Reviewed, UniProtKB/Swiss-Prot Q05047 (C72A1_CATRO)

Names and origin

Protein names

Recommended name:
    Secologanin synthase
      Short name=SLS
    EC=1.3.3.9
Alternative name(s):
    Cytochrome P450 72A1
    CYPLXXII

Gene names

Name:	CYP72A1
Synonyms:	CYP72, P450CR3

Organism

Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)

Taxonomic identifier

4058 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Catharanthus

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts loganin into secologanin. Ref.3
Catalytic activity	Loganin + NADPH + O2 = secologanin + NADP+ + 2 H2O.
Cofactor	Heme group By similarity.
Pathway	Alkaloid biosynthesis; secologanin biosynthesis.
Subcellular location	Vacuole membrane Probable. Note: Tonoplast Probable.
Tissue specificity	Upper and lower leaf epidermis.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Cellular component	Membrane Vacuole
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW vacuolar membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW secologanin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Secologanin synthase		PRO_0000052124
Regions
	Transmembrane	12 – 32	21	Potential
Sites
	Metal binding	470	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	190	1	I → L
	Natural variant	194	1	Q → E
	Natural variant	223	1	E → D
	Natural variant	312	1	K → R
	Natural variant	318	1	S → T
	Natural variant	403	1	V → I
	Natural variant	405	1	K → E
	Natural variant	411	1	S → P

Sequences

	Sequence		Length	Mass (Da)
	Q05047-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: EF5D864E43C751E8	FASTA	524	60,558
	10 20 30 40 50 60 MEMDMDTIRK AIAATIFALV MAWAWRVLDW AWFTPKRIEK RLRQQGFRGN PYRFLVGDVK 70 80 90 100 110 120 ESGKMHQEAL SKPMEFNNDI VPRLMPHINH TINTYGRNSF TWMGRIPRIH VMEPELIKEV 130 140 150 160 170 180 LTHSSKYQKN FDVHNPLVKF LLTGVGSFEG AKWSKHRRII SPAFTLEKLK SMLPAFAICY 190 200 210 220 230 240 HDMLTKWEKI AEKQGSHEVD IFPTFDVLTS DVISKVAFGS TYEEGGKIFR LLKELMDLTI 250 260 270 280 290 300 DCMRDVYIPG WSYLPTKRNK RMKEINKEIT DMLRFIINKR MKALKAGEPG EDDLLGVLLE 310 320 330 340 350 360 SNIQEIQKQG NKKDGGMSIN DVIEECKLFY FAGQETTGVL LTWTTILLSK HPEWQERARE 370 380 390 400 410 420 EVLQAFGKNK PEFERLNHLK YVSMILYEVL RLYPPVIDLT KIVHKDTKLG SYTIPAGTQV 430 440 450 460 470 480 MLPTVMLHRE KSIWGEDAME FNPMRFVDGV ANATKNNVTY LPFSWGPRVC LGQNFALLQA 490 500 510 520 KLGLAMILQR FKFDVAPSYV HAPFTILTVQ PQFGSHVIYK KLES

References

[1]	"Molecular analysis and heterologous expression of an inducible cytochrome P-450 protein from periwinkle (Catharanthus roseus L.)." Vetter H.-P., Mangold U., Schroeder G., Marner F.-J., Werck-Reichhart D., Schroeder J. Plant Physiol. 100:998-1007(1992) [PubMed: 16653087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. CP3A.
[2]	"Isolation of cytochrome P-450 cDNA clones from the higher plant Catharanthus roseus by a PCR strategy." Meijer A.H., Souer E., Verpoorte R., Hoge J.H.C. Plant Mol. Biol. 22:379-383(1993) [PubMed: 8507838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 469-524. Strain: cv. G. Don.
[3]	"Indole alkaloid biosynthesis in Catharanthus roseus: new enzyme activities and identification of cytochrome P450 CYP72A1 as secologanin synthase." Irmler S., Schroeder G., St Pierre B., Crouch N.P., Hotze M., Schmidt J., Strack D., Matern U., Schroeder J. Plant J. 24:797-804(2000) [PubMed: 11135113] [Abstract] Cited for: FUNCTION. Strain: cv. CP3A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L10081 mRNA. Translation: AAA33106.1. X69775 mRNA. Translation: CAA49430.1.
PIR	S35168. T09944.
3D structure databases
SMR	Q05047. Positions 93-520.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.3.9. 20471.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C72A1_CATRO

Accession

Primary (citable) accession number: Q05047

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	January 19, 2010
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)