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Q05021

- TAF7_YEAST

UniProt

Q05021 - TAF7_YEAST

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Protein

Transcription initiation factor TFIID subunit 7

Gene

TAF7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. TAF7 is responsible for the recruitment of BDF1 to TATA element containing promoters.4 Publications

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  3. transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32908-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 7
Alternative name(s):
TAFII-67
TBP-associated factor 67 kDa
TBP-associated factor 7
Gene namesi
Name:TAF7
Synonyms:TAF67
Ordered Locus Names:YMR227C
ORF Names:YM9959.09C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR227c.
SGDiS000004840. TAF7.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. transcription factor TFIID complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 590590Transcription initiation factor TFIID subunit 7PRO_0000118885Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei99 – 991Phosphotyrosine1 Publication
Modified residuei101 – 1011Phosphoserine1 Publication
Modified residuei104 – 1041Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ05021.
PaxDbiQ05021.
PeptideAtlasiQ05021.

Expressioni

Gene expression databases

GenevestigatoriQ05021.

Interactioni

Subunit structurei

TAF7 interacts with BDF1. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BDF1P358175EBI-27490,EBI-3493

Protein-protein interaction databases

BioGridi35405. 87 interactions.
DIPiDIP-6818N.
IntActiQ05021. 13 interactions.
MINTiMINT-636201.
STRINGi4932.YMR227C.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OY2X-ray2.90B/D/F86-320[»]
ProteinModelPortaliQ05021.
SMRiQ05021. Positions 87-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili427 – 549123Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 9084Lys-richAdd
BLAST
Compositional biasi200 – 2034Poly-Glu
Compositional biasi304 – 571268Asp/Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the TAF7 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG5414.
HOGENOMiHOG000142022.
InParanoidiQ05021.
KOiK03132.
OMAiPPMRWAR.
OrthoDBiEOG70PC83.

Family and domain databases

InterProiIPR006751. TAFII55_prot_cons_reg.
[Graphical view]
PfamiPF04658. TAFII55_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05021 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVIRIKKPR GPGEKDQPLE GEPKLKRIRI KTKVTDEDIK PKPKLKINLK
60 70 80 90 100
KKKESADGKE KKNSLKLKLN LKKNEEPVKK IHKAPKLRLK PIRIPGEAYD
110 120 130 140 150
SEASDIEDDP LIESGVILRI LPDIQLEFVK NSLESGDYSG ISIKWKNERH
160 170 180 190 200
AVVTINDVMY GAILVDLPTV IEVNKSVDRK NLLKTFDVSQ MLLCIRPIQE
210 220 230 240 250
EEEVYALEAP DTEDLVVKHF EGIEDEIWEN KETFLKGYNG APLSDMEAKH
260 270 280 290 300
LKEIALKGYD YKHGISPPLY NVRNRRFRRK MDPNEIDYVE KVVDMLLKQD
310 320 330 340 350
KQAEEVSYDL VDKSELQARQ ERVSSWENFK EEPGEPLSRP ALKKEEIHTI
360 370 380 390 400
ASAVGKQGAE EEGEEGMEEE EEEDLDLGAA FESEEEGSGA EGDKEQQQEE
410 420 430 440 450
VGDEVDQDTG GEDDDDDDDG DIEAAGGESE SDDEKDENRQ HTELLADELN
460 470 480 490 500
ELETTLAHTK HKLSKATNPL LKSRFIDSIK KLEKEAELKR KQLQQTEDSV
510 520 530 540 550
QKQHQHRSDA ETANNVEEEE EEEEEEEEED EVDEDEEDDE ENDEDEDNVH
560 570 580 590
EREHIQENKV VRELDEAPAE ETLDQNDLDM MMLFGAEGDE
Length:590
Mass (Da):67,555
Last modified:November 1, 1997 - v1
Checksum:iC014E7419B0B1C61
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49939 Genomic DNA. Translation: CAA90198.1.
BK006946 Genomic DNA. Translation: DAA10126.1.
PIRiS57594.
RefSeqiNP_013954.1. NM_001182734.1.

Genome annotation databases

EnsemblFungiiYMR227C; YMR227C; YMR227C.
GeneIDi855267.
KEGGisce:YMR227C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49939 Genomic DNA. Translation: CAA90198.1 .
BK006946 Genomic DNA. Translation: DAA10126.1 .
PIRi S57594.
RefSeqi NP_013954.1. NM_001182734.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4OY2 X-ray 2.90 B/D/F 86-320 [» ]
ProteinModelPortali Q05021.
SMRi Q05021. Positions 87-315.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35405. 87 interactions.
DIPi DIP-6818N.
IntActi Q05021. 13 interactions.
MINTi MINT-636201.
STRINGi 4932.YMR227C.

Proteomic databases

MaxQBi Q05021.
PaxDbi Q05021.
PeptideAtlasi Q05021.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR227C ; YMR227C ; YMR227C .
GeneIDi 855267.
KEGGi sce:YMR227C.

Organism-specific databases

CYGDi YMR227c.
SGDi S000004840. TAF7.

Phylogenomic databases

eggNOGi COG5414.
HOGENOMi HOG000142022.
InParanoidi Q05021.
KOi K03132.
OMAi PPMRWAR.
OrthoDBi EOG70PC83.

Enzyme and pathway databases

BioCyci YEAST:G3O-32908-MONOMER.

Miscellaneous databases

NextBioi 978873.
PROi Q05021.

Gene expression databases

Genevestigatori Q05021.

Family and domain databases

InterProi IPR006751. TAFII55_prot_cons_reg.
[Graphical view ]
Pfami PF04658. TAFII55_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
    Sanders S.L., Weil P.A.
    J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  4. "Bromodomain factor 1 corresponds to a missing piece of yeast TFIID."
    Matangkasombut O., Buratowski R.M., Swilling N.W., Buratowski S.
    Genes Dev. 14:951-962(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BDF1.
  5. "Molecular characterization of Saccharomyces cerevisiae TFIID."
    Sanders S.L., Garbett K.A., Weil P.A.
    Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TFIID STOICHIOMETRY.
  6. "Multi-protein complexes in eukaryotic gene transcription."
    Martinez E.
    Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-99 AND SER-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101 AND SER-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTAF7_YEAST
AccessioniPrimary (citable) accession number: Q05021
Secondary accession number(s): D6W052
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3