Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q05017

- PLB1_RABIT

UniProt

Q05017 - PLB1_RABIT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phospholipase B1, membrane-associated

Gene

PLB1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Can convert phosphatidylcholine to fatty acids and glycerophosphocholine. Could be involved in uptake of dietary lipids, possibly including long chain retinyl esters.1 Publication

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei400 – 4001NucleophileBy similarity
Binding sitei474 – 4741Substrate; via amide nitrogenBy similarity
Binding sitei513 – 5131SubstrateBy similarity
Active sitei1347 – 13471By similarity
Active sitei1350 – 13501By similarity

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC
  2. phospholipase A2 activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Alternative name(s):
Phospholipase AdRab-B
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLB1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

Apical cell membrane By similarity; Single-pass type I membrane protein By similarity
Note: Present in the intestinal brush border membranes.

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 14581439Phospholipase B1, membrane-associatedPRO_0000017854Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi493 – 4931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi529 – 5291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi590 – 5901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi690 – 6901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi783 – 7831N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum.By similarity

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Intestine.1 Publication

Developmental stagei

Expressed in the intestine of adult but not baby rabbits.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000013064.

Structurei

3D structure databases

ProteinModelPortaliQ05017.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 14151396ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1437 – 145822CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1416 – 143621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati39 – 3473091Add
BLAST
Repeati362 – 7073462Add
BLAST
Repeati708 – 10543473Add
BLAST
Repeati1064 – 14033404Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni39 – 140313654 X 308-326 AA approximate repeatsAdd
BLAST

Domaini

Repeat 2 contains the catalytic domain.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG311176.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiQ05017.

Family and domain databases

Gene3Di3.40.50.1110. 6 hits.
InterProiIPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q05017-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALWPSVFLL GLLPLLGRGA DQIQTSSGKN TLEGQLWPES LKTFPFPCDP
60 70 80 90 100
KTLAESVPSE SVHSLRPSDI KFVAAIGNVE TAPDSGADDL EEQDGTEKRP
110 120 130 140 150
EQACMGVVTV LSDIIGRFSP SALMPLCPET RLVPRGGAED LWMQATELVR
160 170 180 190 200
SMRENPQLDF EHDWKLINVF FSNTSQCFPC PSAQQKGLVL GGMDKLTRTL
210 220 230 240 250
DYLQQEVPKA FVNLVDLSEL AAFSRWRQGA QLSPAAEPCR CLRETSQLTK
260 270 280 290 300
VLTQWSYLEA WDSLLASSKY NTQESFAVVF QPFFYESSLS ALLAEPPLQD
310 320 330 340 350
PTTLALSLWN RMMEPIGRKE EPFSEKERKP LRCPTQESPY LFTYRNSGQL
360 370 380 390 400
TRVSQPQGKL EVREGTEIRC PDKDPSDSVP TSVHRLKPAD IKVIGAMGDS
410 420 430 440 450
LTAGNGAGSQ PGNILDVLTQ YRGLSWSVGG DQNISTVTTL ANILREFNPS
460 470 480 490 500
LQGFSVGTGR ETTSQAFFNQ AVAGARADGL IPQAQRLVAL MKNDTRINFQ
510 520 530 540 550
EDWKIITVFI GGNDLCDFCN DPVRYSPQNF TDNIGTALDI LHAEIPRAFV
560 570 580 590 600
NLVKVLEISK LRELYQETKV SCPRMILRSL CPCVLKFDDN STEIASLIET
610 620 630 640 650
IKEYQERTQQ LIDSGRYDTR DDFTVVLQPF FEKVNMPKTQ DGLPDNSFFA
660 670 680 690 700
PDCFHFSSKA HAHAASALWN NMLEPVGQKT THNDFEGAVN ITCPNQVWPF
710 720 730 740 750
LSTYKNSVQG FGTWLPCRDR SPSASPPTSV HALRPADIQV VAALGDSLTA
760 770 780 790 800
GIGIGSKPND LSDGTTQYRG LSYSSGGDGS LDNVTTLPNI LRQFNSNLMG
810 820 830 840 850
FAVGTGDASG TNAFFNQAVP GAKARDLMSQ VQTLVQRMKD DHRVNFQEDW
860 870 880 890 900
KVITVQIGAS DLCDYCTDSN LYSAANFYDH LRDALDALHR EVPRALVNLV
910 920 930 940 950
DFMNPSVTRQ VFLGNPDKCP VQQASALCNC VLSPRENSYE LARLEALAQA
960 970 980 990 1000
YQSSLRELVE SGRYDTREDF SVVLQPFFHS IQLPVLQDGR LDTSFFAPDC
1010 1020 1030 1040 1050
VHPNQKFHSQ LSRALWRNML EPLGGKTDAL DLTAAITLTC PTQNEPFLRT
1060 1070 1080 1090 1100
FRNSDYTYPS RPAVENWGSD FLCTAWNASR GVPNSVHELQ PGDIKVVAAL
1110 1120 1130 1140 1150
GDSLTLAMGA RPSNSSDPPM FWRGLSWSIG GDGALETHTT LPNILKKFNP
1160 1170 1180 1190 1200
SILGFSTGTL EGTMGLNVAV QGARAQDMPA QARDLVERMR NSPEIDLEKD
1210 1220 1230 1240 1250
WKLVTLFVGG NDLCHFCENP EGSSEGEYVQ HIQQALDVLY EELPRTFVNV
1260 1270 1280 1290 1300
VEVMELAGLH QDQGGRCATL LAAQSHCTCF KYSQSSVEMQ ELKKVNWNLQ
1310 1320 1330 1340 1350
SGLSRLSYSH QYVQREDFAV VVQPFFQNTL VPLNGRGDTD LTFFSDDCFH
1360 1370 1380 1390 1400
FSERGHAEMA IALWNNMLEP VGHKTTSNNF TYSRTKLKCP SPDSPYLYTL
1410 1420 1430 1440 1450
RNSRLLPDQA EADPTVLYWA VPVAAGAGLL IGILAMVAGR GMRCRPREDP

PLSLSTGL
Length:1,458
Mass (Da):161,344
Last modified:February 1, 1994 - v1
Checksum:i9D1608F47B1062E6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z12841 mRNA. Translation: CAA78303.1.
PIRiA45665.
RefSeqiNP_001095178.1. NM_001101708.1.
UniGeneiOcu.2089.

Genome annotation databases

GeneIDi100009309.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z12841 mRNA. Translation: CAA78303.1 .
PIRi A45665.
RefSeqi NP_001095178.1. NM_001101708.1.
UniGenei Ocu.2089.

3D structure databases

ProteinModelPortali Q05017.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9986.ENSOCUP00000013064.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100009309.

Organism-specific databases

CTDi 151056.

Phylogenomic databases

eggNOGi NOG311176.
HOGENOMi HOG000115574.
HOVERGENi HBG108263.
InParanoidi Q05017.

Family and domain databases

Gene3Di 3.40.50.1110. 6 hits.
InterProi IPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view ]
Pfami PF00657. Lipase_GDSL. 3 hits.
[Graphical view ]
PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Messenger RNAs expressed in intestine of adult but not baby rabbits. Isolation of cognate cDNAs and characterization of a novel brush border protein with esterase and phospholipase activity."
    Boll W., Schmid-Chanda T., Semenza G., Mantei N.
    J. Biol. Chem. 268:12901-12911(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Intestine.

Entry informationi

Entry nameiPLB1_RABIT
AccessioniPrimary (citable) accession number: Q05017
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3