. Translation: BAB76090.1. Different initiation.
PIRiAG2354.
RefSeqiWP_041456288.1. NC_003272.1.

3D structure databases

ProteinModelPortaliQ05069.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi103690.all4391.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB76090; BAB76090; BAB76090.
KEGGiana:all4391.
PATRICi22779366. VBINosSp37423_5130.

Phylogenomic databases

eggNOGiENOG4105CSJ. Bacteria.
COG0623. LUCA.
KOiK00208.
OMAiDILIHCL.
OrthoDBiPOG091H06HU.

Enzyme and pathway databases

UniPathwayiUPA00094.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFABI_NOSS1
AccessioniPrimary (citable) accession number: Q05069
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: May 30, 2000
Last modified: September 7, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.