Reviewed, UniProtKB/Swiss-Prot Q05006 (CDC22_MEDSA)

Names and origin

Protein names

Recommended name:
    Cell division control protein 2 homolog 2
    EC=2.7.11.22

Gene names

Name:

CDC2B

Organism

Medicago sativa (Alfalfa)

Taxonomic identifier

3879 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it By similarity.
Tissue specificity	Found in most organs including root, young leaf, stem, vegetative meristem and flower bud.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Cell division control protein 2 homolog 2		PRO_0000085754
Regions
	Domain	4 – 287	284	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine By similarity
	Modified residue	161	1	Phosphothreonine; by CAK By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05006-1 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: C2E5D6A67AAF2C3D	FASTA	294	33,886
	10 20 30 40 50 60 MEQYEKVEKI GEGTYGVVYK ARDRATNETI ALKKIRLEQE DEGVPSTAIR EISLLKEMQH 70 80 90 100 110 120 RNIVRLQDVV HSEKRLYLVF EYLDLDLKKF MDSSPEFAKD QRQIKMFLYQ ILCGIAYCHS 130 140 150 160 170 180 HRVLHRDLKP QNLLIDRSSN AVKLADFGLA RAFGIPVRTF THEVVTLWYR APEILLGSRH 190 200 210 220 230 240 YSTPVDVWSV GCIFAEMINQ RPLFPGDSEI DELFKIFRIT GTPNEETWPG VTSLPDFKSA 250 260 270 280 290 FPKWPAKDLA TQVPNLEPAG LDLLSSTCRL DPTRRITARG ALEHEYFKDI KFVP

References

[1]	"cdc2MsB, a cognate cdc2 gene from alfalfa, complements the G1/S but not the G2/M transition of budding yeast cdc28 mutants." Hirt H., Pay A., Boegre L., Meskiene I., Heberle-Bors E. Plant J. 4:61-69(1993) [PubMed: 8220475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Stem.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X70707 mRNA. Translation: CAA50038.1.
PIR	S31332.
3D structure databases
HSSP	HSSP built from PDB template 1H06 based on UniProtKB P24941.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.22. 815.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDC22_MEDSA

Accession

Primary (citable) accession number: Q05006

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)