Reviewed, UniProtKB/Swiss-Prot Q05064 (MUTA_STRCM)

Names and origin

Protein names

Recommended name:
    Methylmalonyl-CoA mutase small subunit
    EC=5.4.99.2
Alternative name(s):
    MCM-beta

Gene names

Name:

mutA

Organism

Streptomyces cinnamonensis

Taxonomic identifier

1900 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthesis. It is unable to catalyze the conversion of isobutyryl-CoA into N-butyryl-CoA.
Catalytic activity	(R)-methylmalonyl-CoA = succinyl-CoA.
Cofactor	Adenosylcobalamin.
Pathway	Metabolic intermediate metabolism; propionyl-CoA degradation; succinyl-CoA from propionyl-CoA: step 3/3.
Subunit structure	Heterodimer of an alpha and a beta chain.
Sequence similarities	Belongs to the methylmalonyl-CoA mutase family.

Ontologies

Keywords
Ligand	Cobalamin Cobalt
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	lactate fermentation to propionate and acetate Inferred from electronic annotation. Source: InterPro
Molecular function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW methylmalonyl-CoA mutase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 616	616	Methylmalonyl-CoA mutase small subunit		PRO_0000194269

Sequences

	Sequence		Length	Mass (Da)
	Q05064-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 09CA24006E169AE6	FASTA	616	65,041
	10 20 30 40 50 60 MTVLPDDGLS LAAEFPDATH EQWHRLVEGV VRKSGKDVSG TAAEEALSTT LEDGLTTRPL 70 80 90 100 110 120 YTARDAAPDA GFPGFAPFVR GSVPEGNTPG GWDVRQRYAS ADPARTNEAV LTDLENGVTS 130 140 150 160 170 180 LWLTLGSAGL PVTGLERALD GVYLDLVPVA LDAGSEAATA ARELLRLYEA AGVADDAVRG 190 200 210 220 230 240 TLGADPLGHE ARTGEKSTSF AAVAELARLC GERYPGLRAL TVDALPYHEA GASAAQELGA 250 260 270 280 290 300 SLATGVEYLR ALHDKGLGVE KAFAQLEFRF AATADQFLTI AKLRAARRLW ARVAEVSGVP 310 320 330 340 350 360 AAGAQRQHAV TSPVMMTRRD PWVNMLRTTV ACLGAGVGGA DAVTVLPFDH ELGLPDAFAR 370 380 390 400 410 420 RIARNTSTIL LEESHLARVI DPAGGSWYVE RLTDELAHAA WDFFKEIERA DGQVAALRSG 430 440 450 460 470 480 LVGDRIAATW AERRKKLARR REPITGVSEF PLLTERPVER EPAPAAPPGG LPRVRRDEAY 490 500 510 520 530 540 EELRGRSDAH LEATGARPKV FIAALGPAAA HTARATFAAN LFMAGGVEPV HDPVSVDAET 550 560 570 580 590 600 AAEAFAASGA TVACLCSSDV LYAEQAEAVA RALKSAGALR VFLAGRGEFA DIDEYVFAGC 610 DAVAVLTSTL DRMGVA

References

[1]	"Cloning, sequencing, and expression of the gene encoding methylmalonyl-coenzyme A mutase from Streptomyces cinnamonensis." Birch A., Leiser A., Robinson J.A. J. Bacteriol. 175:3511-3519(1993) [PubMed: 8099072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3823.5.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L10064 Unassigned DNA. Translation: AAA03040.1.
PIR	A40595.
3D structure databases
HSSP	HSSP built from PDB template 2REQ based on UniProtKB P11652.
ModBase	Search...
Enzyme and pathway databases
BRENDA	5.4.99.2. 1647.
Family and domain databases
InterPro	IPR014348. Cbl-dep_enz_cat-sub. IPR006158. Cobalamin-bd. IPR006099. MMCoA_mutase_a/b_cat. IPR004608. MMCoA_mutase_b. [Graphical view]
Gene3D	G3DSA:3.40.50.280. B12_bd. 1 hit. G3DSA:3.20.20.240. Cobalamin-dep_enz_cat. 1 hit.
Pfam	PF01642. MM_CoA_mutase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00642. mmCoA_mut_beta. 1 hit.
PROSITE	PS00544. METMALONYL_COA_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MUTA_STRCM

Accession

Primary (citable) accession number: Q05064

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)