Reviewed, UniProtKB/Swiss-Prot Q04YC8 (THIM_LEPBL)

Names and origin

Protein names

Recommended name:
    Hydroxyethylthiazole kinase
    EC=2.7.1.50
Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
      Short name=Thz kinase
      Short name=TH kinase

Gene names

Name:	thiM
Ordered Locus Names:	LBL_2553

Organism

Leptospira borgpetersenii serovar Hardjo-bovis (strain L550) [Complete proteome] [HAMAP]

Taxonomic identifier

355276 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Pathway	Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-5-(2-hydroxyethyl)-thiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_1000021517
Sites
	Binding site	55	1	Substrate; via amide nitrogen By similarity
	Binding site	130	1	ATP By similarity
	Binding site	176	1	ATP By similarity
	Binding site	203	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04YC8-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: FB4EEF5732113255	FASTA	264	27,495
	10 20 30 40 50 60 MPKHSALERA WPAKEIVEDL LELRKRSPLT HIMTNIVVTN WTANVLLAVG ASPAMVIAEE 70 80 90 100 110 120 EAGEFVKIAN GLLINVGTIT SNDAKAMKIA ATVAHQTNTP WVLDPVAVGA LGFRTETTKK 130 140 150 160 170 180 LLDLKPTVIR GNASEILTLA GIAGKGKGVD STVNSKDALP YAQELSGKTG AVVAVSGEVD 190 200 210 220 230 240 YVTNGKETIE IYGGDPIMTK VTGVGCSLGA LIASFLGIQK DPLRASASAS AVFAIAGSRS 250 260 AKKSNGPGSF AINFIDQLSQ LSIE

References

[1]

"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential." Bulach D.M., Zuerner R.L., Wilson P., Seemann T., McGrath A., Cullen P.A., Davis J., Johnson M., Kuczek E., Alt D.P., Peterson-Burch B., Coppel R.L., Rood J.I., Davies J.K., Adler B. Proc. Natl. Acad. Sci. U.S.A. 103:14560-14565(2006) [PubMed: 16973745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000348 Genomic DNA. Translation: ABJ79917.1.
RefSeq	YP_798850.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4407884.
GenomeReviews	Gene locus LBL_2553 in contig CP000348_GR.
KEGG	lbl:LBL_2553.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q04YC8. ASPVMAH.
Family and domain databases
HAMAP	MF_00228. [Tree]
InterPro	IPR000417. Hyethyz_kinase. IPR011144. Hyethyz_kinsmonf. [Graphical view]
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_LEPBL

Accession

Primary (citable) accession number: Q04YC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)