Q05052 (OST48_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Short name=DDOST 48 kDa subunit
Short name=Oligosaccharyl transferase 48 kDa subunit
EC=2.4.1.119

Gene names

Name:	DDOST
Synonyms:	OST48

Organism

Canis familiaris (Dog) (Canis lupus familiaris)

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.
Caution	It is uncertain whether Met-1 or Met-7 is the initiator.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complex Inferred from direct assay. Source: UniProtKB
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32	By similarity
	Chain	33 – 445	413	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000021955
Regions
	Topological domain	33 – 416	384	Lumenal Potential
	Transmembrane	417 – 436	20	Helical; Potential
	Topological domain	437 – 445	9	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	Q05052 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: C4F3384B4CE4CF66	FASTA	445	49,634
	10 20 30 40 50 60 MRRRRKMEAG AAARAWSLLW LLLPLLGPVC ASGPRTLVLL DNLNLRETHS LFFRSLKDRA 70 80 90 100 110 120 FELTFKTADD PSLSLIKYGE FLYDNLIIFS PSVEDFGGNI NVETISTFID GGGSVLVAAS 130 140 150 160 170 180 SDIGDPLREL GSECGIEFDE EKTAVIDHHN YDVSDLGQHT LIVADPENLL KAPTIVGRSS 190 200 210 220 230 240 LNPILFRGVG MVADPDNPLV LDILTGSSTS YSFFPDKPIT QYPHAVGKNT LLIAGLQARN 250 260 270 280 290 300 NARVVFSGSL DFFSDAFFNS AVQKAAPGSQ RYSQTGNYEL AVALSRWVFK EEGVLRVGPV 310 320 330 340 350 360 SHHRVGETAP PNAYTVTDLV EYSIVIEQLS NGRWVPFDGD DIQLEFVRID PFVRTFLKKK 370 380 390 400 410 420 GGKYSVQFKL PDVYGVFQFK VDYNRLGYTH LHSSTQVSVR PLQHTQYERF IPSAYPYYAS 430 440 AFSMMLGLFI FSTVFLHMKE KEKSD

References

[1]	"The 48-kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1." Silberstein S., Kelleher D.J., Gilmore R. J. Biol. Chem. 267:23658-23663(1992) [PubMed: 1429707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Cocker spaniel. Tissue: Kidney.
[2]	"Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits." Shibatani T., David L.L., McCormack A.L., Frueh K., Skach W.R. Biochemistry 44:5982-5992(2005) [PubMed: 15835887] [Abstract] Cited for: IDENTIFICATION IN THE OLIGOSACCHARYLTRANSFERASE COMPLEX.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M98392 mRNA. Translation: AAA30880.1.
PIR	A45139.
RefSeq	NP_001003321.1. NM_001003321.1.
UniGene	Cfa.44600.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q05052.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	404012.
KEGG	cfa:404012.
Organism-specific databases
CTD	1650.
Phylogenomic databases
eggNOG	maNOG09539.
GeneTree	ENSGT00390000017294.
HOVERGEN	HBG053378.
InParanoid	Q05052.
OrthoDB	EOG45HRXC.
Family and domain databases
InterPro	IPR005013. OligosaccharylTrfase_su_Wbp1. [Graphical view]
KO	K12670.
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OST48_CANFA

Accession

Primary (citable) accession number: Q05052

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program