Reviewed, UniProtKB/Swiss-Prot Q05063 (LYOX_CHICK)

Names and origin

Protein names

Recommended name:
    Protein-lysine 6-oxidase
    EC=1.4.3.13
Alternative name(s):
    Lysyl oxidase

Gene names

Name:

LOX

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross linking of extracellular matrix proteins, it may have a direct role in tumor suppression.
Catalytic activity	Peptidyl-L-lysyl-peptide + O(2) + H(2)O = peptidyl-allysyl-peptide + NH(3) + H(2)O(2).
Cofactor	Copper By similarity. Contains 1 lysine tyrosylquinone By similarity.
Subcellular location	Secreted › extracellular space.
Developmental stage	Increases between day 8 and 16 of embryonic development, during aortic embryogenesis, in direct proportion to total protein synthesis.
Post-translational modification	The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.
Miscellaneous	The propeptide plays a role in directing the deposition of this enzyme to elastic fibers, via interaction with tropoelastin By similarity.
Sequence similarities	Belongs to the lysyl oxidase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein LTQ TPQ
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: InterPro protein-lysine 6-oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 171	150	By similarity		PRO_0000018526
	Chain	172 – 420	249	Protein-lysine 6-oxidase		PRO_0000018527
Regions
	Region	216 – 420	205	Lysyl-oxidase like
	Compositional bias	58 – 286	229	Arg/Pro-rich
Sites
	Metal binding	295	1	Copper Potential
	Metal binding	297	1	Copper Potential
	Metal binding	299	1	Copper Potential
Amino acid modifications
	Modified residue	358	1	2',4',5'-topaquinone By similarity
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Cross-link	323 ↔ 358		Lysine tyrosylquinone (Lys-Tyr) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05063-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: E0CBE81DD625F5C2	FASTA	420	48,153
	10 20 30 40 50 60 MRCAPPGLLL AQLHACIFWS GLWPAGCQSP PAAWRQRIQW ENNGQVYSLL SQGAQYQPPR 70 80 90 100 110 120 RRQGAEPASS PVLLLRGNGS VPRAAAAAAA RPQPEPQPQA QPQPRPRSSR RQPLGRRHWF 130 140 150 160 170 180 QAGYRAPSGS ARPAPRRRPR GRRSRRRERA ERRRAAAPSG LRPGREDVMV GDDPYSPYKY 190 200 210 220 230 240 TDDNPYYNYY DTYERPRQGS RYRPGYGTGY FQYGLPDLVP DPYYIQASTY VQRMSMYNLR 250 260 270 280 290 300 CAAEENCLAS SAYRADVRDY DNRVLLRFPQ RVKNQGTSDF LPSRPRYSWE WHSCHQHYHS 310 320 330 340 350 360 MDEFSHYDLL DASSHRKVAE GHKASFCLED TSCDYGYYRR YACTAHTQGL SPGCYDTYNA 370 380 390 400 410 420 DIDCQWIDIT DVKPGNYILK VSVNPSYLVP ESDYSNNIVR CDIRYTGHHA YASGCTISPY

References

[1]	"Characterization and developmental expression of chick aortic lysyl oxidase." Wu Y., Rich C.B., Lincecum J., Trackman P.C., Kagan H.M., Foster J.A. J. Biol. Chem. 267:24199-24206(1992) [PubMed: 1360009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M97881 mRNA. Translation: AAA48942.1.
PIR	A45166.
RefSeq	NP_990812.1.
UniGene	Gga.4303
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	396474.
KEGG	gga:396474.
Phylogenomic databases
HOGENOM	Q05063.
HOVERGEN	Q05063.
Family and domain databases
InterPro	IPR001695. Lysyl_oxidase. [Graphical view]
Pfam	PF01186. Lysyl_oxidase. 1 hit. [Graphical view]
PRINTS	PR00074. LYSYLOXIDASE.
PROSITE	PS00926. LYSYL_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYOX_CHICK

Accession

Primary (citable) accession number: Q05063

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	November 25, 2008
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)