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Q05017 (PLB1_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phospholipase B1, membrane-associated

Short name=Phospholipase B
Alternative name(s):
Phospholipase AdRab-B
Phospholipase B/lipase
Short name=PLB/LIP

Including the following 2 domains:

  1. Phospholipase A2
    EC=3.1.1.4
  2. Lysophospholipase
    EC=3.1.1.5
Gene names
Name:PLB1
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length1458 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Can convert phosphatidylcholine to fatty acids and glycerophosphocholine. Could be involved in uptake of dietary lipids, possibly including long chain retinyl esters. Ref.1

Catalytic activity

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Subcellular location

Apical cell membrane; Single-pass type I membrane protein By similarity. Note: Present in the intestinal brush border membranes.

Tissue specificity

Intestine. Ref.1

Developmental stage

Expressed in the intestine of adult but not baby rabbits. Ref.1

Domain

Repeat 2 contains the catalytic domain By similarity.

Post-translational modification

Undergoes proteolytic cleavage in the ileum By similarity.

Sequence similarities

Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
   Cellular componentCell membrane
Membrane
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentapical plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionlysophospholipase activity

Inferred from electronic annotation. Source: UniProtKB-EC

phospholipase A2 activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 14581439Phospholipase B1, membrane-associated
PRO_0000017854

Regions

Topological domain20 – 14151396Extracellular Potential
Transmembrane1416 – 143621Helical; Potential
Topological domain1437 – 145822Cytoplasmic Potential
Repeat39 – 3473091
Repeat362 – 7073462
Repeat708 – 10543473
Repeat1064 – 14033404
Region39 – 140313654 X 308-326 AA approximate repeats

Sites

Active site4001Nucleophile By similarity
Active site13471 By similarity
Active site13501 By similarity
Binding site4741Substrate; via amide nitrogen By similarity
Binding site5131Substrate By similarity

Amino acid modifications

Glycosylation4931N-linked (GlcNAc...) Potential
Glycosylation5291N-linked (GlcNAc...) Potential
Glycosylation5901N-linked (GlcNAc...) Potential
Glycosylation6901N-linked (GlcNAc...) Potential
Glycosylation7831N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q05017 [UniParc].

Last modified February 1, 1994. Version 1.
Checksum: 9D1608F47B1062E6

FASTA1,458161,344
        10         20         30         40         50         60 
MALWPSVFLL GLLPLLGRGA DQIQTSSGKN TLEGQLWPES LKTFPFPCDP KTLAESVPSE 

        70         80         90        100        110        120 
SVHSLRPSDI KFVAAIGNVE TAPDSGADDL EEQDGTEKRP EQACMGVVTV LSDIIGRFSP 

       130        140        150        160        170        180 
SALMPLCPET RLVPRGGAED LWMQATELVR SMRENPQLDF EHDWKLINVF FSNTSQCFPC 

       190        200        210        220        230        240 
PSAQQKGLVL GGMDKLTRTL DYLQQEVPKA FVNLVDLSEL AAFSRWRQGA QLSPAAEPCR 

       250        260        270        280        290        300 
CLRETSQLTK VLTQWSYLEA WDSLLASSKY NTQESFAVVF QPFFYESSLS ALLAEPPLQD 

       310        320        330        340        350        360 
PTTLALSLWN RMMEPIGRKE EPFSEKERKP LRCPTQESPY LFTYRNSGQL TRVSQPQGKL 

       370        380        390        400        410        420 
EVREGTEIRC PDKDPSDSVP TSVHRLKPAD IKVIGAMGDS LTAGNGAGSQ PGNILDVLTQ 

       430        440        450        460        470        480 
YRGLSWSVGG DQNISTVTTL ANILREFNPS LQGFSVGTGR ETTSQAFFNQ AVAGARADGL 

       490        500        510        520        530        540 
IPQAQRLVAL MKNDTRINFQ EDWKIITVFI GGNDLCDFCN DPVRYSPQNF TDNIGTALDI 

       550        560        570        580        590        600 
LHAEIPRAFV NLVKVLEISK LRELYQETKV SCPRMILRSL CPCVLKFDDN STEIASLIET 

       610        620        630        640        650        660 
IKEYQERTQQ LIDSGRYDTR DDFTVVLQPF FEKVNMPKTQ DGLPDNSFFA PDCFHFSSKA 

       670        680        690        700        710        720 
HAHAASALWN NMLEPVGQKT THNDFEGAVN ITCPNQVWPF LSTYKNSVQG FGTWLPCRDR 

       730        740        750        760        770        780 
SPSASPPTSV HALRPADIQV VAALGDSLTA GIGIGSKPND LSDGTTQYRG LSYSSGGDGS 

       790        800        810        820        830        840 
LDNVTTLPNI LRQFNSNLMG FAVGTGDASG TNAFFNQAVP GAKARDLMSQ VQTLVQRMKD 

       850        860        870        880        890        900 
DHRVNFQEDW KVITVQIGAS DLCDYCTDSN LYSAANFYDH LRDALDALHR EVPRALVNLV 

       910        920        930        940        950        960 
DFMNPSVTRQ VFLGNPDKCP VQQASALCNC VLSPRENSYE LARLEALAQA YQSSLRELVE 

       970        980        990       1000       1010       1020 
SGRYDTREDF SVVLQPFFHS IQLPVLQDGR LDTSFFAPDC VHPNQKFHSQ LSRALWRNML 

      1030       1040       1050       1060       1070       1080 
EPLGGKTDAL DLTAAITLTC PTQNEPFLRT FRNSDYTYPS RPAVENWGSD FLCTAWNASR 

      1090       1100       1110       1120       1130       1140 
GVPNSVHELQ PGDIKVVAAL GDSLTLAMGA RPSNSSDPPM FWRGLSWSIG GDGALETHTT 

      1150       1160       1170       1180       1190       1200 
LPNILKKFNP SILGFSTGTL EGTMGLNVAV QGARAQDMPA QARDLVERMR NSPEIDLEKD 

      1210       1220       1230       1240       1250       1260 
WKLVTLFVGG NDLCHFCENP EGSSEGEYVQ HIQQALDVLY EELPRTFVNV VEVMELAGLH 

      1270       1280       1290       1300       1310       1320 
QDQGGRCATL LAAQSHCTCF KYSQSSVEMQ ELKKVNWNLQ SGLSRLSYSH QYVQREDFAV 

      1330       1340       1350       1360       1370       1380 
VVQPFFQNTL VPLNGRGDTD LTFFSDDCFH FSERGHAEMA IALWNNMLEP VGHKTTSNNF 

      1390       1400       1410       1420       1430       1440 
TYSRTKLKCP SPDSPYLYTL RNSRLLPDQA EADPTVLYWA VPVAAGAGLL IGILAMVAGR 

      1450 
GMRCRPREDP PLSLSTGL 

« Hide

References

[1]"Messenger RNAs expressed in intestine of adult but not baby rabbits. Isolation of cognate cDNAs and characterization of a novel brush border protein with esterase and phospholipase activity."
Boll W., Schmid-Chanda T., Semenza G., Mantei N.
J. Biol. Chem. 268:12901-12911(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Intestine.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z12841 mRNA. Translation: CAA78303.1.
PIRA45665.
RefSeqNP_001095178.1. NM_001101708.1.
UniGeneOcu.2089.

3D structure databases

ProteinModelPortalQ05017.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000013064.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100009309.

Organism-specific databases

CTD151056.

Phylogenomic databases

eggNOGNOG311176.
HOGENOMHOG000115574.
HOVERGENHBG108263.

Family and domain databases

Gene3D3.40.50.1110. 6 hits.
InterProIPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
PROSITEPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLB1_RABIT
AccessionPrimary (citable) accession number: Q05017
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families