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Q05021 (TAF7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription initiation factor TFIID subunit 7
Alternative name(s):
TAFII-67
TBP-associated factor 67 kDa
TBP-associated factor 7
Gene names
Name:TAF7
Synonyms:TAF67
Ordered Locus Names:YMR227C
ORF Names:YM9959.09C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length590 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. TAF7 is responsible for the recruitment of BDF1 to TATA element containing promoters. Ref.3 Ref.4 Ref.5 Ref.6

Subunit structure

TAF7 interacts with BDF1. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Ref.3 Ref.4

Subcellular location

Nucleus Ref.7.

Miscellaneous

Present with 1100 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TAF7 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BDF1P358175EBI-27490,EBI-3493

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 590590Transcription initiation factor TFIID subunit 7
PRO_0000118885

Regions

Coiled coil427 – 549123 Potential
Compositional bias7 – 9084Lys-rich
Compositional bias200 – 2034Poly-Glu
Compositional bias304 – 571268Asp/Glu-rich

Amino acid modifications

Modified residue991Phosphotyrosine Ref.10
Modified residue1011Phosphoserine Ref.11
Modified residue1041Phosphoserine Ref.10 Ref.11

Sequences

Sequence LengthMass (Da)Tools
Q05021 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: C014E7419B0B1C61

FASTA59067,555
        10         20         30         40         50         60 
MAVIRIKKPR GPGEKDQPLE GEPKLKRIRI KTKVTDEDIK PKPKLKINLK KKKESADGKE 

        70         80         90        100        110        120 
KKNSLKLKLN LKKNEEPVKK IHKAPKLRLK PIRIPGEAYD SEASDIEDDP LIESGVILRI 

       130        140        150        160        170        180 
LPDIQLEFVK NSLESGDYSG ISIKWKNERH AVVTINDVMY GAILVDLPTV IEVNKSVDRK 

       190        200        210        220        230        240 
NLLKTFDVSQ MLLCIRPIQE EEEVYALEAP DTEDLVVKHF EGIEDEIWEN KETFLKGYNG 

       250        260        270        280        290        300 
APLSDMEAKH LKEIALKGYD YKHGISPPLY NVRNRRFRRK MDPNEIDYVE KVVDMLLKQD 

       310        320        330        340        350        360 
KQAEEVSYDL VDKSELQARQ ERVSSWENFK EEPGEPLSRP ALKKEEIHTI ASAVGKQGAE 

       370        380        390        400        410        420 
EEGEEGMEEE EEEDLDLGAA FESEEEGSGA EGDKEQQQEE VGDEVDQDTG GEDDDDDDDG 

       430        440        450        460        470        480 
DIEAAGGESE SDDEKDENRQ HTELLADELN ELETTLAHTK HKLSKATNPL LKSRFIDSIK 

       490        500        510        520        530        540 
KLEKEAELKR KQLQQTEDSV QKQHQHRSDA ETANNVEEEE EEEEEEEEED EVDEDEEDDE 

       550        560        570        580        590 
ENDEDEDNVH EREHIQENKV VRELDEAPAE ETLDQNDLDM MMLFGAEGDE 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
Sanders S.L., Weil P.A.
J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[4]"Bromodomain factor 1 corresponds to a missing piece of yeast TFIID."
Matangkasombut O., Buratowski R.M., Swilling N.W., Buratowski S.
Genes Dev. 14:951-962(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH BDF1.
[5]"Molecular characterization of Saccharomyces cerevisiae TFIID."
Sanders S.L., Garbett K.A., Weil P.A.
Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TFIID STOICHIOMETRY.
[6]"Multi-protein complexes in eukaryotic gene transcription."
Martinez E.
Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Mapping histone fold TAFs within yeast TFIID."
Leurent C., Sanders S.L., Ruhlmann C., Mallouh V., Weil P.A., Kirschner D.B., Tora L., Schultz P.
EMBO J. 21:3424-3433(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-99 AND SER-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-101 AND SER-104, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49939 Genomic DNA. Translation: CAA90198.1.
BK006946 Genomic DNA. Translation: DAA10126.1.
PIRS57594.
RefSeqNP_013954.1. NM_001182734.1.

3D structure databases

ProteinModelPortalQ05021.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35405. 87 interactions.
DIPDIP-6818N.
IntActQ05021. 13 interactions.
MINTMINT-636201.
STRING4932.YMR227C.

Proteomic databases

MaxQBQ05021.
PaxDbQ05021.
PeptideAtlasQ05021.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR227C; YMR227C; YMR227C.
GeneID855267.
KEGGsce:YMR227C.

Organism-specific databases

CYGDYMR227c.
SGDS000004840. TAF7.

Phylogenomic databases

eggNOGCOG5414.
HOGENOMHOG000142022.
KOK03132.
OMAPPMRWAR.
OrthoDBEOG70PC83.

Enzyme and pathway databases

BioCycYEAST:G3O-32908-MONOMER.

Gene expression databases

GenevestigatorQ05021.

Family and domain databases

InterProIPR006751. TAFII55_prot_cons_reg.
[Graphical view]
PfamPF04658. TAFII55_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978873.
PROQ05021.

Entry information

Entry nameTAF7_YEAST
AccessionPrimary (citable) accession number: Q05021
Secondary accession number(s): D6W052
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families