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Q05001

- NCPR_CATRO

UniProt

Q05001 - NCPR_CATRO

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Protein

NADPH--cytochrome P450 reductase

Gene

CPR

Organism
Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactori

FAD.
FMN.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi200 – 23132FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi348 – 35912FADBy similarityAdd
BLAST
Nucleotide bindingi489 – 49911FADBy similarityAdd
BLAST
Nucleotide bindingi567 – 58519NADPBy similarityAdd
BLAST
Nucleotide bindingi661 – 67717NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. iron ion binding Source: InterPro
  3. NADPH-hemoprotein reductase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductase (EC:1.6.2.4)
Short name:
CPR
Short name:
P450R
Gene namesi
Name:CPR
OrganismiCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Taxonomic identifieri4058 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

Subcellular locationi

Endoplasmic reticulum membrane; Peripheral membrane protein
Note: Anchored to the ER membrane by its N-terminal hydrophobic region.

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 714714NADPH--cytochrome P450 reductasePRO_0000167610Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ05001.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini104 – 254151Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini312 – 559248FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000208. P450R. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q05001-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSSSEKLSP FELMSAILKG AKLDGSNSSD SGVAVSPAVM AMLLENKELV
60 70 80 90 100
MILTTSVAVL IGCVVVLIWR RSSGSGKKVV EPPKLIVPKS VVEPEEIDEG
110 120 130 140 150
KKKFTIFFGT QTGTAEGFAK ALAEEAKARY EKAVIKVIDI DDYAADDEEY
160 170 180 190 200
EEKFRKETLA FFILATYGDG EPTDNAARFY KWFVEGNDRG DWLKNLQYGV
210 220 230 240 250
FGLGNRQYEH FNKIAKVVDE KVAEQGGKRI VPLVLGDDDQ CIEDDFAAWR
260 270 280 290 300
ENVWPELDNL LRDEDDTTVS TTYTAAIPEY RVVFPDKSDS LISEANGHAN
310 320 330 340 350
GYANGNTVYD AQHPCRSNVA VRKELHTPAS DRSCTHLDFD IAGTGLSYGT
360 370 380 390 400
GDHVGVYCDN LSETVEEAER LLNLPPETYF SLHADKEDGT PLAGSSLPPP
410 420 430 440 450
FPPCTLRTAL TRYADLLNTP KKSALLALAA YASDPNEADR LKYLASPAGK
460 470 480 490 500
DEYAQSLVAN QRSLLEVMAE FPSAKPPLGV FFAAIAPRLQ PRFYSISSSP
510 520 530 540 550
RMAPSRIHVT CALVYEKTPG GRIHKGVCST WMKNAIPLEE SRDCSWAPIF
560 570 580 590 600
VRQSNFKLPA DPKVPVIMIG PGTGLAPFRG FLQERLALKE EGAELGTAVF
610 620 630 640 650
FFGCRNRKMD YIYEDELNHF LEIGALSELL VAFSREGPTK QYVQHKMAEK
660 670 680 690 700
ASDIWRMISD GAYVYVCGDA KGMARDVHRT LHTIAQEQGS MDSTQAEGFV
710
KNLQMTGRYL RDVW
Length:714
Mass (Da):78,958
Last modified:February 1, 1995 - v1
Checksum:iDBDD9AF41374CF91
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69791 mRNA. Translation: CAA49446.1.
Y09417 Genomic DNA. Translation: CAA70571.1.
PIRiS31502.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X69791 mRNA. Translation: CAA49446.1 .
Y09417 Genomic DNA. Translation: CAA70571.1 .
PIRi S31502.

3D structure databases

ProteinModelPortali Q05001.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000208. P450R. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and characterization of a cDNA clone from Catharanthus roseus encoding NADPH:cytochrome P-450 reductase, an enzyme essential for reactions catalysed by cytochrome P-450 mono-oxygenases in plants."
    Meijer A.H., Lopes Cardoso M.I., Voskuilen J.T., de Waal A., Verpoorte R., Hoge J.H.C.
    Plant J. 4:47-60(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Lopes Cardoso M.T., Meijer A.H., Rueb S., Queiroz Machado J., Memelink J., Hoge J.H.C.
    Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
    Strain: cv. Morning mist.

Entry informationi

Entry nameiNCPR_CATRO
AccessioniPrimary (citable) accession number: Q05001
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3