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Q05017

- PLB1_RABIT

UniProt

Q05017 - PLB1_RABIT

Protein

Phospholipase B1, membrane-associated

Gene

PLB1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Feb 1994)
      Previous versions | rss
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    Functioni

    Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Can convert phosphatidylcholine to fatty acids and glycerophosphocholine. Could be involved in uptake of dietary lipids, possibly including long chain retinyl esters.1 Publication

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
    Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei400 – 4001NucleophileBy similarity
    Binding sitei474 – 4741Substrate; via amide nitrogenBy similarity
    Binding sitei513 – 5131SubstrateBy similarity
    Active sitei1347 – 13471By similarity
    Active sitei1350 – 13501By similarity

    GO - Molecular functioni

    1. lysophospholipase activity Source: UniProtKB-EC
    2. phospholipase A2 activity Source: UniProtKB-EC

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipase B1, membrane-associated
    Short name:
    Phospholipase B
    Alternative name(s):
    Phospholipase AdRab-B
    Phospholipase B/lipase
    Short name:
    PLB/LIP
    Including the following 2 domains:
    Phospholipase A2 (EC:3.1.1.4)
    Lysophospholipase (EC:3.1.1.5)
    Gene namesi
    Name:PLB1
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    Apical cell membrane By similarity; Single-pass type I membrane protein By similarity
    Note: Present in the intestinal brush border membranes.

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 14581439Phospholipase B1, membrane-associatedPRO_0000017854Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi493 – 4931N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi529 – 5291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi590 – 5901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi690 – 6901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi783 – 7831N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Undergoes proteolytic cleavage in the ileum.By similarity

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Intestine.1 Publication

    Developmental stagei

    Expressed in the intestine of adult but not baby rabbits.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000013064.

    Structurei

    3D structure databases

    ProteinModelPortaliQ05017.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 14151396ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1437 – 145822CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1416 – 143621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati39 – 3473091Add
    BLAST
    Repeati362 – 7073462Add
    BLAST
    Repeati708 – 10543473Add
    BLAST
    Repeati1064 – 14033404Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni39 – 140313654 X 308-326 AA approximate repeatsAdd
    BLAST

    Domaini

    Repeat 2 contains the catalytic domain.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG311176.
    HOGENOMiHOG000115574.
    HOVERGENiHBG108263.

    Family and domain databases

    Gene3Di3.40.50.1110. 6 hits.
    InterProiIPR001087. Lipase_GDSL.
    IPR008265. Lipase_GDSL_AS.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view]
    PfamiPF00657. Lipase_GDSL. 3 hits.
    [Graphical view]
    PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q05017-1 [UniParc]FASTAAdd to Basket

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    MALWPSVFLL GLLPLLGRGA DQIQTSSGKN TLEGQLWPES LKTFPFPCDP     50
    KTLAESVPSE SVHSLRPSDI KFVAAIGNVE TAPDSGADDL EEQDGTEKRP 100
    EQACMGVVTV LSDIIGRFSP SALMPLCPET RLVPRGGAED LWMQATELVR 150
    SMRENPQLDF EHDWKLINVF FSNTSQCFPC PSAQQKGLVL GGMDKLTRTL 200
    DYLQQEVPKA FVNLVDLSEL AAFSRWRQGA QLSPAAEPCR CLRETSQLTK 250
    VLTQWSYLEA WDSLLASSKY NTQESFAVVF QPFFYESSLS ALLAEPPLQD 300
    PTTLALSLWN RMMEPIGRKE EPFSEKERKP LRCPTQESPY LFTYRNSGQL 350
    TRVSQPQGKL EVREGTEIRC PDKDPSDSVP TSVHRLKPAD IKVIGAMGDS 400
    LTAGNGAGSQ PGNILDVLTQ YRGLSWSVGG DQNISTVTTL ANILREFNPS 450
    LQGFSVGTGR ETTSQAFFNQ AVAGARADGL IPQAQRLVAL MKNDTRINFQ 500
    EDWKIITVFI GGNDLCDFCN DPVRYSPQNF TDNIGTALDI LHAEIPRAFV 550
    NLVKVLEISK LRELYQETKV SCPRMILRSL CPCVLKFDDN STEIASLIET 600
    IKEYQERTQQ LIDSGRYDTR DDFTVVLQPF FEKVNMPKTQ DGLPDNSFFA 650
    PDCFHFSSKA HAHAASALWN NMLEPVGQKT THNDFEGAVN ITCPNQVWPF 700
    LSTYKNSVQG FGTWLPCRDR SPSASPPTSV HALRPADIQV VAALGDSLTA 750
    GIGIGSKPND LSDGTTQYRG LSYSSGGDGS LDNVTTLPNI LRQFNSNLMG 800
    FAVGTGDASG TNAFFNQAVP GAKARDLMSQ VQTLVQRMKD DHRVNFQEDW 850
    KVITVQIGAS DLCDYCTDSN LYSAANFYDH LRDALDALHR EVPRALVNLV 900
    DFMNPSVTRQ VFLGNPDKCP VQQASALCNC VLSPRENSYE LARLEALAQA 950
    YQSSLRELVE SGRYDTREDF SVVLQPFFHS IQLPVLQDGR LDTSFFAPDC 1000
    VHPNQKFHSQ LSRALWRNML EPLGGKTDAL DLTAAITLTC PTQNEPFLRT 1050
    FRNSDYTYPS RPAVENWGSD FLCTAWNASR GVPNSVHELQ PGDIKVVAAL 1100
    GDSLTLAMGA RPSNSSDPPM FWRGLSWSIG GDGALETHTT LPNILKKFNP 1150
    SILGFSTGTL EGTMGLNVAV QGARAQDMPA QARDLVERMR NSPEIDLEKD 1200
    WKLVTLFVGG NDLCHFCENP EGSSEGEYVQ HIQQALDVLY EELPRTFVNV 1250
    VEVMELAGLH QDQGGRCATL LAAQSHCTCF KYSQSSVEMQ ELKKVNWNLQ 1300
    SGLSRLSYSH QYVQREDFAV VVQPFFQNTL VPLNGRGDTD LTFFSDDCFH 1350
    FSERGHAEMA IALWNNMLEP VGHKTTSNNF TYSRTKLKCP SPDSPYLYTL 1400
    RNSRLLPDQA EADPTVLYWA VPVAAGAGLL IGILAMVAGR GMRCRPREDP 1450
    PLSLSTGL 1458
    Length:1,458
    Mass (Da):161,344
    Last modified:February 1, 1994 - v1
    Checksum:i9D1608F47B1062E6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z12841 mRNA. Translation: CAA78303.1.
    PIRiA45665.
    RefSeqiNP_001095178.1. NM_001101708.1.
    UniGeneiOcu.2089.

    Genome annotation databases

    GeneIDi100009309.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z12841 mRNA. Translation: CAA78303.1 .
    PIRi A45665.
    RefSeqi NP_001095178.1. NM_001101708.1.
    UniGenei Ocu.2089.

    3D structure databases

    ProteinModelPortali Q05017.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000013064.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100009309.

    Organism-specific databases

    CTDi 151056.

    Phylogenomic databases

    eggNOGi NOG311176.
    HOGENOMi HOG000115574.
    HOVERGENi HBG108263.

    Family and domain databases

    Gene3Di 3.40.50.1110. 6 hits.
    InterProi IPR001087. Lipase_GDSL.
    IPR008265. Lipase_GDSL_AS.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view ]
    Pfami PF00657. Lipase_GDSL. 3 hits.
    [Graphical view ]
    PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Messenger RNAs expressed in intestine of adult but not baby rabbits. Isolation of cognate cDNAs and characterization of a novel brush border protein with esterase and phospholipase activity."
      Boll W., Schmid-Chanda T., Semenza G., Mantei N.
      J. Biol. Chem. 268:12901-12911(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Intestine.

    Entry informationi

    Entry nameiPLB1_RABIT
    AccessioniPrimary (citable) accession number: Q05017
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3