Reviewed, UniProtKB/Swiss-Prot Q04WX0 (SAHH_LEPBL)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	LBL_4105

Organism

Leptospira borgpetersenii serovar Hardjo-bovis (strain L550) [Complete proteome] [HAMAP]

Taxonomic identifier

355276 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Leptospiraceae › Leptospira

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon compound metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000024730
Regions
	Region	188 – 356	169	NAD binding By similarity
Sites
	Binding site	62	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	191	1	Substrate By similarity
	Binding site	195	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q04WX0-1 [UniParc]. Last modified November 14, 2006. Version 1. Checksum: F4BD8B927CAFC3E1	FASTA	436	48,096
	10 20 30 40 50 60 MSATTQEKGL NYKVKDLSQA EWGRQEIILA EKEMPGLMAL RQEYKGKKPL AGARIAGSLH 70 80 90 100 110 120 MTIQTAVLIE TLTELGAEVR WSSCNIFSTQ DHAAAAIAKA GVPVFAWKGE TEEEYWWCIE 130 140 150 160 170 180 QTLFFGDKGP NMILDDGGDL TAYVHEKYPK LLSEIRGISE ETTTGVKSLY KLLKKGELKV 190 200 210 220 230 240 PAFNVNDSVT KSKFDNLYGC RESLADGIKR ATDVMLAGKV ALVCGFGDVG KGSAASLRNF 250 260 270 280 290 300 GARVIVTEID PICALQASME GYQVLRVEDI IEQVDIVVTA TGNDDIITLE HMKAMKDGAI 310 320 330 340 350 360 LCNIGHFDTE IQMSRLNSEK GVTKKEIKPQ VDKYTFPDGK SIVVLAEGRL VNLGCATGHP 370 380 390 400 410 420 SFVMSCSFTN QVLAQIELYN NKYELGVYTL PKHLDEKVAA LHLEQLGVRL TKLNQKQADY 430 LGVPLNGPFK PENYRY

References

[1]

"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential." Bulach D.M., Zuerner R.L., Wilson P., Seemann T., McGrath A., Cullen P.A., Davis J., Johnson M., Kuczek E., Alt D.P., Peterson-Burch B., Coppel R.L., Rood J.I., Davies J.K., Adler B. Proc. Natl. Acad. Sci. U.S.A. 103:14560-14565(2006) [PubMed: 16973745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000349 Genomic DNA. Translation: ABJ80425.1.
RefSeq	YP_799358.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4409189.
GenomeReviews	Gene locus LBL_4105 in contig CP000349_GR.
KEGG	lbl:LBL_4105.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q04WX0. HMRAMKD.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR000043. Ad_hcy_hydrolase. IPR015878. Ado_hCys_hydrolase_NAD-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.1480. Ad_hcy_hydrolase. 1 hit.
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_LEPBL

Accession

Primary (citable) accession number: Q04WX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 14, 2006
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)