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Protein

Transcription initiation factor TFIID subunit 9

Gene

TAF9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID and the transcription regulatory histone acetylation (HAT) complex SAGA and SLIK. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3, and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.10 Publications

Miscellaneous

Present with 7000 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • chromatin binding Source: SGD
  • identical protein binding Source: IntAct
  • protein-containing complex scaffold activity Source: SGD
  • protein heterodimerization activity Source: InterPro
  • transcription factor binding Source: GO_Central
  • transcription regulatory region DNA binding Source: GO_Central

GO - Biological processi

  • chromatin organization Source: SGD
  • histone acetylation Source: SGD
  • histone H3 acetylation Source: GO_Central
  • positive regulation of transcription by RNA polymerase II Source: GO_Central
  • RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  • transcription by RNA polymerase II Source: SGD

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32917-MONOMER
ReactomeiR-SCE-5689880 Ub-specific processing proteases
R-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6807505 RNA polymerase II transcribes snRNA genes
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 9
Alternative name(s):
TAFII-17
TAFII20
TBP-associated factor 17 kDa
TBP-associated factor 9
Gene namesi
Name:TAF9
Synonyms:TAF17
Ordered Locus Names:YMR236W
ORF Names:YM9959.18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR236W
SGDiS000004849 TAF9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001188961 – 157Transcription initiation factor TFIID subunit 9Add BLAST157

Proteomic databases

MaxQBiQ05027
PaxDbiQ05027
PRIDEiQ05027

PTM databases

iPTMnetiQ05027

Interactioni

Subunit structurei

In TFIID, TAF9 heterodimerizes with TAF6, forming ultimately an octamer consisting of a TAF6/TAF9 heterotetramer core flanked by TAF4/TAF12 dimers on either side, similar to the histone H2A/H2B/H3/H4 octamer. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Component of the 1.8 MDa SAGA complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SGF73, SPT20/ADA5, SPT8, TAF12, TAF6, HFI1/ADA1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10, TAF9, SGF11 and SUS1. TAF5, TAF6, TAF9, TAF19, TAF12 and ADA1 seem to be present in 2 copies. SAGA is built of 5 distinct domains with specialized functions. Domain I (containing TRA1) probably represents the activator interaction surface. Domain II (containing TAF5 and TAF6, and probably TAF9 and TAF10), domain III (containing GCN5, TAF10, SPT7, TAF5 and ADA1, and probably ADA2, ADA3 and TAF12), and domain IV (containing HFI1/ADA1 and TAF6, and probably TAF9) are believed to play primarily an architectural role. Domain III also harbors the HAT activity. Domain V (containing SPT3 and SPT20, and probably SPT8) represents the TBP-interacting module, which may be associated transiently with SAGA. Component of the SLIK complex, which consists of at least TRA1, CHD1, SPT7, TAF5, ADA3, SPT20, RTG2, TAF12, TAF6, HFI1, UBP8, GCN5, ADA2, SPT3, SGF29, TAF10 AND TAF9.5 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein-containing complex scaffold activity Source: SGD
  • protein heterodimerization activity Source: InterPro
  • transcription factor binding Source: GO_Central

Protein-protein interaction databases

BioGridi35414, 454 interactors
ComplexPortaliCPX-1642 General transcription factor complex TFIID
CPX-656 SAGA complex
CPX-675 SLIK (SAGA-like) complex
DIPiDIP-925N
IntActiQ05027, 153 interactors
MINTiQ05027
STRINGi4932.YMR236W

Structurei

3D structure databases

ProteinModelPortaliQ05027
SMRiQ05027
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 97Histone-foldAdd BLAST68

Sequence similaritiesi

Belongs to the TAF9 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000001626
HOGENOMiHOG000204913
InParanoidiQ05027
KOiK03133
OMAiTNYQFKP
OrthoDBiEOG092C55QK

Family and domain databases

CDDicd07979 TAF9, 1 hit
Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR009072 Histone-fold
IPR003162 TFIID-31
PfamiView protein in Pfam
PF02291 TFIID-31kDa, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit

Sequencei

Sequence statusi: Complete.

Q05027-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGGGKNVLN KNSVGSVSEV GPDSTQEETP RDVRLLHLLL ASQSIHQYED
60 70 80 90 100
QVPLQLMDFA HRYTQGVLKD ALVYNDYAGS GNSAGSGLGV EDIRLAIAAR
110 120 130 140 150
TQYQFKPTAP KELMLQLAAE RNKKALPQVM GTWGVRLPPE KYCLTAKEWD

LEDPKSM
Length:157
Mass (Da):17,315
Last modified:November 1, 1997 - v1
Checksum:iD1C97EDDCA468EC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49939 Genomic DNA Translation: CAA90207.1
BK006946 Genomic DNA Translation: DAA10136.1
PIRiS57603
RefSeqiNP_013963.1, NM_001182743.1

Genome annotation databases

EnsemblFungiiYMR236W; YMR236W; YMR236W
GeneIDi855276
KEGGisce:YMR236W

Similar proteinsi

Entry informationi

Entry nameiTAF9_YEAST
AccessioniPrimary (citable) accession number: Q05027
Secondary accession number(s): D6W062
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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