Q05048 (CSTF1_HUMAN) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 116.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Cleavage stimulation factor subunit 1 Alternative name(s): CF-1 50 kDa subunit Cleavage stimulation factor 50 kDa subunit Short name=CSTF 50 kDa subunit Short name=CstF-50 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 431 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA. |
| Subunit structure | Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). Interacts directly with CSTF3. Interacts with BARD1. Ref.5 Ref.6 |
| Subcellular location | |
| Domain | The WD6 domain is required for interaction with BARD1. WD domains are responsible for interaction with CSTF3. N-terminus mediates homodimerization. |
| Post-translational modification | The N-terminus is blocked. |
| Sequence similarities | Contains 6 WD repeats. |
| Sequence caution | The sequence CAC12718.2 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Biological process | mRNA processing |
| Cellular component | Nucleus |
| Domain | Repeat WD repeat |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | mRNA cleavage Traceable author statement. Source: ProtInc mRNA polyadenylationTraceable author statement. Source: ProtInc nuclear mRNA splicing, via spliceosomeTraceable author statement. Source: Reactome termination of RNA polymerase II transcriptionTraceable author statement. Source: Reactome |
| Cellular component | nucleoplasm Traceable author statement. Source: Reactome |
| Molecular function | RNA binding Traceable author statement. Source: ProtInc protein bindingInferred from physical interaction Ref.5. Source: UniProtKB |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| BARD1 | Q99728 | 4 | EBI-1789619,EBI-473181 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 431 | 431 | Cleavage stimulation factor subunit 1 | PRO_0000050944 | |||||
Regions | |||||||||
| Repeat | 106 – 145 | 40 | WD 1 | ||||||
| Repeat | 171 – 210 | 40 | WD 2 | ||||||
| Repeat | 215 – 254 | 40 | WD 3 | ||||||
| Repeat | 260 – 301 | 42 | WD 4 | ||||||
| Repeat | 303 – 343 | 41 | WD 5 | ||||||
| Repeat | 395 – 430 | 36 | WD 6 | ||||||
| Region | 14 – 35 | 22 | Hydrophobic | ||||||
Amino acid modifications | |||||||||
| Modified residue | 367 | 1 | Phosphotyrosine Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A human polyadenylation factor is a G protein beta-subunit homologue." Takagaki Y., Manley J.L. J. Biol. Chem. 267:23471-23474(1992) [PubMed: 1358884] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 101-119 AND 155-170. |
| [2] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [3] | "The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. Rogers J.Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle. |
| [5] | "Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50." Kleiman F.E., Manley J.L. Science 285:1576-1579(1999) [PubMed: 10477523] [Abstract] Cited for: INTERACTION WITH BARD1. |
| [6] | "Complex protein interactions within the human polyadenylation machinery identify a novel component." Takagaki Y., Manley J.L. Mol. Cell. Biol. 20:1515-1525(2000) [PubMed: 10669729] [Abstract] Cited for: SUBUNIT. |
| [7] | "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J. Nat. Biotechnol. 23:94-101(2005) [PubMed: 15592455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-367, MASS SPECTROMETRY. |
| [8] | "Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | L02547 mRNA. Translation: AAA35691.1. BT007138 mRNA. Translation: AAP35802.1. AL121914 Genomic DNA. Translation: CAC12718.2. Sequence problems. BC001011 mRNA. Translation: AAH01011.1. BC007425 mRNA. Translation: AAH07425.1. |
| IPI | IPI00011528. |
| PIR | A45142. |
| RefSeq | NP_001028693.1. NM_001033521.1. NP_001028694.1. NM_001033522.1. NP_001315.1. NM_001324.2. |
| UniGene | Hs.172865. |
3D structure databases | |
| ProteinModelPortal | Q05048. |
| SMR | Q05048. Positions 5-60, 91-428. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q05048. 4 interactions. |
| STRING | Q05048. |
PTM databases | |
| PhosphoSite | Q05048. |
Polymorphism databases | |
| DMDM | 461848. |
2D gel databases | |
| REPRODUCTION-2DPAGE | IPI00011528. |
Proteomic databases | |
| PeptideAtlas | Q05048. |
| PRIDE | Q05048. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENST00000217109; ENSP00000217109; ENSG00000101138. ENST00000425890; ENSP00000398984; ENSG00000101138. ENST00000451236; ENSP00000415266; ENSG00000101138. |
| GeneID | 1477. |
| KEGG | hsa:1477. |
| UCSC | uc002xxl.1. human. |
Organism-specific databases | |
| CTD | 1477. |
| GeneCards | GC20P054967. |
| H-InvDB | HIX0015931. |
| HGNC | HGNC:2483. CSTF1. |
| HPA | CAB019270. |
| MIM | 600369. gene. |
| neXtProt | NX_Q05048. |
| PharmGKB | PA26985. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | HBG595084. |
| HOVERGEN | HBG051144. |
| InParanoid | Q05048. |
| OMA | LYDHTDE. |
| OrthoDB | EOG4868CD. |
| PhylomeDB | Q05048. |
Enzyme and pathway databases | |
| Pathway_Interaction_DB | bard1pathway. BARD1 signaling events. |
| Reactome | REACT_1675. mRNA Processing. REACT_1788. Transcription. REACT_71. Gene Expression. REACT_78. Post-Elongation Processing of the Transcript. |
Gene expression databases | |
| ArrayExpress | Q05048. |
| Bgee | Q05048. |
| CleanEx | HS_CSTF1. |
| Genevestigator | Q05048. |
| GermOnline | ENSG00000101138. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view] |
| Gene3D | G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit. |
| KO | K14406. |
| Pfam | PF00400. WD40. 6 hits. [Graphical view] |
| SMART | SM00320. WD40. 6 hits. [Graphical view] |
| SUPFAM | SSF50978. WD40_like. 1 hit. |
| PROSITE | PS00678. WD_REPEATS_1. 1 hit. PS50082. WD_REPEATS_2. 4 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 6065. |
| SOURCE | Search... |
Entry information
| Entry name | CSTF1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q05048 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 20 Human chromosome 20: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with