Reviewed, UniProtKB/Swiss-Prot Q05065 (MUTB_STRCM)

Names and origin

Protein names

Recommended name:
    Methylmalonyl-CoA mutase large subunit
    EC=5.4.99.2
Alternative name(s):
    MCM-alpha

Gene names

Name:

mutB

Organism

Streptomyces cinnamonensis

Taxonomic identifier

1900 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	733 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthesis. It is unable to catalyze the conversion of isobutyryl-CoA into N-butyryl-CoA.
Catalytic activity	(R)-methylmalonyl-CoA = succinyl-CoA.
Cofactor	Adenosylcobalamin.
Pathway	Metabolic intermediate metabolism; propionyl-CoA degradation; succinyl-CoA from propionyl-CoA: step 3/3.
Subunit structure	Heterodimer of an alpha and a beta chain.
Sequence similarities	Belongs to the methylmalonyl-CoA mutase family. Contains 1 B12-binding domain.

Ontologies

Keywords
Ligand	Cobalamin Cobalt Metal-binding
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW methylmalonyl-CoA mutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 733	733	Methylmalonyl-CoA mutase large subunit		PRO_0000194275
Regions
	Domain	600 – 732	133	B12-binding
	Region	571 – 610	40	Methylmalonyl-CoA-binding site By similarity
Sites
	Metal binding	613	1	Cobalt (cobalamin axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05065-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: F02EEB54A154FA59	FASTA	733	79,455
	10 20 30 40 50 60 MRIPEFDDIE LGAGGGPSGS AEQWRAAVKE SVGKSESDLL WETPEGIAVK PLYTGADVEG 70 80 90 100 110 120 LDFLETYPGV APYLRGPYPT MYVNQPWTIR QYAGFSTAEE SNAFYRRNLA AGQKGLSVAF 130 140 150 160 170 180 DLPTHRGYDS DHPRVTGDVG MAGVAIDSIY DMRQLFDGIP LDKMTVSMTM NGAVLPVLAL 190 200 210 220 230 240 YIVAAEEQGV PPEKLAGTIQ NDILKEFMVR NTYIYPPKPS MRIISDIFAY TSQKMPRYNS 250 260 270 280 290 300 ISISGYHIQE AGATADLELA YTLADGVEYL RAGQEAGLDV DAFAPRLSFF WAIGMNFFME 310 320 330 340 350 360 VAKLRAARLL WAKLVKQFDP KNAKSLSLRT HSQTSGWSLT AQDVFNNVTR TCVEAMAATQ 370 380 390 400 410 420 GHTQSLHTNA LDEALALPTD FSARIARNTQ LLIQQESGTT RTIDPWGGSA YVEKLTYDLA 430 440 450 460 470 480 RRAWQHIEEV EAAGGMAQAI DAGIPKLRVE EAAARTQARI DSGRQPVIGV NKYRVDTDEQ 490 500 510 520 530 540 IDVLKVDNSS VRAQQIEKLR RLREERDDAA CQDALRALTA AAERGPGQGL EGNLLALAVD 550 560 570 580 590 600 AARAKATVGE ISDALESVYG RHAGQIRTIS GVYRTEAGQS PSVERTRALV DAFDEAEGRR 610 620 630 640 650 660 PRILVAKMGQ DGHDRGQKVI ASAFADLGFD VDVGPLFQTP AEVARQAVEA DVHIVGVSSL 670 680 690 700 710 720 AAGHLTLVPA LREELAAEGR DDIMIVVGGV IPPQDVEALH EAGATAVFPP GTVIPDAAHD 730 LVKRLAADLG HEL

References

[1]	"Cloning, sequencing, and expression of the gene encoding methylmalonyl-coenzyme A mutase from Streptomyces cinnamonensis." Birch A., Leiser A., Robinson J.A. J. Bacteriol. 175:3511-3519(1993) [PubMed: 8099072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3823.5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L10064 Unassigned DNA. Translation: AAA03041.1.
PIR	B40595.
3D structure databases
HSSP	HSSP built from PDB template 1REQ based on UniProtKB P11653.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.4.99.2. 1647.
Family and domain databases
InterPro	IPR006159. Acid_CoA_mut_C. IPR014348. Cbl-dep_enz_cat-sub. IPR006158. Cobalamin-bd. IPR006099. MMCoA_mutase_a/b_cat. IPR006098. MMCoA_mutase_a_cat. [Graphical view]
Gene3D	G3DSA:3.40.50.280. B12_bd. 1 hit. G3DSA:3.20.20.240. Cobalamin-dep_enz_cat. 1 hit.
Pfam	PF02310. B12-binding. 1 hit. PF01642. MM_CoA_mutase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00640. acid_CoA_mut_C. 1 hit. TIGR00641. acid_CoA_mut_N. 1 hit.
PROSITE	PS51332. B12_BINDING. 1 hit. PS00544. METMALONYL_COA_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MUTB_STRCM

Accession

Primary (citable) accession number: Q05065

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)