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Reviewed, UniProtKB/Swiss-Prot Q04ZG6 (CARA_LEPBL)

Last modified February 9, 2010. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain
Gene names
Name: carA
Ordered Locus Names: LBL_2116
OrganismLeptospira borgpetersenii serovar Hardjo-bovis (strain L550) [Complete proteome] [HAMAP]
Taxonomic identifier355276 [NCBI]
Taxonomic lineageBacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

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Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209

Subunit structure

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209

Sequence similarities

Belongs to the carA family.

Contains 1 glutamine amidotransferase type-1 domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 363363Carbamoyl-phosphate synthase small chain HAMAP MF_01209
PRO_1000138865

Regions

Domain176 – 362187Glutamine amidotransferase type-1
Region1 – 172172CPSase HAMAP MF_01209

Sites

Active site2521Nucleophile By similarity
Active site3351 By similarity
Active site3371 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q04ZG6-1 [UniParc].

Last modified November 14, 2006. Version 1.
Checksum: 7FF0D5ACCBC09CD4

FASTA36340,053
        10         20         30         40         50         60 
MKAFLVLDNG MILEGESFGY ESESVGEIVF NTSMAGYQEI LTDPSYCNQI ITLTYPMIGN 

        70         80         90        100        110        120 
YGIHPDNMES SKIQASGLIV REYVDLPSNF MSQKTLSKFL KEYQIPAIQG IDTRKLTRYI 

       130        140        150        160        170        180 
RTNGSPNGGI FVAREYSPEF LEKVKSFPGI TGTDLAKVVT TSTKYIFGTH TGKKYKLAVY 

       190        200        210        220        230        240 
DYGVKTNILR LLDAAGFAVT VYPALTPAEE IMKEGTDAFF LSNGPGDPAP LDYAIDATRK 

       250        260        270        280        290        300 
IMEKGYPLFG ICLGHQIIGL SLGKKTEKMK FGHRGGNQPV KNLSTGQVEI TSQNHGFAVI 

       310        320        330        340        350        360 
DDGKSSEPVS FLNLNDNTVE GILKSGYPLL TVQYHPESAP GPNDSRYLFQ KFYDLVETTK 


RGK

« Hide

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References

[1]"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential."
Bulach D.M., Zuerner R.L., Wilson P., Seemann T., McGrath A., Cullen P.A., Davis J., Johnson M., Kuczek E., Alt D.P., Peterson-Burch B., Coppel R.L., Rood J.I., Davies J.K., Adler B.
Proc. Natl. Acad. Sci. U.S.A. 103:14560-14565(2006) [PubMed: 16973745] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000348 Genomic DNA. Translation: ABJ79529.1.
RefSeqYP_798462.1.

3D structure databases

SMRQ04ZG6. Positions 1-361.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ04ZG6.

Genome annotation databases

GeneID4406782.
GenomeReviewsGene locus LBL_2116 in contig CP000348_GR.
KEGGlbl:LBL_2116.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0505.
HOGENOMHBG286341.
OMAEADIPFF.

Family and domain databases

HAMAPMF_01209_B. CPSase_S_chain_B.
[Tree]
InterProIPR006220. Anth_synthII.
IPR001317. CarbamoylP_synth_GATase_dom.
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR011702. GATASE.
IPR017926. GATASE_1.
IPR000991. GATase_class1_C.
[Graphical view]
PANTHERPTHR11405:SF4. CarA_synth_small. 1 hit.
PfamPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
PRINTSPR00097. ANTSNTHASEII.
PR00099. CPSGATASE.
PR00096. GATASE.
TIGRFAMsTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCARA_LEPBL
AccessionPrimary (citable) accession number: Q04ZG6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: November 14, 2006
Last modified: February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents