Reviewed, UniProtKB/Swiss-Prot Q05053 (PAC1_PSESV)

Names and origin

Protein names

Recommended name:
    Acylase ACY 1
Cleaved into the following 2 chains:
    1- Recommended name:
            Acylase ACY 1 large subunit
    2- Recommended name:
            Acylase ACY 1 small subunit
Including the following 2 domains:
    1- Recommended name:
            Cephalosporin acylase
              EC=3.5.1.-
        Alternative name(s):
            GL-7ACA acylase
    2- Recommended name:
            Gamma-glutamyltranspeptidase
                Short name=GGT
              EC=2.3.2.2

Gene names

Name:

acyI

Organism

Pseudomonas sp. (strain V22)

Taxonomic identifier

33068 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Besides the cephalosporin acylase I activity which converts GL-7ACA into 7-ACA; this enzyme displays some gamma glutamyltranspeptidase activity.
Catalytic activity	7-beta-(4-carboxybutanamido)-cephalosporanic acid + H2O = 7-aminocephalosporanic acid + glutaric acid. (5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid.
Subunit structure	Dimer of two non-identical chains processed from the same precursor.
Sequence similarities	Belongs to the gamma-glutamyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Acyltransferase Hydrolase Transferase
PTM	Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – ?367	366	Acylase ACY 1 large subunit By similarity		PRO_0000011082
	Chain	368 – 558	191	Acylase ACY 1 small subunit By similarity		PRO_0000011083

Sequences

	Sequence		Length	Mass (Da)
	Q05053-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 22573EEACF69F5E4	FASTA	558	58,040
	10 20 30 40 50 60 MNAPVPVPRV ADFTCEKKPA TGSRGMVVTN HPLASAAGAQ ILLAGGNAID AAVASLFALT 70 80 90 100 110 120 VAEPMMVGIL GGGLSHIRLA DGRHVVIDNL STAPGKATAD MYECLSDEIG KQRDTRDREN 130 140 150 160 170 180 VVGAKAVAVP GALKGWCEAL ARFGTLPLAE VLQPAIGLAE RGFVVTPYLS NCITDNAADL 190 200 210 220 230 240 ARDPGLAAML LPGGQPLQPG MRLIQSDYAA SLKLIAAEGP EALYGGKLGR ALTDYMAANG 250 260 270 280 290 300 GLIDQADLSN YRIELREPIR GSYRGYEIIG PPPPSSSGVH IAQMLNILEG YDIGALGFGS 310 320 330 340 350 360 TDAVHLLAEA LKIAFADRAV ATADPAFVKV PVARLIDKAY ADERRALIAM EQAKSWTAGL 370 380 390 400 410 420 SGGESADTTH VTVADAMGNV VSATQTINGL FGACVQTPGT GMIANNYMYN FDPHPGRALS 430 440 450 460 470 480 IAPGKRVFTS MAPMMAVKEG RLAFALGLPG ALRIFPSALQ AIVNLIDHRM SLQEAVEAPR 490 500 510 520 530 540 VWTEGGVLEL EEAIPESVAQ ALIARGHKVV RSPRVAGGMN AIAFNPDGTL TGAACWRADG 550 TPVAISGGLA RAGARFTI

References

[1]	"Nucleotide sequence and expression in Escherichia coli of the cephalosporin acylase gene of a Pseudomonas strain." Ishiye M., Niwa M. Biochim. Biophys. Acta 1132:233-239(1992) [PubMed: 1358202] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-13 AND 368-381.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69020 Genomic DNA. Translation: CAA48785.1.
PIR	S27199.
3D structure databases
SMR	Q05053. Positions 16-375.
ModBase	Search...
Protein family/group databases
MEROPS	T03.001.
Family and domain databases
InterPro	IPR000101. GGT_peptidase. [Graphical view]
PANTHER	PTHR11686. GGT_peptidase. 1 hit.
Pfam	PF01019. G_glu_transpept. 1 hit. [Graphical view]
PRINTS	PR01210. GGTRANSPTASE.
TIGRFAMs	TIGR00066. g_glut_trans. 1 hit.
PROSITE	PS00462. G_GLU_TRANSPEPTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAC1_PSESV

Accession

Primary (citable) accession number: Q05053

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)