Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mannan endo-1,6-alpha-mannosidase DFG5

Gene

DFG5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for normal synthesis of the cell wall.1 Publication

Miscellaneous

Present with 2950 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans.

GO - Molecular functioni

GO - Biological processi

  • budding cell bud growth Source: SGD
  • carbohydrate catabolic process Source: InterPro
  • cell wall organization Source: UniProtKB-KW
  • fungal-type cell wall biogenesis Source: SGD
  • pseudohyphal growth Source: SGD

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:G3O-32919-MONOMER

Protein family/group databases

CAZyiGH76 Glycoside Hydrolase Family 76

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,6-alpha-mannosidase DFG5 (EC:3.2.1.101)
Alternative name(s):
Endo-alpha-1->6-D-mannanase DFG5
Gene namesi
Name:DFG5
Ordered Locus Names:YMR238W
ORF Names:YM9959.20
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR238W
SGDiS000004851 DFG5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000001212727 – 437Mannan endo-1,6-alpha-mannosidase DFG5Add BLAST411
PropeptideiPRO_0000012128438 – 458Removed in mature formSequence analysisAdd BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi89N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi138N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi208N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi231N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi245N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi270N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi273N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi417N-linked (GlcNAc...) asparagineSequence analysis1
Lipidationi437GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ05031
PaxDbiQ05031
PRIDEiQ05031

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ORM2Q061443EBI-27512,EBI-34916

Protein-protein interaction databases

BioGridi35416, 119 interactors
DIPiDIP-2739N
IntActiQ05031, 14 interactors
MINTiQ05031
STRINGi4932.YMR238W

Structurei

3D structure databases

ProteinModelPortaliQ05031
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 76 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00390000002859
HOGENOMiHOG000178818
InParanoidiQ05031
KOiK08257
OMAiTTDMLSF
OrthoDBiEOG092C22S6

Family and domain databases

InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR005198 Glyco_hydro_76
IPR014480 Mannan-1_6-alpha_mannosidase
PANTHERiPTHR12145 PTHR12145, 1 hit
PfamiView protein in Pfam
PF03663 Glyco_hydro_76, 1 hit
PIRSFiPIRSF016302 Man_a_manosd, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05031-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVNISAKMI LSICFTFLSF FKATHAMDLD TTSKTSICDA TALIQGGMLD
60 70 80 90 100
YYEGTRYGGT VGMFQSPYYW WHAGEAFGGM LENWFLCEND TYQELLYDAL
110 120 130 140 150
LAQTGSNYDY IPSNQTMVEG NDDQGIWGIT VMGAVERNFT DPGDGKPGWL
160 170 180 190 200
AMVQAVFNTM YSRWDSEHCG GGLRWQIFTW NSGYNYKNTV SNACLFQIAA
210 220 230 240 250
RLGRYTGNTT YLEVAEQVFD WLVDVGYVVL NDTANVFDGA EIDTNCTDIT
260 270 280 290 300
KIEWTYNHGI VLGGLAYMYN ATNGTGEWET SLTKILNGAK SYFFKDSIMY
310 320 330 340 350
ESACQDYGTC NTDQRTFKSI FSRMLGLTSV MAPFTRDTID DLIKTSAEAA
360 370 380 390 400
AKSCNGGTDG HTCGLNWQKQ TNDGYYGLGE QMSALEVIQN LLIHDRPAPY
410 420 430 440 450
KEDNGGTSKG DANAGMNSST TNVLQNNLNI KKGDRAGAAI ITAVILSVLT

GGAVWMLF
Length:458
Mass (Da):50,541
Last modified:November 1, 1997 - v1
Checksum:i908D7E672333EC03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49939 Genomic DNA Translation: CAA90209.1
BK006946 Genomic DNA Translation: DAA10138.1
PIRiS57605
RefSeqiNP_013965.1, NM_001182745.1

Genome annotation databases

EnsemblFungiiYMR238W; YMR238W; YMR238W
GeneIDi855278
KEGGisce:YMR238W

Similar proteinsi

Entry informationi

Entry nameiDFG5_YEAST
AccessioniPrimary (citable) accession number: Q05031
Secondary accession number(s): D6W064
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health