Reviewed, UniProtKB/Swiss-Prot Q05001 (NCPR_CATRO)

Names and origin

Protein names

Recommended name:
    NADPH--cytochrome P450 reductase
      Short name=CPR
      Short name=P450R
    EC=1.6.2.4

Gene names

Name:

CPR

Organism

Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)

Taxonomic identifier

4058 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Catharanthus

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
Catalytic activity	NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.
Cofactor	FAD. FMN.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N-terminal hydrophobic region.
Sequence similarities	In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro NADPH-hemoprotein reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 714	714	NADPH--cytochrome P450 reductase		PRO_0000167610
Regions
	Domain	104 – 254	151	Flavodoxin-like
	Domain	312 – 559	248	FAD-binding FR-type
	Nucleotide binding	200 – 231	32	FMN By similarity
	Nucleotide binding	348 – 359	12	FAD By similarity
	Nucleotide binding	489 – 499	11	FAD By similarity
	Nucleotide binding	567 – 585	19	NADP By similarity
	Nucleotide binding	661 – 677	17	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q05001-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: DBDD9AF41374CF91	FASTA	714	78,958
	10 20 30 40 50 60 MDSSSEKLSP FELMSAILKG AKLDGSNSSD SGVAVSPAVM AMLLENKELV MILTTSVAVL 70 80 90 100 110 120 IGCVVVLIWR RSSGSGKKVV EPPKLIVPKS VVEPEEIDEG KKKFTIFFGT QTGTAEGFAK 130 140 150 160 170 180 ALAEEAKARY EKAVIKVIDI DDYAADDEEY EEKFRKETLA FFILATYGDG EPTDNAARFY 190 200 210 220 230 240 KWFVEGNDRG DWLKNLQYGV FGLGNRQYEH FNKIAKVVDE KVAEQGGKRI VPLVLGDDDQ 250 260 270 280 290 300 CIEDDFAAWR ENVWPELDNL LRDEDDTTVS TTYTAAIPEY RVVFPDKSDS LISEANGHAN 310 320 330 340 350 360 GYANGNTVYD AQHPCRSNVA VRKELHTPAS DRSCTHLDFD IAGTGLSYGT GDHVGVYCDN 370 380 390 400 410 420 LSETVEEAER LLNLPPETYF SLHADKEDGT PLAGSSLPPP FPPCTLRTAL TRYADLLNTP 430 440 450 460 470 480 KKSALLALAA YASDPNEADR LKYLASPAGK DEYAQSLVAN QRSLLEVMAE FPSAKPPLGV 490 500 510 520 530 540 FFAAIAPRLQ PRFYSISSSP RMAPSRIHVT CALVYEKTPG GRIHKGVCST WMKNAIPLEE 550 560 570 580 590 600 SRDCSWAPIF VRQSNFKLPA DPKVPVIMIG PGTGLAPFRG FLQERLALKE EGAELGTAVF 610 620 630 640 650 660 FFGCRNRKMD YIYEDELNHF LEIGALSELL VAFSREGPTK QYVQHKMAEK ASDIWRMISD 670 680 690 700 710 GAYVYVCGDA KGMARDVHRT LHTIAQEQGS MDSTQAEGFV KNLQMTGRYL RDVW

References

[1]	"Isolation and characterization of a cDNA clone from Catharanthus roseus encoding NADPH:cytochrome P-450 reductase, an enzyme essential for reactions catalysed by cytochrome P-450 mono-oxygenases in plants." Meijer A.H., Lopes Cardoso M.I., Voskuilen J.T., de Waal A., Verpoorte R., Hoge J.H.C. Plant J. 4:47-60(1993) [PubMed: 8220474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Lopes Cardoso M.T., Meijer A.H., Rueb S., Queiroz Machado J., Memelink J., Hoge J.H.C. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. Strain: cv. Morning mist.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X69791 mRNA. Translation: CAA49446.1. Y09417 Genomic DNA. Translation: CAA70571.1.
PIR	S31502.
3D structure databases
HSSP	HSSP built from PDB template 1AMO based on UniProtKB P00388.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.6.2.4. 20471.
Family and domain databases
InterPro	IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin-like. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR015702. NADPH_Cyt_P450_Rdtase. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
PANTHER	PTHR19384:SF17. NADPH_Cyt_Red. 1 hit.
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCPR_CATRO

Accession

Primary (citable) accession number: Q05001

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)