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Q05017

- PLB1_RABIT

UniProt

Q05017 - PLB1_RABIT

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Protein
Phospholipase B1, membrane-associated
Gene
PLB1
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Can convert phosphatidylcholine to fatty acids and glycerophosphocholine. Could be involved in uptake of dietary lipids, possibly including long chain retinyl esters.1 Publication

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei400 – 4001Nucleophile By similarity
Binding sitei474 – 4741Substrate; via amide nitrogen By similarity
Binding sitei513 – 5131Substrate By similarity
Active sitei1347 – 13471 By similarity
Active sitei1350 – 13501 By similarity

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC
  2. phospholipase A2 activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Alternative name(s):
Phospholipase AdRab-B
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLB1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Apical cell membrane; Single-pass type I membrane protein By similarity
Note: Present in the intestinal brush border membranes.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 14151396Extracellular Reviewed prediction
Add
BLAST
Transmembranei1416 – 143621Helical; Reviewed prediction
Add
BLAST
Topological domaini1437 – 145822Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 14581439Phospholipase B1, membrane-associated
PRO_0000017854Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi493 – 4931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi529 – 5291N-linked (GlcNAc...) Reviewed prediction
Glycosylationi590 – 5901N-linked (GlcNAc...) Reviewed prediction
Glycosylationi690 – 6901N-linked (GlcNAc...) Reviewed prediction
Glycosylationi783 – 7831N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum By similarity.

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Intestine.1 Publication

Developmental stagei

Expressed in the intestine of adult but not baby rabbits.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013064.

Structurei

3D structure databases

ProteinModelPortaliQ05017.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati39 – 3473091
Add
BLAST
Repeati362 – 7073462
Add
BLAST
Repeati708 – 10543473
Add
BLAST
Repeati1064 – 14033404
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni39 – 140313654 X 308-326 AA approximate repeats
Add
BLAST

Domaini

Repeat 2 contains the catalytic domain By similarity.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG311176.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.

Family and domain databases

Gene3Di3.40.50.1110. 6 hits.
InterProiIPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05017-1 [UniParc]FASTAAdd to Basket

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MALWPSVFLL GLLPLLGRGA DQIQTSSGKN TLEGQLWPES LKTFPFPCDP     50
KTLAESVPSE SVHSLRPSDI KFVAAIGNVE TAPDSGADDL EEQDGTEKRP 100
EQACMGVVTV LSDIIGRFSP SALMPLCPET RLVPRGGAED LWMQATELVR 150
SMRENPQLDF EHDWKLINVF FSNTSQCFPC PSAQQKGLVL GGMDKLTRTL 200
DYLQQEVPKA FVNLVDLSEL AAFSRWRQGA QLSPAAEPCR CLRETSQLTK 250
VLTQWSYLEA WDSLLASSKY NTQESFAVVF QPFFYESSLS ALLAEPPLQD 300
PTTLALSLWN RMMEPIGRKE EPFSEKERKP LRCPTQESPY LFTYRNSGQL 350
TRVSQPQGKL EVREGTEIRC PDKDPSDSVP TSVHRLKPAD IKVIGAMGDS 400
LTAGNGAGSQ PGNILDVLTQ YRGLSWSVGG DQNISTVTTL ANILREFNPS 450
LQGFSVGTGR ETTSQAFFNQ AVAGARADGL IPQAQRLVAL MKNDTRINFQ 500
EDWKIITVFI GGNDLCDFCN DPVRYSPQNF TDNIGTALDI LHAEIPRAFV 550
NLVKVLEISK LRELYQETKV SCPRMILRSL CPCVLKFDDN STEIASLIET 600
IKEYQERTQQ LIDSGRYDTR DDFTVVLQPF FEKVNMPKTQ DGLPDNSFFA 650
PDCFHFSSKA HAHAASALWN NMLEPVGQKT THNDFEGAVN ITCPNQVWPF 700
LSTYKNSVQG FGTWLPCRDR SPSASPPTSV HALRPADIQV VAALGDSLTA 750
GIGIGSKPND LSDGTTQYRG LSYSSGGDGS LDNVTTLPNI LRQFNSNLMG 800
FAVGTGDASG TNAFFNQAVP GAKARDLMSQ VQTLVQRMKD DHRVNFQEDW 850
KVITVQIGAS DLCDYCTDSN LYSAANFYDH LRDALDALHR EVPRALVNLV 900
DFMNPSVTRQ VFLGNPDKCP VQQASALCNC VLSPRENSYE LARLEALAQA 950
YQSSLRELVE SGRYDTREDF SVVLQPFFHS IQLPVLQDGR LDTSFFAPDC 1000
VHPNQKFHSQ LSRALWRNML EPLGGKTDAL DLTAAITLTC PTQNEPFLRT 1050
FRNSDYTYPS RPAVENWGSD FLCTAWNASR GVPNSVHELQ PGDIKVVAAL 1100
GDSLTLAMGA RPSNSSDPPM FWRGLSWSIG GDGALETHTT LPNILKKFNP 1150
SILGFSTGTL EGTMGLNVAV QGARAQDMPA QARDLVERMR NSPEIDLEKD 1200
WKLVTLFVGG NDLCHFCENP EGSSEGEYVQ HIQQALDVLY EELPRTFVNV 1250
VEVMELAGLH QDQGGRCATL LAAQSHCTCF KYSQSSVEMQ ELKKVNWNLQ 1300
SGLSRLSYSH QYVQREDFAV VVQPFFQNTL VPLNGRGDTD LTFFSDDCFH 1350
FSERGHAEMA IALWNNMLEP VGHKTTSNNF TYSRTKLKCP SPDSPYLYTL 1400
RNSRLLPDQA EADPTVLYWA VPVAAGAGLL IGILAMVAGR GMRCRPREDP 1450
PLSLSTGL 1458
Length:1,458
Mass (Da):161,344
Last modified:February 1, 1994 - v1
Checksum:i9D1608F47B1062E6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z12841 mRNA. Translation: CAA78303.1.
PIRiA45665.
RefSeqiNP_001095178.1. NM_001101708.1.
UniGeneiOcu.2089.

Genome annotation databases

GeneIDi100009309.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z12841 mRNA. Translation: CAA78303.1 .
PIRi A45665.
RefSeqi NP_001095178.1. NM_001101708.1.
UniGenei Ocu.2089.

3D structure databases

ProteinModelPortali Q05017.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000013064.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100009309.

Organism-specific databases

CTDi 151056.

Phylogenomic databases

eggNOGi NOG311176.
HOGENOMi HOG000115574.
HOVERGENi HBG108263.

Family and domain databases

Gene3Di 3.40.50.1110. 6 hits.
InterProi IPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view ]
Pfami PF00657. Lipase_GDSL. 3 hits.
[Graphical view ]
PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Messenger RNAs expressed in intestine of adult but not baby rabbits. Isolation of cognate cDNAs and characterization of a novel brush border protein with esterase and phospholipase activity."
    Boll W., Schmid-Chanda T., Semenza G., Mantei N.
    J. Biol. Chem. 268:12901-12911(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Intestine.

Entry informationi

Entry nameiPLB1_RABIT
AccessioniPrimary (citable) accession number: Q05017
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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