ID   FSH2_YEAST              Reviewed;         223 AA.
AC   Q05015; D6W047;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   27-MAR-2024, entry version 142.
DE   RecName: Full=Family of serine hydrolases 2;
DE            EC=3.1.-.-;
GN   Name=FSH2; OrderedLocusNames=YMR222C; ORFNames=YM9959.04C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169872;
RA   Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA   Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA   Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA   Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL   Nature 387:90-93(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   FUNCTION.
RX   PubMed=14645503; DOI=10.1074/mcp.m300082-mcp200;
RA   Baxter S.M., Rosenblum J.S., Knutson S., Nelson M.R., Montimurro J.S.,
RA   Di Gennaro J.A., Speir J.A., Burbaum J.J., Fetrow J.S.;
RT   "Synergistic computational and experimental proteomics approaches for more
RT   accurate detection of active serine hydrolases in yeast.";
RL   Mol. Cell. Proteomics 3:209-225(2004).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Serine hydrolase of unknown specificity.
CC       {ECO:0000269|PubMed:14645503}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 1070 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase 3 family. {ECO:0000305}.
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DR   EMBL; Z49939; CAA90193.1; -; Genomic_DNA.
DR   EMBL; BK006946; DAA10121.1; -; Genomic_DNA.
DR   PIR; S57590; S57590.
DR   RefSeq; NP_013949.1; NM_001182729.1.
DR   AlphaFoldDB; Q05015; -.
DR   SMR; Q05015; -.
DR   BioGRID; 35400; 75.
DR   DIP; DIP-5637N; -.
DR   STRING; 4932.YMR222C; -.
DR   ESTHER; yeast-FSH2; FSH1.
DR   MaxQB; Q05015; -.
DR   PaxDb; 4932-YMR222C; -.
DR   PeptideAtlas; Q05015; -.
DR   EnsemblFungi; YMR222C_mRNA; YMR222C; YMR222C.
DR   GeneID; 855262; -.
DR   KEGG; sce:YMR222C; -.
DR   AGR; SGD:S000004835; -.
DR   SGD; S000004835; FSH2.
DR   VEuPathDB; FungiDB:YMR222C; -.
DR   eggNOG; KOG2551; Eukaryota.
DR   GeneTree; ENSGT00390000003541; -.
DR   HOGENOM; CLU_051938_2_2_1; -.
DR   InParanoid; Q05015; -.
DR   OMA; EDMEMPF; -.
DR   OrthoDB; 517134at2759; -.
DR   BioCyc; YEAST:G3O-32903-MONOMER; -.
DR   BioGRID-ORCS; 855262; 2 hits in 10 CRISPR screens.
DR   PRO; PR:Q05015; -.
DR   Proteomes; UP000002311; Chromosome XIII.
DR   RNAct; Q05015; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IBA:GO_Central.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR005645; FSH-like_dom.
DR   PANTHER; PTHR48070; ESTERASE OVCA2; 1.
DR   PANTHER; PTHR48070:SF6; ESTERASE OVCA2; 1.
DR   Pfam; PF03959; FSH1; 1.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Hydrolase; Reference proteome; Serine esterase.
FT   CHAIN           1..223
FT                   /note="Family of serine hydrolases 2"
FT                   /id="PRO_0000212581"
FT   ACT_SITE        110
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        174
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        203
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   223 AA;  24534 MW;  F7BE3BFCED4EC5B4 CRC64;
     MTKNVLMLHG LAQSGDYFAS KTKGFRAEME KLGYKLYYPT APNEFPPADV PDFLGEVIAD
     APGDGENTGV LAWLENDPST GGYFIPQTTI DYLHNYVLEN GPFAGIVGFS QGAGVAGYLA
     TDFNGLLGLT TEEQPPLEFF MAVSGFRFQP QQYQEQYDLH PISVPSLHVQ GELDTITEPA
     KVQGLYNSCT EDSRTLLMHS GGHFVPNSRG FVRKVAQWLQ QLT
//