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Protein

Mannan endo-1,6-alpha-mannosidase DFG5

Gene

DFG5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for normal synthesis of the cell wall.1 Publication

Catalytic activityi

Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans.

GO - Molecular functioni

GO - Biological processi

  • budding cell bud growth Source: SGD
  • carbohydrate catabolic process Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • fungal-type cell wall biogenesis Source: SGD
  • pseudohyphal growth Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-32919-MONOMER.

Protein family/group databases

CAZyiGH76. Glycoside Hydrolase Family 76.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,6-alpha-mannosidase DFG5 (EC:3.2.1.101)
Alternative name(s):
Endo-alpha-1->6-D-mannanase DFG5
Gene namesi
Name:DFG5
Ordered Locus Names:YMR238W
ORF Names:YM9959.20
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR238W.
SGDiS000004851. DFG5.

Subcellular locationi

  • Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication

  • Note: GPI-anchored plasma membrane protein (GPI-PMP).

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence analysisAdd
BLAST
Chaini27 – 437411Mannan endo-1,6-alpha-mannosidase DFG5PRO_0000012127Add
BLAST
Propeptidei438 – 45821Removed in mature formSequence analysisPRO_0000012128Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence analysis
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence analysis
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence analysis
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence analysis
Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence analysis
Glycosylationi245 – 2451N-linked (GlcNAc...)Sequence analysis
Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence analysis
Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence analysis
Glycosylationi417 – 4171N-linked (GlcNAc...)Sequence analysis
Lipidationi437 – 4371GPI-anchor amidated glycineSequence analysis

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ05031.
PeptideAtlasiQ05031.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AGP1P253761EBI-27512,EBI-2357
CAN1P048171EBI-27512,EBI-3993
CHO1P084561EBI-27512,EBI-14055
FLC2P397191EBI-27512,EBI-20703
GAP1P191451EBI-27512,EBI-7314
ORM2Q061441EBI-27512,EBI-34916
OST1P415431EBI-27512,EBI-12651
PMP2P409751EBI-27512,EBI-2043041
PMP3P872841EBI-27512,EBI-13555
SAC1P323681EBI-27512,EBI-16210
SPF1P399861EBI-27512,EBI-3128
YHR146WP388451EBI-27512,EBI-24770

Protein-protein interaction databases

BioGridi35416. 59 interactions.
DIPiDIP-2739N.
IntActiQ05031. 6 interactions.
MINTiMINT-500290.

Structurei

3D structure databases

ProteinModelPortaliQ05031.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 76 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000002859.
HOGENOMiHOG000178818.
InParanoidiQ05031.
KOiK08257.
OMAiFGGMLEN.
OrthoDBiEOG72RN80.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005198. Glyco_hydro_76.
IPR014480. Mannan-1_6-alpha_mannosidase.
[Graphical view]
PfamiPF03663. Glyco_hydro_76. 1 hit.
[Graphical view]
PIRSFiPIRSF016302. Man_a_manosd. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05031-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVNISAKMI LSICFTFLSF FKATHAMDLD TTSKTSICDA TALIQGGMLD
60 70 80 90 100
YYEGTRYGGT VGMFQSPYYW WHAGEAFGGM LENWFLCEND TYQELLYDAL
110 120 130 140 150
LAQTGSNYDY IPSNQTMVEG NDDQGIWGIT VMGAVERNFT DPGDGKPGWL
160 170 180 190 200
AMVQAVFNTM YSRWDSEHCG GGLRWQIFTW NSGYNYKNTV SNACLFQIAA
210 220 230 240 250
RLGRYTGNTT YLEVAEQVFD WLVDVGYVVL NDTANVFDGA EIDTNCTDIT
260 270 280 290 300
KIEWTYNHGI VLGGLAYMYN ATNGTGEWET SLTKILNGAK SYFFKDSIMY
310 320 330 340 350
ESACQDYGTC NTDQRTFKSI FSRMLGLTSV MAPFTRDTID DLIKTSAEAA
360 370 380 390 400
AKSCNGGTDG HTCGLNWQKQ TNDGYYGLGE QMSALEVIQN LLIHDRPAPY
410 420 430 440 450
KEDNGGTSKG DANAGMNSST TNVLQNNLNI KKGDRAGAAI ITAVILSVLT

GGAVWMLF
Length:458
Mass (Da):50,541
Last modified:November 1, 1997 - v1
Checksum:i908D7E672333EC03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49939 Genomic DNA. Translation: CAA90209.1.
BK006946 Genomic DNA. Translation: DAA10138.1.
PIRiS57605.
RefSeqiNP_013965.1. NM_001182745.1.

Genome annotation databases

EnsemblFungiiYMR238W; YMR238W; YMR238W.
GeneIDi855278.
KEGGisce:YMR238W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49939 Genomic DNA. Translation: CAA90209.1.
BK006946 Genomic DNA. Translation: DAA10138.1.
PIRiS57605.
RefSeqiNP_013965.1. NM_001182745.1.

3D structure databases

ProteinModelPortaliQ05031.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35416. 59 interactions.
DIPiDIP-2739N.
IntActiQ05031. 6 interactions.
MINTiMINT-500290.

Protein family/group databases

CAZyiGH76. Glycoside Hydrolase Family 76.

Proteomic databases

MaxQBiQ05031.
PeptideAtlasiQ05031.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR238W; YMR238W; YMR238W.
GeneIDi855278.
KEGGisce:YMR238W.

Organism-specific databases

EuPathDBiFungiDB:YMR238W.
SGDiS000004851. DFG5.

Phylogenomic databases

GeneTreeiENSGT00390000002859.
HOGENOMiHOG000178818.
InParanoidiQ05031.
KOiK08257.
OMAiFGGMLEN.
OrthoDBiEOG72RN80.

Enzyme and pathway databases

BioCyciYEAST:G3O-32919-MONOMER.

Miscellaneous databases

NextBioi978906.
PROiQ05031.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR005198. Glyco_hydro_76.
IPR014480. Mannan-1_6-alpha_mannosidase.
[Graphical view]
PfamiPF03663. Glyco_hydro_76. 1 hit.
[Graphical view]
PIRSFiPIRSF016302. Man_a_manosd. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Two homologous genes, DCW1 (YKL046c) and DFG5, are essential for cell growth and encode glycosylphosphatidylinositol (GPI)-anchored membrane proteins required for cell wall biogenesis in Saccharomyces cerevisiae."
    Kitagaki H., Wu H., Shimoi H., Ito K.
    Mol. Microbiol. 46:1011-1022(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes."
    Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.
    Mol. Syst. Biol. 5:308-308(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDFG5_YEAST
AccessioniPrimary (citable) accession number: Q05031
Secondary accession number(s): D6W064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2950 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.