Reviewed, UniProtKB/Swiss-Prot Q05069 (FABI_ANASP)

Names and origin

Protein names

Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH]
    EC=1.3.1.9
Alternative name(s):
    NADH-dependent enoyl-ACP reductase

Gene names

Name:	fabI
Ordered Locus Names:	all4391

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Enoyl-[acyl-carrier-protein] reductase [NADH]		PRO_0000054893
Regions
	Nucleotide binding	10 – 36	27	NAD By similarity
Sites
	Active site	158	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05069-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 45012CA8497E001B	FASTA	258	27,421
	10 20 30 40 50 60 MLNLTGKNAL VTGIANNRSI AWGIAQQLHA AGANLGITYL PDERGKFEKK VSELVEPLNP 70 80 90 100 110 120 SLFLPCNVQN DEQIQSTFDT IRDKWGRLDI LIHCLAFANR DDLTGDFSQT SRAGFATALD 130 140 150 160 170 180 ISTFSLVQLS GAAKPLMTEG GSIITLSYLG GVRAVPNYNV MGVAKAGLEA SVRYLASELG 190 200 210 220 230 240 SQNIRVNAIS AGPIRTLASS AVGGILDMIH HVEQVAPLRR TVTQLEVGNT AAFLASDLAS 250 GITGQVLYVD AGYEIMGM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection." Wei T.-F., Ramasubramanian T.S., Pu F., Golden J.W. J. Bacteriol. 175:4025-4035(1993) [PubMed: 8391534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L10036 Genomic DNA. Translation: AAD04184.1. Different initiation. BA000019 Genomic DNA. Translation: BAB76090.1. Different initiation.
PIR	AG2354.
RefSeq	NP_488431.1.
3D structure databases
HSSP	HSSP built from PDB template 1DFI based on UniProtKB P29132.
ModBase	Search...
Genome annotation databases
GeneID	1107992.
GenomeReviews	Gene locus all4391 in contig BA000019_GR.
KEGG	ana:all4391.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q05069.
Enzyme and pathway databases
BioCyc	NSP103690:ALL4391-MON.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit. PTHR19410:SF12. Enoyl-ACP_rdct. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
ProtoNet	Search...

Entry information

Entry name

FABI_ANASP

Accession

Primary (citable) accession number: Q05069

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	May 30, 2000
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)