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Protein

Secologanin synthase

Gene

CYP72A1

Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Converts loganin into secologanin.1 Publication

Catalytic activityi

Loganin + NADPH + O2 = secologanin + NADP+ + 2 H2O.

Cofactori

hemeBy similarity

Pathwayi: secologanin biosynthesis

This protein is involved in the pathway secologanin biosynthesis, which is part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway secologanin biosynthesis and in Alkaloid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi470 – 4701Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Alkaloid metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.3.3.9. 1211.
UniPathwayiUPA00328.

Names & Taxonomyi

Protein namesi
Recommended name:
Secologanin synthase (EC:1.3.3.9)
Short name:
SLS
Alternative name(s):
CYPLXXII
Cytochrome P450 72A1
Gene namesi
Name:CYP72A1
Synonyms:CYP72, P450CR3
OrganismiCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Taxonomic identifieri4058 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei12 – 3221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 524524Secologanin synthasePRO_0000052124Add
BLAST

Expressioni

Tissue specificityi

Upper and lower leaf epidermis.

Structurei

3D structure databases

ProteinModelPortaliQ05047.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK13400.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05047-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMDMDTIRK AIAATIFALV MAWAWRVLDW AWFTPKRIEK RLRQQGFRGN
60 70 80 90 100
PYRFLVGDVK ESGKMHQEAL SKPMEFNNDI VPRLMPHINH TINTYGRNSF
110 120 130 140 150
TWMGRIPRIH VMEPELIKEV LTHSSKYQKN FDVHNPLVKF LLTGVGSFEG
160 170 180 190 200
AKWSKHRRII SPAFTLEKLK SMLPAFAICY HDMLTKWEKI AEKQGSHEVD
210 220 230 240 250
IFPTFDVLTS DVISKVAFGS TYEEGGKIFR LLKELMDLTI DCMRDVYIPG
260 270 280 290 300
WSYLPTKRNK RMKEINKEIT DMLRFIINKR MKALKAGEPG EDDLLGVLLE
310 320 330 340 350
SNIQEIQKQG NKKDGGMSIN DVIEECKLFY FAGQETTGVL LTWTTILLSK
360 370 380 390 400
HPEWQERARE EVLQAFGKNK PEFERLNHLK YVSMILYEVL RLYPPVIDLT
410 420 430 440 450
KIVHKDTKLG SYTIPAGTQV MLPTVMLHRE KSIWGEDAME FNPMRFVDGV
460 470 480 490 500
ANATKNNVTY LPFSWGPRVC LGQNFALLQA KLGLAMILQR FKFDVAPSYV
510 520
HAPFTILTVQ PQFGSHVIYK KLES
Length:524
Mass (Da):60,558
Last modified:February 1, 1994 - v1
Checksum:iEF5D864E43C751E8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti190 – 1901I → L.
Natural varianti194 – 1941Q → E.
Natural varianti223 – 2231E → D.
Natural varianti312 – 3121K → R.
Natural varianti318 – 3181S → T.
Natural varianti403 – 4031V → I.
Natural varianti405 – 4051K → E.
Natural varianti411 – 4111S → P.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10081 mRNA. Translation: AAA33106.1.
X69775 mRNA. Translation: CAA49430.1.
PIRiS35168.
T09944.

Genome annotation databases

KEGGiag:AAA33106.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10081 mRNA. Translation: AAA33106.1.
X69775 mRNA. Translation: CAA49430.1.
PIRiS35168.
T09944.

3D structure databases

ProteinModelPortaliQ05047.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAA33106.

Phylogenomic databases

KOiK13400.

Enzyme and pathway databases

UniPathwayiUPA00328.
BRENDAi1.3.3.9. 1211.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular analysis and heterologous expression of an inducible cytochrome P-450 protein from periwinkle (Catharanthus roseus L.)."
    Vetter H.-P., Mangold U., Schroeder G., Marner F.-J., Werck-Reichhart D., Schroeder J.
    Plant Physiol. 100:998-1007(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. CP3A.
  2. "Isolation of cytochrome P-450 cDNA clones from the higher plant Catharanthus roseus by a PCR strategy."
    Meijer A.H., Souer E., Verpoorte R., Hoge J.H.C.
    Plant Mol. Biol. 22:379-383(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 469-524.
    Strain: cv. G. Don.
  3. "Indole alkaloid biosynthesis in Catharanthus roseus: new enzyme activities and identification of cytochrome P450 CYP72A1 as secologanin synthase."
    Irmler S., Schroeder G., St Pierre B., Crouch N.P., Hotze M., Schmidt J., Strack D., Matern U., Schroeder J.
    Plant J. 24:797-804(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: cv. CP3A.

Entry informationi

Entry nameiC72A1_CATRO
AccessioniPrimary (citable) accession number: Q05047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: November 11, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.