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Q05001 (NCPR_CATRO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADPH--cytochrome P450 reductase

Short name=CPR
Short name=P450R
EC=1.6.2.4
Gene names
Name:CPR
OrganismCatharanthus roseus (Madagascar periwinkle) (Vinca rosea)
Taxonomic identifier4058 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeCatharanthinaeCatharanthus

Protein attributes

Sequence length714 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

Catalytic activity

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.

Cofactor

FAD.

FMN.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N-terminal hydrophobic region.

Sequence similarities

In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
   LigandFAD
Flavoprotein
FMN
NADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionFMN binding

Inferred from electronic annotation. Source: InterPro

NADPH-hemoprotein reductase activity

Inferred from electronic annotation. Source: UniProtKB-EC

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 714714NADPH--cytochrome P450 reductase
PRO_0000167610

Regions

Domain104 – 254151Flavodoxin-like
Domain312 – 559248FAD-binding FR-type
Nucleotide binding200 – 23132FMN By similarity
Nucleotide binding348 – 35912FAD By similarity
Nucleotide binding489 – 49911FAD By similarity
Nucleotide binding567 – 58519NADP By similarity
Nucleotide binding661 – 67717NADP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q05001 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: DBDD9AF41374CF91

FASTA71478,958
        10         20         30         40         50         60 
MDSSSEKLSP FELMSAILKG AKLDGSNSSD SGVAVSPAVM AMLLENKELV MILTTSVAVL 

        70         80         90        100        110        120 
IGCVVVLIWR RSSGSGKKVV EPPKLIVPKS VVEPEEIDEG KKKFTIFFGT QTGTAEGFAK 

       130        140        150        160        170        180 
ALAEEAKARY EKAVIKVIDI DDYAADDEEY EEKFRKETLA FFILATYGDG EPTDNAARFY 

       190        200        210        220        230        240 
KWFVEGNDRG DWLKNLQYGV FGLGNRQYEH FNKIAKVVDE KVAEQGGKRI VPLVLGDDDQ 

       250        260        270        280        290        300 
CIEDDFAAWR ENVWPELDNL LRDEDDTTVS TTYTAAIPEY RVVFPDKSDS LISEANGHAN 

       310        320        330        340        350        360 
GYANGNTVYD AQHPCRSNVA VRKELHTPAS DRSCTHLDFD IAGTGLSYGT GDHVGVYCDN 

       370        380        390        400        410        420 
LSETVEEAER LLNLPPETYF SLHADKEDGT PLAGSSLPPP FPPCTLRTAL TRYADLLNTP 

       430        440        450        460        470        480 
KKSALLALAA YASDPNEADR LKYLASPAGK DEYAQSLVAN QRSLLEVMAE FPSAKPPLGV 

       490        500        510        520        530        540 
FFAAIAPRLQ PRFYSISSSP RMAPSRIHVT CALVYEKTPG GRIHKGVCST WMKNAIPLEE 

       550        560        570        580        590        600 
SRDCSWAPIF VRQSNFKLPA DPKVPVIMIG PGTGLAPFRG FLQERLALKE EGAELGTAVF 

       610        620        630        640        650        660 
FFGCRNRKMD YIYEDELNHF LEIGALSELL VAFSREGPTK QYVQHKMAEK ASDIWRMISD 

       670        680        690        700        710 
GAYVYVCGDA KGMARDVHRT LHTIAQEQGS MDSTQAEGFV KNLQMTGRYL RDVW 

« Hide

References

[1]"Isolation and characterization of a cDNA clone from Catharanthus roseus encoding NADPH:cytochrome P-450 reductase, an enzyme essential for reactions catalysed by cytochrome P-450 mono-oxygenases in plants."
Meijer A.H., Lopes Cardoso M.I., Voskuilen J.T., de Waal A., Verpoorte R., Hoge J.H.C.
Plant J. 4:47-60(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Lopes Cardoso M.T., Meijer A.H., Rueb S., Queiroz Machado J., Memelink J., Hoge J.H.C.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
Strain: cv. Morning mist.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69791 mRNA. Translation: CAA49446.1.
Y09417 Genomic DNA. Translation: CAA70571.1.
PIRS31502.

3D structure databases

ProteinModelPortalQ05001.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR023208. P450R.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFPIRSF000208. P450R. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNCPR_CATRO
AccessionPrimary (citable) accession number: Q05001
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families