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Protein

Cleavage stimulation factor subunit 1

Gene

CSTF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA processing

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72187 mRNA 3'-end processing
R-HSA-77595 Processing of Intronless Pre-mRNAs
SignaLinkiQ05048

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage stimulation factor subunit 1
Alternative name(s):
CF-1 50 kDa subunit
Cleavage stimulation factor 50 kDa subunit
Short name:
CSTF 50 kDa subunit
Short name:
CstF-50
Gene namesi
Name:CSTF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000101138.11
HGNCiHGNC:2483 CSTF1
MIMi600369 gene
neXtProtiNX_Q05048

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1477
OpenTargetsiENSG00000101138
PharmGKBiPA26985

Polymorphism and mutation databases

BioMutaiCSTF1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509441 – 431Cleavage stimulation factor subunit 1Add BLAST431

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

EPDiQ05048
PaxDbiQ05048
PeptideAtlasiQ05048
PRIDEiQ05048

2D gel databases

REPRODUCTION-2DPAGEiIPI00011528

PTM databases

iPTMnetiQ05048
PhosphoSitePlusiQ05048

Expressioni

Gene expression databases

BgeeiENSG00000101138
CleanExiHS_CSTF1
ExpressionAtlasiQ05048 baseline and differential
GenevisibleiQ05048 HS

Organism-specific databases

HPAiCAB019270
HPA047275
HPA065000

Interactioni

Subunit structurei

Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). Interacts directly with CSTF3. Interacts with BARD1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi107859, 49 interactors
CORUMiQ05048
IntActiQ05048, 12 interactors
STRINGi9606.ENSP00000217109

Structurei

Secondary structure

1431
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi96 – 98Combined sources3
Beta strandi100 – 106Combined sources7
Beta strandi111 – 116Combined sources6
Beta strandi120 – 127Combined sources8
Beta strandi132 – 136Combined sources5
Helixi137 – 141Combined sources5
Beta strandi164 – 169Combined sources6
Beta strandi176 – 181Combined sources6
Beta strandi183 – 192Combined sources10
Beta strandi195 – 201Combined sources7
Beta strandi211 – 215Combined sources5
Beta strandi220 – 225Combined sources6
Beta strandi229 – 240Combined sources12
Beta strandi242 – 245Combined sources4
Turni246 – 248Combined sources3
Beta strandi251 – 253Combined sources3
Helixi257 – 259Combined sources3
Beta strandi265 – 270Combined sources6
Beta strandi274 – 281Combined sources8
Beta strandi286 – 290Combined sources5
Turni291 – 293Combined sources3
Beta strandi296 – 300Combined sources5
Turni301 – 306Combined sources6
Beta strandi309 – 314Combined sources6
Beta strandi318 – 325Combined sources8
Beta strandi330 – 334Combined sources5
Turni335 – 338Combined sources4
Beta strandi339 – 344Combined sources6
Beta strandi350 – 352Combined sources3
Beta strandi365 – 372Combined sources8
Turni373 – 376Combined sources4
Beta strandi377 – 382Combined sources6
Turni383 – 385Combined sources3
Beta strandi400 – 405Combined sources6
Beta strandi407 – 416Combined sources10
Beta strandi419 – 425Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6B3XX-ray2.30A80-431[»]
ProteinModelPortaliQ05048
SMRiQ05048
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati106 – 145WD 1Add BLAST40
Repeati171 – 210WD 2Add BLAST40
Repeati215 – 254WD 3Add BLAST40
Repeati260 – 301WD 4Add BLAST42
Repeati303 – 343WD 5Add BLAST41
Repeati395 – 430WD 6Add BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 35HydrophobicAdd BLAST22

Domaini

The WD6 domain is required for interaction with BARD1. WD domains are responsible for interaction with CSTF3.
N-terminus mediates homodimerization.

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0640 Eukaryota
ENOG410XPWJ LUCA
GeneTreeiENSGT00910000144253
HOGENOMiHOG000234077
HOVERGENiHBG051144
InParanoidiQ05048
KOiK14406
OMAiITHSPNA
OrthoDBiEOG091G07AW
PhylomeDBiQ05048
TreeFamiTF314234

Family and domain databases

Gene3Di1.20.960.50, 1 hit
2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR032028 CSTF1_dimer
IPR038184 CSTF1_dimer_sf
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF16699 CSTF1_dimer, 1 hit
PF00400 WD40, 5 hits
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 1 hit
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q05048-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL
60 70 80 90 100
LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE
110 120 130 140 150
TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV
160 170 180 190 200
MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF
210 220 230 240 250
DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ
260 270 280 290 300
CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF
310 320 330 340 350
EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS
360 370 380 390 400
GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV
410 420 430
RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT D
Length:431
Mass (Da):48,358
Last modified:February 1, 1994 - v1
Checksum:i88A5BE53022AD9E3
GO

Sequence cautioni

The sequence CAC12718 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02547 mRNA Translation: AAA35691.1
BT007138 mRNA Translation: AAP35802.1
AK312774 mRNA Translation: BAG35638.1
AL121914 Genomic DNA Translation: CAC12718.2 Sequence problems.
AL121914 Genomic DNA Translation: CAI19328.1
CH471077 Genomic DNA Translation: EAW75548.1
CH471077 Genomic DNA Translation: EAW75549.1
BC001011 mRNA Translation: AAH01011.1
BC007425 mRNA Translation: AAH07425.1
CCDSiCCDS13452.1
PIRiA45142
RefSeqiNP_001028693.1, NM_001033521.1
NP_001028694.1, NM_001033522.1
NP_001315.1, NM_001324.2
XP_011526902.1, XM_011528600.1
UniGeneiHs.172865

Genome annotation databases

EnsembliENST00000217109; ENSP00000217109; ENSG00000101138
GeneIDi1477
KEGGihsa:1477
UCSCiuc002xxm.2 human

Similar proteinsi

Entry informationi

Entry nameiCSTF1_HUMAN
AccessioniPrimary (citable) accession number: Q05048
Secondary accession number(s): Q5QPD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: April 25, 2018
This is version 181 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health