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Protein

Phospholipase B1, membrane-associated

Gene

PLB1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Can convert phosphatidylcholine to fatty acids and glycerophosphocholine. Could be involved in uptake of dietary lipids, possibly including long chain retinyl esters.1 Publication

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei400NucleophileBy similarity1
Binding sitei474Substrate; via amide nitrogenBy similarity1
Binding sitei513SubstrateBy similarity1
Active sitei1347By similarity1
Active sitei1350By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Chemistry databases

SwissLipidsiSLP:000000630.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Alternative name(s):
Phospholipase AdRab-B
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLB1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 1415ExtracellularSequence analysisAdd BLAST1396
Transmembranei1416 – 1436HelicalSequence analysisAdd BLAST21
Topological domaini1437 – 1458CytoplasmicSequence analysisAdd BLAST22

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001785420 – 1458Phospholipase B1, membrane-associatedAdd BLAST1439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi493N-linked (GlcNAc...)Sequence analysis1
Glycosylationi529N-linked (GlcNAc...)Sequence analysis1
Glycosylationi590N-linked (GlcNAc...)Sequence analysis1
Glycosylationi690N-linked (GlcNAc...)Sequence analysis1
Glycosylationi783N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ05017.

Expressioni

Tissue specificityi

Intestine.1 Publication

Developmental stagei

Expressed in the intestine of adult but not baby rabbits.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013064.

Structurei

3D structure databases

ProteinModelPortaliQ05017.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati39 – 3471Add BLAST309
Repeati362 – 7072Add BLAST346
Repeati708 – 10543Add BLAST347
Repeati1064 – 14034Add BLAST340

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni39 – 14034 X 308-326 AA approximate repeatsAdd BLAST1365

Domaini

Repeat 2 contains the catalytic domain.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3670. Eukaryota.
ENOG4111FH4. LUCA.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiQ05017.
KOiK14621.

Family and domain databases

Gene3Di3.40.50.1110. 6 hits.
InterProiIPR001087. GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 8 hits.
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05017-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALWPSVFLL GLLPLLGRGA DQIQTSSGKN TLEGQLWPES LKTFPFPCDP
60 70 80 90 100
KTLAESVPSE SVHSLRPSDI KFVAAIGNVE TAPDSGADDL EEQDGTEKRP
110 120 130 140 150
EQACMGVVTV LSDIIGRFSP SALMPLCPET RLVPRGGAED LWMQATELVR
160 170 180 190 200
SMRENPQLDF EHDWKLINVF FSNTSQCFPC PSAQQKGLVL GGMDKLTRTL
210 220 230 240 250
DYLQQEVPKA FVNLVDLSEL AAFSRWRQGA QLSPAAEPCR CLRETSQLTK
260 270 280 290 300
VLTQWSYLEA WDSLLASSKY NTQESFAVVF QPFFYESSLS ALLAEPPLQD
310 320 330 340 350
PTTLALSLWN RMMEPIGRKE EPFSEKERKP LRCPTQESPY LFTYRNSGQL
360 370 380 390 400
TRVSQPQGKL EVREGTEIRC PDKDPSDSVP TSVHRLKPAD IKVIGAMGDS
410 420 430 440 450
LTAGNGAGSQ PGNILDVLTQ YRGLSWSVGG DQNISTVTTL ANILREFNPS
460 470 480 490 500
LQGFSVGTGR ETTSQAFFNQ AVAGARADGL IPQAQRLVAL MKNDTRINFQ
510 520 530 540 550
EDWKIITVFI GGNDLCDFCN DPVRYSPQNF TDNIGTALDI LHAEIPRAFV
560 570 580 590 600
NLVKVLEISK LRELYQETKV SCPRMILRSL CPCVLKFDDN STEIASLIET
610 620 630 640 650
IKEYQERTQQ LIDSGRYDTR DDFTVVLQPF FEKVNMPKTQ DGLPDNSFFA
660 670 680 690 700
PDCFHFSSKA HAHAASALWN NMLEPVGQKT THNDFEGAVN ITCPNQVWPF
710 720 730 740 750
LSTYKNSVQG FGTWLPCRDR SPSASPPTSV HALRPADIQV VAALGDSLTA
760 770 780 790 800
GIGIGSKPND LSDGTTQYRG LSYSSGGDGS LDNVTTLPNI LRQFNSNLMG
810 820 830 840 850
FAVGTGDASG TNAFFNQAVP GAKARDLMSQ VQTLVQRMKD DHRVNFQEDW
860 870 880 890 900
KVITVQIGAS DLCDYCTDSN LYSAANFYDH LRDALDALHR EVPRALVNLV
910 920 930 940 950
DFMNPSVTRQ VFLGNPDKCP VQQASALCNC VLSPRENSYE LARLEALAQA
960 970 980 990 1000
YQSSLRELVE SGRYDTREDF SVVLQPFFHS IQLPVLQDGR LDTSFFAPDC
1010 1020 1030 1040 1050
VHPNQKFHSQ LSRALWRNML EPLGGKTDAL DLTAAITLTC PTQNEPFLRT
1060 1070 1080 1090 1100
FRNSDYTYPS RPAVENWGSD FLCTAWNASR GVPNSVHELQ PGDIKVVAAL
1110 1120 1130 1140 1150
GDSLTLAMGA RPSNSSDPPM FWRGLSWSIG GDGALETHTT LPNILKKFNP
1160 1170 1180 1190 1200
SILGFSTGTL EGTMGLNVAV QGARAQDMPA QARDLVERMR NSPEIDLEKD
1210 1220 1230 1240 1250
WKLVTLFVGG NDLCHFCENP EGSSEGEYVQ HIQQALDVLY EELPRTFVNV
1260 1270 1280 1290 1300
VEVMELAGLH QDQGGRCATL LAAQSHCTCF KYSQSSVEMQ ELKKVNWNLQ
1310 1320 1330 1340 1350
SGLSRLSYSH QYVQREDFAV VVQPFFQNTL VPLNGRGDTD LTFFSDDCFH
1360 1370 1380 1390 1400
FSERGHAEMA IALWNNMLEP VGHKTTSNNF TYSRTKLKCP SPDSPYLYTL
1410 1420 1430 1440 1450
RNSRLLPDQA EADPTVLYWA VPVAAGAGLL IGILAMVAGR GMRCRPREDP

PLSLSTGL
Length:1,458
Mass (Da):161,344
Last modified:February 1, 1994 - v1
Checksum:i9D1608F47B1062E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12841 mRNA. Translation: CAA78303.1.
PIRiA45665.
RefSeqiNP_001095178.1. NM_001101708.1.
UniGeneiOcu.2089.

Genome annotation databases

GeneIDi100009309.
KEGGiocu:100009309.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12841 mRNA. Translation: CAA78303.1.
PIRiA45665.
RefSeqiNP_001095178.1. NM_001101708.1.
UniGeneiOcu.2089.

3D structure databases

ProteinModelPortaliQ05017.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013064.

Chemistry databases

SwissLipidsiSLP:000000630.

Proteomic databases

PRIDEiQ05017.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100009309.
KEGGiocu:100009309.

Organism-specific databases

CTDi151056.

Phylogenomic databases

eggNOGiKOG3670. Eukaryota.
ENOG4111FH4. LUCA.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiQ05017.
KOiK14621.

Family and domain databases

Gene3Di3.40.50.1110. 6 hits.
InterProiIPR001087. GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 8 hits.
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLB1_RABIT
AccessioniPrimary (citable) accession number: Q05017
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 5, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.