ID   C72A1_CATRO             Reviewed;         524 AA.
AC   Q05047;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   16-JUN-2009, entry version 71.
DE   RecName: Full=Secologanin synthase;
DE            Short=SLS;
DE            EC=1.3.3.9;
DE   AltName: Full=Cytochrome P450 72A1;
DE   AltName: Full=CYPLXXII;
GN   Name=CYP72A1; Synonyms=CYP72, P450CR3;
OS   Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Catharanthus.
OX   NCBI_TaxID=4058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. CP3A;
RX   PubMed=16653087;
RA   Vetter H.-P., Mangold U., Schroeder G., Marner F.-J.,
RA   Werck-Reichhart D., Schroeder J.;
RT   "Molecular analysis and heterologous expression of an inducible
RT   cytochrome P-450 protein from periwinkle (Catharanthus roseus L.).";
RL   Plant Physiol. 100:998-1007(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 469-524.
RC   STRAIN=cv. G. Don;
RX   MEDLINE=93283641; PubMed=8507838; DOI=10.1007/BF00014944;
RA   Meijer A.H., Souer E., Verpoorte R., Hoge J.H.C.;
RT   "Isolation of cytochrome P-450 cDNA clones from the higher plant
RT   Catharanthus roseus by a PCR strategy.";
RL   Plant Mol. Biol. 22:379-383(1993).
RN   [3]
RP   FUNCTION.
RC   STRAIN=cv. CP3A;
RX   MEDLINE=20575722; PubMed=11135113;
RX   DOI=10.1046/j.1365-313x.2000.00922.x;
RA   Irmler S., Schroeder G., St Pierre B., Crouch N.P., Hotze M.,
RA   Schmidt J., Strack D., Matern U., Schroeder J.;
RT   "Indole alkaloid biosynthesis in Catharanthus roseus: new enzyme
RT   activities and identification of cytochrome P450 CYP72A1 as
RT   secologanin synthase.";
RL   Plant J. 24:797-804(2000).
CC   -!- FUNCTION: Converts loganin into secologanin.
CC   -!- CATALYTIC ACTIVITY: Loganin + NADPH + O(2) = secologanin + NADP(+)
CC       + 2 H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Alkaloid biosynthesis; secologanin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane (Probable). Note=Tonoplast
CC       (Probable).
CC   -!- TISSUE SPECIFICITY: Upper and lower leaf epidermis.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; L10081; AAA33106.1; -; mRNA.
DR   EMBL; X69775; CAA49430.1; -; mRNA.
DR   PIR; S35168; S35168.
DR   PIR; T09944; T09944.
DR   HSSP; P14779; 1JPZ.
DR   BRENDA; 1.3.3.9; 20471.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0050616; F:secologanin synthase activity; IEA:EC.
DR   GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Alkaloid metabolism; Heme; Iron; Membrane; Metal-binding;
KW   Monooxygenase; NADP; Oxidoreductase; Transmembrane; Vacuole.
FT   CHAIN         1    524       Secologanin synthase.
FT                                /FTId=PRO_0000052124.
FT   TRANSMEM     12     32       Potential.
FT   METAL       470    470       Iron (heme axial ligand) (By similarity).
FT   VARIANT     190    190       I -> L.
FT   VARIANT     194    194       Q -> E.
FT   VARIANT     223    223       E -> D.
FT   VARIANT     312    312       K -> R.
FT   VARIANT     318    318       S -> T.
FT   VARIANT     403    403       V -> I.
FT   VARIANT     405    405       K -> E.
FT   VARIANT     411    411       S -> P.
SQ   SEQUENCE   524 AA;  60558 MW;  EF5D864E43C751E8 CRC64;
     MEMDMDTIRK AIAATIFALV MAWAWRVLDW AWFTPKRIEK RLRQQGFRGN PYRFLVGDVK
     ESGKMHQEAL SKPMEFNNDI VPRLMPHINH TINTYGRNSF TWMGRIPRIH VMEPELIKEV
     LTHSSKYQKN FDVHNPLVKF LLTGVGSFEG AKWSKHRRII SPAFTLEKLK SMLPAFAICY
     HDMLTKWEKI AEKQGSHEVD IFPTFDVLTS DVISKVAFGS TYEEGGKIFR LLKELMDLTI
     DCMRDVYIPG WSYLPTKRNK RMKEINKEIT DMLRFIINKR MKALKAGEPG EDDLLGVLLE
     SNIQEIQKQG NKKDGGMSIN DVIEECKLFY FAGQETTGVL LTWTTILLSK HPEWQERARE
     EVLQAFGKNK PEFERLNHLK YVSMILYEVL RLYPPVIDLT KIVHKDTKLG SYTIPAGTQV
     MLPTVMLHRE KSIWGEDAME FNPMRFVDGV ANATKNNVTY LPFSWGPRVC LGQNFALLQA
     KLGLAMILQR FKFDVAPSYV HAPFTILTVQ PQFGSHVIYK KLES
//