Q05015 (FSH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Family of serine hydrolases 2
EC=3.1.-.-

Gene names

Name:	FSH2
Ordered Locus Names:	YMR222C
ORF Names:	YM9959.04C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine hydrolase of unknown specificity. Ref.3
Subcellular location	Cytoplasm Ref.4.
Miscellaneous	Present with 1070 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the AB hydrolase 3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Serine esterase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from direct assay Ref.4. Source: SGD
Molecular_function	carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Family of serine hydrolases 2		PRO_0000212581
Sites
	Active site	110	1	Charge relay system By similarity
	Active site	174	1	Charge relay system By similarity
	Active site	203	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05015 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: F7BE3BFCED4EC5B4	FASTA	223	24,534
	10 20 30 40 50 60 MTKNVLMLHG LAQSGDYFAS KTKGFRAEME KLGYKLYYPT APNEFPPADV PDFLGEVIAD 70 80 90 100 110 120 APGDGENTGV LAWLENDPST GGYFIPQTTI DYLHNYVLEN GPFAGIVGFS QGAGVAGYLA 130 140 150 160 170 180 TDFNGLLGLT TEEQPPLEFF MAVSGFRFQP QQYQEQYDLH PISVPSLHVQ GELDTITEPA 190 200 210 220 KVQGLYNSCT EDSRTLLMHS GGHFVPNSRG FVRKVAQWLQ QLT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Synergistic computational and experimental proteomics approaches for more accurate detection of active serine hydrolases in yeast." Baxter S.M., Rosenblum J.S., Knutson S., Nelson M.R., Montimurro J.S., Di Gennaro J.A., Speir J.A., Burbaum J.J., Fetrow J.S. Mol. Cell. Proteomics 3:209-225(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49939 Genomic DNA. Translation: CAA90193.1. BK006946 Genomic DNA. Translation: DAA10121.1.
PIR	S57590.
RefSeq	NP_013949.1. NM_001182729.1.
3D structure databases
ProteinModelPortal	Q05015.
SMR	Q05015. Positions 5-217.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5637N.
MINT	MINT-552116.
STRING	4932.YMR222C.
Proteomic databases
PaxDb	Q05015.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR222C; YMR222C; YMR222C.
GeneID	855262.
KEGG	sce:YMR222C.
Organism-specific databases
CYGD	YMR222c.
SGD	S000004835. FSH2.
Phylogenomic databases
eggNOG	NOG302067.
GeneTree	ENSGT00390000003541.
HOGENOM	HOG000194266.
OMA	YLCEYIT.
OrthoDB	EOG48WGBD.
Gene expression databases
Genevestigator	Q05015.
GermOnline	YMR222C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005645. Serine_hydrolase_FSH. [Graphical view]
Pfam	PF03959. FSH1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978858.

Entry information

Entry name

FSH2_YEAST

Accession

Primary (citable) accession number: Q05015
Secondary accession number(s): D6W047

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program