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Q05021

- TAF7_YEAST

UniProt

Q05021 - TAF7_YEAST

Protein

Transcription initiation factor TFIID subunit 7

Gene

TAF7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. TAF7 is responsible for the recruitment of BDF1 to TATA element containing promoters.4 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
    3. transcription from RNA polymerase II promoter Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32908-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor TFIID subunit 7
    Alternative name(s):
    TAFII-67
    TBP-associated factor 67 kDa
    TBP-associated factor 7
    Gene namesi
    Name:TAF7
    Synonyms:TAF67
    Ordered Locus Names:YMR227C
    ORF Names:YM9959.09C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR227c.
    SGDiS000004840. TAF7.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. transcription factor TFIID complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 590590Transcription initiation factor TFIID subunit 7PRO_0000118885Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei99 – 991Phosphotyrosine1 Publication
    Modified residuei101 – 1011Phosphoserine1 Publication
    Modified residuei104 – 1041Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ05021.
    PaxDbiQ05021.
    PeptideAtlasiQ05021.

    Expressioni

    Gene expression databases

    GenevestigatoriQ05021.

    Interactioni

    Subunit structurei

    TAF7 interacts with BDF1. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BDF1P358175EBI-27490,EBI-3493

    Protein-protein interaction databases

    BioGridi35405. 87 interactions.
    DIPiDIP-6818N.
    IntActiQ05021. 13 interactions.
    MINTiMINT-636201.
    STRINGi4932.YMR227C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05021.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili427 – 549123Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi7 – 9084Lys-richAdd
    BLAST
    Compositional biasi200 – 2034Poly-Glu
    Compositional biasi304 – 571268Asp/Glu-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TAF7 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5414.
    HOGENOMiHOG000142022.
    KOiK03132.
    OMAiPPMRWAR.
    OrthoDBiEOG70PC83.

    Family and domain databases

    InterProiIPR006751. TAFII55_prot_cons_reg.
    [Graphical view]
    PfamiPF04658. TAFII55_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q05021-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVIRIKKPR GPGEKDQPLE GEPKLKRIRI KTKVTDEDIK PKPKLKINLK    50
    KKKESADGKE KKNSLKLKLN LKKNEEPVKK IHKAPKLRLK PIRIPGEAYD 100
    SEASDIEDDP LIESGVILRI LPDIQLEFVK NSLESGDYSG ISIKWKNERH 150
    AVVTINDVMY GAILVDLPTV IEVNKSVDRK NLLKTFDVSQ MLLCIRPIQE 200
    EEEVYALEAP DTEDLVVKHF EGIEDEIWEN KETFLKGYNG APLSDMEAKH 250
    LKEIALKGYD YKHGISPPLY NVRNRRFRRK MDPNEIDYVE KVVDMLLKQD 300
    KQAEEVSYDL VDKSELQARQ ERVSSWENFK EEPGEPLSRP ALKKEEIHTI 350
    ASAVGKQGAE EEGEEGMEEE EEEDLDLGAA FESEEEGSGA EGDKEQQQEE 400
    VGDEVDQDTG GEDDDDDDDG DIEAAGGESE SDDEKDENRQ HTELLADELN 450
    ELETTLAHTK HKLSKATNPL LKSRFIDSIK KLEKEAELKR KQLQQTEDSV 500
    QKQHQHRSDA ETANNVEEEE EEEEEEEEED EVDEDEEDDE ENDEDEDNVH 550
    EREHIQENKV VRELDEAPAE ETLDQNDLDM MMLFGAEGDE 590
    Length:590
    Mass (Da):67,555
    Last modified:November 1, 1997 - v1
    Checksum:iC014E7419B0B1C61
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49939 Genomic DNA. Translation: CAA90198.1.
    BK006946 Genomic DNA. Translation: DAA10126.1.
    PIRiS57594.
    RefSeqiNP_013954.1. NM_001182734.1.

    Genome annotation databases

    EnsemblFungiiYMR227C; YMR227C; YMR227C.
    GeneIDi855267.
    KEGGisce:YMR227C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49939 Genomic DNA. Translation: CAA90198.1 .
    BK006946 Genomic DNA. Translation: DAA10126.1 .
    PIRi S57594.
    RefSeqi NP_013954.1. NM_001182734.1.

    3D structure databases

    ProteinModelPortali Q05021.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35405. 87 interactions.
    DIPi DIP-6818N.
    IntActi Q05021. 13 interactions.
    MINTi MINT-636201.
    STRINGi 4932.YMR227C.

    Proteomic databases

    MaxQBi Q05021.
    PaxDbi Q05021.
    PeptideAtlasi Q05021.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR227C ; YMR227C ; YMR227C .
    GeneIDi 855267.
    KEGGi sce:YMR227C.

    Organism-specific databases

    CYGDi YMR227c.
    SGDi S000004840. TAF7.

    Phylogenomic databases

    eggNOGi COG5414.
    HOGENOMi HOG000142022.
    KOi K03132.
    OMAi PPMRWAR.
    OrthoDBi EOG70PC83.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32908-MONOMER.

    Miscellaneous databases

    NextBioi 978873.
    PROi Q05021.

    Gene expression databases

    Genevestigatori Q05021.

    Family and domain databases

    InterProi IPR006751. TAFII55_prot_cons_reg.
    [Graphical view ]
    Pfami PF04658. TAFII55_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
      Sanders S.L., Weil P.A.
      J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    4. "Bromodomain factor 1 corresponds to a missing piece of yeast TFIID."
      Matangkasombut O., Buratowski R.M., Swilling N.W., Buratowski S.
      Genes Dev. 14:951-962(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH BDF1.
    5. "Molecular characterization of Saccharomyces cerevisiae TFIID."
      Sanders S.L., Garbett K.A., Weil P.A.
      Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TFIID STOICHIOMETRY.
    6. "Multi-protein complexes in eukaryotic gene transcription."
      Martinez E.
      Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-99 AND SER-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101 AND SER-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTAF7_YEAST
    AccessioniPrimary (citable) accession number: Q05021
    Secondary accession number(s): D6W052
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1100 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3