Q04ZG6 (CARA_LEPBL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbamoyl-phosphate synthase small chain
EC=6.3.5.5

Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	LBL_2116

Organism

Leptospira borgpetersenii serovar Hardjo-bovis (strain L550) [Complete proteome] [HAMAP]

Taxonomic identifier

355276 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the CarA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_1000138865
Regions
	Domain	176 – 362	187	Glutamine amidotransferase type-1
	Region	1 – 172	172	CPSase HAMAP MF_01209
Sites
	Active site	252	1	Nucleophile By similarity
	Active site	335	1	By similarity
	Active site	337	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04ZG6 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: 7FF0D5ACCBC09CD4	FASTA	363	40,053
	10 20 30 40 50 60 MKAFLVLDNG MILEGESFGY ESESVGEIVF NTSMAGYQEI LTDPSYCNQI ITLTYPMIGN 70 80 90 100 110 120 YGIHPDNMES SKIQASGLIV REYVDLPSNF MSQKTLSKFL KEYQIPAIQG IDTRKLTRYI 130 140 150 160 170 180 RTNGSPNGGI FVAREYSPEF LEKVKSFPGI TGTDLAKVVT TSTKYIFGTH TGKKYKLAVY 190 200 210 220 230 240 DYGVKTNILR LLDAAGFAVT VYPALTPAEE IMKEGTDAFF LSNGPGDPAP LDYAIDATRK 250 260 270 280 290 300 IMEKGYPLFG ICLGHQIIGL SLGKKTEKMK FGHRGGNQPV KNLSTGQVEI TSQNHGFAVI 310 320 330 340 350 360 DDGKSSEPVS FLNLNDNTVE GILKSGYPLL TVQYHPESAP GPNDSRYLFQ KFYDLVETTK RGK

References

[1]

"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential." Bulach D.M., Zuerner R.L., Wilson P., Seemann T., McGrath A., Cullen P.A., Davis J., Johnson M., Kuczek E., Alt D.P., Peterson-Burch B., Coppel R.L., Rood J.I., Davies J.K., Adler B. Proc. Natl. Acad. Sci. U.S.A. 103:14560-14565(2006) [PubMed: 16973745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: L550.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000348 Genomic DNA. Translation: ABJ79529.1.
RefSeq	YP_798462.1. NC_008508.1.
3D structure databases
ProteinModelPortal	Q04ZG6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q04ZG6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4406782.
GenomeReviews	Gene locus LBL_2116 in contig CP000348_GR.
KEGG	lbl:LBL_2116.
PATRIC	22368486. VBILepBor75619_2695.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0505.
HOGENOM	HBG286341.
OMA	GWNDEDS.
ProtClustDB	PRK12564.
Enzyme and pathway databases
BioCyc	LBOR355276:LBL_2116-MONOMER.
Family and domain databases
HAMAP	MF_01209. CPSase_S_chain. [Tree]
InterPro	IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR017926. GATASE_1. [Graphical view]
Gene3D	G3DSA:3.50.30.20. G3DSA:3.50.30.20. 1 hit.
KO	K01956.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
SMART	SM01097. CPSase_sm_chain. 1 hit. [Graphical view]
SUPFAM	SSF52021. CP_synthsmall. 1 hit.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_LEPBL

Accession

Primary (citable) accession number: Q04ZG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 14, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program