Reviewed, UniProtKB/Swiss-Prot Q05048 (CSTF1_HUMAN)

Names and origin

Protein names

Recommended name:
    Cleavage stimulation factor 50 kDa subunit
      Short name=CSTF 50 kDa subunit
Alternative name(s):
    CF-1 50 kDa subunit
    CstF-50

Gene names

Name:

CSTF1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.
Subunit structure	Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). Interacts directly with CSTF3. Interacts with BARD1. Ref.5 Ref.6
Subcellular location	Nucleus.
Domain	The WD6 domain is required for interaction with BARD1. WD domains are responsible for interaction with CSTF3. N-terminus mediates homodimerization.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Contains 6 WD repeats.
Sequence caution	The sequence CAC12718.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	RNA splicing Inferred from Experiment. Source: Reactome mRNA cleavage Ref.1 Traceable author statement. Source: ProtInc mRNA polyadenylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleus Ref.1 Traceable author statement. Source: ProtInc
Molecular function	RNA binding Traceable author statement. Source: ProtInc protein binding Ref.5 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BARD1	Q99728	4	EBI-1789619,EBI-473181
PCNA	P12004	1	EBI-1789619,EBI-358311
POLR1A	O95602	1	EBI-1789619,EBI-359472

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Cleavage stimulation factor 50 kDa subunit		PRO_0000050944
Regions
	Repeat	106 – 145	40	WD 1
	Repeat	171 – 210	40	WD 2
	Repeat	215 – 254	40	WD 3
	Repeat	260 – 301	42	WD 4
	Repeat	303 – 343	41	WD 5
	Repeat	395 – 430	36	WD 6
	Region	14 – 35	22	Hydrophobic
Amino acid modifications
	Modified residue	367	1	Phosphotyrosine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q05048-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 88A5BE53022AD9E3	FASTA	431	48,358
	10 20 30 40 50 60 MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN 70 80 90 100 110 120 DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG 130 140 150 160 170 180 QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA 190 200 210 220 230 240 FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT 250 260 270 280 290 300 LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF 310 320 330 340 350 360 EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV 370 380 390 400 410 420 FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR 430 ARFWYRRSTT D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human polyadenylation factor is a G protein beta-subunit homologue." Takagaki Y., Manley J.L. J. Biol. Chem. 267:23471-23474(1992) [PubMed: 1358884] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 101-119 AND 155-170.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[5]	"Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50." Kleiman F.E., Manley J.L. Science 285:1576-1579(1999) [PubMed: 10477523] [Abstract] Cited for: INTERACTION WITH BARD1.
[6]	"Complex protein interactions within the human polyadenylation machinery identify a novel component." Takagaki Y., Manley J.L. Mol. Cell. Biol. 20:1515-1525(2000) [PubMed: 10669729] [Abstract] Cited for: SUBUNIT.
[7]	"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-367, MASS SPECTROMETRY.
[8]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L02547 mRNA. Translation: AAA35691.1. BT007138 mRNA. Translation: AAP35802.1. AL121914 Genomic DNA. Translation: CAC12718.2. Sequence problems. BC001011 mRNA. Translation: AAH01011.1. BC007425 mRNA. Translation: AAH07425.1.
IPI	IPI00011528.
PIR	A45142.
RefSeq	NP_001028693.1. NP_001028694.1. NP_001315.1.
UniGene	Hs.172865
3D structure databases
SMR	Q05048. Positions 44-426.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q05048. 3 interactions.
STRING	Q05048.
PTM databases
PhosphoSite	Q05048.
2-D gel databases
REPRODUCTION-2DPAGE	IPI00011528.
Proteomic databases
PeptideAtlas	Q05048.
PRIDE	Q05048.
Genome annotation databases
Ensembl	ENST00000217109; ENSP00000217109; ENSG00000101138; Homo sapiens. [Genome view] ENST00000425890; ENSP00000398984; ENSG00000101138; Homo sapiens. [Genome view] ENST00000451236; ENSP00000415266; ENSG00000101138; Homo sapiens. [Genome view]
GeneID	1477.
KEGG	hsa:1477.
UCSC	uc002xxl.1. human.
Organism-specific databases
CTD	1477.
GeneCards	GC20P054401.
H-InvDB	HIX0015931.
HGNC	HGNC:2483. CSTF1.
HPA	CAB019270.
MIM	600369. gene.
PharmGKB	PA26985.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG595084.
HOVERGEN	Q05048.
InParanoid	Q05048.
OMA	YINIANG.
OrthoDB	EOG979HSF.
PhylomeDB	Q05048.
Enzyme and pathway databases
Pathway_Interaction_DB	bard1pathway. BARD1 signaling events.
Reactome	REACT_125. Processing of Capped Intron-Containing Pre-mRNA. REACT_1788. Transcription. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	Q05048.
Bgee	Q05048.
CleanEx	HS_CSTF1.
Genevestigator	Q05048.
GermOnline	ENSG00000101138. Homo sapiens.
Family and domain databases
InterPro	IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00400. WD40. 6 hits. [Graphical view]
SMART	SM00320. WD40. 6 hits. [Graphical view]
PROSITE	PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 4 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	6065.
SOURCE	Search...

Entry information

Entry name

CSTF1_HUMAN

Accession

Primary (citable) accession number: Q05048

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	February 9, 2010
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.